We narrowed to 2,440 results for: pcas9

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pCAS9-mCherry-TUBB

Plasmid

#66942

Purpose

CRISPaint target selector TUBB

Depositor

Veit Hornung

Article

Schmid-Burgk et al Nat Commun. 2016 Jul 28;7:12338. doi: 10

Insert

gRNA TUBB

Available Since

Sept. 23, 2016

Availability

Academic Institutions and Nonprofits only

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pSpCas9(BB)-2A-GFP-G3BP1(1)

Plasmid

#136058

Purpose

G3BP1 gRNA (#1) inserted into the pSpCas9(BB)-2A-GFP plasmid (GTAGTCCCCTGCTGGTCGGGC)

Depositor

Anthony Leung

Article

Fischer et al Mol Cell. 2020 Apr 2;78(1):70-84.e6. doi

Insert

G3BP1 (G3BP1 Human)

Expression

Mammalian

Available Since

Feb. 4, 2020

Availability

Academic Institutions and Nonprofits only

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pFREE2-SpCas9-sgRNA

Plasmid

#179582

Purpose

Expresses spCas9 and gRNA that targets ColE1 plasmids for curing

Depositor

Kevin Solomon

Article

Lee et al ACS Synth Biol. 2022 Jan 21;11(1):53-60.

Insert

Cas9

Expression

Bacterial

Available Since

March 15, 2022

Availability

Academic Institutions and Nonprofits only

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G1333 DddAtox-N–dSpCas9

Plasmid

#157833

Purpose

expresses split DddAtox-Cas9 construct in mammalian cells

Depositor

David Liu

Article

Mok et al Nature. 2020 Jul 8. pii: 10.1038/s41586-

Insert

bpNLS–G1333 DddAtox-N–dSpCas9–UGI–UGI–bpNLS

Expression

Mammalian

Available Since

Aug. 4, 2020

Availability

Academic Institutions and Nonprofits only

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pTrex-b-NLS-hSpCas9

Plasmid

#62543

Purpose

Expression of Cas9 in T. cruzi

Depositor

Rick Tarleton

Article

Peng et al MBio. 2014 Dec 30;6(1). pii: e02097-14.

Insert

NLS-hSpCas9

Use

CRISPR; Trypanosoma cruzi expression

Tags

3xFLAG and NLS

Promoter

Ribo-HX1

Available Since

April 16, 2015

Availability

Academic Institutions and Nonprofits only

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pMA-SpCas9-g10

Plasmid

#80793

Purpose

BsaI-based cloning of SpCas9 gRNA guide sequence, position 10/20/30 in the array

Depositor

Yonglun Luo

Article

Vad-Nielsen et al Cell Mol Life Sci. 2016 May 13.

Insert

CRISPR gRNA expression cassette (for SpCas9)

Use

CRISPR

Tags

na

Expression

Mammalian

Promoter

U6

Available Since

Sept. 13, 2016

Availability

Academic Institutions and Nonprofits only

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pSpCas9_BB_2A-GFP_MAPRE1-gRNA#1

Plasmid

#107726

Purpose

Knock out of EB1 in human cells by CRISPR/Cas9

Depositor

Torsten Wittmann

Article

van Haren et al Nat Cell Biol. 2018 Jan 29. pii: 10.1038

Insert

MAPRE1 gRNA #1 (targets Exon 1) (MAPRE1 Human)

Expression

Mammalian

Promoter

U6

Available Since

April 30, 2018

Availability

Academic Institutions and Nonprofits only

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pX330-NL-DHFR-SpCas9

Plasmid

#124522

Purpose

Expresses SpCas9 fused to DHFR domains on both the N-terminus and an internal loop in mammalian cells

Depositor

Amit Choudhary

Article

Manna et al Angew Chem Int Ed Engl. 2019 May 6;58(19

Insert

NL-DHFR-SpCas9

Use

AAV

Tags

Destabilized domain of E.coli dihydrofolate reduc…

Expression

Mammalian

Promoter

Cbh

Available Since

July 19, 2019

Availability

Academic Institutions and Nonprofits only

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pX330_Actb sgRNA / hSpCas9

Plasmid

#172832

Purpose

Mammalian expression of a sgRNA targeting the intron 1of Actb (Zhong et al, eLife 2021) under the U6 promotor and hSpCas9 under the CAG promotor. This construct is based on pX330.

Depositor

Haining Zhong

Article

Zhong et al Elife. 2021 Jun 8;10. pii: 64911. doi: 1

Insert

sgRNA targeting intron 1 of Actb under the U6 promotor and hSpCas9 under the CAG promotor

Use

CRISPR

Expression

Mammalian

Available Since

Aug. 24, 2021

Availability

Academic Institutions and Nonprofits only

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pSpCas9(BB)-2A-GFP-G3BP2(1)

Plasmid

#136060

Purpose

G3BP2 gRNA (#1) inserted into the pSpCas9(BB)-2A-GFP plasmid (TCATACTAAAATTCGTCATG)

Depositor

Anthony Leung

Article

Fischer et al Mol Cell. 2020 Apr 2;78(1):70-84.e6. doi

Insert

G3BP2 (G3BP2 Human)

Expression

Mammalian

Available Since

April 24, 2020

Availability

Academic Institutions and Nonprofits only

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pCAS9-mCherry-ACTG1

Plasmid

#66943

Purpose

CRISPaint target selector ACTG1

Depositor

Veit Hornung

Article

Schmid-Burgk et al Nat Commun. 2016 Jul 28;7:12338. doi: 10

Insert

gRNA ACTG1

Available Since

Sept. 23, 2016

Availability

Academic Institutions and Nonprofits only

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pCas9-beta-mmr

Plasmid

#169241

Purpose

Cas9, beta, and mutL mutant expression plasmid for introducing chromosomal point mutations

Depositor

Brian Pfleger

Article

Cook et al Metab Eng. 2021 Jun 24;67:112-124. doi:

Inserts

cas9
beta
mutL-E36K

Use

CRISPR and Synthetic Biology

Expression

Bacterial

Mutation

Changed glutamic acid 36 to lysine

Available Since

June 16, 2021

Availability

Academic Institutions and Nonprofits only

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pSpCas9n(BB)-2A-puro-a5 guide B

Plasmid

#194011

Purpose

For CRISPR knockout of integrin alpha5

Depositor

Timothy Springer

Article

Lin et al Cell. 2022 Sep 15;185(19):3533-3550.e27.

Insert

single-guide RNA (a5_guide B)

Use

CRISPR

Available Since

Dec. 19, 2022

Availability

Academic Institutions and Nonprofits only

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pSpCas9n(BB)-2A-puro-aV guide A

Plasmid

#194008

Purpose

For CRISPR knockout of integrin alphaV

Depositor

Timothy Springer

Article

Lin et al Cell. 2022 Sep 15;185(19):3533-3550.e27.

Insert

single-guide RNA (aV_guide A)

Use

CRISPR

Available Since

Dec. 16, 2022

Availability

Academic Institutions and Nonprofits only

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pSpCas9n(BB)-2A-puro-aV guide B

Plasmid

#194009

Purpose

For CRISPR knockout of integrin alphaV

Depositor

Timothy Springer

Article

Lin et al Cell. 2022 Sep 15;185(19):3533-3550.e27.

Insert

single-guide RNA (aV_guide B)

Use

CRISPR

Available Since

Dec. 16, 2022

Availability

Academic Institutions and Nonprofits only

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pSpCas9n(BB)-2A-puro-a5 guide A

Plasmid

#194010

Purpose

For CRISPR knockout of integrin alpha5

Depositor

Timothy Springer

Article

Lin et al Cell. 2022 Sep 15;185(19):3533-3550.e27.

Insert

single-guide RNA (a5_guide A)

Use

CRISPR

Available Since

Dec. 2, 2022

Availability

Academic Institutions and Nonprofits only

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pX330_ACTB-i4 sgRNA / hSpCas9

Plasmid

#172828

Purpose

Mammalian expression of a sgRNA targeting the intron 1 position 4 of ACTB (Zhong et al, eLife 2021) under the U6 promotor and hSpCas9 under the CAG promotor. This construct is based on pX330.

Depositor

Haining Zhong

Article

Zhong et al Elife. 2021 Jun 8;10. pii: 64911. doi: 1

Insert

sgRNA targeting the intron 1 of ACTB under the U6 promotor and hSpCas9 under the CAG promotor

Use

CRISPR

Expression

Mammalian

Available Since

Aug. 25, 2021

Availability

Academic Institutions and Nonprofits only

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BII-gR-BtW-eSpCas9

Plasmid

#133358

Purpose

Piggybac vector for CRISPR/SpCas9 applications (nuclease, base editing, or epigenetic modifications). Provides gRNA and EspCas9 expression

Depositor

Blair Madison

Article

Fischer et al Am J Physiol Gastrointest Liver Physiol.

Insert

eSpCas9

Tags

Flag-tagged eSpCas9

Expression

Mammalian

Mutation

eSpCas9 mutations

Promoter

CMV enhancer and hEf1a (CpG free)

Available Since

Jan. 21, 2020

Availability

Academic Institutions and Nonprofits only

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pCas9–mKate2ps–CgRNA

Plasmid

#64955

Purpose

Control gRNA (GTCAAGGCACTCTTGCCTA)

Depositor

Leonidas Bleris

Article

Moore et al Nucleic Acids Res. 2015 Jan 30;43(2):129

Insert

Cas9–mKate2ps

Expression

Mammalian

Available Since

May 29, 2015

Availability

Academic Institutions and Nonprofits only

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G1397 DddAtox-C–dSpCas9

Plasmid

#157836

Purpose

expresses split DddAtox-Cas9 construct in mammalian cells

Depositor

David Liu

Article

Mok et al Nature. 2020 Jul 8. pii: 10.1038/s41586-

Insert

bpNLS–G1397 DddAtox-C–dSpCas9–UGI–UGI–bpNLS

Use

CRISPR

Available Since

Aug. 4, 2020

Availability

Academic Institutions and Nonprofits only

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