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pspCas9-Exo-tetra-com-hCLCN5-sp-g1

Plasmid

#176238

Purpose

Plasmid for expressing a fusion protein of spCas9 and the 5’ and 3’ exonuclease domain of POLI, and sgRNA targeting human CLCN5 gene.

Depositor

Baisong Lu

Article

Yoo et al Nucleic Acids Res. 2022 Mar 22. pii: 655

Insert

spCas9 and polymerase exo domain fusion (CLCN5 Human)

Use

Tags

Expression

Bacterial

Mutation

Promoter

Available since

March 30, 2022

Availability

Academic Institutions and Nonprofits only

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pGEX4T3-GST-NQO1(Y128F)

Plasmid

#177141

Purpose

Bacterial vector for expression of an N-terminal GST fusion of NQO1(Y128F) with a TEV protease site located between the GST tag and NQO1(Y128F)

Depositor

Robert Sobol

Article

Fang et al Nucleic Acids Res. 2019 Jul 9;47(12):626

Insert

NQO1 (NQO1 Human)

Use

Tags

GST

Expression

Bacterial

Mutation

Promoter

Tac

Available since

Dec. 16, 2021

Availability

Academic Institutions and Nonprofits only

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pGEX4T3-GST-PolB(K206A/K244A/L301R/V303R/V306R)

Plasmid

#177138

Purpose

Bacterial vector for expression of an N-terminal GST fusion of PolB(K206A/K244A/L301R/V303R/V306R) with a TEV protease site located between the GST tag and PolB(K206A/K244A/L301R/V303R/V306R)

Depositor

Robert Sobol

Article

Fang et al Nucleic Acids Res. 2019 Jul 9;47(12):626

Insert

PolB (POLB Human)

Use

Tags

GST

Expression

Bacterial

Mutation

Promoter

Tac

Available since

Dec. 16, 2021

Availability

Academic Institutions and Nonprofits only

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pGEX4T3-GST-PolB(L301R/V303R/V306R)

Plasmid

#177137

Purpose

Bacterial vector for expression of an N-terminal GST fusion of PolB(L301R/V303R/V306R) with a TEV protease site located between the GST tag and PolB(L301R/V303R/V306R)

Depositor

Robert Sobol

Article

Fang et al Nucleic Acids Res. 2019 Jul 9;47(12):626

Insert

PolB (POLB Human)

Use

Tags

GST

Expression

Bacterial

Mutation

Promoter

Tac

Available since

Dec. 16, 2021

Availability

Academic Institutions and Nonprofits only

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pGEX4T3-GST-PolB(K206A/K244A/T304I)

Plasmid

#177134

Purpose

Bacterial vector for expression of an N-terminal GST fusion of PolB(K206A/K244A/T304I) with a TEV protease site located between the GST tag and PolB(K206A/K244A/T304I)

Depositor

Robert Sobol

Article

Fang et al Nucleic Acids Res. 2019 Jul 9;47(12):626

Insert

PolB (POLB Human)

Use

Tags

GST

Expression

Bacterial

Mutation

Promoter

Tac

Available since

Dec. 16, 2021

Availability

Academic Institutions and Nonprofits only

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PEA1-Nuclease

Plasmid

#176528

Purpose

Delivers all prime editing nuclease components in a single plasmid

Depositor

Paul Thomas

Article

Adikusuma et al Nucleic Acids Res. 2021 Sep 17. pii: 637

Insert

CbH-Cas9-RT, hU6-pegRNA (Bbs1 gate), hU6-sgRNA (Bbs1 gate)

Use

CRISPR

Tags

Expression

Mammalian

Mutation

GC to CA point mutation in SpCas9 to restore nucl…

Promoter

CbH for Cas9, hU6 for gRNAs

Available since

Dec. 16, 2021

Availability

Academic Institutions and Nonprofits only

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pRD400

Plasmid

#163107

Purpose

Expression of eYFP_H-NSdbd in eukaryotes for visualisation of nuclear DNA

Depositor

Remus Dame

Article

Rashid et al Nucleic Acids Res. 2021 Nov 4. pii: 6414

Insert

NLS_eYFP_H-NSdbd_NLS

Use

Eukaryotic expression

Tags

Expression

Mammalian

Mutation

Promoter

CMV promoter; T7 promoter

Available since

Oct. 29, 2021

Availability

Academic Institutions and Nonprofits only

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pRD403

Plasmid

#163108

Purpose

Expression of mScarlet-I_H-NSdbd in Escherichia coli (and, potentially, other bacteria)

Depositor

Remus Dame

Article

Rashid et al Nucleic Acids Res. 2021 Nov 4. pii: 6414

Insert

mScarlet-I_H-NSdbd

Use

Tags

Expression

Bacterial

Mutation

Promoter

araBAD promoter

Available since

Oct. 29, 2021

Availability

Academic Institutions and Nonprofits only

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pRD128

Plasmid

#163097

Purpose

Expression of mTurquoise2_H-NSdbd in Escherichia coli (and, potentially, other bacteria)

Depositor

Remus Dame

Article

Rashid et al Nucleic Acids Res. 2021 Nov 4. pii: 6414

Insert

mTurquoise2_H-NSdbd

Use

Tags

Expression

Bacterial

Mutation

Promoter

araBAD promoter

Available since

Oct. 29, 2021

Availability

Academic Institutions and Nonprofits only

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pRD129

Plasmid

#163098

Purpose

Expression of mEos3.2_H-NSdbd in Escherichia coli (and, potentially, other bacteria)

Depositor

Remus Dame

Article

Rashid et al Nucleic Acids Res. 2021 Nov 4. pii: 6414

Insert

mEos3.2_H-NSdbd

Use

Tags

Expression

Bacterial

Mutation

Promoter

araBAD promoter

Available since

Oct. 29, 2021

Availability

Academic Institutions and Nonprofits only

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pRD188

Plasmid

#163099

Purpose

Expression of mEos3.2_H-NSdbd in eukaryotes for visualisation of nuclear DNA

Depositor

Remus Dame

Article

Rashid et al Nucleic Acids Res. 2021 Nov 4. pii: 6414

Insert

NLS_mEos3.2_H-NSdbd_NLS

Use

Eukaryotic expression

Tags

Expression

Mammalian

Mutation

Promoter

CMV promoter; T7 promoter

Available since

Oct. 29, 2021

Availability

Academic Institutions and Nonprofits only

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pRD198

Plasmid

#163101

Purpose

Expression of mEGFP_H-NSdbd in Escherichia coli (and, potentially, other bacteria)

Depositor

Remus Dame

Article

Rashid et al Nucleic Acids Res. 2021 Nov 4. pii: 6414

Insert

mEGFP_H-NSdbd

Use

Tags

Expression

Bacterial

Mutation

Promoter

araBAD promoter

Available since

Oct. 29, 2021

Availability

Academic Institutions and Nonprofits only

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pRD396

Plasmid

#163103

Purpose

Expression of eYFP_H-NSdbd in Escherichia coli (and, potentially, other bacteria)

Depositor

Remus Dame

Article

Rashid et al Nucleic Acids Res. 2021 Nov 4. pii: 6414

Insert

eYFP_H-NSdbd

Use

Tags

Expression

Bacterial

Mutation

Promoter

araBAD promoter

Available since

Oct. 29, 2021

Availability

Academic Institutions and Nonprofits only

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pRD437

Plasmid

#168463

Purpose

For the insertion pf NLS-mEos3.2-H-NSdbd-NLS into the chromosome of a eukaryotic cell line carrying the Flp-IN/T-Rex system

Depositor

Remus Dame

Article

Rashid et al Nucleic Acids Res. 2021 Nov 4. pii: 6414

Insert

NLS-mEos3.2-H-NSdbd-NLS

Use

Tags

Expression

Mammalian

Mutation

Promoter

CMV promoter

Available since

Oct. 29, 2021

Availability

Academic Institutions and Nonprofits only

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pRD438

Plasmid

#168464

Purpose

For the insertion pf NLS-mEos3.2-NLS into the chromosome of a eukaryotic cell line carrying the Flp-IN/T-Rex system

Depositor

Remus Dame

Article

Rashid et al Nucleic Acids Res. 2021 Nov 4. pii: 6414

Insert

NLS-mEos3.2-NLS

Use

Tags

Expression

Mammalian

Mutation

Promoter

CMV promoter

Available since

Oct. 29, 2021

Availability

Academic Institutions and Nonprofits only

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pRD440

Plasmid

#168466

Purpose

For the insertion pf NLS-mCherry-H-NSdbd-NLS into the chromosome of a eukaryotic cell line carrying the Flp-IN/T-Rex system

Depositor

Remus Dame

Article

Rashid et al Nucleic Acids Res. 2021 Nov 4. pii: 6414

Insert

NLS-mCherry-H-NSdbd-NLS

Use

Tags

Expression

Mammalian

Mutation

Promoter

CMV promoter

Available since

Oct. 29, 2021

Availability

Academic Institutions and Nonprofits only

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pRD441

Plasmid

#168467

Purpose

For the insertion pf NLS-mEGFP-H-NSdbd-NLS into the chromosome of a eukaryotic cell line carrying the Flp-IN/T-Rex system

Depositor

Remus Dame

Article

Rashid et al Nucleic Acids Res. 2021 Nov 4. pii: 6414

Insert

NLS-mEGFP-H-NSdbd-NLS

Use

Tags

Expression

Mammalian

Mutation

Promoter

CMV promoter

Available since

Oct. 29, 2021

Availability

Academic Institutions and Nonprofits only

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pRD442

Plasmid

#168468

Purpose

For the insertion of NLS-eYFP-H-NSdbd-NLS into the chromosome of a eukaryotic cell line carrying the Flp-IN/T-Rex system

Depositor

Remus Dame

Article

Rashid et al Nucleic Acids Res. 2021 Nov 4. pii: 6414

Insert

NLS-eYFP-H-NSdbd-NLS

Use

Tags

Expression

Mammalian

Mutation

Promoter

CMV promoter

Available since

Oct. 29, 2021

Availability

Academic Institutions and Nonprofits only

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pRD444

Plasmid

#168470

Purpose

For the insertion of NLS-mNeonGreen-H-NSdbd-NLS into the chromosome of a eukaryotic cell line carrying the Flp-IN/T-Rex system

Depositor

Remus Dame

Article

Rashid et al Nucleic Acids Res. 2021 Nov 4. pii: 6414

Insert

NLS-mNeonGreen-H-NSdbd-NLS

Use

Tags

Expression

Mammalian

Mutation

Promoter

CMV promoter

Available since

Oct. 29, 2021

Availability

Academic Institutions and Nonprofits only

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pUPD2 Register 1/3 Tnos:YFP:5'UTR (GB1507)

Plasmid

#160583

Purpose

Part 1/3 for assembly of site-specific recombination based Registers. Composed of the reversed sequences of the TNos terminator,YFP CDS and 5' UTR of 35S promoter (TNos:YFP:5'UTR)

Depositor

Diego Orzaez

Article

Bernabe-Orts et al Nucleic Acids Res. 2020 Apr 6;48(6):3379

Insert

YFP

Use

Synthetic Biology

Tags

Expression

Mutation

BsaI and BsmBI sites removed

Promoter

Available since

Aug. 2, 2021

Availability

Academic Institutions and Nonprofits only

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