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We narrowed to 18,548 results for: Mut

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Showing: 3081 - 3100 of 18548 results

  2. pET28a-ybbr-ELP-ddFLN4-XMod-Doc-HIS (BMA-KO)

    Plasmid
    #153440
    Purpose
    E. coli expression of Rc. XDocB binding mode A knock-out construct containing ybbr tag, ELP linker and ddFLN4 fingerprint domain. This construct was designed for AFM measurements.
    Depositor
    Michael Nash
    Article
    Liu et al Nat Commun. 2020 Aug 28;11(1):4321. doi:
    Insert
    Rc. XDocB binding mode A knock-out mutant
    Tags
    3xELP linker, 6xHis, ddFLN4 fingerprint domain, a…
    Expression
    Bacterial
    Mutation
    Arginine 191 and leucine 195 on XDocB were mutate…
    Promoter
    T7 promoter
    Available Since
    July 22, 2020
    Availability
    Academic Institutions and Nonprofits only
    153440-plasmid-map-sequence-id-306440

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  4. pb-NES-ZapCV5 (cpV143)

    Plasmid
    #112061
    Purpose
    Genetically-encoded Cyan-Yellow Zinc FRET sensor. Localizes to the cytosol. Low affinity to free zinc (Kd ~ 300nM, n = 0.55) due to mutation of the zinc binding Cys residues to His.
    Depositor
    Amy Palmer
    Article
    Fiedler et al Anal Chem. 2017 Jan 3;89(1):711-719. doi
    Insert
    NES-ZapCV5
    Tags
    Nuclear Export Signal (NES) derived from HIV-1
    Expression
    Mammalian
    Mutation
    ECFP gene is truncated 36 bp at 3' end. Venu…
    Promoter
    CBA
    Available Since
    July 9, 2018
    Availability
    Academic Institutions and Nonprofits only

  5. pLuc-Optop38i-dsm

    Plasmid
    #89748
    Purpose
    Expression of light-regulated p38 inhibitor, firefly luciferase-fused, dark-state mutant photosensor (AsLov2Ja.C450A), inhibitor MK3BD3-13F
    Depositor
    Michael Courtney
    Article
    Melero-Fernandez de Mera et al Nat Commun. 2017 May 12;8:15017. doi: 10
    Insert
    Optop38i dark-state mutant
    Use
    Luciferase
    Tags
    Firefly luciferase
    Expression
    Mammalian
    Mutation
    C450A mutation of AsLov2Jalpha
    Promoter
    CMV
    Available Since
    Aug. 29, 2017
    Availability
    Academic Institutions and Nonprofits only
    89748-plasmid-map-sequence-id-192493

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  8. pLgw EcoDam-V5-NKX2-5Y191C

    Plasmid
    #98598
    Purpose
    Mammalian DamID lentiviral vector for fusing NKX2-5 Y191C with Dam-V5 using Gateway cloning
    Depositor
    Richard Harvey
    Article
    Bouveret et al Elife. 2015 Jul 6;4. doi: 10.7554/eLife.
    Insert
    NKX2-5 (Nkx2-5 Mouse)
    Use
    Lentiviral; Damid
    Tags
    Dam (DNA adenine methyltransferase) and V5
    Expression
    Mammalian
    Mutation
    Y191C point mutant
    Promoter
    Heat Shock Minimal Promoter
    Available Since
    Aug. 7, 2017
    Availability
    Academic Institutions and Nonprofits only
    98598-plasmid-map-sequence-id-190106

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  9. pLgw NKX2-5Y191C-V5-EcoDam

    Plasmid
    #98599
    Purpose
    Mammalian DamID lentiviral vector for fusing NKX2-5 Y191C with Dam-V5 using Gateway cloning
    Depositor
    Richard Harvey
    Article
    Bouveret et al Elife. 2015 Jul 6;4. doi: 10.7554/eLife.
    Insert
    NKX2-5 (Nkx2-5 Mouse)
    Use
    Lentiviral; Damid
    Tags
    V5 and Dam (DNA adenine methyltransferase)
    Expression
    Mammalian
    Mutation
    Y191C point mutant
    Promoter
    Heat Shock Minimal Promoter
    Available Since
    July 26, 2017
    Availability
    Academic Institutions and Nonprofits only
    98599-plasmid-map-sequence-id-189181

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  11. pIS1 mHK1

    Plasmid
    #60795
    Purpose
    Luciferase reporter to measure miR-155 mediated differential repression. Contains HK1 3' UTR and mutated miR-155 sites
    Depositor
    David Bartel
    Article
    Nam et al Mol Cell. 2014 Mar 20;53(6):1031-43. doi
    Insert
    HK1 3'UTR and mutated miR-155 binding site (HK1 Human)
    Use
    Luciferase
    Expression
    Mammalian
    Mutation
    mutated miR-155 binding site
    Promoter
    thymidine kinase (HSV-TK promoter)
    Available Since
    Oct. 20, 2014
    Availability
    Academic Institutions and Nonprofits only
    60795-plasmid-map-sequence-id-97806

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  14. AAV-CAG-FLPX-rc [ChrimsonR-tdTomato]

    Plasmid
    #130909
    Purpose
    AAV-mediated expression of ChrimsonR-tdTomato under the CAG promoter in frt/reversed (Flp-dependent) manner. tdTomato has codons varied to reduce recombination.
    Depositor
    Edward Boyden
    Article
    Klapoetke et al Nat Methods. 2014 Mar;11(3):338-46. doi:
    Has Service
    AAV8
    Insert
    ChrimsonR-tdTomato
    Use
    AAV
    Tags
    tdTomato (codon diversified version)
    Expression
    Mammalian
    Mutation
    Chrimson K176R mutant
    Promoter
    CAG
    Available Since
    Sept. 25, 2019
    Availability
    Academic Institutions and Nonprofits only
    130909-plasmid-map-sequence-id-257665

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  15. LV-AR-KKKAAA

    Plasmid
    #171219
    Purpose
    Lenti-viral expression of human androgen receptor DNA-binding mutant: K630A-K632A-K633A, mutations at the boundary between AR DNA-binding domain and hinge domain.
    Depositor
    Steven Balk
    Article
    Guo et al Nat Commun. 2021 Dec 15;12(1):7308. doi:
    Insert
    AR-KKKAAA (AR Human)
    Use
    Lentiviral
    Tags
    Flag
    Expression
    Mammalian
    Mutation
    AR-K630A-K632A-K633A
    Promoter
    EF-1a
    Available Since
    Sept. 14, 2021
    Availability
    Academic Institutions and Nonprofits only
    171219-plasmid-map-sequence-id-342535

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  17. pAAV-Syn-FLEX-rc[ChrimsonR-tdTomato]

    Plasmid
    #62723
    Purpose
    AAV-mediated expression of ChrimsonR-tdTomato under the Syn promoter, in floxed/reversed (Cre-dependent) manner. Using bGHpA signal. TdTomato is a codon diversified version.
    Depositor
    Edward Boyden
    Article
    Klapoetke et al Nat Methods. 2014 Mar;11(3):338-46. doi:
    Has Service
    AAV1 and AAV5
    Insert
    ChrimsonR-tdTomato
    Use
    AAV
    Tags
    tdTomato
    Expression
    Mammalian
    Mutation
    Chrimson K176R mutant
    Promoter
    human synapsin promoter
    Available Since
    April 30, 2015
    Availability
    Academic Institutions and Nonprofits only
    62723-plasmid-map-sequence-id-166279

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  19. pC0054-CMV-dPspCas13b-longlinker-ADAR2DD(E488Q/T375G)

    Plasmid
    #103870
    Purpose
    High specificity version of dPspCas13b-ADAR2DD(E488Q) fusions. T375G mutation in the ADAR deaminase domain confers increased specificity with slightly reduced activity.
    Depositor
    Feng Zhang
    Article
    Cox et al Science. 2017 Oct 25. pii: eaaq0180. doi
    Inserts
    PspCas13b
    huADAR2DD (ADARB1 Human)
    Use
    CRISPR
    Tags
    GSGGGGS and HIV NES
    Expression
    Mammalian
    Mutation
    Changed histidne 133 to alanine and histidine 105…
    Available Since
    Dec. 13, 2017
    Availability
    Academic Institutions and Nonprofits only
    103870-plasmid-map-sequence-id-199178

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Showing: 3081 - 3100 of 18548 results