We narrowed to 75,831 results for: Rest
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Plasmid#231988PurposeKnockout non-targeting controlDepositorInsertsgRNA with Cas9 and hygromycin resistance
UseCRISPR and LentiviralTagsExpressionMutationPromoterAvailable sinceFeb. 7, 2025AvailabilityAcademic Institutions and Nonprofits only -
pHIV-EF1a-Il1a-ZsGreen
Plasmid#231987PurposeExpress IL-1alpha-ZsGreen fusion proteinDepositorInsertIL-1alpha-ZsGreen fusion construct
UseLentiviralTagsExpressionMutationPromoterAvailable sinceJan. 30, 2025AvailabilityAcademic Institutions and Nonprofits only -
pCMV-3UTR
Plasmid#202550PurposeReady for cloning and heterologous expression of genes in mammalian cells with protein export to cultivation mediaDepositorTypeEmpty backboneUseTagsATG initiation codon followed by an IGKV3 signal …ExpressionMammalianMutationPromoterCMVAvailable sinceAug. 25, 2023AvailabilityAcademic Institutions and Nonprofits only -
pSC5-toxic3
Plasmid#188625PurposepSC5GGv2 with HindII Gene InsertDepositorInsertHindII
UseSynthetic Biology; Diatom expressionTagsExpressionBacterial and YeastMutationACT1 intron insertPromoterAvailable sinceMay 30, 2023AvailabilityAcademic Institutions and Nonprofits only -
pDA2ScIfm
Plasmid#193786PurposeExpresses frame-shifted CI under the control of PLTATAA synthetic promoter, carries a synthetic fragment with additional restriction sites, ColE1 origin of replication, Ampicillin selectionDepositorInsertFrame-shifted CI
UseSynthetic BiologyTagsExpressionBacterialMutationPromoterPLTATAA synthetic promoter carrying binding sites…Available sinceMay 22, 2023AvailabilityAcademic Institutions and Nonprofits only -
pODN-mNG-CfANLN
Plasmid#183837PurposeRepair template for the N-terminal tagging of anillin with mNeonGreen in canine cells using CRISPR/Cas9.DepositorInsertCanis familiaris ANLN homology arms with mNeonGreen-linker
UseCRISPR; Donor templateTagsmNeonGreen-linkerExpressionMammalianMutationHomology arms contain point mutations to remove t…PromoterAvailable sinceMay 23, 2022AvailabilityAcademic Institutions and Nonprofits only -
TMD20
Plasmid#163675PurposeExpresses a ST6GAL1 mutant with 20 amino acids in its TMD in mammalian cellsDepositorInsertpartial length ST6 beta-galactoside alpha-2,6-sialyltransferase 1 mutant (ST6GAL1 Human)
UseTagsGFPExpressionMammalianMutationFor this partial length ST chimera, two restricti…PromoterAvailable sinceNov. 24, 2021AvailabilityAcademic Institutions and Nonprofits only -
TMD21
Plasmid#163676PurposeExpresses a ST6GAL1 mutant with 21 amino acids in its TMD in mammalian cellsDepositorInsertpartial length ST6 beta-galactoside alpha-2,6-sialyltransferase 1 mutant (ST6GAL1 Human)
UseTagsGFPExpressionMammalianMutationFor this partial length ST chimera, two restricti…PromoterAvailable sinceNov. 24, 2021AvailabilityAcademic Institutions and Nonprofits only -
TMD22
Plasmid#163677PurposeExpresses a ST6GAL1 mutant with 22 amino acids in its TMD in mammalian cellsDepositorInsertpartial length ST6 beta-galactoside alpha-2,6-sialyltransferase 1 mutant (ST6GAL1 Human)
UseTagsGFPExpressionMammalianMutationFor this partial length ST chimera, two restricti…PromoterAvailable sinceNov. 24, 2021AvailabilityAcademic Institutions and Nonprofits only -
TMD24
Plasmid#163678PurposeExpresses a ST6GAL1 mutant with 24 amino acids in its TMD in mammalian cellsDepositorInsertpartial length ST6 beta-galactoside alpha-2,6-sialyltransferase 1 mutant (ST6GAL1 Human)
UseTagsGFPExpressionMammalianMutationFor this partial length ST chimera, two restricti…PromoterAvailable sinceNov. 24, 2021AvailabilityAcademic Institutions and Nonprofits only -
Clim Actin MCH Puro (CAMP)
Plasmid#104446PurposeExpresses mCherry under endogenous actin promoter. It can be used to transfect Corallochytrium limacisporum (Corallochytrea) cells and visualize them by fluorescent microscope. Puromycin resistant.DepositorInsertmCherry
UseExpression in corallochytrium (single-celled euka…TagsExpressionMutationPromoteractinAvailable sinceFeb. 16, 2021AvailabilityAcademic Institutions and Nonprofits only -
RAP2A-p2A-E2Crimson
Plasmid#83940PurposeLentiviral vector expressing RAP2A-p2A-E2CrimsonDepositorInsertRAP2A (RAP2A Human)
UseLentiviralTagsp2A-E2CrimsonExpressionMammalianMutationPromoterEF1aAvailable sinceFeb. 1, 2021AvailabilityAcademic Institutions and Nonprofits only -
SLC35B2-p2A-E2Crimson
Plasmid#83941PurposeLentiviral vector expressing SLC35B2-p2A-E2CrimsonDepositorInsertSLC35B2 (SLC35B2 Human)
UseLentiviralTagsp2A-E2CrimsonExpressionMammalianMutationPromoterEF1aAvailable sinceFeb. 1, 2021AvailabilityAcademic Institutions and Nonprofits only -
pCHKU28.1-2.2
Plasmid#111049PurposeExpression of a fungal biosynthetic gene clusterDepositorInsertPKS
UseTagsExpressionYeastMutationPromoterAvailable sinceJune 11, 2018AvailabilityAcademic Institutions and Nonprofits only -
pcDNA3-EngPDm
Plasmid#64040PurposeMammalian expression of mutant paired domain of Pax8 fused to the N-terminal repressor domain of the drosophila engrailed protein (Eng)DepositorInsertPax8 paired domain (Pax8 Mouse)
UseTagsEng (N-terminal repressor domain of the drosophi…ExpressionMammalianMutationpaired domain, aa 1-147, with aa 30 to 32 changed…PromoterCMVAvailable sinceApril 23, 2015AvailabilityAcademic Institutions and Nonprofits only -
pcDNA3-EngHDm
Plasmid#64038PurposeMammalian expression of TTF-1 mutant homeodomain fused to the N-terminal repressor domain of the drosophila engrailed protein (Eng)DepositorInsertTTF-1 (TTF1 dog)
UseTagsEng (N-terminal repressor domain of the drosophi…ExpressionMammalianMutationhomeodomain, aa 154-226, with aa 48–50 replaced b…PromoterCMVAvailable sinceApril 23, 2015AvailabilityAcademic Institutions and Nonprofits only -
pBabe_puro_DEST_Flag_LRRC31
Plasmid#45271DepositorInsertLRRC31 (LRRC31 Human)
UseRetroviralTagsFlagExpressionMammalianMutationPromoterAvailable sinceMay 22, 2013AvailabilityAcademic Institutions and Nonprofits only -
alpha 0-CFP in pdest12.2
Plasmid#36312DepositorInsertG protein alpha 0 (Gnao1 Rat)
UseTagsCFPExpressionMammalianMutationIntroduced KpnI and SpeI sites between 92nd and 9…PromoterCMVAvailable sinceFeb. 14, 2013AvailabilityAcademic Institutions and Nonprofits only -
pAAV-TRE-DIO-hM4Di-mCherry
Plasmid#182532PurposeThis plasmid is for use with neuronal cell-type selective activity tagging. The hM4Di receptor expression requires both neural activity and Cre recombinase.DepositorInsertAAV transgene - hSyn promoter-tet-flex-hM4Di-cherry
UseAAV, Cre/Lox, and Mouse TargetingTagshM4Di-mCherryExpressionMammalianMutationPromotertetO promoterAvailable sinceMay 13, 2022AvailabilityAcademic Institutions and Nonprofits only -
RBM14_pLX307
Plasmid#98367PurposeLentiviral expression of RBM14DepositorInsertRBM14 (RBM14 Human)
UseLentiviralTagsV5ExpressionMammalianMutationPromoterE1FaAvailable sinceAug. 23, 2017AvailabilityAcademic Institutions and Nonprofits only