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HSPA8_pLX307

Plasmid

#98343

Purpose

Lentiviral expression of HSPA8

Depositor

William Hahn, Sefi Rosenbluh

Article

Rosenbluh et al Cell Syst. 2016 Sep 28;3(3):302-316.e4.

Insert

HSPA8 (HSPA8 Human)

Use

Lentiviral

Tags

V5

Expression

Mammalian

Promoter

E1Fa

Available Since

Aug. 23, 2017

Availability

Academic Institutions and Nonprofits only

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-962 human cyclin D1 promoter AP-1 site mutant pGL3Basic

Plasmid

#32728

Depositor

Frank McCormick

Article

Tetsu et al Nature. 1999 Apr 1;398(6726):422-6.

Insert

CCND1 promoter (CCND1 Human)

Use

Luciferase; Luciferase reporter gene

Mutation

-947 AP-1 binding site TGAGTCA deletion

Promoter

-962 CCND1 promoter

Available Since

Jan. 27, 2012

Availability

Academic Institutions and Nonprofits only
pAAV-EF1a-iDreV

Plasmid

#140136

Purpose

can be used to generate AAV virus that will express light-inducible site-specific iDreV recombinase

Depositor

Hongkui Zeng

Article

Yao et al Nat Methods. 2020 Mar 23. pii: 10.1038/s

Has Service

AAV PHP.eB and AAV1

Insert

iDreV

Use

AAV

Promoter

EF1a

Available Since

April 23, 2020

Availability

Academic Institutions and Nonprofits only

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LentiCRISPR v2-sgLKB1 clone1

Plasmid

#162125

Purpose

Lentiviral sgRNA plasmid targeting human LKB1

Depositor

Boyi Gan

Article

Lee et al Nat Cell Biol. 2020 Feb;22(2):225-234. d

Insert

sgLKB1 (STK11 Human)

Use

Lentiviral

Available Since

Jan. 26, 2021

Availability

Academic Institutions and Nonprofits only

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shGFP neo

Plasmid

#72571

Purpose

Hairpin targeting GFP.

Depositor

Kevin Janes

Article

Jensen et al Cell Syst. 2016 Feb 24;2(2):112-121.

Insert

GFP

Use

Lentiviral

Expression

Mammalian

Available Since

July 12, 2016

Availability

Academic Institutions and Nonprofits only

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RANLS-DEVD-BNES

Plasmid

#50840

Purpose

Expresses a tandem ddFP heterodimer in mammalian cells, plasmid 1 for a translocation-based red fluorescent caspase-3 biosensor, used together with plasmid 2 (BNLS).

Depositor

Robert Campbell

Article

Ding et al Nat Methods. 2015 Jan 26. doi: 10.1038/n

Insert

ddRFP A and ddRFP B

Tags

NES sequence LALKLAGLDIGS and triplicated NLS seq…

Expression

Mammalian

Promoter

CMV

Available Since

June 19, 2015

Availability

Academic Institutions and Nonprofits only

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Cav2.2e[a10,Δ18a, Δ24a, 31a, 37b, 46]

Plasmid

#26567

Depositor

Diane Lipscombe

Article

Lin et al Neuron. 1997 Jan . 18(1):153-66.

Insert

calcium channel alpha-1B subunit (Cacna1b Rat)

Tags

6XHis and V5

Expression

Mammalian

Mutation

Contains exons a10, 31a, 37b, and 46. Deletion…

Available Since

Nov. 29, 2010

Availability

Academic Institutions and Nonprofits only
p255 pPr-CREM/Ins/CMV-STOP-luc

Plasmid

#8408

Purpose

Modified cre expressed from probasin, separated from the Cre-dependent CMV-STOP-luc cassette by insulators.

Depositor

Jeffrey Green

Article

Kaczmarczyk et al Nucleic Acids Res 2001 Jun 15;29(12):E56

Insert

CREM/insulators/lox/luciferase

Use

Cre/Lox

Expression

Mammalian

Mutation

modified Cre

Promoter

Probasin, CMV

Available Since

April 26, 2006

Availability

Academic Institutions and Nonprofits only
pDONR221_SLC22A2

Plasmid

#131964

Purpose

Gateway entry clone with codon-optimized ORF sequence for subcloning into custom expression vectors

Depositor

RESOLUTE Consortium, Giulio Superti-Furga

Article

Mocking et al Int J Mol Sci. 2022 Jan 21;23(3):1203. d

Insert

SLC22A2 (SLC22A2 Human)

Expression

Mammalian

Available Since

Oct. 21, 2019

Availability

Industry, Academic Institutions, and Nonprofits

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pEM705 Gsq

Plasmid

#109356

Purpose

GsX chimera with Gαq C-terminus

Depositor

Robert Lucas

Article

Ballister et al BMC Biol. 2018 Jan 16;16(1):10. doi: 10.

Insert

Gsq chimera

Tags

None

Expression

Mammalian

Promoter

CAG

Available Since

May 11, 2018

Availability

Academic Institutions and Nonprofits only

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pMH4

Plasmid

#52529

Purpose

serves as PCR-template to generate HR donors for C-terminal 2xFLAG-tag

Depositor

Klaus Foerstemann

Article

Bottcher et al Nucleic Acids Res. 2014 Apr 19.

Insert

2x FLAG-tag

Use

Blunt cloning kit

Available Since

April 14, 2014

Availability

Academic Institutions and Nonprofits only

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pcDNA3-Flag-YOD1

Plasmid

#49784

Purpose

Express YOD1 with a N-terminal Flag-tag in mammalian cells

Depositor

Christian Schlieker

Article

Ernst et al Mol Cell. 2009 Oct 9;36(1):28-38. doi: 1

Insert

YOD1 (YOD1 Human)

Tags

Flag-tag

Mutation

WT

Available Since

Dec. 16, 2013

Availability

Academic Institutions and Nonprofits only
pHA-4EHP

Plasmid

#17344

Depositor

Dong-Er Zhang

Article

Okumura et al Genes Dev. 2007 Feb 1. 21(3):255-60.

Insert

4EHP (EIF4E2 Human)

Tags

HA

Expression

Mammalian

Available Since

Feb. 19, 2008

Availability

Academic Institutions and Nonprofits only
CMVdel-dimericTomato-WASp(CRIB)

Plasmid

#191450

Purpose

Visualizing endogenous & active Cdc42

Depositor

Dorus Gadella

Article

Mahlandt et al J Cell Sci. 2023 May 15;136(10):jcs26080

Insert

WASp

Expression

Mammalian

Available Since

Nov. 16, 2022

Availability

Academic Institutions and Nonprofits only

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MSCV-BCL2

Plasmid

#163611

Purpose

Overexpression of human BCL2

Depositor

Daniel Hodson

Article

Caeser et al Nat Commun. 2019 Oct 4;10(1):4543. doi:

Insert

BCL2 (BCL2 Human)

Use

Retroviral

Expression

Mammalian

Promoter

LTR

Available Since

Feb. 10, 2021

Availability

Academic Institutions and Nonprofits only

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pKSCTMF

Plasmid

#85073

Purpose

Gene insertion/deletion in Pichia pastoris

Depositor

Sheng Yang

Article

Yang et al FEMS Yeast Res. 2009 Jun;9(4):600-9. doi

Insert

AOX1promoter - mazF - AOX1 tt- Zeocin R gene

Use

Gene insertion/deletion in pichia pastoris

Promoter

pAOX1

Available Since

March 17, 2017

Availability

Academic Institutions and Nonprofits only

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pcDNA3-BaLgp120-8his

Plasmid

#100925

Purpose

Mammalian expression plasmid for gp120 subunit from the BaL HIV-1 strain

Depositor

Erik Procko

Article

Heredia et al J Virol. 2019 Mar 20. pii: JVI.00219-19.

Insert

HIV-1 (BaL) Env

Tags

CD5 leader sequence and Purification tag after K5…

Expression

Mammalian

Mutation

Codon-optimized synthetic gene

Promoter

CMV

Available Since

March 25, 2019

Availability

Academic Institutions and Nonprofits only

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shRNA Caspase 1 (pSicoR mCherry)

Plasmid

#53575

Purpose

shRNA against human pro-caspase 1

Depositor

Warner Greene

Article

Doitsh et al Nature. 2014 Jan 23;505(7484):509-14. do

Insert

shRNA Caspase 1 (CASP1 Human)

Use

Lentiviral and RNAi

Expression

Mammalian

Promoter

U6 for shRNA; EF1a for mCherry

Available Since

Aug. 7, 2015

Availability

Academic Institutions and Nonprofits only
pmiRA1-MYB

Plasmid

#141120

Purpose

Lentiviral overexpression of MYB in mammalian cells.

Depositor

Jianjun Chen

Article

Weng et al Cell Stem Cell. 2018 Feb 1;22(2):191-205

Insert

MYB (MYB Human)

Use

Lentiviral

Expression

Mammalian

Available Since

Dec. 14, 2020

Availability

Academic Institutions and Nonprofits only

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pCAG-Chimeric_Butterfly_CY_1.0

Plasmid

#59800

Purpose

Genetically encoded fluorescent voltage indicator

Depositor

Thomas Knopfel

Article

Mishina et al Front Mol Neurosci. 2014 Sep 29;7:78. do

Insert

Cyan-Yellow Chimeric Butterfly Voltage Sensitive Fluorescent Protein

Expression

Mammalian

Promoter

CAG

Available Since

Oct. 29, 2014

Availability

Academic Institutions and Nonprofits only

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