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pAWP78-PVCpnf_pvc13-Ad5RGDPK7_Δpvc10

Plasmid

#198449

Purpose

Produces PVCs retargeted with expanded tropism hAd5 knob (RGD and PK7 insertions); also contains truncated spike tip protein (Pvc10).

Depositor

Feng Zhang

Article

Kreitz et al Nature. 2023 Apr;616(7956):357-364. doi:

Insert

PVCpnf_pvc13-Ad5RGDPK7_Δpvc10

Expression

Bacterial

Available Since

March 29, 2023

Availability

Academic Institutions and Nonprofits only

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RFP-KIF3A-HL

Plasmid

#51397

Purpose

expression of RFP-tagged amino acid 369-701 (headless) of KIF3A in mammalian cells

Depositor

Trina Schroer

Article

Brown et al Traffic. 2005 Dec;6(12):1114-24.

Insert

KIF3A (Kif3a Mouse)

Tags

RFP

Expression

Mammalian

Promoter

CMV

Available Since

Feb. 20, 2014

Availability

Academic Institutions and Nonprofits only
pRRL-GFPGO2(NGG)-PGK-Neo

Plasmid

#136891

Purpose

Lentivirus for base editing activatable GFP expression in mammalian cells

Depositor

Lukas Dow

Article

Katti et al Nucleic Acids Res. 2020 Feb 29. pii: 576

Insert

GFPGO2(NGG)

Use

Lentiviral

Tags

2xNLS

Mutation

ATG>ACG

Promoter

SFFV

Available Since

Feb. 10, 2020

Availability

Academic Institutions and Nonprofits only

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pCS-H2B-citrine

Plasmid

#53752

Purpose

Contains fusion of human Histone H2B to citrine.

Depositor

Sean Megason

Article

Megason et al Methods Mol Biol. 2009;546:317-32. doi:

Insert

histone H2B (H2BC21 Human)

Tags

citrine

Expression

Mammalian

Promoter

simian CMV IE94

Available Since

July 8, 2014

Availability

Academic Institutions and Nonprofits only

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pFH6_new

Plasmid

#105866

Purpose

more efficient version of pFH6; Subcloning of any sgRNA via BbsI site

Depositor

Andreas Weber

Article

Hahn et al Bio Protoc. 2017 Jul 5;7(13):e2384. doi:

Insert

U6-26p::sgRNA scaffold

Use

CRISPR and Synthetic Biology; Subcloning

Expression

Plant

Available Since

April 27, 2018

Availability

Academic Institutions and Nonprofits only

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pWJB30

Plasmid

#126505

Purpose

Salmonella typhimurium diguanylate cyclase AdrA cloned as AdrA-8xHis in pBAD30

Depositor

Ute Romling

Article

Simm et al Mol Microbiol. 2004 Aug;53(4):1123-34. d

Insert

adrA-8xHis

Tags

8xHis

Expression

Bacterial

Promoter

PBAD30

Available Since

Aug. 6, 2019

Availability

Academic Institutions and Nonprofits only

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pEF-pIgH-ESCargo(FTV,Orange)

Plasmid

#140161

Purpose

Expresses a regulatable secretory cargo for mammalian cells

Depositor

Benjamin Glick

Article

Casler et al Mol Biol Cell. 2020 Oct 28:mbcE20090591.

Insert

piGH-FTVNTT-E2-Orange-FKBP(LV)(C22V)

Tags

ER signal sequence (MGWSCIILFLVATATGAHS) (N termi…

Expression

Mammalian

Promoter

EF-1a

Available Since

March 27, 2020

Availability

Academic Institutions and Nonprofits only

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pLenti-hU6-mU6-BlasGO

Plasmid

#136906

Purpose

Lentivirus for base editing activatable Blasticidin resistance gene expression in mammalian cells. All in one vector with sgBlasGO.

Depositor

Lukas Dow

Article

Katti et al Nucleic Acids Res. 2020 Feb 29. pii: 576

Insert

BlasCyGO

Use

Lentiviral

Mutation

ATG>ACG

Promoter

SFFV

Available Since

Feb. 10, 2020

Availability

Academic Institutions and Nonprofits only

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pCIneo-lambdaN-HA-HsTNRC6Biso3_C

Plasmid

#146088

Purpose

Mammalian Expression of HsTNRC6Biso3

Depositor

Elisa Izaurralde

Article

Lazzaretti et al RNA. 2009 Jun . 15(6):1059-66.

Insert

HsTNRC6Biso3 (TNRC6B Human)

Expression

Mammalian

Mutation

AA 3-188 and AA residues 242-1029 (with N242D) co…

Available Since

Feb. 25, 2022

Availability

Academic Institutions and Nonprofits only

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pLVX-3XMyc-mTriml1

Plasmid

#51395

Depositor

Narry Kim

Article

Kwon et al Nat Struct Mol Biol. 2013 Sep;20(9):1122

Insert

Triml1 (Triml1 Mouse)

Use

Lentiviral

Tags

3XMyc

Expression

Mammalian

Promoter

EF1alpha

Available Since

March 31, 2014

Availability

Academic Institutions and Nonprofits only

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pT7-EGFP-C1-HsTNRC6Biso1

Plasmid

#25034

Depositor

Elisa Izaurralde

Article

Lazzaretti et al RNA. 2009 Jun . 15(6):1059-66.

Insert

TNRC6B (TNRC6B Human)

Tags

EGFP

Expression

Mammalian

Available Since

June 22, 2010

Availability

Academic Institutions and Nonprofits only

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Mouse NCKX3 (pcDNA3.1+)

Plasmid

#75204

Purpose

Mammalian expression of SLC24A3

Depositor

Jonathan Lytton

Article

Kraev et al J Biol Chem. 2001 Jun 22;276(25):23161-7

Insert

SLC24A3 (Slc24a3 Mouse)

Expression

Mammalian

Promoter

CMV

Available Since

June 21, 2016

Availability

Academic Institutions and Nonprofits only

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pCB6HindIII-rat FcRn

Plasmid

#121671

Purpose

Expresses rat neonatal Fc receptor in mammalian cells

Depositor

Pamela Bjorkman

Article

Tesar et al Traffic. 2006 Sep;7(9):1127-42.

Insert

rat neonatal Fc receptor (Fcgrt Rat)

Expression

Mammalian

Available Since

Feb. 20, 2019

Availability

Academic Institutions and Nonprofits only

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pBigTB-FlpERT2

Plasmid

#149435

Purpose

tamoxifen-inducible Flp recombinase

Depositor

Primo Schaer

Article

Schwarz et al F1000Res. 2020 Sep 9;9:1112. doi: 10.126

Insert

Flp-ERT2 fusion protein

Use

Cre/Lox and Mouse Targeting

Tags

ERT2

Expression

Mammalian

Promoter

CAG promoter

Available Since

Sept. 10, 2020

Availability

Academic Institutions and Nonprofits only

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pRL_yEGFP_hc

Plasmid

#98746

Purpose

Reporter plasmid for the PhiReX system. Encodes the reporter gene yEGFP under the control of the CYC1 minimal promoter with upstream synTALE-DBS.

Depositor

Bernd Müller-Röber

Article

Hochrein et al Nucleic Acids Res. 2017 Sep 6;45(15):919

Insert

Yeast-enhanced green fluorescent protein

Use

Synthetic Biology

Expression

Yeast

Promoter

synthetic

Available Since

Aug. 14, 2017

Availability

Academic Institutions and Nonprofits only

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Phat2-hs-cPla2-C2-EGFP-GH

Plasmid

#187088

Purpose

Protein Expression

Depositor

Philipp Niethammer

Article

Shen et al Proc Natl Acad Sci U S A. 2022 Jan 4;119

Insert

ENSG00000116711 (PLA2G4A Human)

Tags

EGFP

Expression

Bacterial

Available Since

Aug. 3, 2022

Availability

Academic Institutions and Nonprofits only

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pRK5-mFzd10-1D4

Plasmid

#42272

Depositor

Chris Garcia, Jeremy Nathans

Article

Yu et al Development. 2012 Dec 1;139(23):4383-94.

Insert

Frizzled 10 (Fzd10 Mouse)

Tags

1D4

Expression

Mammalian

Available Since

March 28, 2013

Availability

Academic Institutions and Nonprofits only
BC-A1-002

Plasmid

#78689

Purpose

A strong promoter drives the expression of wild type luxR, which regulates expression of GFP under the control of a strong RBS.

Depositor

Brian Chow

Article

Magaraci et al ACS Synth Biol. 2016 Apr 25.

Insert

BBa_J23100-BBa_B0034-luxR-Terminator-pLux-BBa_B0034-GFP-Terminator

Use

Synthetic Biology

Available Since

Jan. 31, 2017

Availability

Academic Institutions and Nonprofits only

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LR (bact: HyPer)

Plasmid

#162069

Purpose

Used for the diffuse visualization of H2O2 production in cells.

Depositor

Philipp Niethammer

Article

Enyedi et al Nat Cell Biol. 2013 Sep;15(9):1123-30. d

Insert

beta actin 2 (actb2 Zebrafish)

Use

Gateway donor vector

Tags

HyPer

Promoter

T3

Available Since

March 2, 2021

Availability

Academic Institutions and Nonprofits only

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BC-A1-006

Plasmid

#78693

Purpose

A weak promoter drives the expression of wild type luxR, which regulates expression of GFP under the control of a strong RBS.

Depositor

Brian Chow

Article

Magaraci et al ACS Synth Biol. 2016 Apr 25.

Insert

BBa_J23109-BBa_B0034-luxR-Terminator-pLux-BBa_B0034-GFP-Terminator

Use

Synthetic Biology

Available Since

Feb. 2, 2017

Availability

Academic Institutions and Nonprofits only

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