We narrowed to 4,865 results for: pir

1. pIRES-mCerulean-GID1-Cdc42/N12-Cdc42/13C(Q61L)-GAI1-92-mVenus-CAAX

   Plasmid

   #232264

   Purpose

   Express split-Cdc42 fragments that are fused with CIDs and FPs

   Depositor

   Cliff Stains

   Article

   He et al ACS Chem Biol. 2025 Aug 26. doi: 10.1021

   Inserts

   mCerulean-GID1-Cdc42/N12 (CDC42 Human)
   Cdc42/13C(Q61L)-GAI1-92-mVenus-CAAX (CDC42 Human)
   

   Use

   Tags

   mCerulean and mVenus

   Expression

   Mammalian

   Mutation

   Cdc42-Q61L

   Promoter

   CMV

   Available since

   Sept. 4, 2025

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

2. pIRES-mCerulean-GID1-Cdc42/N12-Cdc42/13C(T17N)-GAI1-92-mVenus-CAAX

   Plasmid

   #232265

   Purpose

   Express split-Cdc42 fragments that are fused with CIDs and FPs

   Depositor

   Cliff Stains

   Article

   He et al ACS Chem Biol. 2025 Aug 26. doi: 10.1021

   Inserts

   mCerulean-GID1-Cdc42/N12 (CDC42 Human)
   Cdc42/13C(T17N)-GAI1-92-mVenus-CAAX (CDC42 Human)
   

   Use

   Tags

   mCerulean and mVenus

   Expression

   Mammalian

   Mutation

   Cdc42-T17N

   Promoter

   CMV

   Available since

   Sept. 4, 2025

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

3. pIRES-mCerulean-ABI-RhoA/N12-RhoA/13C(Q63L)-PYL-mVenus-CAAX

   Plasmid

   #232266

   Purpose

   Express split-RhoA fragments that are fused with CIDs and FPs

   Depositor

   Cliff Stains

   Article

   He et al ACS Chem Biol. 2025 Aug 26. doi: 10.1021

   Inserts

   mCerulean-ABI-RhoA/N12 (RHOA Human)
   RhoA/13C(Q63L)-PYL-mVenus-CAAX (RHOA Human)
   

   Use

   Tags

   mCerulean and mVenus

   Expression

   Mammalian

   Mutation

   RhoA-Q63L

   Promoter

   CMV

   Available since

   Sept. 4, 2025

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

4. pIRES-mCerulean-ABI-RhoA/N12-RhoA/13C(T19N)-PYL-mVenus-CAAX

   Plasmid

   #232267

   Purpose

   Express split-RhoA fragments that are fused with CIDs and FPs

   Depositor

   Cliff Stains

   Article

   He et al ACS Chem Biol. 2025 Aug 26. doi: 10.1021

   Inserts

   mCerulean-ABI-RhoA/N12 (RHOA Human)
   RhoA/13C(T19N)-PYL-mVenus-CAAX (RHOA Human)
   

   Use

   Tags

   mCerulean and mVenus

   Expression

   Mammalian

   Mutation

   RhoA-T19N

   Promoter

   CMV

   Available since

   Sept. 4, 2025

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

5. pIRES-mCerulean-FRB-Cdc42/N12-Cdc42/13C(T17N)-FKBP-mVenus-CAAX

   Plasmid

   #214279

   Purpose

   Express split-Cdc42 fragments that are fused with CIDs and FPs

   Depositor

   Cliff Stains

   Article

   He et al Angew Chem Int Ed Engl. 2024 Jul 26:e202

   Inserts

   Split-Cdc42 fragments fused with CIDs and FPs (CDC42 Human)
   Split-Cdc42 fragments fused with CIDs and FPs (CDC42 Human)
   

   Use

   Tags

   mCerulean (on insert 1) and mVenus (on insert 2)

   Expression

   Mammalian

   Mutation

   Cdc42-T17N

   Promoter

   CMV

   Available since

   July 29, 2024

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

6. pIRES-mCerulean-FRB-Rac1/N12-Rac1/13C(Q61L)-FKBP-mVenus-CAAX

   Plasmid

   #214280

   Purpose

   Express split-Rac1 fragments that are fused with CIDs and FPs

   Depositor

   Cliff Stains

   Article

   He et al Angew Chem Int Ed Engl. 2024 Jul 26:e202

   Inserts

   Split-Rac1 fragments fused with CIDs and FPs (RAC1 Human)
   Split-Rac1 fragments fused with CIDs and FPs (RAC1 Human)
   

   Use

   Tags

   mCerulean (on insert 1) and mVenus (on insert 2)

   Expression

   Mammalian

   Mutation

   Rac1-Q61L

   Promoter

   CMV

   Available since

   July 29, 2024

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

7. pIRES-mCerulean-FRB-Rac1/N12-Rac1/13C(T17N)-FKBP-mVenus-CAAX

   Plasmid

   #214281

   Purpose

   Express split-Rac1 fragments that are fused with CIDs and FPs

   Depositor

   Cliff Stains

   Article

   He et al Angew Chem Int Ed Engl. 2024 Jul 26:e202

   Inserts

   Split-Rac1 fragments fused with CIDs and FPs (RAC1 Human)
   Split-Rac1 fragments fused with CIDs and FPs (RAC1 Human)
   

   Use

   Tags

   mCerulean (N terminal on insert 1) and mVenus (on…

   Expression

   Mammalian

   Mutation

   Rac1-T17N

   Promoter

   CMV

   Available since

   July 29, 2024

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

8. pIRES-mCerulean-FRB-RhoA/N12-RhoA/13C(T19N)-FKBP-mVenus-CAAX

   Plasmid

   #214283

   Purpose

   Express split-RhoA fragments that are fused with CIDs and FPs

   Depositor

   Cliff Stains

   Article

   He et al Angew Chem Int Ed Engl. 2024 Jul 26:e202

   Inserts

   Split-RhoA fragments fused with CIDs and FPs (RHOA Human)
   Split-RhoA fragments fused with CIDs and FPs (RHOA Human)
   

   Use

   Tags

   mCerulean (N terminal on insert 1) and mVenus (on…

   Expression

   Mammalian

   Mutation

   RhoA-T19N

   Promoter

   CMV

   Available since

   July 29, 2024

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

9. pIRES-mCerulean-FRB-KRas/N12-KRas/13C(Q61L)-FKBP-mVenus-CAAX

   Plasmid

   #214284

   Purpose

   Express split-KRas fragments that are fused with CIDs and FPs

   Depositor

   Cliff Stains

   Article

   He et al Angew Chem Int Ed Engl. 2024 Jul 26:e202

   Inserts

   Split-KRas fragments fused with CIDs and FPs (KRAS Human)
   Split-KRas fragments fused with CIDs and FPs (KRAS Human)
   

   Use

   Tags

   mCerulean (on insert 1) and mVenus (on insert 2)

   Expression

   Mammalian

   Mutation

   KRas-Q61L

   Promoter

   CMV

   Available since

   July 29, 2024

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

10. pIRES-mCerulean-FRB-KRas/N12-KRas/13C(S17N)-FKBP-mVenus-CAAX

    Plasmid

    #214285

    Purpose

    Express split-KRas fragments that are fused with CIDs and FPs

    Depositor

    Cliff Stains

    Article

    He et al Angew Chem Int Ed Engl. 2024 Jul 26:e202

    Inserts

    Split-KRas fragments fused with CIDs and FPs (KRAS Human)
    Split-KRas fragments fused with CIDs and FPs (KRAS Human)
    

    Use

    Tags

    mCerulean (N terminal on insert 1) and mVenus (on…

    Expression

    Mammalian

    Mutation

    KRas-S17N

    Promoter

    CMV

    Available since

    July 29, 2024

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

11. pIRES-mCerulean-GID1-KRas/N12-KRas/13C(Q61L)-GAI1-92-mVenus-CAAX

    Plasmid

    #214286

    Purpose

    Express split-KRas fragments that are fused with CIDs and FPs

    Depositor

    Cliff Stains

    Article

    He et al Angew Chem Int Ed Engl. 2024 Jul 26:e202

    Inserts

    Split-KRas fragments fused with CIDs and FPs (KRAS Human)
    Split-KRas fragments fused with CIDs and FPs (KRAS Human)
    

    Use

    Tags

    mCerulean (N terminal on insert 1) and mVenus (on…

    Expression

    Mammalian

    Mutation

    KRas-Q61L

    Promoter

    CMV

    Available since

    July 29, 2024

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

12. pIRES-mCerulean-GID1-KRas/N12-KRas/13C(S17N)-GAI1-92-mVenus-CAAX

    Plasmid

    #214287

    Purpose

    Express split-KRas fragments that are fused with CIDs and FPs

    Depositor

    Cliff Stains

    Article

    He et al Angew Chem Int Ed Engl. 2024 Jul 26:e202

    Inserts

    Split-KRas fragments fused with CIDs and FPs (KRAS Human)
    Split-KRas fragments fused with CIDs and FPs (KRAS Human)
    

    Use

    Tags

    mCerulean (on insert 1) and mVenus (on insert 2)

    Expression

    Mammalian

    Mutation

    KRas-S17N

    Promoter

    CMV

    Available since

    July 29, 2024

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

13. C83m

    Plasmid

    #120994

    Purpose

    MoClo golden gate assembly CD part for pir (Initiation factor required for replication of R6K origin plasmids). Please see Supplemental Documents for annotated Genbank file.

    Depositor

    Richard Murray

    Insert

    pir protein required for R6K ori
    

    Use

    Synthetic Biology

    Tags

    Expression

    Mutation

    Promoter

    Available since

    Dec. 4, 2019

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

14. pR6Kg-Omega1

    Plasmid

    #165864

    Purpose

    Omega level R6K_ origin GB Cloning vector, Conditionally replicative ori, requires pir gene expression requires pir gene expression requires EC100D or EC100D116 bacterial strain (DH10B derived)

    Depositor

    Koen Venken

    Article

    Matinyan et al Cell Rep. 2021 Sep 14;36(11):109700. doi

    Type

    Empty backbone

    Use

    Synthetic Biology

    Tags

    Expression

    Mutation

    Promoter

    Available since

    Sept. 14, 2021

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

15. pR6Kg-Alpha1spm

    Plasmid

    #165866

    Purpose

    Modified Alpha level R6K_ origin GB Cloning vector, Conditionally replicative ori, requires pir gene expression requires pir gene expression requires EC100D or EC100D116 bacterial strain (DH10B derived) uses blue-white screening

    Depositor

    Koen Venken

    Article

    Matinyan et al Cell Rep. 2021 Sep 14;36(11):109700. doi

    Type

    Empty backbone

    Use

    Synthetic Biology

    Tags

    Expression

    Mutation

    Promoter

    Available since

    Sept. 14, 2021

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

16. pR6Kg-Omega2

    Plasmid

    #165865

    Purpose

    Omega level R6K_ origin GB Cloning vector, Conditionally replicative ori, requires pir gene expression requires pir gene expression requires EC100D or EC100D116 bacterial strain (DH10B derived) uses blue-white screening

    Depositor

    Koen Venken

    Article

    Matinyan et al Cell Rep. 2021 Sep 14;36(11):109700. doi

    Type

    Empty backbone

    Use

    Synthetic Biology

    Tags

    Expression

    Mutation

    Promoter

    Available since

    Sept. 14, 2021

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

17. pR6Kg-Alpha2

    Plasmid

    #165863

    Purpose

    Alpha level R6K_ origin GB Cloning vector, Conditionally replicative ori, requires pir gene expression requires pir gene expression requires EC100D or EC100D116 bacterial strain (DH10B derived) uses blue-white screening

    Depositor

    Koen Venken

    Article

    Matinyan et al Cell Rep. 2021 Sep 14;36(11):109700. doi

    Type

    Empty backbone

    Use

    Synthetic Biology

    Tags

    Expression

    Mutation

    Promoter

    Available since

    Sept. 14, 2021

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

18. pHLUM

    Plasmid

    #40276

    Depositor

    Markus Ralser

    Article

    Mulleder et al Nat Biotechnol. 2012 Dec 7;30(12):1176-8

    Inserts

    LEU2 (LEU2 Budding Yeast)
    URA3 (URA3 Budding Yeast)
    MET15/17 (MET17 Budding Yeast)
    

    Use

    Tags

    Expression

    Yeast

    Mutation

    Promoter

    endogenous LEU2, endogenous MET15/17, and endogen…

    Available since

    Nov. 8, 2012

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

19. pJUMP21-1A(sfGFP)

    Plasmid

    #126968

    Purpose

    Level 1 vector. sfGFP reporter. Origin R6K (pir conditional).

    Depositor

    Chris French

    Article

    Valenzuela-Ortega et al Synth Biol (Oxf). 2021 Feb 2;6(1):ysab00

    Type

    Empty backbone

    Use

    Synthetic Biology

    Tags

    Expression

    Bacterial

    Mutation

    None

    Promoter

    Available since

    June 27, 2019

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

20. pECT3

    Plasmid

    #75450

    Purpose

    Plasmid that serves as the PCR template to create genomic pdt3 insertions. Plasmid contains the R6K origin of replication so needs pir gene to replicate

    Depositor

    James Collins

    Article

    Cameron et al Nat Biotechnol. 2014 Dec;32(12):1276-81.

    Insert

    pdt3
    

    Use

    Tags

    Expression

    Bacterial

    Mutation

    Promoter

    none

    Available since

    Oct. 5, 2016

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge