We narrowed to 64,855 results for: cel

1. pCS2+ 3xFLAG-FEM1B L597A

   Plasmid

   #230994

   Purpose

   Transient overexpression of catalytic null mouse FEM1B in mammalian cells. Mutation abolishes interaction with CUL2.

   Depositor

   Michael Rape

   Article

   Manford et al Cell. 2020 Oct 1;183(1):46-61.e21. doi:

   Insert

   FEM1B (Fem1b Mouse)
   

   Use

   Tags

   3xFLAG

   Expression

   Mammalian

   Mutation

   mutated Leu597 to Ala

   Promoter

   Available since

   Jan. 23, 2025

   Availability

   Academic Institutions and Nonprofits only

   

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2. pCAG-RhoA*

   Plasmid

   #198716

   Purpose

   Expresses gRNA resistant RhoA under the CAG promoter in mammalian cells

   Depositor

   Takeshi Imai

   Article

   Fujimoto et al Dev Cell. 2023 Jul 24;58(14):1221-1236.e

   Insert

   RhoA (Rhoa Mouse)
   

   Use

   Tags

   Expression

   Mammalian

   Mutation

   It has mutations that is resistant to the RhoA gR…

   Promoter

   CAG

   Available since

   Dec. 11, 2024

   Availability

   Academic Institutions and Nonprofits only

   

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3. pHMTc-FBF-2 (121-632)

   Plasmid

   #218664

   Purpose

   Expression of MBP:6xHIS:FBF-2(121-632)

   Depositor

   Judith Kimble

   Article

   Carrick et al Dev Cell. 2024 Mar 11;59(5):661-675.e7.

   Insert

   fbf-2 (fbf-2 Nematode)
   

   Use

   Tags

   MBP-6xHIS

   Expression

   Bacterial

   Mutation

   amino acids 121-632 only

   Promoter

   Available since

   July 24, 2024

   Availability

   Academic Institutions and Nonprofits only

   

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4. pJK2097

   Plasmid

   #218665

   Purpose

   Expression of MBP:6xHIS:FBF-2(Y479A, 121-632)

   Depositor

   Judith Kimble

   Article

   Carrick et al Dev Cell. 2024 Mar 11;59(5):661-675.e7.

   Insert

   fbf-2 (fbf-2 Nematode)
   

   Use

   Tags

   MBP-6xHIS

   Expression

   Bacterial

   Mutation

   amino acids 121-632 only, with Y479A mutation

   Promoter

   Available since

   May 28, 2024

   Availability

   Academic Institutions and Nonprofits only

   

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5. pcS-RDG-C1C2

   Plasmid

   #200163

   Purpose

   This plasmid encodes non-binder control monobody (RDG) fused to the extracellular vesicle binding domain (C1C2) of lactadherin for surface engineering extracellular vesicles.

   Depositor

   Masako Harada

   Article

   Komuro et al Bioengineering (Basel). 2022 Jan 29;9(2)

   Inserts

   RDG-C1C2
   C1C2 domain of lactadherin
   

   Use

   Tags

   HA and HIS

   Expression

   Mammalian

   Mutation

   sequence adopted from doi: 10.1371/journal.pone.0…

   Promoter

   Available since

   Sept. 29, 2023

   Availability

   Academic Institutions and Nonprofits only

   

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6. phABCA3-eGFP_CRISPR_Donor

   Plasmid

   #188540

   Purpose

   Homologous recombination donor plasmid for CRISPR/Cas9 targeting of GFP fusion protein to the stop codon of endogenous human ABCA3 gene locus

   Depositor

   Darrell Kotton

   Article

   Sun et al Am J Respir Cell Mol Biol. 2021 Oct;65(4

   Insert

   EGFP
   

   Use

   CRISPR; Donor for homologous recombination

   Tags

   Expression

   Mutation

   Promoter

   Available since

   Sept. 9, 2022

   Availability

   Academic Institutions and Nonprofits only

   

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7. pCDNA4TO-3XFLAG Halo R99A nsp1

   Plasmid

   #175422

   Purpose

   Expresses R99A CoV2 nsp1 in mammalian cells. This contains a 3XFLAG Halo tag fusion to nsp1.

   Depositor

   Britt Glaunsinger

   Article

   Mendez et al Cell Rep. 2021 Oct 19;37(3):109841. doi:

   Insert

   CoV2 R99A nsp1
   

   Use

   Tags

   3XFLAG Halo

   Expression

   Mammalian

   Mutation

   Promoter

   CMV

   Available since

   Oct. 8, 2021

   Availability

   Academic Institutions and Nonprofits only

   

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8. LR (hsp70I: cPLA2-mK2)

   Plasmid

   #162564

   Purpose

   Measuring cPLA2 activation in live cells after heat shock.

   Depositor

   Philipp Niethammer

   Article

   Enyedi et al Cell. 2016 May 19;165(5):1160-1170. doi:

   Inserts

   Heat shock protein 70 (HSPA1A Human)
   cytosolic phospholipase a2 (pla2g4aa Zebrafish)
   

   Use

   Gateway donor vector

   Tags

   mK2

   Expression

   Mutation

   Promoter

   cmlc2

   Available since

   March 2, 2021

   Availability

   Academic Institutions and Nonprofits only

   

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9. Clim Actin MCH Puro (CAMP)

   Plasmid

   #104446

   Purpose

   Expresses mCherry under endogenous actin promoter. It can be used to transfect Corallochytrium limacisporum (Corallochytrea) cells and visualize them by fluorescent microscope. Puromycin resistant.

   Depositor

   Inaki Ruiz-Trillo, Elena Casacuberta

   Article

   Kozyczkowska et al Curr Biol. 2021 Sep 27;31(18):4104-4110.

   Insert

   mCherry
   

   Use

   Expression in corallochytrium (single-celled euka…

   Tags

   Expression

   Mutation

   Promoter

   actin

   Available since

   Feb. 16, 2021

   Availability

   Academic Institutions and Nonprofits only

   

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10. pSicoR (EGFP) shPnky-2

    Plasmid

    #79142

    Purpose

    Stable expression of shRNA targeting mouse Pnky. The shRNA (and EGFP) can be excised by the addition of Cre

    Depositor

    Daniel Lim

    Article

    Ramos et al Cell Stem Cell. 2015 Apr 2;16(4):439-47.

    Insert

    shPnky-2 (Gm30731 Mouse)
    

    Use

    Cre/Lox, Lentiviral, and RNAi

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    U6 for the shRNA and CMV for EGFP

    Available since

    June 29, 2016

    Availability

    Academic Institutions and Nonprofits only

    

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11. pScalps_puro_mIL-2Rα ST

    Plasmid

    #59919

    Purpose

    Expresses mouse interleukin-2 receptor alpha chain mutated

    Depositor

    Silvia Monticelli

    Article

    Deho' et al J Immunol. 2014 Sep 1;193(5):2196-206. d

    Insert

    interleukin 2 receptor alpha (Il2ra Mouse)
    

    Use

    Lentiviral

    Tags

    Expression

    Mutation

    changed sequence of the intracellular (C-terminal…

    Promoter

    Available since

    Oct. 3, 2014

    Availability

    Academic Institutions and Nonprofits only

12. pGEX4T-1 hSlp4-a I18A

    Plasmid

    #40066

    Depositor

    Fernando Martin-Belmonte

    Article

    Galvez-Santisteban et al Nat Cell Biol. 2012 Jul 22. doi: 10.1038

    Insert

    hSlp4-a I18A (SYTL4 Human)
    

    Use

    Tags

    GST

    Expression

    Bacterial

    Mutation

    Isoleucine 18 to Alanine

    Promoter

    Tac

    Available since

    Oct. 16, 2012

    Availability

    Academic Institutions and Nonprofits only

13. pGEX4T-1 hSlp4-a W118S

    Plasmid

    #40065

    Depositor

    Fernando Martin-Belmonte

    Article

    Galvez-Santisteban et al Nat Cell Biol. 2012 Jul 22. doi: 10.1038

    Insert

    hSlp4-a W118S (SYTL4 Human)
    

    Use

    Tags

    GST

    Expression

    Bacterial

    Mutation

    Tryptophane 118 to Serine

    Promoter

    Tac

    Available since

    Oct. 16, 2012

    Availability

    Academic Institutions and Nonprofits only

14. pEB-C5

    Plasmid

    #28213

    Purpose

    5 reprogramming factors (OCT4, SOX2, KLF4, c-MYC and LIN28) are expressed as a single poly-cistronic unit

    Depositor

    Linzhao Cheng

    Article

    Chou et al Cell Res. 2011 Mar;21(3):518-29. doi: 10

    Insert

    Oct4, Sox2, Klf4, c-Myc, Lin28 (Myc, Sox2, Pou5f1, Klf4, Lin28a Mouse)
    

    Use

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    Available since

    June 20, 2011

    Availability

    Academic Institutions and Nonprofits only

    

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15. pcDNA-Ikkb-FLAG WT

    Plasmid

    #23298

    Depositor

    Warner Greene

    Article

    Geleziunas et al Mol Cell Biol. 1998 Sep . 18(9):5157-65.

    Insert

    Ikkbeta (IKBKB Human)
    

    Use

    Tags

    Flag

    Expression

    Mammalian

    Mutation

    Promoter

    Available since

    March 24, 2010

    Availability

    Academic Institutions and Nonprofits only

    

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16. pcDNA3 2xFLAG-2xSTREP E2F4

    Plasmid

    #236439

    Purpose

    transient overexpression of E2F4 in mammalian cells

    Depositor

    Michele Pagano

    Article

    Clijsters et al Mol Cell. 2019 Jun 20;74(6):1264-1277.e7

    Insert

    E2F4 (E2F4 Human)
    

    Use

    Tags

    FLAG-FLAG-STREP-STREP

    Expression

    Mammalian

    Mutation

    Promoter

    Available since

    May 20, 2025

    Availability

    Academic Institutions and Nonprofits only

    

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17. Lenti-Cre

    Plasmid

    #198712

    Purpose

    For lentiviral expression of Cre recombinase in mammalian cells.

    Depositor

    Tyler Jacks

    Article

    Gaglia et al Cancer Cell. 2023 May 8;41(5):871-886.e1

    Insert

    Pgk-Cre
    

    Use

    Cre/Lox and Lentiviral

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    PGK

    Available since

    Jan. 7, 2025

    Availability

    Academic Institutions and Nonprofits only

    

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18. pcDNA3-HA-RAPGEF2

    Plasmid

    #110162

    Purpose

    Expression of human RAPGEF2 in mammalian cells

    Depositor

    Daniele Guardavaccaro

    Article

    Magliozzi et al Dev Cell. 2013 Dec 9;27(5):574-85. doi:

    Insert

    RAPGEF2 (Rap guanine nucleotide exchange factor 2 (RAPGEF2 Human)
    

    Use

    Tags

    HA

    Expression

    Mammalian

    Mutation

    Promoter

    CMV

    Available since

    May 18, 2018

    Availability

    Academic Institutions and Nonprofits only

    

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19. AAVS1-TRE3G-NFIA+SOX9

    Plasmid

    #129455

    Purpose

    Inducible overexpression of NFIA and Sox9 in human cells.

    Depositor

    Su-Chun Zhang

    Article

    Li et al Stem Cell Reports. 2018 Oct 9;11(4):998-

    Insert

    Sox 9 (SOX9 Human)
    

    Use

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    TRE3G

    Available since

    March 5, 2020

    Availability

    Academic Institutions and Nonprofits only

    

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20. pcDNA3 2xFLAG-2xSTREP E2F7

    Plasmid

    #236442

    Purpose

    transient overexpression of E2F7 in mammalian cells

    Depositor

    Michele Pagano

    Article

    Clijsters et al Mol Cell. 2019 Jun 20;74(6):1264-1277.e7

    Insert

    E2F7 (E2F7 Human)
    

    Use

    Tags

    FLAG-FLAG-STREP-STREP

    Expression

    Mammalian

    Mutation

    Promoter

    Available since

    May 20, 2025

    Availability

    Academic Institutions and Nonprofits only

    

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