We narrowed to 23,570 results for: CHI

1. NLS pEGFP-N3 (1299)

   Plasmid

   #62043

   Purpose

   mammalian expression of NLS-EGFP

   Depositor

   Eric Schirmer

   Article

   Zuleger et al Genome Biol. 2013 Feb 15;14(2):R14. doi:

   Insert

   SV40 NLS
   

   Use

   Tags

   EGFP

   Expression

   Mammalian

   Mutation

   Promoter

   CMV

   Available since

   Jan. 22, 2015

   Availability

   Academic Institutions and Nonprofits only

2. pT2ADW-lox-tdKeima-NES-lox-6011nls(ERK)-P2Av3-RGECO1

   Plasmid

   #162486

   Purpose

   For generating transgenic mice expressing ERK biosensor and RGECO1

   Depositor

   Michiyuki Matsuda

   Article

   Imanishi et al Am J Pathol. 2020 Oct 15. pii: S0002-944

   Insert

   ERK biosensor based on FRET, and RGECO1
   

   Use

   Tags

   Expression

   Mammalian

   Mutation

   Promoter

   CAG

   Available since

   Dec. 2, 2020

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

3. pcDNA3.2/V5 mmu-mir-133a-1

   Plasmid

   #26326

   Depositor

   David Bartel

   Article

   Chiang et al Genes Dev. 2010 May 15. 24(10):992-1009.

   Insert

   mmu-mir-133a-1 (Mir133a-1 Mouse)
   

   Use

   Tags

   Expression

   Mammalian

   Mutation

   Promoter

   Available since

   Sept. 29, 2010

   Availability

   Academic Institutions and Nonprofits only

4. myc-BioID-Rab7

   Plasmid

   #121049

   Purpose

   Expresses myc tagged BioID and human Rab7a in mammalian cells

   Depositor

   Gia Voeltz

   Article

   Hoyer et al Cell. 2018 Sep 20;175(1):254-265.e14. do

   Inserts

   BioID
   Rab7A
   

   Use

   Tags

   myc

   Expression

   Mammalian

   Mutation

   R118G (highly promiscuous form)

   Promoter

   CMV

   Available since

   Feb. 12, 2019

   Availability

   Academic Institutions and Nonprofits only

   

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5. pT2Aneo-ERT2CreERT2

   Plasmid

   #173850

   Purpose

   Encoding Cre recombinase (activated in response to tamoxifen)

   Depositor

   Michiyuki Matsuda

   Article

   Lin et al Life Sci Alliance. 2021 Oct 19;5(1). pii

   Insert

   ERT2CreERT2
   

   Use

   Tags

   Expression

   Mammalian

   Mutation

   Promoter

   Available since

   Oct. 21, 2021

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

6. pCMV Myc rat SAPAP2

   Plasmid

   #40216

   Depositor

   Yoshimi Takai, Yutaka Hata

   Article

   Takeuchi et al J Biol Chem. 1997 May 2;272(18):11943-51

   Insert

   SAPAP2 (Dlgap2 Rat)
   

   Use

   Tags

   myc

   Expression

   Mammalian

   Mutation

   H137Q and K806R

   Promoter

   CMV

   Available since

   Nov. 28, 2012

   Availability

   Academic Institutions and Nonprofits only

7. LentiU6-hMYOD1 gRNA_1-MS2-Puro

   Plasmid

   #192673

   Purpose

   Lentiviral expression of sgRNA targeting hMYOD1 promoter to activate human MYOD1 transcription

   Depositor

   Jeffrey Miner

   Article

   Omachi et al PLoS One. 2022 Jun 28;17(6):e0270008. do

   Insert

   Human MYOD1 activating gRNA #1 (MYOD1 Human)
   

   Use

   CRISPR and Lentiviral

   Tags

   Expression

   Mammalian

   Mutation

   Promoter

   U6

   Available since

   Dec. 15, 2022

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

8. pCMV_nLightR

   Plasmid

   #217657

   Purpose

   Expresses norepinephrine indicator nLightR under mammalian promoter

   Depositor

   Tommaso Patriarchi

   Article

   Kagiampaki et al Nat Methods. 2023 Sep;20(9):1426-1436. d

   Insert

   nLightR
   

   Use

   Tags

   Expression

   Mammalian

   Mutation

   Promoter

   Available since

   March 28, 2024

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

9. pGBW-m4046757

   Plasmid

   #145657

   Purpose

   Bacterial Expression plasmid for SARS-CoV-2 RNA-dependent RNA polymerase

   Depositor

   Benjie Chen, Ginkgo Bioworks

   Insert

   SARS-CoV-2 RNA-dependent RNA polymerase (ORF1ab Escherichia coli str. K-12 substr. MG1655; Severe acute respiratory syndrome coronavirus 2)
   

   Use

   Tags

   Cleavable TEV;6xHIS

   Expression

   Bacterial

   Mutation

   Escherichia coli recode 1

   Promoter

   Promoter | pT7 ; Operator | lacO ; RBS | T7_rbs ;…

   Available since

   April 29, 2020

   Availability

   Industry, Academic Institutions, and Nonprofits

   

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10. pLV-SI-121

    Plasmid

    #131126

    Purpose

    A-to-G base editing reporter

    Depositor

    Nozomu Yachie

    Article

    Sakata et al Nat Biotechnol. 2020 Jun 1. pii: 10.1038

    Insert

    mutant EGFP
    

    Use

    Lentiviral and Synthetic Biology

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    CMV promoter

    Available since

    Sept. 23, 2019

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

11. pBS-KLF7-200-GFPfr1

    Plasmid

    #169799

    Purpose

    THe donor vector for the KLF7 gene locus.

    Depositor

    Natsuko Chiba

    Article

    Yoshino et al Sci Rep. 2019 Feb 7;9(1):1644. doi: 10.1

    Insert

    Donor sequence to KLF7 neighbouring region for HR
    

    Use

    Hr donor vector for human actb gene.

    Tags

    Expression

    Mutation

    Partial sequence of GFP is inserted the sequence …

    Promoter

    Available since

    Aug. 4, 2021

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

12. LentiCRISPRv2-KLF7-1

    Plasmid

    #169797

    Purpose

    Expresses Cas9 and guide RNA for the site neighbouring KLF7 gene.

    Depositor

    Natsuko Chiba

    Article

    Yoshino et al Sci Rep. 2019 Feb 7;9(1):1644. doi: 10.1

    Insert

    guide RNA for KLF7 neighbouring region
    

    Use

    CRISPR and Lentiviral

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    U6

    Available since

    June 11, 2021

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

13. 3xFLAG-dCas9/pMSCVpuro

    Plasmid

    #134983

    Purpose

    Expresses 3xFLAG-dCas9 in mammalian cells for enChIP analysis to purify specific genomic regions of interest.

    Depositor

    Hodaka Fujii

    Article

    Yuno et al Biol Methods Protoc. 2021 Jul 9;6(1):bpa

    Insert

    3xFLAG-dCas9
    

    Use

    CRISPR and Retroviral

    Tags

    3xFLAG tag and NLS

    Expression

    Mammalian

    Mutation

    human codon-optimized, D10A + H840A

    Promoter

    LTR

    Available since

    April 22, 2024

    Availability

    Academic Institutions and Nonprofits only

    

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14. pMRX-INU-TEX264 LIR4A-FLAG

    Plasmid

    #128259

    Purpose

    Expresses TEX264 LIR4A in mammalian cells

    Depositor

    Noboru Mizushima

    Article

    Chino et al Mol Cell. 2019 Apr 11. pii: S1097-2765(1

    Insert

    TEX264 LIR4A (TEX264 Human)
    

    Use

    Retroviral

    Tags

    FLAG

    Expression

    Mammalian

    Mutation

    Changed FEEL (aa 273-276) to alanines

    Promoter

    CMV

    Available since

    July 26, 2019

    Availability

    Academic Institutions and Nonprofits only

    

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15. THI VP64 pALD4 T-

    Plasmid

    #161481

    Purpose

    Non targeting control for CRISPR/RNA scaffold based transcription regulation in Pichia pastoris BB3rN_pTEF2_dCAS9(3xFLAG_SV40NLS)_pPOR1_MS2bind_only_SV40NLS_pALD4_THi11_T-_gRNA_MS2

    Depositor

    Brigitte Gasser, Diethard Mattanovich

    Article

    Baumschabl et al ACS Synth Biol. 2020 Dec 18;9(12):3202-3

    Inserts

    dCAS9
    MS2-VP64
    gRNA (Non targeting control NTC)
    

    Use

    Tags

    Expression

    Yeast

    Mutation

    Promoter

    pALD4, pPOR1, and pTEF2

    Available since

    March 15, 2021

    Availability

    Academic Institutions and Nonprofits only

    

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16. pMpGE_En01

    Plasmid

    #71534

    Purpose

    Used to construct a gRNA expression cassette for CRISPR/Cas9 genome editing in Marchantia polymorpha

    Depositor

    Ikuko Hara-Nishimura, Takayuki Kohchi

    Article

    Sugano et al PLoS One. 2018 Oct 31;13(10):e0205117. d

    Type

    Empty backbone

    Use

    CRISPR

    Tags

    Expression

    Mutation

    Promoter

    Available since

    Dec. 22, 2015

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

17. Tmem214 pmRFP-N2 (1348)

    Plasmid

    #62047

    Purpose

    mammalian expression of nuclear envelope transmembrane protein

    Depositor

    Eric Schirmer

    Article

    Wilkie et al Mol Cell Proteomics. 2011 Jan;10(1):M110

    Insert

    Tmem214 (TMEM214 Human)
    

    Use

    Tags

    mRFP

    Expression

    Mammalian

    Mutation

    Promoter

    CMV

    Available since

    Jan. 23, 2015

    Availability

    Academic Institutions and Nonprofits only

18. pMM2-hPMCA2w/b

    Plasmid

    #47583

    Depositor

    Emanuel Strehler

    Article

    Chicka et al J Biol Chem. 2003 May 16;278(20):18464-7

    Insert

    PMCA2w/b (ATP2B2 Human)
    

    Use

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    SV40

    Available since

    Oct. 4, 2013

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

19. pMM2-hPMCA2z/b

    Plasmid

    #47581

    Depositor

    Emanuel Strehler

    Article

    Chicka et al J Biol Chem. 2003 May 16;278(20):18464-7

    Insert

    PMCA2z/b (ATP2B2 Human)
    

    Use

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    SV40

    Available since

    Oct. 4, 2013

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

20. pPB-CAG-Tfe3::ERT2-pgk-hph

    Plasmid

    #48756

    Purpose

    PiggyBac backbone, CAG promoter driving murine Tfe3-ERT2 fusion, Hygromycin selection

    Depositor

    Austin Smith

    Article

    Betschinger et al Cell. 2013 Apr 11;153(2):335-47. doi: 10

    Insert

    Tfe3::ERT2 (Tcfe3 Mouse)
    

    Use

    Piggybac

    Tags

    ERT2

    Expression

    Mammalian

    Mutation

    Promoter

    CAG

    Available since

    July 27, 2016

    Availability

    Academic Institutions and Nonprofits only

21. Luc-SIRT1 mutant 3'UTR

    Plasmid

    #20380

    Depositor

    Charles Lowenstein

    Article

    Yamakuchi et al Proc Natl Acad Sci U S A. 2008 Sep 9. 10

    Insert

    SIRT1 (SIRT1 Human)
    

    Use

    Luciferase

    Tags

    Expression

    Mutation

    mutant 3'UTR of SIRT1 (ACACCCACCAAGGACCATTAG…

    Promoter

    Available since

    May 1, 2009

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

22. pcDNA3.2/V5 hsa-mir-192

    Plasmid

    #26311

    Depositor

    David Bartel

    Article

    Chiang et al Genes Dev. 2010 May 15. 24(10):992-1009.

    Insert

    hsa-mir-192 (MIR192 Human)
    

    Use

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    Available since

    Sept. 29, 2010

    Availability

    Academic Institutions and Nonprofits only

23. pcDNA3.2/V5 hsa-mir-124

    Plasmid

    #26306

    Depositor

    David Bartel

    Article

    Chiang et al Genes Dev. 2010 May 15. 24(10):992-1009.

    Insert

    hsa-mir-124 (MIR124-1 Human)
    

    Use

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    Available since

    Sept. 29, 2010

    Availability

    Academic Institutions and Nonprofits only

24. pDONR221 CARD8

    Plasmid

    #169962

    Purpose

    pDONR221 for Gateway shuttling. Contains the CARD8 T60 isoform (no stop codon)

    Depositor

    Daniel Bachovchin

    Article

    Sharif et al Immunity. 2021 Jul 13;54(7):1392-1404.e1

    Insert

    CARD8 (CARD8 Human)
    

    Use

    Gateway cloning vector

    Tags

    Expression

    Mutation

    Promoter

    Available since

    July 28, 2021

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

25. PKG I alpha dNPS

    Plasmid

    #16391

    Depositor

    Bernard Weinstein

    Article

    Deguchi et al Mol Cancer Ther. 2002 Aug . 1(10):803-9.

    Insert

    PKG1 alpha (PRKG1 Human)
    

    Use

    Tags

    HA

    Expression

    Mammalian

    Mutation

    N-terminal pseudosubstrate domain deletion: delet…

    Promoter

    Available since

    April 28, 2009

    Availability

    Academic Institutions and Nonprofits only

26. Tmem214 pEGFP-N2 (1072)

    Plasmid

    #62026

    Purpose

    mammalian expression of nuclear envelope transmembrane protein

    Depositor

    Eric Schirmer

    Article

    Wilkie et al Mol Cell Proteomics. 2011 Jan;10(1):M110

    Insert

    Tmem214 (TMEM214 Human)
    

    Use

    Tags

    EGFP

    Expression

    Mammalian

    Mutation

    Promoter

    CMV

    Available since

    Jan. 28, 2015

    Availability

    Academic Institutions and Nonprofits only

27. pCMV-YdLight1

    Plasmid

    #125705

    Purpose

    Expresses the yellow genetically encoded dopamine sensor YdLight1 in mammalian cells.

    Depositor

    Lin Tian

    Article

    Patriarchi et al Nat Methods. 2020 Nov;17(11):1147-1155.

    Insert

    YdLight1
    

    Use

    Tags

    Flag tag

    Expression

    Mammalian

    Mutation

    Promoter

    CMV

    Available since

    July 1, 2021

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

28. px330-UFM1 sgRNA2

    Plasmid

    #134634

    Purpose

    contains sgRNA targeting human UFM1 for gene knockout

    Depositor

    Yihong Ye

    Article

    Wang et al Cell Res. 2020 Jan;30(1):5-20. doi: 10.1

    Insert

    UFM1 sgRNA2 (UFM1 Human)
    

    Use

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    Available since

    Nov. 19, 2019

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

29. px330-UFM1 sgRNA1

    Plasmid

    #134633

    Purpose

    contains sgRNA targeting human UFM1 for gene knockout

    Depositor

    Yihong Ye

    Article

    Wang et al Cell Res. 2020 Jan;30(1):5-20. doi: 10.1

    Insert

    UFM1 sgRNA1 (UFM1 Human)
    

    Use

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    Available since

    Nov. 19, 2019

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

30. pcDNA3.2/V5 hsa-mir-205

    Plasmid

    #26312

    Depositor

    David Bartel

    Article

    Chiang et al Genes Dev. 2010 May 15. 24(10):992-1009.

    Insert

    hsa-mir-205 (MIR205 Human)
    

    Use

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    Available since

    Sept. 29, 2010

    Availability

    Academic Institutions and Nonprofits only