We narrowed to 16,618 results for: OMP;

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pIDS-RESTwt full-length

Plasmid

#109161

Purpose

Encodes human full-length REST/NRSF to be expressed in a baculovirus/insect cell expression system

Depositor

Monika Golas

Publication

Inui et al Protein Sci. 2017 May;26(5):997-1011. do

Insert

human full-length REST/NRSF (REST Human)

Expression

Insect

Promoter

p10

Available since

Jan. 11, 2019

Availability

Academic Institutions and Nonprofits only

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pENTR221 UBR5 ∆UBR

Plasmid

#81063

Purpose

Gateway entry vector encoding full length UBR5 W1235L (∆UBR)

Depositor

Darren Saunders

Publication

Shearer et al Mol Biol Cell. 2018 May 9:mbcE17040248.

Insert

Ubiquitin protein ligase E3 component N-recognin 5 (UBR5) ∆UBR (UBR5 Human)

Use

Cloning

Mutation

W1235L (bp g3704t)

Promoter

N/A

Available since

Jan. 3, 2017

Availability

Academic Institutions and Nonprofits only

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p3E-mTagBFP-CAAX

Plasmid

#75176

Purpose

Membrane targeted TagBFP (blue) fluorophore with stop codon for C-terminal fusions

Depositor

Nicholas Cole

Publication

Don et al Zebrafish. 2016 Sep 15.

Has service

50 µg Transfection-Ready DNA and 100 µg Transfection-Ready DNA

Insert

mTagBFP-CAAX

Use

Three prime entry vector for multisite gateway th…

Promoter

n/a

Available since

Oct. 6, 2016

Availability

Academic Institutions and Nonprofits only

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MSCV-mir20

Plasmid

#24709

Depositor

Lin He

Publication

Olive et al Genes Dev. 2009 Dec 15. 23(24):2839-49.

Insert

mir-20 (MIR20A Mouse, Human)

Use

Retroviral

Expression

Mammalian

Available since

June 9, 2010

Availability

Academic Institutions and Nonprofits only

Cited by

1 Article

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pEGFP-C1-PH-GAP1IP4BP

Plasmid

#20200

Depositor

Robin Irvine

Publication

Hammond et al J Cell Biol. 2009 Jan 26;184(2):297-308.

Insert

PH-GAP1IP4BP (RASA3 Human)

Tags

GFP

Expression

Mammalian

Mutation

Isolated PH/Btk domain

Available since

Feb. 27, 2009

Availability

Academic Institutions and Nonprofits only

Cited by

1 Article

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pHPSE-P6

Plasmid

#185907

Purpose

Engineered Human Heparanase mutant for e.coli expression

Depositor

Colin Jackson

Publication

Whitefield et al RSC Chem Biol. 2022 Feb 15;3(3):341-349.

Inserts

HPSE1 (HPSE Human)
HPSE1 (HPSE Human, Synthetic)

Tags

His-tag

Expression

Bacterial

Promoter

T7

Available since

Aug. 3, 2022

Availability

Academic Institutions and Nonprofits only

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pcDNA3.1(+)-PKAcat-mCherry

Plasmid

#181841

Purpose

mCherry-tagged PKA catalytic subunit for mammalian expression.

Depositor

Jin Zhang

Publication

Zhang et al Cell. 2020 Sep 17;182(6):1531-1544.e15.

Has service

50 µg Transfection-Ready DNA and 100 µg Transfection-Ready DNA

Insert

PKAcat-mCherry (PRKACA Human)

Tags

mCherry

Expression

Mammalian

Promoter

CMV

Available since

July 6, 2022

Availability

Academic Institutions and Nonprofits only

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pENTR221 UBR5 ∆HECT

Plasmid

#81065

Purpose

Gateway entry vector encoding full length UBR5 C2768A (∆HECT)

Depositor

Darren Saunders

Publication

Shearer et al Mol Biol Cell. 2018 May 9:mbcE17040248.

Insert

Ubiquitin protein ligase E3 component N-recognin 5 (UBR5) ∆HECT (UBR5 Human)

Use

Cloning

Mutation

C2768A (bp t8302g and g8303c)

Promoter

N/A

Available since

Jan. 3, 2017

Availability

Academic Institutions and Nonprofits only

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pFastBac TEV SNAPf Prescission TwinStrepII cloning vector with BioBrick PolyPromoter LIC Subcloning (438-SNAP-V3)

Plasmid

#55223

Depositor

Scott Gradia

Publication

Gradia et al Methods Enzymol. 2017;592:1-26. doi: 10.

Type

Empty backbone

Tags

SNAPf and TwinStrepII

Expression

Insect

Available since

July 9, 2014

Availability

Academic Institutions and Nonprofits only

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pcDNA3-ICUE4

Plasmid

#181846

Purpose

Fourth-generation FRET-based Indicator of cAMP Using Epac. Genetically encoded cAMP indicator. Contains high-sensitivity Epac Q270E mutation.

Depositor

Jin Zhang

Publication

Zhang et al Cell. 2020 Sep 17;182(6):1531-1544.e15.

Has service

50 µg Transfection-Ready DNA and 100 µg Transfection-Ready DNA

Insert

ICUE4 (RAPGEF3 Human)

Tags

6xHIS - T7 tag (gene 10 leader) - Xpress (TM) tag…

Expression

Mammalian

Mutation

Glutamine 122 in Epac1 domain mutated to glutamat…

Promoter

CMV

Available since

June 17, 2022

Availability

Academic Institutions and Nonprofits only

Cited by

1 Article

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loxP-Hygro-loxP-HA-mStayGold-Rab11 HR

Plasmid

#229678

Purpose

Homology repair plasmid for endogenous tagging of Rab11 at the N-terminus with monomeric StayGold and an HA epitope tag. Contains a hygromycin resistance cassette for selection of edited cells

Depositor

Francesca Bottanelli

Publication

Stockhammer et al Nat Cell Biol. 2024 Oct 4. doi: 10.1038/

Has service

50 µg Transfection-Ready DNA and 100 µg Transfection-Ready DNA

Insert

genomic sequence Rab11 locus (RAB11A Human)

Use

CRISPR

Tags

monomeric StayGold, HA

Available since

Jan. 3, 2025

Availability

Academic Institutions and Nonprofits only

Cited by

1 Article

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plentiCMV10x-sct-pp65-b2m-HLA-A0201

Plasmid

#196513

Purpose

Express single-chain pMHC with sequencing tag for 10x genomics

Depositor

Howard Chang

Publication

Yu et al Cell. 2022 Dec 22;185(26):4904-4920.e22.

Insert

single chain of a pp65 peptide, b2m and HLA A0201

Use

Lentiviral

Tags

NA

Expression

Mammalian

Mutation

Y84C, 10xgenomics compatible tag

Promoter

CMV

Available since

May 15, 2023

Availability

Academic Institutions and Nonprofits only

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pEF019 MBP-β-catenin-His

Plasmid

#154070

Purpose

Expresses MBP-β-catenin-His (human β-catenin as a fusion protein with MBP and His- tags) in E.coli

Depositor

Jesse Zalatan

Publication

Gavagan et al Cell Syst. 2020 Jun 24;10(6):515-525.e5.

Insert

CTNNB1 (CTNNB1 Human)

Tags

His6 and MBP

Expression

Bacterial

Promoter

Tac

Available since

Jan. 25, 2021

Availability

Academic Institutions and Nonprofits only

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pEGFP-C1-Cep123 (CCDC123/Cep89)

Plasmid

#67787

Purpose

This plasmid encodes EGFP fused to the N-terminus of isoform 1 of Cep123 (CCDC123/Cep89)

Depositor

Michel Bornens

Publication

Sillibourne et al Biol Open. 2013 Apr 9;2(6):535-45. doi:

Has service

50 µg Transfection-Ready DNA and 100 µg Transfection-Ready DNA

Insert

Cep123 (CEP89 Human)

Tags

EGFP

Expression

Mammalian

Promoter

CMV

Available since

Oct. 21, 2015

Availability

Academic Institutions and Nonprofits only

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pSplit-CO-hDib1

Plasmid

#58788

Purpose

Express LucC-hDib1 fusion protein in mammalian cells

Depositor

Hiroyoshi Ariga

Publication

Maita et al Anal Biochem. 2014 Feb 3. pii: S0003-269

Has service

50 µg Transfection-Ready DNA and 100 µg Transfection-Ready DNA

Insert

hDib1 (TXNL4A Human)

Tags

Flag and Luciferase (394-550aa)

Expression

Mammalian

Promoter

CMV

Available since

Aug. 15, 2014

Availability

Academic Institutions and Nonprofits only

Cited by

1 Article

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p6903 PHAGE-B CMV-N-EGFP Myc

Plasmid

#37608

Depositor

Peter Howley

Publication

Martinez-Noel et al Mol Cell Biol. 2012 May 29.

Has service

50 µg Transfection-Ready DNA and 100 µg Transfection-Ready DNA

Insert

Myc (MYC Human)

Tags

EGFP

Expression

Mammalian

Promoter

CMV

Available since

Aug. 8, 2012

Availability

Academic Institutions and Nonprofits only
pCMV Flag cycT2b (P#1718)

Plasmid

#14599

Depositor

Matija Peterlin

Publication

Kurosu et al Gene. 2004 Dec 8. 343(1):173-9.

Has service

50 µg Transfection-Ready DNA and 100 µg Transfection-Ready DNA

Insert

cycT2 (CCNT2 Human)

Tags

Flag

Expression

Mammalian

Mutation

A137T compared to NP_490595.1

Available since

June 30, 2008

Availability

Academic Institutions and Nonprofits only

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SauriABEmax

Plasmid

#135968

Purpose

Expresses ABEmax and nSauriCas9

Depositor

Yongming Wang

Publication

Hu et al PLoS Biol. 2020 Mar 30;18(3):e3000686. d

Type

Empty backbone

Expression

Mammalian

Available since

April 30, 2020

Availability

Academic Institutions and Nonprofits only

Cited by

1 Article

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pUC57-R3R2

Plasmid

#105109

Purpose

create your own 2in1 constructs

Depositor

Christopher Grefen

Publication

Grefen et al Biotechniques. 2012 Oct 2;53(5):311-14.

Type

Empty backbone

Use

Other

Available since

Feb. 22, 2018

Availability

Academic Institutions and Nonprofits only

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pAct:dCas9-VP64

Plasmid

#78901

Purpose

Expresses dCas9-VP64 under actin promoter for SAM CRISPRa in Drosophila cells

Depositor

Norbert Perrimon

Publication

Chavez et al Nat Methods. 2016 May 23. doi: 10.1038/n

Insert

dCas9-VP64

Use

CRISPR

Expression

Insect

Promoter

pActin (Drosophila)

Available since

Oct. 11, 2016

Availability

Academic Institutions and Nonprofits only

Cited by

1 Article

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