We narrowed to 16,281 results for: She

1. pAAV-CMV-FLEX-SaCas9-U6-sgVamp1

   Plasmid

   #159919

   Purpose

   Mutagenesis of Vamp1

   Depositor

   Larry Zweifel

   Insert

   Vamp1 (Vamp1 Mouse)
   

   Use

   AAV, CRISPR, and Mouse Targeting

   Tags

   Expression

   Mutation

   Promoter

   Available since

   Nov. 16, 2020

   Availability

   Academic Institutions and Nonprofits only

   

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2. pAAV-CMV-FLEX-SaCas9-U6-sgNtn1

   Plasmid

   #159907

   Purpose

   Mutagenesis of Netrin1

   Depositor

   Larry Zweifel

   Insert

   Netrin1 (Ntn1 Mouse)
   

   Use

   AAV, CRISPR, and Mouse Targeting

   Tags

   Expression

   Mutation

   Promoter

   Available since

   Nov. 16, 2020

   Availability

   Academic Institutions and Nonprofits only

   

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3. pLL1272

   Plasmid

   #129287

   Purpose

   Expression plasmid with pLL1270 as backbone and alpha-Xylp_1211 and alpha-L-Araf_1120 as inserts

   Depositor

   Lee Lynd

   Inserts

   alpha-Xylp_1211 (Ga0256695_ 1211)
   alpha-L-Araf_1120
   

   Use

   Tags

   Expression

   Bacterial

   Mutation

   Promoter

   Clostridium thermocellum DSM1313 Cellobiose Phosp…

   Available since

   March 19, 2020

   Availability

   Academic Institutions and Nonprofits only

   

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4. pENTR-Hu_TTK1(WT)

   Plasmid

   #136354

   Purpose

   Human TTK (Mps1) cDNA in Gateway pENTR

   Depositor

   Guillermo de Cárcer

   Insert

   TTK (TTK Human)
   

   Use

   Gateway entry vector

   Tags

   Expression

   Mutation

   Promoter

   Available since

   Feb. 4, 2020

   Availability

   Academic Institutions and Nonprofits only

   

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5. pLV-EF1a-V5-Ccdc167

   Plasmid

   #120242

   Purpose

   Expresses V5-tagged Ccdc167 in lentiviral vector

   Depositor

   Larry Gerace

   Insert

   Ccdc167 (Ccdc167 Mouse)
   

   Use

   Lentiviral

   Tags

   V5

   Expression

   Mutation

   Promoter

   EF1a

   Available since

   Jan. 10, 2019

   Availability

   Academic Institutions and Nonprofits only

   

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6. IRES-EGFP-Flag-hRabin8

   Plasmid

   #118073

   Purpose

   Expresses Flag-tagged human Rabin8 in mammalian cells

   Depositor

   Ken-Ichi Takemaru

   Insert

   human Rabin8 (RAB3IP Human)
   

   Use

   Lentiviral

   Tags

   Flag

   Expression

   Mammalian

   Mutation

   Promoter

   EF1 alpha

   Available since

   Dec. 7, 2018

   Availability

   Academic Institutions and Nonprofits only

   

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7. pCSD_SpCas9MT3-FKBP_SL

   Plasmid

   #107302

   Purpose

   Expresses R1335K mutant SpCas9 fused to FKBP in mammalian cells

   Depositor

   Scot Wolfe

   Insert

   SpCas9
   

   Use

   CRISPR

   Tags

   FKBP

   Expression

   Mammalian

   Mutation

   R1335K and fused to FKBP

   Promoter

   CMV IE94

   Available since

   Nov. 15, 2018

   Availability

   Academic Institutions and Nonprofits only

   

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8. pCSD_FRB-TALETS3-3xFLAG-2xNLS

   Plasmid

   #107306

   Purpose

   Expresses TALE-VEGFA-TS3 fused to FRB in mammalian cells

   Depositor

   Scot Wolfe

   Insert

   TALE_VEGFA-TS3
   

   Use

   CRISPR

   Tags

   3x Flag, 2xNLS and FRB

   Expression

   Mammalian

   Mutation

   Promoter

   CMV IE94

   Available since

   Nov. 15, 2018

   Availability

   Academic Institutions and Nonprofits only

   

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9. pHH0103 CEP164 WW domain

   Plasmid

   #104276

   Purpose

   Bacterial expression of WW domain from CEP164

   Depositor

   Marius Sudol, Sachdev Sidhu

   Insert

   CEP164 WW domain (CEP164 Human)
   

   Use

   Tags

   GST

   Expression

   Bacterial

   Mutation

   codon optimized for expression in bacteria

   Promoter

   tac

   Available since

   Aug. 28, 2018

   Availability

   Academic Institutions and Nonprofits only

   

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10. pHH0103 CEP164 WW domain

    Plasmid

    #104300

    Purpose

    Bacterial expression of WW domain from CEP164

    Depositor

    Marius Sudol, Sachdev Sidhu

    Insert

    CEP164 WW domain (CEP164 Human)
    

    Use

    Tags

    GST

    Expression

    Bacterial

    Mutation

    codon optimized for expression in bacteria

    Promoter

    tac

    Available since

    Aug. 28, 2018

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

11. pBSIIKS_HIS3_I

    Plasmid

    #109062

    Purpose

    yeast shuttle vector with deletable CEN6-ARSH4 cassette in pBIIKS backbone. LoxP site upstream of HIS3. For teaching purposes.

    Depositor

    Colin Logie

    Insert

    HIS3 (HIS3 Budding Yeast, Zygosaccharomyces rouxii)
    

    Use

    Cre/Lox

    Tags

    Expression

    Bacterial and Yeast

    Mutation

    Promoter

    HIS3

    Available since

    Aug. 1, 2018

    Availability

    Academic Institutions and Nonprofits only

    

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12. hC73M-HA

    Plasmid

    #111528

    Purpose

    Expresses human KCNB1 C73M in mammalian cells

    Depositor

    Federico Sesti

    Insert

    human C73M-Kv2.1 + HA (KCNB1 Human)
    

    Use

    Tags

    HA

    Expression

    Mammalian

    Mutation

    C73M

    Promoter

    CMV

    Available since

    June 19, 2018

    Availability

    Academic Institutions and Nonprofits only

    

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13. hC73L-HA

    Plasmid

    #111527

    Purpose

    Expresses human KCNB1 C73L in mammalian cells

    Depositor

    Federico Sesti

    Insert

    human C73L-Kv2.1-HA (KCNB1 Human)
    

    Use

    Tags

    HA

    Expression

    Mammalian

    Mutation

    C73L

    Promoter

    CMV

    Available since

    June 5, 2018

    Availability

    Academic Institutions and Nonprofits only

    

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14. pHH0103 HECW2 WW domain #1

    Plasmid

    #104244

    Purpose

    Bacterial expression of WW domain #1 from HECW2

    Depositor

    Marius Sudol, Sachdev Sidhu

    Insert

    HECW2 WW domain #1 (HECW2 Human)
    

    Use

    Tags

    GST

    Expression

    Bacterial

    Mutation

    codon optimized for expression in bacteria

    Promoter

    tac

    Available since

    Feb. 23, 2018

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

15. His8:MBP-tev-Trp-eK-Flv

    Plasmid

    #83390

    Purpose

    bacterial expression vector pVP16 containing a derivative of E. coli lacZ fused with E. coli flavin binding protein MioC to be used in N-end rule in vitro ubiquitination studies

    Depositor

    Nico Dissmeyer

    Insert

    flavin binding protein MioC
    

    Use

    Synthetic Biology

    Tags

    3xHA, 8xHis, and MBP

    Expression

    Bacterial

    Mutation

    Promoter

    Available since

    April 21, 2017

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

16. His8:MBP-tev-Asp-eK-Flv

    Plasmid

    #83389

    Purpose

    bacterial expression vector pVP16 containing a derivative of E. coli lacZ fused with E. coli flavin binding protein MioC to be used in N-end rule in vitro ubiquitination studies

    Depositor

    Nico Dissmeyer

    Insert

    flavin binding protein MioC
    

    Use

    Synthetic Biology

    Tags

    3xHA, 8xHis, and MBP

    Expression

    Bacterial

    Mutation

    Promoter

    Available since

    April 21, 2017

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

17. His8:MBP-tev-His-eK-Flv

    Plasmid

    #83388

    Purpose

    bacterial expression vector pVP16 containing a derivative of E. coli lacZ fused with E. coli flavin binding protein MioC to be used in N-end rule in vitro ubiquitination studies

    Depositor

    Nico Dissmeyer

    Insert

    flavin binding protein MioC
    

    Use

    Synthetic Biology

    Tags

    3xHA, 8xHis, and MBP

    Expression

    Bacterial

    Mutation

    Promoter

    Available since

    April 21, 2017

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

18. His8:MBP-tev-Lys-eK-Flv

    Plasmid

    #83387

    Purpose

    bacterial expression vector pVP16 containing a derivative of E. coli lacZ fused with E. coli flavin binding protein MioC to be used in N-end rule in vitro ubiquitination studies

    Depositor

    Nico Dissmeyer

    Insert

    flavin binding protein MioC
    

    Use

    Synthetic Biology

    Tags

    3xHA, 8xHis, and MBP

    Expression

    Bacterial

    Mutation

    Promoter

    Available since

    April 21, 2017

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

19. His8:MBP-tev-Leu-eK-Flv

    Plasmid

    #83386

    Purpose

    bacterial expression vector pVP16 containing a derivative of E. coli lacZ fused with E. coli flavin binding protein MioC to be used in N-end rule in vitro ubiquitination studies

    Depositor

    Nico Dissmeyer

    Insert

    flavin binding protein MioC
    

    Use

    Synthetic Biology

    Tags

    3xHA, 8xHis, and MBP

    Expression

    Bacterial

    Mutation

    Promoter

    Available since

    April 21, 2017

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

20. His8:MBP-tev-Arg-eK-Flv

    Plasmid

    #83385

    Purpose

    bacterial expression vector pVP16 containing a derivative of E. coli lacZ fused with E. coli flavin binding protein MioC to be used in N-end rule in vitro ubiquitination studies

    Depositor

    Nico Dissmeyer

    Insert

    flavin binding protein MioC
    

    Use

    Synthetic Biology

    Tags

    3xHA, 8xHis, and MBP

    Expression

    Bacterial

    Mutation

    Promoter

    Available since

    April 21, 2017

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

21. His8:MBP-tev-Phe-eK-Flv

    Plasmid

    #83384

    Purpose

    bacterial expression vector pVP16 containing a derivative of E. coli lacZ fused with E. coli flavin binding protein MioC to be used in N-end rule in vitro ubiquitination studies

    Depositor

    Nico Dissmeyer

    Insert

    flavin binding protein MioC
    

    Use

    Synthetic Biology

    Tags

    3xHA, 8xHis, and MBP

    Expression

    Bacterial

    Mutation

    Promoter

    Available since

    April 21, 2017

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

22. pCASCADE-gltA1-gltA2-GapAP1

    Plasmid

    #87150

    Purpose

    gltA1-gltA2-GapAP1 targeting guide RNA E. coli , Low Phosphate Induction

    Depositor

    Michael Lynch

    Insert

    gltA1-gltA2-gapAP1 guide array
    

    Use

    Tags

    Expression

    Bacterial

    Mutation

    Promoter

    E . coli ugpB gene promoter, low phosphate induct…

    Available since

    March 31, 2017

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

23. pJM102: pHps2 RFP on pCM66T

    Plasmid

    #74741

    Purpose

    pCM66T with promoter pHps2 driving RFP expression (maxRFP = BBa_E1010)

    Depositor

    Mary Lidstrom

    Insert

    RFP (BioBrick part number BBa_E1010)
    

    Use

    Synthetic Biology

    Tags

    Expression

    Bacterial

    Mutation

    Promoter

    pHps2 (extracted from Methylovorus glucosetrophus…

    Available since

    May 16, 2016

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

24. pJK103

    Plasmid

    #73265

    Purpose

    Produces Acetobacter aceti 1023 hypothetical acetic acid resistance protein B, C-terminal His6 tag (AaAarBH6)

    Depositor

    T. J. Kappock

    Insert

    hypothetical acetic acid resistance protein B
    

    Use

    Tags

    His6

    Expression

    Bacterial

    Mutation

    Promoter

    T7

    Available since

    April 22, 2016

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

25. pJK610

    Plasmid

    #72531

    Purpose

    Produces Acetobacter aceti 1023 formylglycinamidine ribonucleotide synthetase glutaminase subunit (AaPurQ); large subunit wutg C-terminal His6 tag (AaPurLH6)

    Depositor

    T. J. Kappock

    Inserts

    formylglycinamidine ribonucleotide synthetase, glutaminase subunit
    formylglycinamidine ribonucleotide synthetase, large subunit
    

    Use

    Tags

    6xHis

    Expression

    Bacterial

    Mutation

    Promoter

    T7 and n/a

    Available since

    March 7, 2016

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

26. pJK540

    Plasmid

    #72446

    Purpose

    Produces Acetobacter aceti 1023 small hypothetical protein with N-terminal His6 tag (H6AaOrfX); N5-carboxyaminoimidazole ribonucleotide mutase (AaPurE1); NCAIR synthetase (AaPurK)

    Depositor

    T. J. Kappock

    Inserts

    hypothetical protein
    N5-carboxyaminoimidazole ribonucleotide mutase
    N5-carboxyaminoimidazole ribonucleotide synthetase
    

    Use

    Tags

    His6

    Expression

    Bacterial

    Mutation

    Promoter

    T7 and n/a

    Available since

    March 4, 2016

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

27. pJK552

    Plasmid

    #72451

    Purpose

    Produces Acetobacter aceti 1023 N5-carboxyaminoimidazole ribonucleotide mutase with inserted Val2 (AaPurE1-V2ins); N5-carboxyaminoimidazole ribonucleotide synthetase, Val319>Phe mutant (AaPurK-V319F)

    Depositor

    T. J. Kappock

    Inserts

    N5-carboxyaminoimidazole ribonucleotide mutase
    N5-carboxyaminoimidazole ribonucleotide synthetase
    

    Use

    Tags

    Expression

    Bacterial

    Mutation

    Val319 to Phe and inserted Val2

    Promoter

    T7

    Available since

    March 3, 2016

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

28. pJK141

    Plasmid

    #73316

    Purpose

    Encodes Crepis alpina delta-12 fatty acid acetylenase (vFAD2) with Pas2p signal sequence, c-myc tag, and His6-tag

    Depositor

    T. J. Kappock

    Insert

    delta-12 fatty acid acetylenase
    

    Use

    Tags

    His6, Pas2p signal sequence, and c-myc

    Expression

    Yeast

    Mutation

    synthetic gene

    Promoter

    AOX1

    Available since

    Feb. 26, 2016

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

29. pJK586

    Plasmid

    #72459

    Purpose

    Produces Acetobacter aceti 1023 N5-carboxyaminoimidazole ribonucleotide mutase, Trp34>Phe/Ser57>Thr/Trp165>Phe mutant (AaPurE1fwf-S57T)

    Depositor

    T. J. Kappock

    Insert

    N5-carboxyaminoimidazole ribonucleotide mutase
    

    Use

    Tags

    Expression

    Bacterial

    Mutation

    Mutation changes tryptophan-34 to phenylalanine, …

    Promoter

    T7

    Available since

    Feb. 23, 2016

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

30. pJK565

    Plasmid

    #72454

    Purpose

    Produces Acetobacter aceti 1023 N5-carboxyaminoimidazole ribonucleotide mutase, Trp34>Phe/His59>Ala/Trp165>Phe mutant (AaPurE1fwf-H59A)

    Depositor

    T. J. Kappock

    Insert

    N5-carboxyaminoimidazole ribonucleotide mutase
    

    Use

    Tags

    Expression

    Bacterial

    Mutation

    Mutation changes tryptophan-34 to phenylalanine, …

    Promoter

    T7

    Available since

    Feb. 23, 2016

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

31. pJK578

    Plasmid

    #72457

    Purpose

    Produces Acetobacter aceti 1023 N5-carboxyaminoimidazole ribonucleotide mutase, Trp34>Phe/Ser57>Ala/Trp165>Phe mutant (AaPurE1fwf-S57A)

    Depositor

    T. J. Kappock

    Insert

    N5-carboxyaminoimidazole ribonucleotide mutase
    

    Use

    Tags

    Expression

    Bacterial

    Mutation

    Mutation changes tryptophan-34 to phenylalanine, …

    Promoter

    T7

    Available since

    Feb. 5, 2016

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

32. pJK585

    Plasmid

    #72458

    Purpose

    Produces Acetobacter aceti 1023 N5-carboxyaminoimidazole ribonucleotide mutase, Trp34>Phe/Ser57>Cys/Trp165>Phe mutant (AaPurE1fwf-S57C)

    Depositor

    T. J. Kappock

    Insert

    N5-carboxyaminoimidazole ribonucleotide mutase
    

    Use

    Tags

    Expression

    Bacterial

    Mutation

    Mutation changes tryptophan-34 to phenylalanine, …

    Promoter

    T7

    Available since

    Jan. 29, 2016

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

33. pJK543

    Plasmid

    #72516

    Purpose

    Produces Acetobacter aceti 1023 formylglycinamidine ribonucleotide synthetase small subunit (AaPurS); large subunit (AaPurL)

    Depositor

    T. J. Kappock

    Inserts

    formylglycinamidine ribonucleotide synthetase, small subunit
    formylglycinamidine ribonucleotide synthetase, large subunit
    

    Use

    Tags

    Expression

    Bacterial

    Mutation

    contains additional Ala2

    Promoter

    T7

    Available since

    Jan. 29, 2016

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

34. pJK576

    Plasmid

    #72527

    Purpose

    Encodes Acetobacter aceti 1023 formylglycinamidine ribonucleotide synthetase small subunit (AaPurS); hypothetical protein associated with purSyQL cluster (AaOrfY)

    Depositor

    T. J. Kappock

    Inserts

    formylglycinamidine ribonucleotide synthetase, small subunit
    hypothetical protein in purSQL operon
    

    Use

    Gene synthesis

    Tags

    Expression

    Mutation

    synthetic gene

    Promoter

    n/a

    Available since

    Jan. 29, 2016

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

35. pJK602

    Plasmid

    #71607

    Purpose

    Produces Acetobacter aceti 1023 N5-carboxyaminoimidazole ribonucleotide mutase, His59>Asn/Tyr154>Phe mutant (AaPurE1-H59N,Y154F)

    Depositor

    T. J. Kappock

    Insert

    N5-carboxyaminoimidazole ribonucleotide mutase
    

    Use

    Tags

    Expression

    Bacterial

    Mutation

    changes histidine-59 to asparagine, tyrosine-154 …

    Promoter

    T7

    Available since

    Jan. 29, 2016

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

36. pJK603

    Plasmid

    #71608

    Purpose

    Produces Acetobacter aceti 1023 N5-carboxyaminoimidazole ribonucleotide mutase, Trp34>Phe/Tyr154>Phe mutant (AaPurE1fww-Y154F)

    Depositor

    T. J. Kappock

    Insert

    N5-carboxyaminoimidazole ribonucleotide mutase
    

    Use

    Tags

    Expression

    Bacterial

    Mutation

    changes tryptophan-34 to phenylalanine, tyrosine-…

    Promoter

    T7

    Available since

    Jan. 29, 2016

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

37. pJK663

    Plasmid

    #71612

    Purpose

    Produces Acetobacter aceti 1023 N5-carboxyaminoimidazole ribonucleotide mutase, Tyr154>Phe/Trp165>Phe mutant (AaPurE1wwf-Y154F)

    Depositor

    T. J. Kappock

    Insert

    N5-carboxyaminoimidazole ribonucleotide mutase
    

    Use

    Tags

    Expression

    Bacterial

    Mutation

    changes tyrosine-154 to phenylalanine, tryptophan…

    Promoter

    T7

    Available since

    Jan. 29, 2016

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

38. pJK665

    Plasmid

    #71614

    Purpose

    Produces Acetobacter aceti 1023 N5-carboxyaminoimidazole ribonucleotide mutase, Trp34>Phe/His59>Asn/Trp97>Tyr mutant (AaPurE1fyw-H59N)

    Depositor

    T. J. Kappock

    Insert

    N5-carboxyaminoimidazole ribonucleotide mutase
    

    Use

    Tags

    Expression

    Bacterial

    Mutation

    changes tryptophan-34 to phenylalanine, histidine…

    Promoter

    T7

    Available since

    Jan. 29, 2016

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

39. pJK510

    Plasmid

    #71600

    Purpose

    Produces Acetobacter aceti 1023 N5-carboxyaminoimidazole ribonucleotide mutase, His59>Asn/Trp165>Phe mutant (AaPurE1wwf-H59N)

    Depositor

    T. J. Kappock

    Insert

    N5-carboxyaminoimidazole ribonucleotide mutase
    

    Use

    Tags

    Expression

    Bacterial

    Mutation

    changes histidine-59 to asparagine, tryptophan-16…

    Promoter

    T7

    Available since

    Jan. 29, 2016

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

40. pattB-aubergine-WT-gf

    Plasmid

    #69447

    Purpose

    genomic fragment of Drosophila aubergine was cloned from w1 background and inserted into pattB plasmid to expression Aubergine

    Depositor

    Phillip Zamore

    Insert

    aubergine (aub Fly)
    

    Use

    Tags

    NA

    Expression

    Insect

    Mutation

    many SNPs exist in the promoter region of aubergi…

    Promoter

    aubergine

    Available since

    Oct. 6, 2015

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge