We narrowed to 3,388 results for: guide rna expression plasmid

1. pOTTC1270 - pAAV Rosa26 gRNA A+B EF1a EGFP

   Plasmid

   #113156

   Purpose

   An AAV vector that expresses guide RNAs targeting rat Rosa26 and expresses EGFP reporter

   Depositor

   Brandon Harvey

   Article

   Back et al Neuron. 2019 Feb 8. pii: S0896-6273(19)3

   Insert

   Two gRNAs for rat Rosa26
   

   Use

   AAV and CRISPR

   Tags

   Expression

   Mammalian

   Mutation

   Promoter

   mU6 and hU6

   Available since

   March 7, 2019

   Availability

   Academic Institutions and Nonprofits only

   

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2. pOTTC1088 - pAAV TH gRNA A+B pair EF1a EGFP

   Plasmid

   #113155

   Purpose

   An AAV vector that expresses guide RNAs targeting rat TH and expresses EGFP reporter

   Depositor

   Brandon Harvey

   Article

   Back et al Neuron. 2019 Feb 8. pii: S0896-6273(19)3

   Insert

   Two gRNAs for rat TH
   

   Use

   AAV and CRISPR

   Tags

   Expression

   Mammalian

   Mutation

   Promoter

   mU6 and hU6

   Available since

   March 7, 2019

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

3. pOTTC1388 - pAAV MANF gRNA A+B EF1a EGFP

   Plasmid

   #113157

   Purpose

   An AAV vector that expresses guide RNAs targeting rat MANF and expresses EGFP reporter

   Depositor

   Brandon Harvey

   Article

   Back et al Neuron. 2019 Feb 8. pii: S0896-6273(19)3

   Insert

   Two gRNAs for rat MANF
   

   Use

   AAV and CRISPR

   Tags

   Expression

   Mammalian

   Mutation

   Promoter

   mU6 and hU6

   Available since

   March 7, 2019

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

4. pAN056

   Plasmid

   #220052

   Purpose

   Reporter plasmid that expresses a a firefly luciferase gene fused to exons 22-27 of the CFTR gene and a synthetic intron (positive control).

   Depositor

   Blake Wiedenheft

   Article

   Nemudraia et al Science. 2024 Apr 25:eadk5518. doi: 10.1

   Insert

   fLuc-CFTR (exons 22-27) (CFTR Human)
   

   Use

   Tags

   Expression

   Mammalian

   Mutation

   Promoter

   Available since

   May 23, 2024

   Availability

   Academic Institutions and Nonprofits only

   

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5. PAJ408

   Plasmid

   #104150

   Purpose

   dCas9 plasmid expressing full length mKate2 mRNA without RBS

   Depositor

   Alfonso Jaramillo

   Article

   Galizi et al CRISPR J. 2020 Oct;3(5):398-408. doi: 10

   Type

   Empty backbone

   Use

   CRISPR

   Tags

   Expression

   Bacterial

   Mutation

   Promoter

   pLtetO-1

   Available since

   Feb. 18, 2021

   Availability

   Academic Institutions and Nonprofits only

   

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6. PAJ389

   Plasmid

   #104147

   Purpose

   dCas9 plasmid expressing 2A sRNA trigger

   Depositor

   Alfonso Jaramillo

   Article

   Galizi et al CRISPR J. 2020 Oct;3(5):398-408. doi: 10

   Type

   Empty backbone

   Use

   CRISPR

   Tags

   Expression

   Bacterial

   Mutation

   Promoter

   pLtetO-1

   Available since

   Feb. 18, 2021

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

7. PAJ390

   Plasmid

   #104148

   Purpose

   dCas9 plasmid expressing 3A sRNA trigger

   Depositor

   Alfonso Jaramillo

   Article

   Galizi et al CRISPR J. 2020 Oct;3(5):398-408. doi: 10

   Type

   Empty backbone

   Use

   CRISPR

   Tags

   Expression

   Bacterial

   Mutation

   Promoter

   pLtetO-1

   Available since

   Feb. 18, 2021

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

8. PAJ469

   Plasmid

   #104146

   Purpose

   Expresses 3A igRNA and Hybrid-promoter sfGFP

   Depositor

   Alfonso Jaramillo

   Article

   Galizi et al CRISPR J. 2020 Oct;3(5):398-408. doi: 10

   Type

   Empty backbone

   Use

   CRISPR

   Tags

   Expression

   Bacterial

   Mutation

   Promoter

   J23119

   Available since

   Aug. 9, 2018

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

9. pOTTC1179 - pAAV (flox-stop) TH gRNA A EF1a eGFP

   Plasmid

   #113158

   Purpose

   An AAV vector that expresses a Cre-dependent guide RNA targeting rat TH and expresses EGFP reporter

   Depositor

   Brandon Harvey

   Article

   Back et al Neuron. 2019 Feb 8. pii: S0896-6273(19)3

   Insert

   gRNA for rat TH
   

   Use

   AAV, CRISPR, and Cre/Lox

   Tags

   Expression

   Mammalian

   Mutation

   Promoter

   mU6

   Available since

   March 7, 2019

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

10. pMB22

    Plasmid

    #223006

    Purpose

    Control plasmid without Cas and Csy4 for Lotus callus assay

    Depositor

    Martin Parniske

    Article

    Bircheneder et al Plant J. 2024 Sep;119(5):2564-2577. doi:

    Insert

    Not applicable, control plasmid
    

    Use

    CRISPR

    Tags

    Expression

    Plant

    Mutation

    Promoter

    Available since

    March 18, 2025

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

11. pMB24

    Plasmid

    #223008

    Purpose

    Control plasmid without Cas and Csy4 for Nicotiana leaf assay

    Depositor

    Martin Parniske

    Article

    Bircheneder et al Plant J. 2024 Sep;119(5):2564-2577. doi:

    Insert

    Not applicable, control plasmid
    

    Use

    CRISPR

    Tags

    Expression

    Plant

    Mutation

    Promoter

    Available since

    March 18, 2025

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

12. pSB1C3-FeGFP

    Plasmid

    #138261

    Purpose

    Donor-eGFP vector to be used in plasmid to plasmid integration reporter assay. eGFP is conditionally expressed when integrated at iCas9-sites on acceptor plasmid

    Depositor

    Xiao Wang

    Article

    Standage-Beier et al CRISPR J. 2019 Aug;2:209-222. doi: 10.10

    Insert

    eGFP
    

    Use

    CRISPR

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    None

    Available since

    March 3, 2020

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

13. FgH1tUTG

    Plasmid

    #70183

    Purpose

    Inducible expression of guide RNA with fluorescent GFP reporter

    Depositor

    Marco Herold

    Article

    Aubrey et al Cell Rep. 2015 Mar 3;10(8):1422-32. doi:

    Insert

    H1-Tet-sgrna cassette
    

    Use

    CRISPR and Lentiviral

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    Available since

    Nov. 6, 2015

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

14. pDECKO-mCherry GFP

    Plasmid

    #78535

    Purpose

    Control plasmid (paired gRNAs against GFP)

    Depositor

    Roderic Guigo, Rory Johnson

    Article

    Pulido-Quetglas et al PLoS Comput Biol. 2017 Mar 2;13(3):e1005

    Insert

    gRNA1+scaffold+H1promoter+gRNA2
    

    Use

    Lentiviral

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    U6 (for expressing sgRNA) and U6 promoter

    Available since

    July 6, 2016

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

15. pDECKO-mCherry MALAT1_Enhancer.1

    Plasmid

    #78536

    Purpose

    Experimental plasmid

    Depositor

    Roderic Guigo, Rory Johnson

    Article

    Pulido-Quetglas et al PLoS Comput Biol. 2017 Mar 2;13(3):e1005

    Insert

    gRNA1+scaffold+H1promoter+gRNA2
    

    Use

    Lentiviral

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    U6 (for expressing sgRNA) and U6 promoter

    Available since

    Sept. 9, 2016

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

16. pDECKO-mCherry MALAT1_Exon.1

    Plasmid

    #78540

    Purpose

    Experimental plasmid

    Depositor

    Roderic Guigo, Rory Johnson

    Article

    Pulido-Quetglas et al PLoS Comput Biol. 2017 Mar 2;13(3):e1005

    Insert

    gRNA1+scaffold+H1promoter+gRNA2
    

    Use

    Lentiviral

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    U6 and U6 (for expressing sgRNA)

    Available since

    July 11, 2016

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

17. pDECKO-mCherry TFRC_B

    Plasmid

    #78544

    Purpose

    Experimental plasmid

    Depositor

    Roderic Guigo, Rory Johnson

    Article

    Pulido-Quetglas et al PLoS Comput Biol. 2017 Mar 2;13(3):e1005

    Insert

    gRNA1+scaffold+H1promoter+gRNA2
    

    Use

    Lentiviral

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    U6 and U6 (for expressing sgRNA)

    Available since

    July 11, 2016

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

18. pDECKO-mCherry MALAT1_Exon.2

    Plasmid

    #78541

    Purpose

    Experimental plasmid

    Depositor

    Roderic Guigo, Rory Johnson

    Article

    Pulido-Quetglas et al PLoS Comput Biol. 2017 Mar 2;13(3):e1005

    Insert

    gRNA1+scaffold+H1promoter+gRNA2
    

    Use

    Lentiviral

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    U6 (for expressing sgRNA) and U6 promoter

    Available since

    July 11, 2016

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

19. pDECKO-mCherry MALAT1_Exon.4

    Plasmid

    #78543

    Purpose

    Experimental plasmid

    Depositor

    Roderic Guigo, Rory Johnson

    Article

    Pulido-Quetglas et al PLoS Comput Biol. 2017 Mar 2;13(3):e1005

    Insert

    gRNA1+scaffold+H1promoter+gRNA2
    

    Use

    Lentiviral

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    U6 and U6 (for expressing sgRNA)

    Available since

    July 11, 2016

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

20. pDECKO-mCherry MALAT1_Enhancer.4

    Plasmid

    #78539

    Purpose

    Experimental plasmid

    Depositor

    Roderic Guigo, Rory Johnson

    Article

    Pulido-Quetglas et al PLoS Comput Biol. 2017 Mar 2;13(3):e1005

    Insert

    gRNA1+scaffold+H1promoter+gRNA2
    

    Use

    Lentiviral

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    U6 and U6 (for expressing sgRNA)

    Available since

    Aug. 17, 2016

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge