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Anti-GIT2 [N83/48R]

Plasmid

#140079

Purpose

Mammalian Expression Plasmid of anti-GIT2 (Human). Derived from hybridoma N83/48.

Depositor

James Trimmer

Article

Andrews et al Elife. 2019 Jan 22;8. pii: 43322. doi: 1

Insert

anti-GIT2 (Human) recombinant mouse monoclonal antibody (GIT2 Mouse)

Use

Tags

Expression

Mammalian

Mutation

Promoter

dual CMV

Available since

April 24, 2020

Availability

Academic Institutions and Nonprofits only

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pCI-HA-mAVO3 (pRL62-3)

Plasmid

#39210

Depositor

Michael Hall

Article

Jacinto et al Nat Cell Biol. 2004 Nov;6(11):1122-8. Ep

Insert

mAVO3 (Rictor Mouse)

Use

Tags

HA

Expression

Mammalian

Mutation

G5S and I15V

Promoter

CMV

Available since

Sept. 4, 2012

Availability

Academic Institutions and Nonprofits only
PLEXIN A4

Plasmid

#58239

Purpose

Riboprobe for in vitro transcription of Plexin A4

Depositor

Christiana Ruhrberg

Article

Schwarz et al Dev Biol. 2008 Dec 1;324(1):1-9. doi: 10

Insert

Plexin A4 (Plxna4 Mouse)

Use

Tags

Expression

Bacterial

Mutation

Promoter

Available since

Aug. 29, 2014

Availability

Academic Institutions and Nonprofits only
pCAGGS-Trex2

Plasmid

#79246

Purpose

Expression vector for Trex2, which is a non-processive 3' exonuclease.

Depositor

Jeremy Stark

Article

Bennardo et al PLoS Genet. 2009 Oct;5(10):e1000683. doi

Insert

Trex2 (Trex2 Mouse)

Use

Tags

Expression

Mammalian

Mutation

Promoter

pCAGGS

Available since

Aug. 16, 2016

Availability

Academic Institutions and Nonprofits only

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MSCV FLIPi P2G_Thy1.1 PTEN

Plasmid

#19749

Depositor

Richard Hynes

Article

Stern et al Proc Natl Acad Sci U S A. 2008 Sep 16. 1

Insert

oligo targeting PTEN (Pten Mouse)

Use

Cre/Lox, RNAi, and Retroviral

Tags

Expression

Mammalian

Mutation

Promoter

Available since

Dec. 18, 2008

Availability

Academic Institutions and Nonprofits only

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FRB_T2098L-VAMP3-mCherry

Plasmid

#92429

Purpose

Expression of VAMP3 N-terminally conjugated to FRB and C-terminally conjugated to mCitrine.

Depositor

Geert van den Bogaart

Article

Verboogen et al Elife. 2017 May 19;6. pii: e23525. doi:

Insert

VAMP3 (Vamp3 Mouse)

Use

Tags

FKBP rapamycin binding (FRB) domain of mTOR with …

Expression

Mammalian

Mutation

Promoter

CMV

Available since

July 12, 2017

Availability

Academic Institutions and Nonprofits only

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pBABE puro mouse IRS-2

Plasmid

#11371

Depositor

Ronald Kahn

Article

Tsuruzoe et al Mol Cell Biol. 2001 Jan . 21(1):26-38.

Insert

IRS-2 (Irs2 Mouse)

Use

Retroviral

Tags

Expression

Mammalian

Mutation

Promoter

Available since

April 4, 2006

Availability

Academic Institutions and Nonprofits only
mEmerald-VASP-N-10

Plasmid

#54297

Purpose

Localization: Actin/Focal Adhesions, Excitation: 487, Emission: 509

Depositor

Michael Davidson

Insert

VASP

Use

Tags

mEmerald

Expression

Mammalian

Mutation

A209T in VASP

Promoter

CMV

Available since

June 16, 2014

Availability

Industry, Academic Institutions, and Nonprofits
PSD-93/Chapsyn-110 scFv [N18/28]

Plasmid

#190535

Purpose

Mammalian Expression of PSD-93/Chapsyn-110 scFV. Derived from hybridoma N18/28.

Depositor

James Trimmer

Article

Mitchell et al Sci Rep. 2023 Sep 27;13(1):16200. doi: 1

Insert

PSD-93/Chapsyn-110 (Rattus norvegicus) recombinant scFV (Dlg2 Mouse)

Use

Tags

HA, Sortase, 6xHis

Expression

Mammalian

Mutation

Promoter

CMV

Available since

Nov. 1, 2022

Availability

Academic Institutions and Nonprofits only

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pFRT-PonA-BI-Gl-WT-24xMS2-WT-18xPP7 (WW)

Plasmid

#189791

Purpose

Express wild-type beta-globin genes bi-directionally under ponasteron A inducible promoter in mammalian cells

Depositor

Robert Singer

Article

Sato et al Nat Commun. 2021 Dec 10;12(1):7203. doi:

Insert

beta-globin (HBB Mouse, Human)

Use

Tags

Expression

Mammalian

Mutation

Promoter

Ponasterone A inducible

Available since

March 9, 2023

Availability

Academic Institutions and Nonprofits only

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pET28b-Ets1-ETS

Plasmid

#85735

Purpose

Expresses DNA-binding (ETS) domain of murine Ets-1, residues 311 to 440

Depositor

Gregory Poon

Article

Wang et al J Biol Chem. 2014 Aug 1;289(31):21605-16

Insert

DNA-binding (ETS) domain of murine Ets-1, residues 331 to 440 (Ets1 Mouse, Human)

Use

Tags

6xHis following thrombin cleavage site LVPR|G

Expression

Bacterial

Mutation

Promoter

Available since

Feb. 24, 2017

Availability

Academic Institutions and Nonprofits only

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pEntr-Nkx2-5flbio

Plasmid

#32969

Depositor

William Pu

Article

He et al Proc Natl Acad Sci U S A. 2011 Apr 5. 10

Insert

Nkx2-5 (Nkx2-5 Mouse)

Use

Gateway

Tags

flag-bio

Expression

Mutation

Promoter

Available since

Jan. 9, 2012

Availability

Academic Institutions and Nonprofits only

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pFlox_N-RPB1_BSD_FKBPF36V_EYFP

Plasmid

#163884

Purpose

Acute Depletion of RBP1-EYFP

Depositor

Cornelis Murre

Article

Zhu et al Genes Dev. 2021 Jan 1;35(1-2):65-81. doi

Insert

Rbp1 (Polr2a Mouse)

Use

Tags

Expression

Mammalian

Mutation

Promoter

Available since

Feb. 19, 2021

Availability

Academic Institutions and Nonprofits only

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Anti-MFF [N382/14]

Plasmid

#190318

Purpose

Mammalian Expression Plasmid of anti-MFF (Human). Derived from hybridoma N382/14.

Depositor

James Trimmer

Article

Mitchell et al Sci Rep. 2023 Sep 27;13(1):16200. doi: 1

Insert

anti-MFF (Homo sapiens) recombinant Mouse monoclonal antibody (MFF Mouse)

Use

Tags

Expression

Mammalian

Mutation

Promoter

Dual CMV

Available since

Nov. 1, 2022

Availability

Academic Institutions and Nonprofits only

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pColdI-GST-mGTSF1l

Plasmid

#214817

Purpose

Bacterial expression of GST-tagged mouse GTSF1l

Depositor

Phillip Zamore

Article

Arif et al Nature. 2022 Aug;608(7923):618-625. doi:

Insert

GTSF1l (Gtsf1l Mouse)

Use

Tags

6x His and GST

Expression

Bacterial

Mutation

Promoter

CspA

Available since

March 20, 2024

Availability

Academic Institutions and Nonprofits only

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mCherry-CRY2–mPKCε-CAT-HA

Plasmid

#190483

Purpose

Expresses a fluorescently-tagged, optogenetically activated PKC-epsilon. Used in tandem with CIBN-CAAX

Depositor

Diomedes Logothetis

Article

Gada et al J Biol Chem. 2022 May;298(5):101893. doi

Insert

mCherry-CRY2–mPKCε-CAT-HA (Prkce Mouse, Synthetic)

Use

Tags

Fused to CRY2, HA, and mCherry

Expression

Mammalian

Mutation

Promoter

CMV

Available since

Jan. 26, 2023

Availability

Academic Institutions and Nonprofits only

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mCherry-MyosinIIC-N-18

Plasmid

#55108

Purpose

Localization: Cytoskeleton, Excitation: 587, Emission: 610

Depositor

Michael Davidson

Article

Ebrahim et al Curr Biol. 2013 Apr 22;23(8):731-6. doi:

Insert

MyosinIIC

Use

Tags

mCherry

Expression

Mammalian

Mutation

Promoter

CMV

Available since

Sept. 17, 2014

Availability

Academic Institutions and Nonprofits only
pYNL-N1_TUBB5

Plasmid

#65678

Purpose

Expresses beta-tubulin-YNL in mammalian cells

Depositor

Yasushi Okada

Article

Takai et al Proc Natl Acad Sci U S A. 2015 Apr 7;112

Insert

beta-tubulin class V

Use

Luciferase

Tags

YNL

Expression

Mammalian

Mutation

Promoter

CMV

Available since

June 18, 2015

Availability

Academic Institutions and Nonprofits only
pTubulin-BC2T

Plasmid

#108970

Purpose

expresses tubulin-BC2-tag in mammalian cells

Depositor

Ulrich Rothbauer

Article

Virant et al Nat Commun. 2018 Mar 2;9(1):930. doi: 10

Insert

a-tubulin

Use

Tags

BC2-tag

Expression

Mammalian

Mutation

Promoter

Available since

June 22, 2018

Availability

Academic Institutions and Nonprofits only

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pEF1α-mouse-retroCHMP3-HA

Plasmid

#154178

Purpose

expresses mouse retroCHMP3 in mammalian cells

Depositor

Wesley Sundquist

Article

Rheinemann et al Cell. 2021 Sep 27. pii: S0092-8674(21)01

Insert

retroCHMP3

Use

Tags

HA

Expression

Mammalian

Mutation

Promoter

EF1alpha

Available since

Oct. 13, 2021

Availability

Academic Institutions and Nonprofits only

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pISH-En2

Plasmid

#84310

Purpose

Use to synthesize riboprobes for in situ hybridization (ISH) probe for En2. Not for gene expression.

Depositor

Peter Mombaerts

Article

Parrilla et al J Comp Neurol. 2016 Oct 1;524(14):2713-3

Insert

En2 (En2 Mouse)

Use

Tags

Expression

Mutation

Promoter

Available since

Nov. 15, 2016

Availability

Academic Institutions and Nonprofits only

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pGEM mouse dbx-1 [TJ#11]

Plasmid

#16269

Depositor

Thomas Jessell

Article

Pierani et al Cell. 1999 Jun 25. 97(7):903-15.

Insert

dbx1 3'UTR (Dbx1 Mouse)

Use

Tags

Expression

Bacterial

Mutation

Promoter

Available since

Feb. 4, 2009

Availability

Academic Institutions and Nonprofits only
Sema4a-Fc-His

Plasmid

#72154

Purpose

Expresses the extracellular region of the Sema4A protein, C-terminally fused to the Fc region of human IgG1 + 6X histidine tag.

Depositor

Woj Wojtowicz

Article

Visser et al Elife. 2015 Dec 3;4. pii: e08149. doi: 1

Insert

Sema4a (Sema4a Mouse)

Use

Tags

Fc-His

Expression

Mammalian

Mutation

Promoter

CMV

Available since

Feb. 26, 2016

Availability

Academic Institutions and Nonprofits only

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pENTR-Casp2

Plasmid

#50023

Purpose

pENTR vector for mouse Caspase-2

Depositor

Jamie Davies

Article

Cachat et al J Biol Eng. 2014 Nov 19;8(1):26. eCollec

Insert

Casp2 (Casp2 Mouse)

Use

Entry vector

Tags

Expression

Mutation

Promoter

Available since

Dec. 10, 2014

Availability

Academic Institutions and Nonprofits only
pAd-Track SIRT1 H355A

Plasmid

#8441

Depositor

Pere Puigserver

Article

Rodgers et al Nature 2005 Mar 3;434(7029):113-8.

Insert

SIRT1 (Sirt1 Mouse)

Use

Adenoviral

Tags

Expression

Mammalian

Mutation

Histidine 355 mutated to Alanine

Promoter

Available since

Dec. 4, 2007

Availability

Academic Institutions and Nonprofits only
pCI-MOR33-1

Plasmid

#22338

Depositor

Hiroaki Matsunami

Article

Saito et al Sci Signal. 2009 . 2(60):ra9.

Insert

MOR33-1 (Olfr653 Mouse)

Use

Tags

Rho

Expression

Mammalian

Mutation

Promoter

Available since

Jan. 6, 2010

Availability

Academic Institutions and Nonprofits only

Enlarge
mEmerald-Cortactin-C-12

Plasmid

#54051

Purpose

Localization: Actin Binding , Excitation: 487, Emission: 509

Depositor

Michael Davidson

Insert

Cortactin

Use

Tags

mEmerald

Expression

Mammalian

Mutation

Promoter

CMV

Available since

July 2, 2014

Availability

Industry, Academic Institutions, and Nonprofits
pBS mEya1 (CT#671)

Plasmid

#13971

Depositor

Cliff Tabin

Article

Heanue et al Mech Dev. 2002 Feb . 111(1-2):75-87.

Insert

Eya1 (Eya1 Mouse)

Use

Tags

Expression

Bacterial

Mutation

Promoter

Available since

Feb. 15, 2007

Availability

Academic Institutions and Nonprofits only
mEgr2/LZRS

Plasmid

#27784

Depositor

David Wiest

Insert

Egr2 (Egr2 Mouse)

Use

Retroviral

Tags

EGFP

Expression

Mammalian

Mutation

Promoter

Available since

March 18, 2011

Availability

Academic Institutions and Nonprofits only
pCI-MOR205-1

Plasmid

#22361

Depositor

Hiroaki Matsunami

Article

Saito et al Sci Signal. 2009 . 2(60):ra9.

Insert

MOR205-1 (Olfr221 Mouse)

Use

Tags

Rho

Expression

Mammalian

Mutation

Promoter

Available since

Jan. 6, 2010

Availability

Academic Institutions and Nonprofits only

Enlarge