We narrowed to 15,859 results for: cas9
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pEPOR1CB0023
Plasmid#117538PurposeLevel1 Golden Gate Cassette: extended stem sgRNA cassette with AtU6-26 terminator for SpCas9, targeting NbPDS gene in N. benthamianaDepositorInsertsgRNA (Sp Cas9) - extended stem with U626 terminator
ExpressionPlantAvailable sinceJan. 7, 2019AvailabilityAcademic Institutions and Nonprofits only -
U6.3>gRNA.f+e
Plasmid#99139PurposeEmpty gRNA scaffold with "Flip" and "Extension" modifications with optimized chicken specific U6 promoterDepositorInsertgRNA
UseCRISPRPromoterchicken U6.3Available sinceAug. 2, 2017AvailabilityAcademic Institutions and Nonprofits onlyCited by -
Nme2Cas9_pMCSG7
Plasmid#119925PurposeNme2Cas9 bacterial expression plasmidDepositorInsertNme2Cas9
TagsNLS, Nucleoplasmin NLS, and SV40 NLSExpressionBacterialPromoterT7Available sinceJan. 23, 2019AvailabilityAcademic Institutions and Nonprofits only -
pCMV-dCas9-mD3A
Plasmid#78257PurposeExpresses dead Cas9 (dCas9) fused to inactive DNMT3A catalytic domain (CD) under the control of the CMV promoterDepositorHas service50 µg Transfection-Ready DNA and 100 µg Transfection-Ready DNAInsertDNMT3A CD aa 598-912 (DNMT3A Human)
UseCRISPRTagsFlag epitope tagExpressionMammalianMutationE756APromoterpCMVAvailable sinceJune 27, 2016AvailabilityAcademic Institutions and Nonprofits onlyCited by -
Cas9m2-VP64
Plasmid#47317PurposeCas9m2 ActivatorDepositorInsertCas9m2-VP64
UseCRISPRAvailable sinceAug. 19, 2013AvailabilityAcademic Institutions and Nonprofits only -
pYPQ141A
Plasmid#69290PurposeGateway entry vector; single gRNA under AtU6 promoterDepositorTypeEmpty backboneUseCRISPRAvailable sinceSept. 14, 2015AvailabilityAcademic Institutions and Nonprofits only -
pLEX307_CR-PDHA2S291D/S293D
Plasmid#115202PurposeLentiviral transduction and expression of CRISPR/Cas9-resistant PDHA2S291D/S293D into any mammalian cell (when using sgRNA 5'-ATGTAATGACGTGATCCGAG-3')DepositorInsertCR (CRISPR/Cas9-resistant)-PDHA2S291D/S293D (PDHA2 Human)
UseLentiviralMutationc.321C>T (silent when translated to protein, c…PromoterEF1alphaAvailable sinceJuly 5, 2022AvailabilityAcademic Institutions and Nonprofits only -
p-dCas9-p300-Hygro
Plasmid#104411Purposetransient expression of dCas9-p300 fusion proteinDepositorHas service50 µg Transfection-Ready DNA and 100 µg Transfection-Ready DNAInsertp300 (EP300 Human)
UseCRISPRExpressionMammalianPromoterCMV promoterAvailable sinceApril 2, 2020AvailabilityAcademic Institutions and Nonprofits only -
pCSD_SpCas9MT2-NLS-3xHA-NLS-SaCas9WT-2xNLS
Plasmid#107321PurposeExpresses SpCas9 (R1333S) fused to SaCas9 in mammalian cellsDepositorInsertSpCas9-SaCas9 fusion
UseCRISPRTagsNLSExpressionMammalianMutationSpCas9 (R1333S) is fused to SaCas9PromoterCMV IE94Available sinceNov. 15, 2018AvailabilityAcademic Institutions and Nonprofits only -
pH-dCas9-PBE
Plasmid#98162PurposeExpresses CRISPR base editor in riceDepositorInsertNLS-APOBEC1-XTEN-dCas9-UGI-NLS
UseRiceExpressionPlantPromoterUbiAvailable sinceJuly 5, 2017AvailabilityAcademic Institutions and Nonprofits only -
gTBEv4
Plasmid#220620Purposevector for encoding an engineered glycosylase-based thymine (T) base editor (gTBEv4) driven by EF1a promoter, guide RNAs compatible with SpCas9 driven by hU6, mCherry driven by CBh promoterDepositorHas service50 µg Transfection-Ready DNA and 100 µg Transfection-Ready DNAInsertnSpCas9(Nterm)-XTEN-hUNG2-XTEN-Cas9(Cterm)
ExpressionMammalianMutationhUNG2(del1-88;Y156A,A214T,Q259A,Y284D), SpCas9(D1…PromoterhU6, EF1aAvailable sinceJune 21, 2024AvailabilityAcademic Institutions and Nonprofits only -
LRG2.1-BFP
Plasmid#120577PurposeLentiviral plasmid to co-express a guide RNA and EBFP.DepositorTypeEmpty backboneUseLentiviralAvailable sinceFeb. 15, 2019AvailabilityAcademic Institutions and Nonprofits only -
BB3cK_pGAP_23*_pPFK300_Cas9
Plasmid#104910PurposehCas9 under control of pPFK300 for direct cloning of HH-sgRNA-HDV PCR products for episomal expression in P. pastoris and selection on G418DepositorInsertsHH - FS23 linker - HDV
human codon-optimized hcas9 sequence fused to a nuclear localization signal (NLS)
UseCRISPRExpressionYeastAvailable sinceApril 8, 2019AvailabilityAcademic Institutions and Nonprofits onlyCited by -
pCAT001
Plasmid#171061PurposePlasmid for expressing HIV-1 envelope glycoproteinDepositorInsertHIV-1 Env
UseLentiviralExpressionMammalianPromoterCAGAvailable sinceJuly 8, 2021AvailabilityAcademic Institutions and Nonprofits only -
pMCSG7-D16A-NmeCas9
Plasmid#71476Purposebacterial pMCSG7 expression vector expressing Nme cas9 nickase with a D16A mutation (RuvC domain) with T7 promoter, N-terminal His tag and TEV siteDepositorInsertpMCSG7-D16A-NmeCas9
Tags6HisExpressionBacterialMutationD16AAvailable sinceFeb. 26, 2016AvailabilityAcademic Institutions and Nonprofits only -
pDNR-SpCas9-Cdt1
Plasmid#80426PurposeUsed for in vitro transcription of SpCas9-Cdt1 mRNA. Cas9 is fused to Cdt1 peptide and is degraded during S/G2 phases of the cell-cycle.DepositorInsertSpCas9-Cdt1
UseCRISPRTagsCdt1Mutationcodon optimized for humanAvailable sinceAug. 15, 2016AvailabilityAcademic Institutions and Nonprofits only -
pcDNA3.1-dCas9-CibN
Plasmid#60552PurposeExpresses dCas9-CibN in mammalian cellsDepositorHas service50 µg Transfection-Ready DNA and 100 µg Transfection-Ready DNAInsertdCas9-CibN
UseCRISPRTagsFLAG and SV40 NLSExpressionMammalianMutationinactivating Cas9 mutations D10A and H840APromoterCMVAvailable sinceJan. 30, 2015AvailabilityAcademic Institutions and Nonprofits onlyCited by
