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pICH88103

Plasmid

#50273

Depositor

Sylvestre Marillonnet, Nicola Patron

Article

Engler et al ACS Synth Biol. 2014 Nov 21;3(11):839-43

Insert

Promoter + 5'UTR, ocs, (A. tumefaciens) + 5'UTR, omega (Tobacco Mosaic Virus)

Use

Plant expression

Mutation

BsaI/BbsI sites removed by point-mutation

Available Since

Feb. 18, 2014

Availability

Academic Institutions and Nonprofits only

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pICH87655

Plasmid

#50279

Depositor

Sylvestre Marillonnet, Nicola Patron

Article

Engler et al ACS Synth Biol. 2014 Nov 21;3(11):839-43

Insert

promoter, cab2 (AT1g29930, A. thaliana) and 5'UTR, omega (Tobacco Mosaic Virus)

Use

Plant expression

Mutation

BsaI/BbsI sites removed by point-mutation

Available Since

Feb. 18, 2014

Availability

Academic Institutions and Nonprofits only

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lentiCRISPR v2

Plasmid

#52961

Purpose

Replaces original lentiCRISPRv1 (Addgene Plasmid 49535) and produces ~10-fold higher titer virus. 3rd generation lentiviral backbone.

Depositor

Feng Zhang

Article

Sanjana et al Nat Methods. 2014 Aug;11(8):783-4. doi:

Inserts

Cas9
Puromycin resistance

Use

CRISPR and Lentiviral

Tags

FLAG

Expression

Mammalian

Promoter

EFS-NS

Available Since

May 8, 2014

Availability

Academic Institutions and Nonprofits only

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LRG (Lenti_sgRNA_EFS_GFP)

Plasmid

#65656

Purpose

Lentiviral introduction of sgRNA constitute expression linked with GFP marker into mammalian cell line.

Depositor

Christopher Vakoc

Article

Shi et al Nat Biotechnol. 2015 May 11. doi: 10.103

Type

Empty backbone

Use

CRISPR and Lentiviral

Expression

Mammalian

Promoter

U6 promoter-driven sgRNA expression and EFS promo…

Available Since

June 8, 2015

Availability

Academic Institutions and Nonprofits only

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pEV53D

Plasmid

#44168

Depositor

John Olsen

Article

Patel et al J Gene Med. 2013 Jan;15(1):51-62. doi: 1

Insert

Equine infectious anemia virus (EIAV) gag-pol,rev, and S2

Use

Lentiviral

Expression

Mammalian

Mutation

A131T mutation in gag; Deletion of viral LTR sequ…

Promoter

CMV

Available Since

April 24, 2013

Availability

Academic Institutions and Nonprofits only

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pHAGE-CMV-GFP-W

Plasmid

#196046

Purpose

lentiviral expression of the green fluorescent protein driven by cytomegalovirus virus promoter

Depositor

Darrell Kotton

Article

Wilson et al J Clin Invest. 2010 Jan . 120(1):379-89.

Type

Empty backbone

Use

Lentiviral

Tags

eGFP

Promoter

CMV

Available Since

Feb. 3, 2023

Availability

Academic Institutions and Nonprofits only

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SpyCatcher003-mi3

Plasmid

#159995

Purpose

Expresses SpyCatcher003-mi3 in bacterial cells as virus-like particle platform for plug-and-display vaccination

Depositor

Mark Howarth

Article

Rahikainen et al Angew Chem Int Ed Engl. 2020 Sep 4. doi:

Insert

SpyCatcher003-mi3

Tags

C-Tag

Expression

Bacterial

Promoter

T7

Available Since

Nov. 9, 2020

Availability

Academic Institutions and Nonprofits only

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pCMV-PE2-SpRY

Plasmid

#159979

Purpose

Prime editing in mammalian cells

Depositor

Yongsub Kim

Article

Kweon et al Mol Ther. 2021 Feb 23. pii: S1525-0016(2

Insert

PE2-SpRY

Tags

BpSV40 NLS

Expression

Mammalian

Promoter

CMV

Available Since

Nov. 12, 2020

Availability

Academic Institutions and Nonprofits only

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pCMV-PE2-VQR

Plasmid

#159982

Purpose

Prime editing in mammalian cells

Depositor

Yongsub Kim

Article

Kweon et al Mol Ther. 2021 Feb 23. pii: S1525-0016(2

Insert

PE2-VQR

Tags

BpSV40 NLS

Expression

Mammalian

Promoter

CMV

Available Since

Nov. 12, 2020

Availability

Academic Institutions and Nonprofits only

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pEarleyGate103 mmPing20F

Plasmid

#140007

Purpose

mmPing20F activation tag

Depositor

Charles Nathan Hancock

Article

Johnson et al Plant Direct. 2021 Jan 19;5(1):e00300. d

Insert

mmPing20F

Expression

Plant

Available Since

Jan. 20, 2021

Availability

Academic Institutions and Nonprofits only

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pCMV-PE2-VRQR

Plasmid

#159983

Purpose

Prime editing in mammalian cells

Depositor

Yongsub Kim

Article

Kweon et al Mol Ther. 2021 Feb 23. pii: S1525-0016(2

Insert

PE2-VRQR

Tags

BpSV40 NLS

Expression

Mammalian

Promoter

CMV

Available Since

Nov. 12, 2020

Availability

Academic Institutions and Nonprofits only

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CVS-N2c-nl.EGFP-FlpO

Plasmid

#172379

Purpose

Production of RVdG-CVS-N2c viral vectors for cell-type specific trans-synaptic retrograde labeling.

Depositor

Yoav Ben-Simon, Peter Jonas

Article

Sumser et al Elife. 2022 Aug 30;11:e79848. doi: 10.75

Insert

nuclear-localized EGFP + FlpO

Use

Neurotropic virus

Available Since

Sept. 30, 2021

Availability

Academic Institutions and Nonprofits only

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pJEP221-AAV-RSV-GFP-pA

Plasmid

#62919

Purpose

AAV vector backbone designed to express GFP from a RSV promoter. It also contains a poly-Adenylation Signal (pA)

Depositor

Jonathan Ploski

Article

Holehonnur et al Mol Brain. 2015 Feb 24;8:12. doi: 10.118

Insert

Green Fluorescent protein (GFP)

Use

AAV

Promoter

Rous Sarcoma Virus(RSV)

Available Since

March 15, 2022

Availability

Academic Institutions and Nonprofits only

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pCMV-PE2-SpG

Plasmid

#159978

Purpose

Prime editing in mammalian cells

Depositor

Yongsub Kim

Article

Kweon et al Mol Ther. 2021 Feb 23. pii: S1525-0016(2

Insert

PE2-SpG

Tags

BpSV40 NLS

Expression

Mammalian

Promoter

CMV

Available Since

Nov. 12, 2020

Availability

Academic Institutions and Nonprofits only

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pGG-cTP-TurboID

Plasmid

#209394

Purpose

Transiently expressing TurboID fused with a chloroplast transit peptide (cTP) in planta

Depositor

Rosa Lozano-Durán

Article

Tan et al J Integr Plant Biol. 2024 Jan 31. doi: 1

Insert

cTP-3XHA-TurboID

Expression

Plant

Promoter

Cauliflower mosaic virus (CaMV) 35S promoter

Available Since

Jan. 5, 2024

Availability

Academic Institutions and Nonprofits only

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pAAV-EF1a-FLEX-TVA-mCherry

Plasmid

#38044

Depositor

Naoshige Uchida

Article

Watabe-Uchida et al Neuron. 2012 Jun 7;74(5):858-73.

Insert

Avian sarcoma and leukemia virus receptor 950 (CD320 Synthetic, quail)

Use

AAV and Cre/Lox

Tags

mCherry

Expression

Mammalian

Promoter

EF1a

Available Since

Aug. 15, 2012

Availability

Academic Institutions and Nonprofits only

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pLEX-FLAG-RHEB-IRES-Puro

Plasmid

#120576

Purpose

Expresses Flag-RHEB fusion protein in mammalian cells & for virus production

Depositor

Paul Khavari

Article

Kovalski et al Mol Cell. 2019 Jan 4. pii: S1097-2765(18

Insert

RHEB (RHEB Human)

Use

Lentiviral

Tags

Flag

Expression

Mammalian

Promoter

CMV

Available Since

Jan. 29, 2019

Availability

Academic Institutions and Nonprofits only

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pET28-SaCas9

Plasmid

#178901

Purpose

SaCas9 (Cas9 from Staphylococcus aureus)

Depositor

Hendrik Milting

Article

Flottmann et al STAR Protoc. 2022 Apr 5;3(2):101276. doi

Insert

SaCas9

Tags

His6-Tag, bipartite nuclear localization signal f…

Expression

Bacterial

Available Since

July 8, 2022

Availability

Academic Institutions and Nonprofits only

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pGG-GFP-PM-TurboID

Plasmid

#209402

Purpose

Transiently expressing and visualizing the subcellular localization of TurboID fused with a plasma menbrane targeting peptide in planta

Depositor

Rosa Lozano-Durán

Article

Tan et al J Integr Plant Biol. 2024 Jan 31. doi: 1

Insert

cTP-3XHA-TurboID

Tags

GFP

Expression

Plant

Promoter

Cauliflower mosaic virus (CaMV) 35S promoter

Available Since

Jan. 5, 2024

Availability

Academic Institutions and Nonprofits only

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AAV-FLEX-rev-ChR2-tdtomato

Plasmid

#18917

Purpose

Cre-dependent viral expression of ChR2-tdTomato for neuronal excitation

Depositor

Scott Sternson

Article

Atasoy et al J Neurosci. 2008 Jul 9. 28(28):7025-30.

Insert

Channelrhodopsin 2-tdtomato

Use

AAV and Cre/Lox; Adeno-associated virus

Tags

tdtomato

Promoter

CAG

Available Since

Feb. 9, 2009

Availability

Academic Institutions and Nonprofits only

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