165,075 results

1. pFUGW-Pol2-ffLuc2-eGFP

   Plasmid

   #71394

   Purpose

   Lentiviral vector of luciferase-eGFP fusion gene driven by Pol2 promoter

   Depositor

   Glenn Merlino

   Article

   Day et al Pigment Cell Melanoma Res. 2009 Jun;22(3

   Insert

   Pol2-ffLuc2-eGFP
   

   Use

   Lentiviral

   Expression

   Mammalian

   Promoter

   Pol2 (mouse RNA polymerase II gene promtoer)

   Available Since

   Dec. 8, 2015

   Availability

   Academic Institutions and Nonprofits only

   

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2. pcDNA-Flag-Kdm4b-pA

   Plasmid

   #104486

   Purpose

   Express mouse Kdm4b in mammalian cells. It has a T7 promoter for in vitro transcription. Since polyA is encoded on the vector, polyA-tailing is unnecessary.

   Depositor

   Yi Zhang

   Article

   Inoue et al Genes Dev. 2017 Oct 1;31(19):1927-1932.

   Insert

   Kdm4b (Kdm4b Mouse)
   

   Tags

   Flag epitope tag

   Expression

   Mammalian

   Available Since

   Dec. 19, 2017

   Availability

   Academic Institutions and Nonprofits only

   

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3. pET29a-YS14

   Plasmid

   #66890

   Purpose

   Polycistronic coexpression of Xenopus laevis histones (H2A, H2B, H3 and H4)

   Depositor

   Jung-Hyun Min

   Article

   Shim et al Anal Biochem. 2012 Aug 15;427(2):190-2.

   Inserts

   His6-Thrombin-Xenopus laevis histones H2A
   Xenopus laevis histones H2B
   Xenopus laevis histones H3
   Xenopus laevis histones H4-Thrombin
   

   Tags

   H2A, H2B, H3, H4, His6 tag, and Thrombin

   Expression

   Bacterial

   Promoter

   T7

   Available Since

   Sept. 8, 2015

   Availability

   Academic Institutions and Nonprofits only

   

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4. pcDNA3-10His-mStayGold (E138D)

   Plasmid

   #211363

   Purpose

   Mammalian expression of the bright and photostable monomeric StayGold fluorescent protein (E138D). Contains a N-terminal 10xHis tag.

   Depositor

   Mohan Balasubramanian

   Article

   Ivorra-Molla et al Nat Biotechnol. 2023 Dec 11. doi: 10.103

   Insert

   mStayGold
   

   Tags

   10xHis-tag

   Expression

   Mammalian

   Mutation

   E138D

   Promoter

   CMV

   Available Since

   Dec. 20, 2023

   Availability

   Academic Institutions and Nonprofits only

   

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5. OMMGFP 1-10

   Plasmid

   #164119

   Purpose

   Expresses the non-fluorescent GFP1-10 moiety on the cytosolic face of the OMM

   Depositor

   Tito Calì, Marisa Brini

   Article

   Cieri et al Cell Death Differ. 2018 Jun;25(6):1131-1

   Insert

   OMMGFP 1-10
   

   Expression

   Mammalian

   Available Since

   Feb. 2, 2021

   Availability

   Academic Institutions and Nonprofits only

   

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6. pET LIC cloning vector (2A-T)

   Plasmid

   #29665

   Depositor

   Scott Gradia

   Type

   Empty backbone

   Expression

   Bacterial

   Available Since

   June 9, 2011

   Availability

   Academic Institutions and Nonprofits only

   

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7. Mucolipin1-pHcRed C1

   Plasmid

   #62959

   Purpose

   full length human Mucolipin-1 cloned into pHcRed1 C1

   Depositor

   Paul Luzio

   Article

   Pryor et al Traffic. 2006 Oct;7(10):1388-98.

   Insert

   Mucolipin-1 (MCOLN1 Human)
   

   Tags

   HcRed1

   Expression

   Mammalian

   Mutation

   Mucolipin-1 has 1 silent mutation compared to the…

   Promoter

   CMV

   Available Since

   May 28, 2015

   Availability

   Academic Institutions and Nonprofits only

   

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8. pCMV-GenEPi

   Plasmid

   #140236

   Purpose

   Mammalian expression of Piezo1-based fluorescent force sensor GenEPi

   Depositor

   Periklis Pantazis

   Article

   Yaganoglu et al Nat Commun. 2023 Jul 19;14(1):4352. doi:

   Insert

   GenEPi (Piezo1-based fluorescent force sensor) (PIEZO1 Human)
   

   Tags

   GCaMP 6s RS1 EF4

   Expression

   Mammalian

   Promoter

   simian CMV IE94

   Available Since

   July 24, 2023

   Availability

   Academic Institutions and Nonprofits only

   

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9. Mucolipin1 D471-472K-pHcRed C1

   Plasmid

   #62961

   Purpose

   full length human Mucolipin-1 D471/472K cloned into pHcRed1 C1

   Depositor

   Paul Luzio

   Article

   Pryor et al Traffic. 2006 Oct;7(10):1388-98.

   Insert

   Mucolipin-1 (MCOLN1 Human)
   

   Tags

   HcRed

   Expression

   Mammalian

   Mutation

   D471/472K (and two silent mutations T77 ACA inst…

   Promoter

   CMV

   Available Since

   May 28, 2015

   Availability

   Academic Institutions and Nonprofits only

10. pCAG-GFP

    Plasmid

    #11150

    Purpose

    Mammalian expression vector for expression of GFP (CAG promoter)

    Depositor

    Connie Cepko

    Article

    Matsuda et al Proc Natl Acad Sci U S A. 2004 Jan 6. 10

    Has Service

    Cloning Grade DNA

    Insert

    EGFP from Aequorea victoria
    

    Available Since

    Aug. 4, 2006

    Availability

    Academic Institutions and Nonprofits only

    

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11. pcDNA3 RLUC POLIRES FLUC

    Plasmid

    #45642

    Purpose

    Bicistronic reporter plasmid expressing Renilla luciferase and firefly luciferase in mammalian cells.

    Depositor

    Nahum Sonenberg

    Article

    Poulin et al J Biol Chem. 1998 May 29;273(22):14002-7

    Insert

    RLUC - IRES - FLUC
    

    Use

    Luciferase

    Expression

    Mammalian

    Available Since

    June 16, 2014

    Availability

    Academic Institutions and Nonprofits only

    

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12. T7-VEE-OKS-iM

    Plasmid

    #58972

    Purpose

    RNA-based iPSC generation. VEE RNA replicon 3'ORF encoding OCT4, KLF4, SOX2, separated by 2A peptides followed by an IRES then c-myc and a second IRES and PuroR ORF

    Depositor

    Steven Dowdy

    Article

    Yoshioka et al Cell Stem Cell. 2013 Aug 1;13(2):246-54.

    Inserts

    Oct4 (POU5F1 Human)
    Klf4 (KLF4 Human)
    Sox2 (SOX2 Human)
    cMyc (MYC Human)
    

    Use

    Venezuelan equine encephalitis (vee) virus rna re…

    Expression

    Mammalian

    Promoter

    26S subgenomic promoter and IRES

    Available Since

    Oct. 2, 2014

    Availability

    Academic Institutions and Nonprofits only

    

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13. pHRdSV40-dCas9-10xGCN4_v4-P2A-BFP

    Plasmid

    #60903

    Purpose

    Expressed a nuclease dead Cas9 tagged with 10 copies of the GCN4 peptide v4 and BFP. This plasmid is part of the SunTag system for gene activation

    Depositor

    Ron Vale

    Article

    Tanenbaum et al Cell. 2014 Oct 8. pii: S0092-8674(14)012

    Insert

    Cas9 dead
    

    Use

    CRISPR and Lentiviral

    Expression

    Mammalian

    Available Since

    Nov. 18, 2014

    Availability

    Academic Institutions and Nonprofits only

    

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14. pAAV MP-84 mRFP

    Plasmid

    #206926

    Purpose

    The micro promoter MP-84 is an AAV specific strong promoter for gene expression in mammalian cells. The small size of the promoter (84 bp) allows delivery of large genes in AAV.

    Depositor

    Markus Grompe

    Article

    Chai et al Mol Ther Methods Clin Dev. 2023 May 17;2

    Insert

    mRFP
    

    Use

    AAV

    Expression

    Mammalian

    Promoter

    MP-84

    Available Since

    Sept. 14, 2023

    Availability

    Academic Institutions and Nonprofits only

    

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15. pINDUCER21-puro_Gateway-3xFLAG

    Plasmid

    #172981

    Purpose

    Gateway Destination vector for doxycycline-inducible expression of ORF

    Depositor

    Michael Wehr

    Article

    Ma et al Cell Signal. 2024 Jan;113:110917. doi: 1

    Type

    Empty backbone

    Use

    Lentiviral

    Tags

    3xFLAG

    Expression

    Mammalian

    Available Since

    Oct. 11, 2023

    Availability

    Academic Institutions and Nonprofits only

    

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16. pMRX-IP-HaloTag7-LC3

    Plasmid

    #184899

    Purpose

    Stable expression of HaloTag7-LC3 in mammalian cells to measure autophagic flux

    Depositor

    Noboru Mizushima

    Article

    Yim et al Elife. 2022 Aug 8;11. pii: 78923. doi: 1

    Insert

    LC3B
    

    Tags

    HaloTag7

    Expression

    Mammalian

    Available Since

    June 14, 2022

    Availability

    Academic Institutions and Nonprofits only

    

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17. pFUW-TetO-NFATC2

    Plasmid

    #207191

    Purpose

    doxycycline-inducible expression of Human NFATC2 in mammalian cells

    Depositor

    Filipe Pereira

    Article

    Henriques-Oliveira et al Immunity. 58, 1-20. October 2025.

    Insert

    pFUW-TetO-NFATC2
    

    Use

    Lentiviral

    Expression

    Mammalian

    Promoter

    CMV

    Available Since

    Aug. 29, 2025

    Availability

    Academic Institutions and Nonprofits only

    

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18. PB-CAG-mRuby3-Gal8-P2A-Zeo

    Plasmid

    #150815

    Purpose

    Mammalian expression of mRuby3-Galectin8 (Gal8) fusion protein

    Depositor

    Jordan Green

    Article

    Rui et al Sci Adv. 2022 Jan 7;8(1):eabk2855. doi:

    Insert

    mRuby3-Gal8-P2A-Zeo (LGALS8 Synthetic, Human)
    

    Tags

    Gal8 is fused to the c-terminus of mRuby3

    Expression

    Mammalian

    Promoter

    CAG

    Available Since

    Aug. 5, 2020

    Availability

    Academic Institutions and Nonprofits only

    

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19. pcDNA3.1-AMBRA1-3xFLAG

    Plasmid

    #172605

    Purpose

    Expresses 3xFLAG-tagged AMBRA1 in mammalian cells

    Depositor

    Michele Pagano

    Article

    Simoneschi et al Nature. 2021 Apr;592(7856):789-793. doi:

    Insert

    AMBRA1 (AMBRA1 Human)
    

    Tags

    3xFLAG

    Expression

    Mammalian

    Promoter

    CMV

    Available Since

    Aug. 20, 2021

    Availability

    Academic Institutions and Nonprofits only

    

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20. pDARMO_HUWE1_3xFLAG

    Plasmid

    #187155

    Purpose

    mammalian cell expression of HUWE1-3xFLAG

    Depositor

    Eric Fischer

    Article

    Hunkeler et al Mol Cell. 2021 Sep 2;81(17):3468-3480.e7

    Insert

    HUWE1 (HUWE1 Human)
    

    Tags

    3xFLAG

    Expression

    Mammalian

    Available Since

    Aug. 16, 2022

    Availability

    Academic Institutions and Nonprofits only

    

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