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PRKDC gRNA (BRDN0001149438)

Plasmid

#77863

Purpose

3rd generation lentiviral gRNA plasmid targeting human PRKDC

Depositor

John Doench, David Root

Publication

Doench et al Nat Biotechnol. 2016 Jan 18. doi: 10.103

Insert

PRKDC (PRKDC Human)

Use

CRISPR and Lentiviral

Expression

Mammalian

Promoter

hU6

Available since

July 6, 2016

Availability

Academic Institutions and Nonprofits only

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pAAV-CAG-iGluSnFR4f-NGR-WPRE

Plasmid

#234452

Purpose

AAV-mediated expression of glutamate sensor with improved sensitivity and fast deactivation kinetics (4f = fast); NGR vector matches or outperforms PDGFR in all conditions tested.

Depositor

GENIE Project

Publication

Aggarwal et al Nat Methods. 2026 Feb;23(2):417-425. doi

Insert

iGluSnFR4f-NGR

Use

AAV

Tags

IgK chain and Myc epi tag-NGR GPI anchor

Expression

Mammalian

Promoter

CAG

Available since

March 20, 2025

Availability

Academic Institutions and Nonprofits only

Cited by

1 Article

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pGEX6P1-mClover3-anti-mouse-IgG(kappa)-nanobody

Plasmid

#138128

Purpose

Expresses GST-tagged mClover3-anti-mouse-IgG(kappa)-nanobody in E. coli cells.

Depositor

Kazuhisa Nakayama

Publication

Katoh et al Mol Biol Cell. 2020 Jul 29:mbcE20040250.

Insert

mClover3-anti-mouse-IgG(kappa)-nanobody

Tags

GST

Expression

Bacterial

Available since

July 30, 2020

Availability

Academic Institutions and Nonprofits only

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p6608 MSCV-IP N-HAonly CHAF1B

Plasmid

#34901

Depositor

Peter Howley

Publication

White et al Proc Natl Acad Sci U S A. 2012 Jan 9.

Insert

CHAF1B (CHAF1B Human)

Use

Retroviral

Tags

HA

Expression

Mammalian

Promoter

MSCV LTR

Available since

Feb. 8, 2012

Availability

Academic Institutions and Nonprofits only

Cited by

1 Article

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BFP

Plasmid

#201383

Purpose

Control nucleocytoplasmic transport probe

Depositor

Pere Roca-Cusachs

Publication

Andreu et al Nat Cell Biol. 2022 Jun;24(6):896-905. d

Has service

50 µg Transfection-Ready DNA and 100 µg Transfection-Ready DNA

Insert

BFP

Expression

Mammalian

Available since

July 10, 2023

Availability

Academic Institutions and Nonprofits only

Cited by

1 Article

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pTol2-10xUAS-Voltron-ST

Plasmid

#119040

Purpose

UAS-driven expresion of soma-localized Voltron in zebrafish

Depositor

Eric Schreiter

Publication

Abdelfattah et al Science. 2019 Aug 1. pii: science.aav641

Insert

Voltron-ST

Use

Zebrafish expression

Promoter

E1b

Available since

Dec. 7, 2018

Availability

Academic Institutions and Nonprofits only

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pXFokI-dCas9

Plasmid

#60901

Purpose

Dual Expression Vector for FokI-dCas9 and gRNA

Depositor

Bruce Conklin

Publication

Miyaoka et al Sci Rep. 2016 Mar 31;6:23549. doi: 10.10

Type

Empty backbone

Use

CRISPR

Tags

3XFLAG

Expression

Mammalian

Promoter

CBh and U6

Available since

March 31, 2016

Availability

Academic Institutions and Nonprofits only

Cited by

1 Article

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FLAG-BCL9

Plasmid

#113759

Purpose

For the expression of FLAG-BCL9 in mammalian cells

Depositor

Mariann Bienz

Publication

de la Roche et al BMC Cancer. 2008 Jul 15;8:199. doi: 10.1

Insert

BCL9 (BCL9 Human)

Tags

Flag

Expression

Mammalian

Available since

Aug. 8, 2018

Availability

Academic Institutions and Nonprofits only

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pBSK

Plasmid

#67504

Purpose

Express the tRNALys scaffold

Depositor

Luc Ponchon

Publication

Ponchon et al Nat Protoc. 2009;4(6):947-59. doi: 10.10

Insert

modified tRNALys human

Expression

Bacterial

Promoter

lpp

Available since

Sept. 15, 2015

Availability

Academic Institutions and Nonprofits only

Cited by

1 Article
pBAD-D4

Plasmid

#37473

Depositor

Amy Palmer, Roger Tsien

Publication

Palmer et al Chem Biol. 2006 May;13(5):521-30.

Insert

D4 second generation cameleon (calcium sensor)

Expression

Bacterial

Promoter

Ara

Available since

Oct. 11, 2012

Availability

Academic Institutions and Nonprofits only

Cited by

3 Articles
pSM1-1-1

Plasmid

#15995

Depositor

Morris Birnbaum

Publication

Birnbaum et al Cell. 1989 Apr 21. 57(2):305-15.

Insert

GLUT4 (Slc2a4 Rat)

Expression

Bacterial

Available since

Oct. 26, 2007

Availability

Academic Institutions and Nonprofits only
HSPA8_pLX307

Plasmid

#98343

Purpose

Lentiviral expression of HSPA8

Depositor

William Hahn, Sefi Rosenbluh

Publication

Rosenbluh et al Cell Syst. 2016 Sep 28;3(3):302-316.e4.

Insert

HSPA8 (HSPA8 Human)

Use

Lentiviral

Tags

V5

Expression

Mammalian

Promoter

E1Fa

Available since

Aug. 23, 2017

Availability

Academic Institutions and Nonprofits only

Cited by

2 Articles

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pcDNA3.1-Flag HBx (G124L, I127A)

Plasmid

#42598

Depositor

Ding Xue

Publication

Geng et al Proc Natl Acad Sci U S A. 2012 Nov 6;109

Has service

50 µg Transfection-Ready DNA and 100 µg Transfection-Ready DNA

Insert

Hepatitis B virus protein X (G124L, I127A)

Tags

Flag

Expression

Mammalian

Mutation

G124L I127A

Available since

March 22, 2013

Availability

Academic Institutions and Nonprofits only
YF-Rac1(DN)

Plasmid

#20151

Depositor

Tobias Meyer

Publication

Inoue et al Nat Methods. 2005 Jun . 2(6):415-8.

Has service

50 µg Transfection-Ready DNA and 100 µg Transfection-Ready DNA

Insert

YFP-FKBP-Rac1(DN) (RAC1 Human)

Expression

Mammalian

Mutation

dominant negative mutation

Available since

March 23, 2009

Availability

Academic Institutions and Nonprofits only

Cited by

2 Articles

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pZmUBQ:GW

Plasmid

#132358

Purpose

Expression of CDS from the Zea mays ubiquitin promotor

Depositor

Paul Schulze-Lefert

Publication

Saur et al Plant Methods. 2019 Oct 24;15:118. doi:

Type

Empty backbone

Tags

YFP

Expression

Plant

Promoter

pZmUBQ

Available since

Oct. 24, 2019

Availability

Academic Institutions and Nonprofits only

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pCAG-loxSTOPlox-LynEGFP

Plasmid

#133922

Purpose

Reporter expressing membrane targeted EGFP for visualization of neuronal morphology upon Cre-based recombination

Depositor

Frank Bradke, Sebastián Dupraz

Publication

Dupraz et al Curr Biol. 2019 Nov 18;29(22):3874-3886.

Has service

50 µg Transfection-Ready DNA and 100 µg Transfection-Ready DNA

Insert

LynEGFP

Expression

Mammalian

Available since

June 10, 2021

Availability

Academic Institutions and Nonprofits only

Cited by

1 Article

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pAAV-EF1a-iDreV

Plasmid

#140136

Purpose

can be used to generate AAV virus that will express light-inducible site-specific iDreV recombinase

Depositor

Hongkui Zeng

Publication

Yao et al Nat Methods. 2020 Mar 23. pii: 10.1038/s

Has service

AAV1 and AAV PHP.eB

Insert

iDreV

Use

AAV

Promoter

EF1a

Available since

April 23, 2020

Availability

Academic Institutions and Nonprofits only

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Cav2.2e[a10,Δ18a, Δ24a, 31a, 37b, 46]

Plasmid

#26567

Depositor

Diane Lipscombe

Publication

Lin et al Neuron. 1997 Jan . 18(1):153-66.

Insert

calcium channel alpha-1B subunit (Cacna1b Rat)

Tags

6XHis and V5

Expression

Mammalian

Mutation

Contains exons a10, 31a, 37b, and 46. Deletion…

Available since

Nov. 29, 2010

Availability

Academic Institutions and Nonprofits only

Cited by

2 Articles
p255 pPr-CREM/Ins/CMV-STOP-luc

Plasmid

#8408

Purpose

Modified cre expressed from probasin, separated from the Cre-dependent CMV-STOP-luc cassette by insulators.

Depositor

Jeffrey Green

Publication

Kaczmarczyk et al Nucleic Acids Res 2001 Jun 15;29(12):E56

Insert

CREM/insulators/lox/luciferase

Use

Cre/Lox

Expression

Mammalian

Mutation

modified Cre

Promoter

Probasin, CMV

Available since

April 26, 2006

Availability

Academic Institutions and Nonprofits only
LentiCRISPR v2-sgLKB1 clone1

Plasmid

#162125

Purpose

Lentiviral sgRNA plasmid targeting human LKB1

Depositor

Boyi Gan

Publication

Lee et al Nat Cell Biol. 2020 Feb;22(2):225-234. d

Insert

sgLKB1 (STK11 Human)

Use

Lentiviral

Available since

Jan. 26, 2021

Availability

Academic Institutions and Nonprofits only

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