4,178 results

1. pLentiCas9-T2A-BFP

   Plasmid

   #78547

   Purpose

   Cas9 protein with BFP

   Depositor

   Roderic Guigo, Rory Johnson

   Article

   Pulido-Quetglas et al PLoS Comput Biol. 2017 Mar 2;13(3):e1005

   Insert

   T2A-BFP
   

   Use

   Lentiviral

   Expression

   Mammalian

   Promoter

   In frame with Cas9

   Available Since

   July 11, 2016

   Availability

   Academic Institutions and Nonprofits only

   

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2. pU6-Sp-pegRNA-RNF2_+5GtoT

   Plasmid

   #135957

   Purpose

   S. pyogenes pegRNA for +5 G to T edit at the RNF2 site of human cells using prime editing

   Depositor

   David Liu

   Article

   Anzalone et al Nature. 2019 Oct 21. pii: 10.1038/s41586

   Insert

   RNF2_5GtoT pegRNA
   

   Expression

   Mammalian

   Mutation

   See manuscript

   Promoter

   U6

   Available Since

   Jan. 2, 2020

   Availability

   Academic Institutions and Nonprofits only

   

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3. mCherry-Vimentin-N-18

   Plasmid

   #55158

   Purpose

   Localization: Intermediate Filaments, Excitation: 587, Emission: 610

   Depositor

   Michael Davidson

   Insert

   Vimentin (VIM Human)
   

   Tags

   mCherry

   Expression

   Mammalian

   Promoter

   CMV

   Available Since

   Sept. 17, 2014

   Availability

   Academic Institutions and Nonprofits only

   

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4. pMK293 (mAID-mCherry2-Hygro)

   Plasmid

   #72831

   Purpose

   C-terminal tagging with mAID-mCherry2 using CRISPR/Cas9

   Depositor

   Masato Kanemaki

   Article

   Natsume et al Cell Rep. 2016 Apr 5;15(1):210-8. doi: 1

   Insert

   mAID-mCherry2-Hygro
   

   Use

   CRISPR

   Tags

   mAID-mCherry2

   Expression

   Mammalian

   Available Since

   Feb. 23, 2016

   Availability

   Academic Institutions and Nonprofits only

   

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5. LZRS-HOTAIR

   Plasmid

   #26110

   Depositor

   Howard Chang

   Article

   Gupta et al Nature. 2010 Apr 15. 464(7291):1071-6.

   Insert

   HOTAIR (HOTAIR Human)
   

   Use

   Retroviral

   Available Since

   Sept. 15, 2010

   Availability

   Academic Institutions and Nonprofits only

   

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6. pAAV-EF1a-Flpo

   Plasmid

   #55637

   Purpose

   Expresses Flpo in Mammalian Cells

   Depositor

   Karl Deisseroth

   Article

   Fenno et al Nat Methods. 2014 Jul;11(7):763-72. doi:

   Has Service

   AAV Retrograde, AAV1, and AAV2

   Insert

   Flpo
   

   Use

   AAV

   Expression

   Mammalian

   Promoter

   Ef1a

   Available Since

   July 22, 2014

   Availability

   Academic Institutions and Nonprofits only

   

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7. pFOS WT-GL3

   Plasmid

   #11983

   Purpose

   Reporter gene with mouse c-fos promoter. Induced by serum and many growth factors in quiescent cells.

   Depositor

   Ron Prywes

   Article

   Cen et al Mol Cell Biol. 2003 Sep . 23(18):6597-60

   Insert

   c-fos promoter (Fos Mouse)
   

   Tags

   Luc

   Expression

   Mammalian

   Available Since

   Aug. 10, 2007

   Availability

   Academic Institutions and Nonprofits only

   

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8. pET-28a-hPKM1

   Plasmid

   #44241

   Depositor

   Lewis Cantley, Matthew Vander Heiden

   Article

   Christofk et al Nature. 2008 Mar 13;452(7184):181-6. doi

   Insert

   Pyruvate Kinase M1 (PKM Human)
   

   Tags

   6xHis

   Expression

   Bacterial

   Promoter

   T7

   Available Since

   April 10, 2013

   Availability

   Academic Institutions and Nonprofits only

   

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9. pFLAG-GATA2

   Plasmid

   #1418

   Depositor

   Gokhan Hotamisligil

   Article

   Tong et al Mol Cell Biol 2005 Jan;25(2):706-15.

   Insert

   GATA-2 (GATA2 Human)
   

   Tags

   FLAG

   Expression

   Mammalian

   Available Since

   March 2, 2007

   Availability

   Academic Institutions and Nonprofits only

   

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10. pLenti CMV/TO GFP-Zeo DEST (719-1)

    Plasmid

    #17431

    Purpose

    3rd gen lentiviral Gateway destination vector, expression, CMV/TO promoter, GFP-Zeo

    Depositor

    Eric Campeau, Paul Kaufman

    Article

    Campeau et al PLoS One. 2009 Aug 6;4(8):e6529.

    Has Service

    Cloning Grade DNA

    Type

    Empty backbone

    Use

    Lentiviral; Destination vector

    Expression

    Mammalian

    Available Since

    Aug. 12, 2009

    Availability

    Academic Institutions and Nonprofits only

    

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11. pBABE puro H-Ras V12

    Plasmid

    #9051

    Purpose

    Retroviral expression of a constitutively active form of H-Ras

    Depositor

    William Hahn

    Insert

    Hras V12 (HRAS Human)
    

    Use

    Retroviral

    Expression

    Mammalian

    Mutation

    G12V, constitutively active

    Available Since

    Nov. 2, 2005

    Availability

    Academic Institutions and Nonprofits only

    

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12. MSCV PIG (Puro IRES GFP)

    Plasmid

    #18751

    Depositor

    Scott Lowe

    Type

    Empty backbone

    Use

    Retroviral

    Expression

    Mammalian

    Available Since

    July 3, 2008

    Availability

    Academic Institutions and Nonprofits only

    

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13. MLM3613

    Plasmid

    #42251

    Purpose

    Expresses Cas9 nuclease (Streptococcus pyogenes) from CMV and T7 promoters

    Depositor

    Keith Joung

    Article

    Hwang et al Nat Biotechnol. 2013 Jan 29. doi: 10.103

    Insert

    Streptococcus pyogenes Cas9
    

    Use

    CRISPR; Zebrafish expression

    Promoter

    CMV

    Available Since

    Jan. 31, 2013

    Availability

    Academic Institutions and Nonprofits only

    

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14. pGP-AAV-syn-jGCaMP7f-WPRE

    Plasmid

    #104488

    Purpose

    AAV-mediated expression of ultrasensitive protein calcium sensor under the Syn promoter

    Depositor

    Douglas Kim, GENIE Project

    Article

    Dana et al Nat Methods. 2019 Jul;16(7):649-657. doi

    Has Service

    AAV PHP.eB, AAV Retrograde, AAV1, AAV8, and AAV9

    Insert

    jGCaMP7f
    

    Use

    AAV

    Tags

    T7 epitope, Xpress tag, 6xHis

    Expression

    Mammalian

    Promoter

    Synapsin

    Available Since

    Dec. 13, 2017

    Availability

    Academic Institutions and Nonprofits only

    

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15. pTriEx-RhoA FLARE.sc Biosensor WT

    Plasmid

    #12150

    Depositor

    Klaus Hahn

    Article

    Pertz et al Nature. 2006 Apr 20. 440(7087):1069-72.

    Insert

    RhoA Biosensor (RHOA Human)
    

    Tags

    6xHis

    Expression

    Bacterial, Insect, and Mamm…

    Available Since

    Aug. 4, 2006

    Availability

    Academic Institutions and Nonprofits only

    

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16. pCMV flag ERR alpha

    Plasmid

    #10975

    Depositor

    Toren Finkel

    Article

    Ichida et al J Biol Chem. 2002 Dec 27. 277(52):50991-

    Insert

    ERR alpha (ESRRA Human)
    

    Tags

    flag

    Expression

    Mammalian

    Available Since

    Nov. 23, 2005

    Availability

    Academic Institutions and Nonprofits only

    

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17. pSIN4-EF2-O2S

    Plasmid

    #21162

    Depositor

    James Thomson

    Article

    Yu et al Science. 2009 Mar 26.

    Insert

    Oct4, Sox2 (SOX2, POU5F1 Human)
    

    Use

    Lentiviral; Reprogramming

    Available Since

    June 9, 2009

    Availability

    Academic Institutions and Nonprofits only

    

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18. pMSCV-FlagMLL-pl-ENL(5613)

    Plasmid

    #20873

    Depositor

    Robert Slany

    Article

    Bach et al Oncogene. 2009 Feb 12. 28(6):815-23.

    Insert

    MLL (KMT2A, MLLT1 Human)
    

    Use

    Retroviral

    Tags

    Flag

    Expression

    Mammalian

    Available Since

    April 21, 2009

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

19. pQC membrane TdTomato IX

    Plasmid

    #37351

    Depositor

    Connie Cepko

    Article

    Beier et al Dev Biol. 2011 May 15;353(2):309-20. Epu

    Insert

    membrane TdTomato
    

    Tags

    Palmitoylation signal*

    Available Since

    July 30, 2012

    Availability

    Academic Institutions and Nonprofits only

    

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20. WT NFAT2

    Plasmid

    #11101

    Depositor

    Anjana Rao

    Article

    Monticelli et al Eur J Immunol. 2002 Oct . 32(10):2971-8.

    Insert

    NFAT2 (Nfatc1 Mouse)
    

    Use

    Retroviral

    Tags

    3xHA and GFP

    Expression

    Mammalian

    Available Since

    April 19, 2006

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge