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Flag-HA-USP39

Plasmid

#22581

Depositor

Wade Harper

Article

Sowa et al Cell. 2009 Jul 23. 138(2):389-403.

Insert

USP39 (USP39 Human)

Use

Retroviral

Tags

FLAG and HA

Expression

Mammalian

Mutation

None

Available Since

Nov. 16, 2009

Availability

Academic Institutions and Nonprofits only
TFORF1282

Plasmid

#143483

Purpose

Lentiviral vector for overexpressing transcription factor ORFs with unique 24-bp barcodes. Barcodes facilitate identification of transcription factors in pooled screens.

Depositor

Feng Zhang

Article

Joung et al Cell. 2023 Jan 5;186(1):209-229.e26. doi

Insert

SALL4 (SALL4 Human)

Use

Lentiviral

Expression

Mammalian

Promoter

EF1a

Available Since

Jan. 5, 2023

Availability

Academic Institutions and Nonprofits only

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mCherry-Tubulin-C-18

Plasmid

#55148

Purpose

Localization: Microtubules, Excitation: 587, Emission: 610

Depositor

Michael Davidson

Article

Fiolka et al Proc Natl Acad Sci U S A. 2012 Apr 3;109

Insert

Tubulin (TUBA1B Human)

Tags

mCherry

Expression

Mammalian

Promoter

CMV

Available Since

Sept. 17, 2014

Availability

Academic Institutions and Nonprofits only

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IKK-2 S177E S181E

Plasmid

#11105

Purpose

Mammalian expression of constitutively active human IKK-2

Depositor

Anjana Rao

Article

Mercurio et al Science. 1997 Oct 31. 278(5339):860-6.

Insert

IKK-2 (IKBKB Human)

Tags

FLAG

Expression

Mammalian

Mutation

S177E and S181E, constitutively active

Available Since

Feb. 14, 2006

Availability

Academic Institutions and Nonprofits only

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TFORF0235

Plasmid

#142488

Purpose

Lentiviral vector for overexpressing transcription factor ORFs with unique 24-bp barcodes. Barcodes facilitate identification of transcription factors in pooled screens.

Depositor

Feng Zhang

Article

Joung et al Cell. 2023 Jan 5;186(1):209-229.e26. doi

Insert

MYCN (MYCN Human)

Use

Lentiviral

Expression

Mammalian

Promoter

EF1a

Available Since

Jan. 5, 2023

Availability

Academic Institutions and Nonprofits only

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pEGFP-TDP-43 (Q331K)

Plasmid

#235488

Purpose

Expression of human TDP-43-EGFP with human ALS-causing Q331K mutation

Depositor

Maxime Rousseaux

Article

Suk et al Mol Neurodegener. 2025 Mar 28;20(1):38.

Insert

TARDBP (TARDBP Human)

Tags

EGFP

Expression

Mammalian

Mutation

Q331K

Available Since

April 30, 2025

Availability

Industry, Academic Institutions, and Nonprofits

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pWPI-N1ICD

Plasmid

#185525

Purpose

Constitutive overexpression of human NOTCH1 intracellular domain

Depositor

Sean Palecek, Eric Shusta

Article

Gastfriend et al Sci Adv. 2024 Feb 2;10(5):eadi1737. doi:

Insert

NOTCH1 intracellular domain (NOTCH1 Human)

Use

Lentiviral

Promoter

EF-1a

Available Since

Feb. 5, 2024

Availability

Academic Institutions and Nonprofits only

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pRetroX-TetOne-Puro-p62

Plasmid

#204548

Purpose

Expresses p62 in a doxycycline-dependent manner in mammalian cells

Depositor

Masaaki Komatsu

Article

Kurusu et al Dev Cell. 2023 May 9:S1534-5807(23)00191

Insert

p62 (SQSTM1 Human)

Use

Retroviral

Expression

Mammalian

Mutation

None

Available Since

Aug. 11, 2023

Availability

Academic Institutions and Nonprofits only

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pHAGE-mKeima-LGALS3

Plasmid

#175780

Purpose

Lentiviral vector expressing N-terminally tagged mKeima-LGALS3 (Lyso-Keima), for quantitative assessment of lysophagic flux

Depositor

Wade Harper

Article

Eapen et al eLife. 2021 Sep 29;10. pii: 72328. doi:

Insert

LGALS3 (LGALS3 Human)

Use

Lentiviral

Available Since

Jan. 24, 2022

Availability

Academic Institutions and Nonprofits only

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drebrin-YFP

Plasmid

#40359

Depositor

Phillip Gordon-Weeks

Article

Geraldo et al Nat Cell Biol. 2008 Oct;10(10):1181-9. E

Insert

drebrin E2 (DBN1 Human)

Use

Chick expression

Tags

eYFP

Promoter

chick B-actin promoter with CMV enhancer

Available Since

March 6, 2013

Availability

Academic Institutions and Nonprofits only

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pIRESpuroN3xFLAG-UFSP2 C302A

Plasmid

#204554

Purpose

Expresses 3xFLAG-tagged UFSP2 C302A in mammalian cells

Depositor

Masaaki Komatsu

Article

Ishimura et al Nat Commun. 2022 Dec 21;13(1):7857. doi:

Insert

UFSP2 (UFSP2 Human)

Expression

Mammalian

Mutation

C302A

Available Since

Aug. 14, 2023

Availability

Academic Institutions and Nonprofits only

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pCMV-hAR

Plasmid

#89078

Purpose

mammalian expression of human androgen receptor in pCMV5

Depositor

Elizabeth Wilson

Article

Lubahn et al Science. 1988 Apr 15;240(4850):327-30.

Insert

human androgen receptor (hAR) full-length (AR Human)

Expression

Mammalian

Promoter

CMV

Available Since

April 5, 2017

Availability

Academic Institutions and Nonprofits only

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TetON-shYAP

Plasmid

#115667

Purpose

Inducible YAP knockdown

Depositor

Joan Massague

Article

Er et al Nat Cell Biol. 2018 Aug;20(8):966-978. d

Insert

YAP shRNA (YAP1 Human)

Use

Lentiviral and RNAi

Expression

Mammalian

Available Since

Oct. 9, 2018

Availability

Academic Institutions and Nonprofits only

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p667-UBC-CDK12-V5_IDG-K

Plasmid

#135245

Purpose

Gateway destination clone of CDK12 (human) tagged with C-terminal V5 for generating protein-proximity networks using proximity-dependent biotinylation proteomics

Depositor

Ben Major

Insert

CDK12-V5 (CDK12 Human)

Use

Lentiviral; Gateway destination

Tags

V5

Expression

Mammalian

Promoter

Ubiquitin

Available Since

April 15, 2020

Availability

Academic Institutions and Nonprofits only

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pET32a-hEGF

Plasmid

#199231

Purpose

Bacterial production of soluble, active target proteins; Nterm thrombin and enterokinase cleavage sites.

Depositor

Nilson Zanchin

Article

Ferreira et al Sci Rep. 2022 Mar 23;12(1):5034. doi: 10

Insert

Epidermal growth factor, partial (EGF Human)

Tags

TrxA-6xHis-S-tag

Expression

Bacterial

Promoter

T7

Available Since

May 26, 2023

Availability

Academic Institutions and Nonprofits only

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AAVS1-Puro CAG FUCCI

Plasmid

#136934

Purpose

donor plasmid for constitutive FUCCI expression in human cells

Depositor

Xiaoping Bao

Article

Chang et al Biotechnol Bioeng. 2020 Apr 11. doi: 10.

Insert

Clover-Geminin(1-110)-IRES-mKO2-Cdt(30-120)

Use

Synthetic Biology

Tags

Clover and mKO2

Expression

Mammalian

Promoter

CAG

Available Since

April 30, 2020

Availability

Academic Institutions and Nonprofits only

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pcDNA3-EphB3-Fc

Plasmid

#200985

Purpose

Mammalian expression plasmid for soluble EphB3 fused to IgG1 Fc

Depositor

Erik Procko

Article

Narayanan et al J Virol. 2023 Nov 30;97(11):e0062123. do

Insert

EphB3 (EPHB3 Human)

Tags

Fc region of human IgG1

Expression

Mammalian

Mutation

Soluble ectodomain of EphB3

Promoter

CMV

Available Since

Oct. 2, 2023

Availability

Academic Institutions and Nonprofits only

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pcDNA3-TLR7-YFP

Plasmid

#13022

Depositor

Doug Golenbock

Insert

TLR7 (TLR7 Human)

Tags

YFP

Expression

Mammalian

Available Since

Nov. 3, 2006

Availability

Academic Institutions and Nonprofits only

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MAC_N_SOX5

Plasmid

#187742

Purpose

MAC-tagged gene expression of human SOX5

Depositor

Markku Varjosalo

Article

Goos et al Nat Commun. 2022 Feb 9;13(1):766. doi: 1

Insert

SOX5 (SOX5 Human)

Expression

Mammalian

Available Since

Aug. 26, 2022

Availability

Academic Institutions and Nonprofits only

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scFv-sfGFP-DNMT3A1

Plasmid

#102278

Purpose

The plasmid encodes a single-chain antibody that binds to the GCN4 peptide from the SunTag system, and is fused to a DNA methyltransferase DNMT3A1

Depositor

Margaret Goodell

Article

Huang et al Genome Biol. 2017 Sep 18;18(1):176. doi:

Insert

DNMT3A1 (DNMT3A Human)

Use

Lentiviral

Tags

scFV-sfGFP

Expression

Mammalian

Available Since

Dec. 8, 2017

Availability

Academic Institutions and Nonprofits only

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