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pCS2-nCas9n-nanos 3’UTR

Plasmid

#62542

Purpose

expression of a germ-line targeted, codon optimized Cas9 for genome-editing in zebrafish

Depositor

Antonio Giraldez

Article

Moreno-Mateos et al Nat Methods. 2015 Aug 31. doi: 10.1038/n

Insert

nanos 3' UTR (nanos3 Zebrafish)

Use

CRISPR

Tags

Expression

Mutation

3' UTR only

Promoter

SP6

Available since

July 28, 2015

Availability

Academic Institutions and Nonprofits only

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pEGFP-sfCherry11x4-ß-actin

Plasmid

#70220

Purpose

Expresses sfCherry11x4-ß-actin in mammalian cells

Depositor

Bo Huang

Article

Kamiyama et al Nat Commun. 2016 Mar 18;7:11046. doi: 10

Insert

sfCherry11x4-actin (ACTB Human)

Use

Tags

sfCherry11x4

Expression

Mammalian

Mutation

Promoter

Available since

March 21, 2016

Availability

Academic Institutions and Nonprofits only

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nuclear EKAR (Cerulean-Venus)

Plasmid

#18681

Depositor

Karel Svoboda

Article

Harvey et al PNAS 2008 Dec 9;105(49):19264-9

Insert

ERK Activity Reporter

Use

Tags

monomeric Cerulean and monomeric Venus

Expression

Mammalian

Mutation

Promoter

Available since

July 2, 2008

Availability

Academic Institutions and Nonprofits only

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pCS2-NXE+mem-KillerRed

Plasmid

#45761

Depositor

Michael Levin

Insert

Membrane-bound KillerRed

Use

Xenopus expression

Tags

Expression

Mutation

Promoter

CMV IE94

Available since

July 9, 2013

Availability

Academic Institutions and Nonprofits only

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APEX2-GBP

Plasmid

#67651

Purpose

Mammalian expression vector driving APEX2-GBP (EM peroxidase marker fused to GFP-targeting nanobody).

Depositor

Rob Parton

Article

Ariotti et al Dev Cell. 2015 Nov 23;35(4):513-25. doi:

Insert

APEX2-GBP

Use

Tags

Expression

Mammalian

Mutation

Promoter

CMV

Available since

Nov. 30, 2015

Availability

Academic Institutions and Nonprofits only

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pWPXLd

Plasmid

#12258

Depositor

Didier Trono

Type

Empty backbone

Use

Lentiviral

Tags

EGFP

Expression

Mammalian

Mutation

Promoter

Available since

Aug. 4, 2006

Availability

Academic Institutions and Nonprofits only

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CTV

Plasmid

#15912

Depositor

Klaus Rajewsky

Article

Thai et al Science. 2007 Apr 27. 316(5824):604-8.

Insert

CAG-STOP-eGFP-ROSA26TV

Use

Cre/Lox; /stop

Tags

Expression

Mutation

Promoter

Available since

Oct. 26, 2007

Availability

Academic Institutions and Nonprofits only

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pMXs-gw

Plasmid

#18656

Depositor

Shinya Yamanaka

Article

Takahashi et al Cell. 2006 Aug 25. 126(4):663-76.

Insert

Gateway cassette A

Use

Retroviral

Tags

Expression

Mammalian

Mutation

Promoter

Available since

Feb. 26, 2010

Availability

Academic Institutions and Nonprofits only

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LRP6-pCS2

Plasmid

#27242

Depositor

Xi He

Article

Tamai et al Nature. 2000 Sep 28. 407(6803):530-5.

Insert

low density lipoprotein receptor-related protein 6 (LRP6 Human)

Use

Xenopus injection

Tags

Expression

Mammalian

Mutation

Promoter

Available since

Feb. 10, 2011

Availability

Academic Institutions and Nonprofits only

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pCFD6

Plasmid

#73915

Purpose

tissue-specific multiplex gRNA plasmid

Depositor

Simon Bullock

Article

Port et al Nat Methods. 2016 Oct;13(10):852-4. doi:

Insert

:tRNA:gRNA:tRNA:gRNA:tRNA

Use

CRISPR

Tags

Expression

Insect

Mutation

Promoter

UAS-DSCP

Available since

Aug. 29, 2016

Availability

Academic Institutions and Nonprofits only

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pCDNA3 Flag Jnk1a1(apf)

Plasmid

#13846

Depositor

Roger Davis

Article

Derijard et al Cell. 1994 Mar 25. 76(6):1025-37.

Insert

Jnk1a1(apf) (MAPK8 Human)

Use

Tags

Flag

Expression

Mammalian

Mutation

replaced dual phosporylation motif Thr(183)-Pro-T…

Promoter

Available since

Feb. 22, 2007

Availability

Academic Institutions and Nonprofits only

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pYPQ143

Plasmid

#69295

Purpose

Golden Gate recipient and Gateway entry vector; assembly of 3 gRNAs

Depositor

Yiping Qi

Article

Lowder et al Plant Physiol. 2015 Aug 21. pii: pp.0063

Type

Empty backbone

Use

CRISPR

Tags

Expression

Mutation

Promoter

Available since

Nov. 4, 2015

Availability

Academic Institutions and Nonprofits only

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pFR_CrPV_xb

Plasmid

#11509

Depositor

Phil Sharp

Article

Petersen et al Mol Cell. 2006 Feb 17. 21(4):533-42.

Insert

Firefly luciferase, Cricket Paralysis Virus IRES sequence, Renilla, and 3'UTR short RNA binding sites

Use

Luciferase

Tags

Expression

Mutation

Promoter

Available since

March 15, 2006

Availability

Academic Institutions and Nonprofits only

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Flag-HA-USP18

Plasmid

#22572

Depositor

Wade Harper

Article

Sowa et al Cell. 2009 Jul 23. 138(2):389-403.

Insert

USP18 (USP18 Human)

Use

Retroviral

Tags

FLAG and HA

Expression

Mammalian

Mutation

None

Promoter

Available since

Nov. 13, 2009

Availability

Academic Institutions and Nonprofits only

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GFP-HP1beta

Plasmid

#17651

Depositor

Tom Misteli

Article

Cheutin et al Science. 2003 Jan 31. 299(5607):721-5.

Insert

HP1 beta (CBX1 Human)

Use

Tags

GFP and His

Expression

Mammalian

Mutation

Promoter

Available since

March 27, 2008

Availability

Academic Institutions and Nonprofits only

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pH2B_mCherry_IRES_puro2

Plasmid

#21045

Depositor

Daniel Gerlich

Article

Steigemann et al Cell. 2009 Feb 6. 136(3):473-84.

Insert

pH2B (H2BC16P Human)

Use

Tags

mCherry

Expression

Bacterial and Mammalian

Mutation

Promoter

Available since

May 13, 2009

Availability

Academic Institutions and Nonprofits only

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HA-HIF1alpha P402A/P564A-pBabe-puro

Plasmid

#19005

Depositor

William Kaelin

Article

Yan et al Mol Cell Biol. 2007 Mar . 27(6):2092-102

Insert

Hypoxia inducible factor 1 alpha (HIF1A Human)

Use

Retroviral

Tags

HA-tag

Expression

Mammalian

Mutation

cDNA changed amino acids: Proline 402 to Alanin…

Promoter

Available since

Aug. 20, 2008

Availability

Academic Institutions and Nonprofits only

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PT3.5/CMV-EGFRvIII

Plasmid

#20280

Depositor

John Ohlfest

Article

Wiesner et al Cancer Res. 2009 Jan 15. 69(2):431-9.

Insert

EGFRvII (EGFR Human)

Use

Transposon

Tags

Expression

Mutation

See depositor comments below.

Promoter

Available since

Dec. 3, 2009

Availability

Academic Institutions and Nonprofits only

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pBabe EGFR(L858R/T790M)

Plasmid

#32073

Depositor

Matthew Meyerson

Article

Yuza et al Cancer Biol Ther. 2007 May . 6(5):661-7.

Insert

EGFR (EGFR Human)

Use

Retroviral

Tags

Expression

Mammalian

Mutation

L858R/T790M

Promoter

Available since

Sept. 19, 2011

Availability

Academic Institutions and Nonprofits only

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pAAV CD68-hM4D(Gi)-mCherry

Plasmid

#75033

Purpose

Expression of hM4D(Gi) using the CD68 promoter.

Depositor

Bryan Roth

Article

Grace et al Proc Natl Acad Sci U S A. 2016 Jun 14;11

Has Service

AAV1 and AAV9

Insert

hM4D(Gi)-mCherry

Use

AAV

Tags

Expression

Mammalian

Mutation

Promoter

CD68

Available since

Oct. 6, 2016

Availability

Academic Institutions and Nonprofits only

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