178,137 results

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pRSFDuet-1(nsp8-nsp7)(nsp12)

Plasmid

#165451

Purpose

Expresses the RNA Dependent RNA polymerase complex of SARS-CoV-2 in Escherichia Coli

Depositor

Marc Delarue

Publication

Madru et al PLoS One. 2021 Apr 29;16(4):e0250610. do

Inserts

nsp12
nsp8
nsp7

Tags

14Histidine-TEV

Expression

Bacterial

Promoter

T7

Available since

May 25, 2021

Availability

Academic Institutions and Nonprofits only

Cited by

7 Articles

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Nb-gp41-GFP (MoonTag-Nb-GFP)

Plasmid

#128602

Purpose

Expresses nanobody-2H10 fused to super folded GFP (sfGFP) in mammalian cells. It recognizes gp41 peptide array (MoonTag peptide array).

Depositor

Marvin Tanenbaum

Publication

Boersma et al Cell. 2019 Jun 5. pii: S0092-8674(19)304

Insert

Nanobody_gp41

Use

Lentiviral

Tags

GB1 and sfGFP

Promoter

SFFV

Available since

Oct. 30, 2019

Availability

Academic Institutions and Nonprofits only

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T444T

Plasmid

#113081

Purpose

Enhanced RNAi vector for C. elegans. Contains two T7 polymerase termination sequences adjacent to the T7 promoter sites.

Depositor

Tibor Vellai

Publication

Sturm et al Nucleic Acids Res. 2018 Jun 19. pii: 504

Type

Empty backbone

Use

RNAi

Expression

Bacterial

Available since

Sept. 7, 2018

Availability

Academic Institutions and Nonprofits only

Cited by

8 Articles

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pmGFP-P2A-K(AAA)20-P2A-RFP

Plasmid

#105688

Purpose

Double fluorescent stalling reporter K(AAA)20

Depositor

Ramanujan Hegde

Publication

Juszkiewicz et al Mol Cell. 2017 Feb 16;65(4):743-750.e4.

Has service

50 µg Transfection-Ready DNA and 100 µg Transfection-Ready DNA

Inserts

P2A-3xFLAG-VHPbeta
K(AAA)20
P2A-mCherry

Expression

Mammalian

Promoter

CMV (from vector)

Available since

Feb. 6, 2018

Availability

Academic Institutions and Nonprofits only

Cited by

8 Articles

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psfTn5

Plasmid

#79107

Purpose

bacterial expression of hyperactive Tn5 transposase carrying E54K and L372P mutations

Depositor

Sten Linnarsson

Insert

transposase 5

Tags

His

Expression

Bacterial

Mutation

E54K and L372P

Available since

March 17, 2017

Availability

Academic Institutions and Nonprofits only

Cited by

3 Articles

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pcDNA3.1 phiC31

Plasmid

#68310

Purpose

In vitro transcription vector to generate phiC31 mRNA (i.e. for zebrafish injection).

Depositor

Konrad Basler

Publication

Bischof et al Proc Natl Acad Sci U S A. 2007 Feb 27;10

Insert

phiC31

Use

Rna transcription

Promoter

CMV

Available since

Aug. 27, 2015

Availability

Academic Institutions and Nonprofits only

Cited by

4 Articles

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MSP469

Plasmid

#65771

Purpose

Human expression vector for SpCas9 VQR variant: CMV-T7-humanSpCas9(D1135V/R1335Q/T1337R)-NLS-3xFLAG (VQR variant)

Depositor

Keith Joung

Publication

Kleinstiver et al Nature. 2015 Jun 22. doi: 10.1038/nature

Has service

50 µg Transfection-Ready DNA and 100 µg Transfection-Ready DNA

Insert

mammalian codon-optimized Streptococcus pyogenes Cas9 (D1135V/R1335Q/T1337R)-NLS-3XFlag

Use

CRISPR

Tags

3x FLAG and NLS

Expression

Mammalian

Mutation

D1135V, R1335Q, and T1337R mutations in Cas9

Promoter

CMV & T7

Available since

June 22, 2015

Availability

Academic Institutions and Nonprofits only

Cited by

6 Articles

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pSBbi-Bla

Plasmid

#60526

Purpose

SB-transposon with constitutive bi-directional promoter, one side: SfiI cloning site for GOI (contains filler DNA), other side: blasticidin resistance gene

Depositor

Eric Kowarz

Publication

Kowarz et al Biotechnol J. 2015 Feb 4. doi: 10.1002/b

Type

Empty backbone

Use

Transposon

Expression

Mammalian

Promoter

EF1a/RPBSA

Available since

Feb. 11, 2015

Availability

Academic Institutions and Nonprofits only

Cited by

17 Articles

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EGFP-Tubulin-6

Plasmid

#56450

Purpose

Localization: Microtubules, Excitation: 488, Emission: 507

Depositor

Michael Davidson

Publication

Rizzo et al Cold Spring Harb Protoc. 2009 Dec;2009(1

Has service

50 µg Transfection-Ready DNA and 100 µg Transfection-Ready DNA

Insert

Tubulin (TUBA1B Human)

Tags

EGFP

Expression

Mammalian

Promoter

CMV

Available since

Jan. 13, 2015

Availability

Industry, Academic Institutions, and Nonprofits

Cited by

11 Articles

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pMLS-SV40-EGFP

Plasmid

#46919

Purpose

Target EGFP gene that is stably integrated into HEK293 genome

Depositor

Stanley Qi, Jonathan Weissman

Publication

Gilbert et al Cell. 2013 Jul 9. pii: S0092-8674(13)008

Insert

GFP

Use

CRISPR and Retroviral

Expression

Mammalian

Promoter

SV40

Available since

Oct. 1, 2013

Availability

Academic Institutions and Nonprofits only

Cited by

5 Articles

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pTriExRhoA2G

Plasmid

#40176

Depositor

Olivier Pertz

Publication

Fritz et al Sci Signal. 2013 Jul 23;6(285):rs12. doi

Has service

50 µg Transfection-Ready DNA and 100 µg Transfection-Ready DNA

Insert

RhoA Biosensor

Tags

His-Myc

Expression

Bacterial, Insect, and Mamm…

Promoter

CMV

Available since

July 5, 2013

Availability

Academic Institutions and Nonprofits only

Cited by

14 Articles

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hRIP3 GFP wt

Plasmid

#41387

Depositor

Francis Chan

Publication

Cho et al Cell. 2009 Jun 12;137(6):1112-23.

Has service

50 µg Transfection-Ready DNA and 100 µg Transfection-Ready DNA

Insert

RIP3 (RIPK3 Human)

Tags

GFP

Expression

Mammalian

Promoter

CMV

Available since

Dec. 4, 2012

Availability

Academic Institutions and Nonprofits only

Cited by

9 Articles

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FUW-TetO-Sox9

Plasmid

#41080

Depositor

Rudolf Jaenisch

Publication

Buganim et al Cell Stem Cell. 2012 Sep 7;11(3):373-86.

Insert

Sox9 (Sox9 Mouse)

Use

Lentiviral

Expression

Mammalian

Promoter

TetO + minimal CMV

Available since

Nov. 2, 2012

Availability

Academic Institutions and Nonprofits only

Cited by

5 Articles

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pET9d-hnRNP-A1

Plasmid

#23026

Depositor

Douglas Black

Insert

hnRNPA1 (HNRNPA1 Human)

Expression

Bacterial

Promoter

T7

Available since

Oct. 26, 2012

Availability

Academic Institutions and Nonprofits only

Cited by

5 Articles

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-1748 human cyclin D1 promoter pGL3Basic

Plasmid

#32726

Depositor

Frank McCormick

Publication

Tetsu et al Nature. 1999 Apr 1;398(6726):422-6.

Has service

50 µg Transfection-Ready DNA and 100 µg Transfection-Ready DNA

Insert

CCND1 promoter (CCND1 Human)

Use

Luciferase; Reporter gene

Promoter

-1748CCND1 promoter

Available since

Jan. 27, 2012

Availability

Academic Institutions and Nonprofits only

Cited by

9 Articles

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pN1-Lck-GCaMP3

Plasmid

#26974

Depositor

Baljit Khakh

Publication

Shigetomi et al Neuron Glia Biol. 2010 Dec 16:1-9. [Epub

Has service

50 µg Transfection-Ready DNA and 100 µg Transfection-Ready DNA

Insert

Lck-GCaMP3

Tags

His and Xpress

Expression

Mammalian

Mutation

Plasmid contains N-terminal 26 amino acid membran…

Available since

Jan. 27, 2011

Availability

Academic Institutions and Nonprofits only

Cited by

8 Articles

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pcDNA3.1 EYFP H148Q/I152L

Plasmid

#25872

Depositor

Peter Haggie

Publication

Galietta et al FEBS Lett. 2001 Jun 22. 499(3):220-4.

Has service

50 µg Transfection-Ready DNA and 100 µg Transfection-Ready DNA

Insert

EYFP H148Q/I152L

Expression

Mammalian

Mutation

EYFP from pEYFP (Clontech) with H148Q and I152L m…

Available since

Aug. 16, 2010

Availability

Academic Institutions and Nonprofits only

Cited by

4 Articles

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pLKO-PTEN-shRNA-3001

Plasmid

#25639

Depositor

Todd Waldman

Publication

Kim et al Mol Cell Biol. 2007 Jan . 27(2):662-77.

Insert

PTEN-shRNA-3001 (PTEN Human)

Use

Lentiviral and RNAi

Expression

Mammalian

Available since

June 21, 2010

Availability

Academic Institutions and Nonprofits only

Cited by

4 Articles

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Flag-HA-USP1

Plasmid

#22596

Depositor

Wade Harper

Publication

Sowa et al Cell. 2009 Jul 23. 138(2):389-403.

Insert

USP1 (USP1 Human)

Use

Retroviral

Tags

FLAG and HA

Expression

Mammalian

Mutation

None

Available since

Nov. 13, 2009

Availability

Academic Institutions and Nonprofits only

Cited by

2 Articles

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HA-EglN1-pcDNA3

Plasmid

#18963

Depositor

William Kaelin

Publication

Ivan et al Proc Natl Acad Sci U S A. 2002 Oct 15. 9

Has service

50 µg Transfection-Ready DNA and 100 µg Transfection-Ready DNA

Insert

Egg laying Nine - 1 (EGLN1 Human)

Tags

HA-tag

Expression

Mammalian

Mutation

wt cDNA

Available since

Aug. 20, 2008

Availability

Academic Institutions and Nonprofits only

Cited by

8 Articles

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