4,082 results

1. lenti sgRNA(MS2)_puro backbone

   Plasmid

   #73795

   Purpose

   3rd generation lenti sgRNA cloning backbone with MS2 loops at tetraloop and stemloop 2 and EF1a-puro resistance marker. Contains BsmBI sites for insertion of spacer sequences.

   Depositor

   Feng Zhang

   Article

   Konermann et al Nature. 2014 Dec 10. doi: 10.1038/nature

   Has Service

   Cloning Grade DNA

   Type

   Empty backbone

   Use

   CRISPR and Lentiviral

   Tags

   Expression

   Mammalian

   Mutation

   Promoter

   U6 and EF1A

   Available since

   March 15, 2016

   Availability

   Academic Institutions and Nonprofits only

   

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2. pKLV2-U6gRNA5(BbsI)-PGKpuro2AmCherry-W

   Plasmid

   #67977

   Purpose

   CRISPR gRNA expression vector with an improved scaffold and puro/mCherry markers

   Depositor

   Kosuke Yusa

   Article

   Tzelepis et al Cell Rep. 2016 Oct 18;17(4):1193-1205. d

   Insert

   U6gRNA cassette, PGKpuro2AmCherry cassette, WPRE
   

   Use

   Lentiviral

   Tags

   Expression

   Mutation

   Deleted BbsI site within WPRE

   Promoter

   human U6 promoter, mouse PGK promoter

   Available since

   Sept. 23, 2016

   Availability

   Academic Institutions and Nonprofits only

   

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3. GPD-roGFP

   Plasmid

   #49436

   Purpose

   Expresses thiol redox-sensitive ratiometric sensor roGFP in the mitochondrial intermembrane space of mammalian cells

   Depositor

   Paul Schumacker

   Article

   Waypa et al Circ Res. 2010 Feb 19;106(3):526-35. doi

   Insert

   GPD-roGFP2
   

   Use

   Adenoviral

   Tags

   Glycerol phosphate dehydrogenase, 5' to roGF…

   Expression

   Mammalian

   Mutation

   C48S, Q80R, S147C, and Q204C in Clontech's G…

   Promoter

   CMV

   Available since

   Dec. 10, 2013

   Availability

   Academic Institutions and Nonprofits only

   

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4. pLentiCRISPR-EGFP

   Plasmid

   #75159

   Purpose

   Derived from LentiCRISPR v1 but expresses EGFP instead of puromycin resistance

   Depositor

   Beat Bornhauser

   Article

   McComb et al Sci Transl Med. 2016 May 18;8(339):339ra

   Type

   Empty backbone

   Use

   CRISPR and Lentiviral

   Tags

   EGFP

   Expression

   Mammalian

   Mutation

   Promoter

   Available since

   May 17, 2016

   Availability

   Academic Institutions and Nonprofits only

   

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5. myc-Raptor

   Plasmid

   #1859

   Depositor

   David Sabatini

   Article

   Sarbassov et al Curr Biol 2004 Jul 27;14(14):1296-302.

   Insert

   Raptor (RPTOR Human)
   

   Use

   Tags

   Myc

   Expression

   Mammalian

   Mutation

   Promoter

   Available since

   June 20, 2005

   Availability

   Academic Institutions and Nonprofits only

   

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6. pYESir2-puro

   Plasmid

   #1769

   Depositor

   Bob Weinberg

   Article

   Vaziri et al Cell 2001 Oct 19;107(2):149-59.

   Insert

   Sir2 (SIRT1 Human)
   

   Use

   Retroviral

   Tags

   Expression

   Mammalian

   Mutation

   Promoter

   Available since

   June 3, 2005

   Availability

   Academic Institutions and Nonprofits only

   

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7. pDESTmycDICER

   Plasmid

   #19873

   Depositor

   Thomas Tuschl

   Article

   Landthaler et al RNA. 2008 Oct 31. ():.

   Insert

   dicer 1, ribonuclease type III (DICER1 Human)
   

   Use

   Tags

   His and Myc

   Expression

   Mammalian

   Mutation

   Promoter

   Available since

   Dec. 31, 2008

   Availability

   Academic Institutions and Nonprofits only

   

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8. Flag-HA-OTUD5

   Plasmid

   #22610

   Depositor

   Wade Harper

   Article

   Sowa et al Cell. 2009 Jul 23. 138(2):389-403.

   Insert

   OTUD5 (OTUD5 Human)
   

   Use

   Retroviral

   Tags

   FLAG and HA

   Expression

   Mammalian

   Mutation

   Promoter

   CMV/TO

   Available since

   Nov. 13, 2009

   Availability

   Academic Institutions and Nonprofits only

   

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9. pCW57-GFP-2A-MCS

   Plasmid

   #71783

   Purpose

   All-in-one doxycycline inducible lentiviral vector for expression of one gene in combination with turbo GFP using the P2A self-cleaving peptide.

   Depositor

   Adam Karpf

   Article

   Barger et al Cancers (Basel). 2019 Feb 21;11(2). pii:

   Has Service

   Cloning Grade DNA

   Type

   Empty backbone

   Use

   Lentiviral; Doxycycline inducible; p2a self cleav…

   Tags

   Expression

   Mammalian

   Mutation

   Promoter

   Available since

   Feb. 22, 2016

   Availability

   Academic Institutions and Nonprofits only

   

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10. pCAX APP 695

    Plasmid

    #30137

    Depositor

    Tracy Young-Pearse, Dennis Selkoe

    Article

    Young-Pearse et al J Neurosci. 2007 Dec 26. 27(52):14459-69

    Insert

    Amyloid Precursor Protein (APP Human)
    

    Use

    Tags

    Expression

    Mammalian

    Mutation

    wild type splice variant 695

    Promoter

    Available since

    Aug. 15, 2011

    Availability

    Academic Institutions and Nonprofits only

    

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