Skip to main content
Addgene
Showing: 4471 - 4500 of 11176 results

  9. pPP378

    Plasmid
    #158797
    Purpose
    pRSF-Duet1 derived plasmid containing C-terminally hexa-histidine tagged dCasΦ-1(D371A) in MCS1 for expression in E. coli and protein purification
    Depositor
    Jennifer Doudna
    Article
    Pausch et al Science. 2020 Jul 17;369(6501):333-337.
    Insert
    dCasPhi-1 (D371A)
    Use
    Tags
    Expression
    Bacterial
    Mutation
    Promoter
    Available since
    Aug. 26, 2020
    Availability
    Academic Institutions and Nonprofits only
    158797-plasmid-map-sequence-id-309517

    Enlarge

  13. pJY3948

    Plasmid
    #113338
    Purpose
    6XHis-tagged 2-pyrone synthase driven by TEF1intron promoter; codon optimized for Yarrowia lipolytica expression
    Depositor
    Suzanne Sandmeyer
    Article
    Yu et al Biotechnol Bioeng. 2018 May 19. doi: 10.
    Insert
    2-pyrone synthase, codon optimized for Y. lipolytica
    Use
    Tags
    6XHis
    Expression
    Yeast
    Mutation
    Please see depositor comments
    Promoter
    Available since
    Oct. 23, 2018
    Availability
    Academic Institutions and Nonprofits only
    113338-plasmid-map-sequence-id-218737

    Enlarge

  27. pPP384

    Plasmid
    #158799
    Purpose
    pRSF-Duet1 derived plasmid containing C-terminally hexa-histidine tagged dCasΦ-3(D413A) in MCS1 for expression in E. coli and protein purification
    Depositor
    Jennifer Doudna
    Article
    Pausch et al Science. 2020 Jul 17;369(6501):333-337.
    Insert
    dCasPhi-3 (D413A)
    Use
    Tags
    Expression
    Bacterial
    Mutation
    Promoter
    Available since
    Aug. 25, 2020
    Availability
    Academic Institutions and Nonprofits only
    158799-plasmid-map-sequence-id-309569

    Enlarge

  30. pXY1-spc-ptb

    Plasmid
    #90489
    Purpose
    pXY1-spc derivative with sceC gene under the control of ptb promoter used for I-SceI-mediated scarless gene modification via allelic exchange in Clostridium
    Depositor
    Sheng Yang
    Article
    Zhang et al J Microbiol Methods. 2015 Jan;108:49-60.
    Insert
    sceC
    Use
    Tags
    Expression
    Bacterial
    Mutation
    Promoter
    Available since
    June 15, 2017
    Availability
    Academic Institutions and Nonprofits only
    90489-plasmid-map-sequence-id-175410

    Enlarge

Showing: 4471 - 4500 of 11176 results