4,139 results

1. FLHKI-pGFPN3

   Plasmid

   #21917

   Depositor

   Hossein Ardehali

   Article

   Sun et al Mol Cell Biol. 2008 Feb . 28(3):1007-17.

   Insert

   Full length human HKI in pGFP-N3 (HK1 Human)
   

   Use

   Tags

   GFP

   Expression

   Mammalian

   Mutation

   This is full length human HKI in pGFP-N3

   Promoter

   Available since

   Sept. 17, 2009

   Availability

   Academic Institutions and Nonprofits only

   

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2. pCS2 mt-GFP (BamHI minus)

   Plasmid

   #15681

   Depositor

   Michael Klymkowsky

   Type

   Empty backbone

   Use

   Xenopus/mammalian/avian/zebrafish

   Tags

   6x Myc and GFP

   Expression

   Mutation

   Promoter

   Available since

   Sept. 14, 2007

   Availability

   Academic Institutions and Nonprofits only

   

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3. pU6-sgGFP-NT1

   Plasmid

   #46914

   Purpose

   Human pSico-based U6 vector containing murine U6 promoter and sgRNA targeting GFP (NT1)

   Depositor

   Jonathan Weissman, Stanley Qi

   Article

   Gilbert et al Cell. 2013 Jul 9. pii: S0092-8674(13)008

   Insert

   sgGFP-NT1
   

   Use

   CRISPR and Lentiviral

   Tags

   Expression

   Mammalian

   Mutation

   Promoter

   U6

   Available since

   Oct. 1, 2013

   Availability

   Academic Institutions and Nonprofits only

   

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4. HA GSK3 beta K85A pcDNA3

   Plasmid

   #14755

   Depositor

   Jim Woodgett

   Article

   Hughes et al EMBO J. 1993 Feb . 12(2):803-8.

   Insert

   GSK3 beta K85A (GSK3B Human)
   

   Use

   Tags

   HA

   Expression

   Mammalian

   Mutation

   K85A. Kinase dead mutant.

   Promoter

   Available since

   April 12, 2007

   Availability

   Industry, Academic Institutions, and Nonprofits

   

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5. p414-TEF1p-Cas9-CYC1t

   Plasmid

   #43802

   Purpose

   A human codon-optimized Cas9 for expression in S. cerevisiae (budding yeast) from the TEF1 promoter

   Depositor

   George Church

   Article

   Dicarlo et al Nucleic Acids Res

   Insert

   Human Optimized S. pyogenes Cas9
   

   Use

   CRISPR

   Tags

   Expression

   Yeast

   Mutation

   Promoter

   TEF1 promoter

   Available since

   March 15, 2013

   Availability

   Academic Institutions and Nonprofits only

   

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6. pBAD/His-miRFP670

   Plasmid

   #79984

   Purpose

   Monomeric near-infrared fluorescent protein miRFP670 in bacterial plasmid

   Depositor

   Vladislav Verkhusha

   Article

   Shcherbakova et al Nat Commun. 2016 Aug 19;7:12405. doi: 10

   Insert

   miRFP670
   

   Use

   Tags

   Expression

   Bacterial

   Mutation

   Promoter

   araB

   Available since

   July 20, 2016

   Availability

   Academic Institutions and Nonprofits only

   

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7. pET15b PKA Cat

   Plasmid

   #14921

   Depositor

   Susan Taylor

   Article

   Narayana et al Biochemistry. 1997 Apr 15. 36(15):4438-4

   Insert

   PKA catalytic subunit alpha (Prkaca Mouse)
   

   Use

   Tags

   6xHis and Thrombin cleavage site

   Expression

   Bacterial

   Mutation

   Promoter

   Available since

   July 24, 2007

   Availability

   Academic Institutions and Nonprofits only

   

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8. pDONR207 SARS-CoV-2 NSP3

   Plasmid

   #141257

   Purpose

   Gateway-compatible Entry vector, with insert of NSP3 gene's CDS from SARS-CoV-2 isolate Wuhan-Hu-1

   Depositor

   Fritz Roth

   Article

   Kim et al G3 (Bethesda). 2020 Aug 6. pii: g3.120.4

   Has Service

   Cloning Grade DNA

   Insert

   NSP3 (ORF1ab SARS-CoV-2 isolate Wuhan-Hu-1)
   

   Use

   Gateway-compatible entry vector

   Tags

   Expression

   Mutation

   Many synonymous changes due to codon optimization

   Promoter

   Available since

   April 1, 2020

   Availability

   Industry, Academic Institutions, and Nonprofits

   

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9. pLM-vexGFP-Oct4

   Plasmid

   #22240

   Purpose

   2nd generation lentiviral vector

   Depositor

   Michel Sadelain

   Article

   Papapetrou et al Proc Natl Acad Sci U S A. 2009 Aug 4. 10

   Insert

   vexGFP_2A_OCT4 (POU5F1 synthetic fluorescent protein, Human)
   

   Use

   Lentiviral

   Tags

   Expression

   Mutation

   Promoter

   Available since

   Feb. 5, 2010

   Availability

   Academic Institutions and Nonprofits only

   

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10. pRK5-Myc-Parkin

    Plasmid

    #17612

    Depositor

    Ted Dawson

    Article

    Zhang et al Proc Natl Acad Sci U S A 2000 Nov 21;97(

    Insert

    Parkin (PRKN Human)
    

    Use

    Tags

    Myc

    Expression

    Mammalian

    Mutation

    Promoter

    Available since

    March 31, 2008

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

11. 1245 pCMVb p300

    Plasmid

    #10717

    Depositor

    William Sellers

    Insert

    p300 (EP300 Human)
    

    Use

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    Available since

    Dec. 7, 2005

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

12. pAAV-hSyn-DIO-rM3D(Gs)-mCherry

    Plasmid

    #50458

    Purpose

    Double floxed Gs-coupled rM3D DREADD fused with mCherry under the control of human synapsin promoter

    Depositor

    Bryan Roth

    Has Service

    AAV8

    Insert

    rM3D-mCherry
    

    Use

    AAV

    Tags

    HA and mCherry

    Expression

    Mutation

    See supplemental documents for DREADD mutations

    Promoter

    human Synapsin 1

    Available since

    April 16, 2014

    Availability

    Academic Institutions and Nonprofits only

    

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13. human STIM1 YFP

    Plasmid

    #19754

    Depositor

    Anjana Rao

    Article

    Prakriya et al Nature. 2006 Sep 14. 443(7108):230-3.

    Insert

    STIM1 (STIM1 Human)
    

    Use

    Retroviral

    Tags

    YFP

    Expression

    Mammalian

    Mutation

    Promoter

    Available since

    Oct. 27, 2008

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

14. pcDNA3 HA 14-3-3 sigma

    Plasmid

    #11946

    Depositor

    Michael Yaffe

    Article

    Wilker et al J Biol Chem. 2005 May 13. 280(19):18891-

    Insert

    Stratifin (SFN Human)
    

    Use

    Tags

    HA

    Expression

    Mammalian

    Mutation

    Promoter

    Available since

    May 17, 2006

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

15. pQE-WT-RGVG-M6

    Plasmid

    #70172

    Purpose

    Expresses RGVG-M6 T7 RNA polymerase variant with enhanced transcription of 2' modified RNA

    Depositor

    Andrew Ellington

    Article

    Meyer et al Nucleic Acids Res. 2015 Sep 3;43(15):748

    Insert

    RGVG-M6 T7 RNA polymerase
    

    Use

    Tags

    His

    Expression

    Bacterial

    Mutation

    Promoter

    T5/Lac

    Available since

    Dec. 16, 2015

    Availability

    Industry, Academic Institutions, and Nonprofits

    

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16. pENTR4-V5 (w71-3)

    Plasmid

    #17425

    Purpose

    Gateway entry vector for N-term V5 tag, frame A

    Depositor

    Paul Kaufman, Eric Campeau

    Article

    Campeau et al PLoS One. 2009 Aug 6;4(8):e6529.

    Type

    Empty backbone

    Use

    Entry vector

    Tags

    V5

    Expression

    Mammalian

    Mutation

    Promoter

    Available since

    Aug. 12, 2009

    Availability

    Academic Institutions and Nonprofits only

    

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17. SuperNova/pRSETB

    Plasmid

    #53234

    Purpose

    monomeric photosensitizing fluorescent protein for chromophore-assisted light inactivation

    Depositor

    Takeharu Nagai

    Article

    Takemoto et al Sci Rep. 2013;3:2629. doi: 10.1038/srep0

    Insert

    monomeric photosensitizing fluorescent protein for chromophore-assisted light inactivation
    

    Use

    Tags

    6xHis, T7 tag, and Xpress tag

    Expression

    Bacterial

    Mutation

    Promoter

    Available since

    June 9, 2014

    Availability

    Academic Institutions and Nonprofits only

    

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18. pET9d-hnRNP-A1

    Plasmid

    #23026

    Depositor

    Douglas Black

    Insert

    hnRNPA1 (HNRNPA1 Human)
    

    Use

    Tags

    Expression

    Bacterial

    Mutation

    Promoter

    T7

    Available since

    Oct. 26, 2012

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

19. pcDNA3-FLAG-Rheb

    Plasmid

    #19996

    Depositor

    Fuyuhiko Tamanoi

    Article

    Urano et al Proc Natl Acad Sci U S A. 2007 Feb 27. 1

    Insert

    Ras homolog enriched in brain 1 (RHEB Human)
    

    Use

    Tags

    FLAG

    Expression

    Mammalian

    Mutation

    Promoter

    Available since

    Jan. 28, 2009

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

20. pBPGAL4.2::VP16Uw

    Plasmid

    #26228

    Depositor

    Gerald Rubin

    Article

    Pfeiffer et al Genetics. 2010 Oct;186(2):735-55. doi: 1

    Insert

    GAL4::VP16 (GAL4 Budding Yeast)
    

    Use

    Tags

    Expression

    Insect

    Mutation

    Promoter

    Available since

    Oct. 7, 2010

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge