We narrowed to 9,854 results for: ada

1. pEGFP-IREShygro2

   Plasmid

   #153966

   Purpose

   Expresses EGFP in mammalian cells

   Depositor

   Hiroyuki Konishi

   Article

   Hyodo et al Cell Rep. 2020 Jan 28;30(4):1195-1207.e7

   Insert

   EGFP
   

   Use

   Tags

   N/A

   Expression

   Mammalian

   Mutation

   No mutation

   Promoter

   CMV promoter

   Available since

   July 22, 2020

   Availability

   Academic Institutions and Nonprofits only

   

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2. pcDNA3 S6K2 KR

   Plasmid

   #17730

   Depositor

   John Blenis

   Article

   Lee-Fruman et al Oncogene. 1999 Sep 9. 18(36):5108-14.

   Insert

   S6K2 (RPS6KB2 Human)
   

   Use

   Tags

   HA

   Expression

   Mammalian

   Mutation

   K99R: kinase dead mutant

   Promoter

   Available since

   April 14, 2008

   Availability

   Academic Institutions and Nonprofits only

3. px458 EQR

   Plasmid

   #101731

   Purpose

   Expresses a sgRNA and a Cas9 EQR variant that recognizes "NGAG" PAM motifs

   Depositor

   Andrew Holland

   Article

   Lambrus et al Methods Cell Biol. 2018;144:107-135. doi

   Insert

   SpCas9 EQR
   

   Use

   Tags

   3xFLAG-NLS and NLS

   Expression

   Mammalian

   Mutation

   EQR (D1135E, R1335Q, and T1337R mutations in Cas9)

   Promoter

   Available since

   Oct. 17, 2017

   Availability

   Academic Institutions and Nonprofits only

   

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4. pRS415_pGal-nCas9(H840A)

   Plasmid

   #79616

   Purpose

   Expresses nCas9(H840A) in yeast cells

   Depositor

   Akihiko Kondo

   Article

   Nishida et al Science. 2016 Aug 4. pii: aaf8729.

   Insert

   SpCas9
   

   Use

   Tags

   Expression

   Yeast

   Mutation

   H840A for nickase Cas9

   Promoter

   pGal1

   Available since

   Sept. 2, 2016

   Availability

   Academic Institutions and Nonprofits only

   

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5. sABE v3.22 Lenti_2

   Plasmid

   #209556

   Purpose

   pU6-SpCas9_sgRNA-pCMV-bpSV40_NLS-TadA-8e (2-76)-2xFRB

   Depositor

   Xue Gao

   Article

   Zeng et al Nat Commun. 2023 Sep 11;14(1):5573. doi:

   Insert

   SpCas9 sgRNA, TadA-8e (2-76)-2xFRB
   

   Use

   Tags

   Expression

   Mammalian

   Mutation

   Promoter

   pU6, pCMV

   Available since

   Jan. 2, 2024

   Availability

   Academic Institutions and Nonprofits only

   

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6. pCfuNca-Plastid

   Plasmid

   #190818

   Purpose

   Plastid targeting GFP with ble resistant gene for zeocin

   Depositor

   Peter Kroth

   Article

   Sprecher et al Algal Research. 2023 May; Vol 72: 103136

   Inserts

   Diatom plastid targeted GFP
   Bleomycin resistance gene
   

   Use

   Tags

   Expression

   Bacterial

   Mutation

   Promoter

   Cylindrotheca fusiformis fcp and Nitzschia captiv…

   Available since

   Aug. 14, 2023

   Availability

   Academic Institutions and Nonprofits only

   

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7. pEBG-TRAF2 (GST)

   Plasmid

   #21586

   Depositor

   John Kyriakis

   Article

   Chadee et al Mol Cell Biol. 2002 Feb . 22(3):737-49.

   Insert

   TNF receptor-associated factor 2 (TRAF2 Human)
   

   Use

   Tags

   GST

   Expression

   Mammalian

   Mutation

   Promoter

   Available since

   Nov. 24, 2009

   Availability

   Academic Institutions and Nonprofits only

8. px458_2A_GFP_sgRNA_ZNF598

   Plasmid

   #127121

   Depositor

   Thomas Tuschl

   Article

   Meyer et al Mol Cell. 2020 Mar 19;77(6):1193-1205.e5

   Insert

   gRNA ZNF598 (ZNF598 Human)
   

   Use

   CRISPR

   Tags

   Expression

   Mutation

   Promoter

   Available since

   July 26, 2019

   Availability

   Academic Institutions and Nonprofits only

   

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9. pLJM1 Flag Raptor HRas25

   Plasmid

   #26637

   Depositor

   David Sabatini

   Article

   Sancak et al Cell. 2010 Apr 16. 141(2):290-303.

   Insert

   raptor-HRas25 (RPTOR Human)
   

   Use

   Lentiviral

   Tags

   Flag

   Expression

   Mammalian

   Mutation

   Promoter

   Available since

   Nov. 16, 2010

   Availability

   Academic Institutions and Nonprofits only

10. pJW1354

    Plasmid

    #69490

    Purpose

    Gly-Ser-4xGly linker-degron-TEV-3xFLAG with 40 bp nhr-25 homology arms

    Depositor

    Jordan Ward

    Article

    Zhang et al Development. 2015 Nov 9. pii: dev.129635

    Insert

    GSGGGG spacer-Degron-TEV protease site-3x FLAG epitope
    

    Use

    CRISPR; Template to generate cassettes for -media…

    Tags

    Expression

    Mutation

    Promoter

    Available since

    Sept. 30, 2015

    Availability

    Academic Institutions and Nonprofits only

    

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11. LRP6 deltaN-pCS2-VSVG

    Plasmid

    #27281

    Depositor

    Xi He

    Article

    Tamai et al Mol Cell. 2004 Jan 16. 13(1):149-56.

    Insert

    low density lipoprotein receptor-related protein 6 (LRP6 Human)
    

    Use

    Xenopus injection

    Tags

    VSVG

    Expression

    Mutation

    contains amino acid residues 1370–1613 (extracell…

    Promoter

    Available since

    Jan. 26, 2011

    Availability

    Academic Institutions and Nonprofits only

    

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12. pPB-cT3G-cERP2-MEIOC

    Plasmid

    #222562

    Purpose

    PiggyBac transposon plasmid for doxycycline inducible expression of MEIOC

    Depositor

    George Church

    Article

    Pierson Smela et al bioRxiv 2024.05.31.596483

    Insert

    MEIOC (C17orf104 Human)
    

    Use

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    Available since

    Aug. 15, 2024

    Availability

    Academic Institutions and Nonprofits only

    

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13. px459 VRER

    Plasmid

    #101716

    Purpose

    sgRNA/SpCas9 expression plasmid with Cas9 VRER mutations (NGCG PAM)

    Depositor

    Andrew Holland

    Article

    Lambrus et al Methods Cell Biol. 2018;144:107-135. doi

    Insert

    SpCas9 VRER
    

    Use

    Tags

    3xFLAG-NLS and NLS

    Expression

    Mammalian

    Mutation

    VRER (D1135V, G1218R, R1335E and T1337R)

    Promoter

    Available since

    Oct. 20, 2017

    Availability

    Academic Institutions and Nonprofits only

    

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14. pET22b-GFP-CPDSalI

    Plasmid

    #38257

    Depositor

    Aimee Shen, Matthew Bogyo

    Article

    Shen et al PLoS One. 2009 Dec 2;4(12):e8119.

    Insert

    eGFP
    

    Use

    Tags

    6xHis and Vibrio cholerae MARTX toxin cysteine pr…

    Expression

    Bacterial

    Mutation

    cloned from pEGFPN3 (Clonetech)

    Promoter

    T7

    Available since

    July 20, 2012

    Availability

    Academic Institutions and Nonprofits only

15. pBad-HaloGFP-Ca0.1(Na0.1, 143-144)

    Plasmid

    #198599

    Purpose

    Green fluorescent Ca2+ ion indicator for bacterial expression.

    Depositor

    Robert Campbell

    Article

    Zhu et al Nat Chem Biol. 2023 Jan;19(1):38-44. doi

    Insert

    HaloGFP-143-144
    

    Use

    Tags

    Expression

    Bacterial

    Mutation

    Promoter

    araBAD promoter

    Available since

    April 14, 2023

    Availability

    Academic Institutions and Nonprofits only

    

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16. Clathrin-LCa-ECFP

    Plasmid

    #21742

    Depositor

    Xiaowei Zhuang

    Article

    Chen et al Proc Natl Acad Sci U S A. 2008 Aug 19. 1

    Insert

    Clathrin, light polypeptide (LCa) (Clta Mouse)
    

    Use

    Tags

    ECFP

    Expression

    Mammalian

    Mutation

    Promoter

    Available since

    Aug. 5, 2009

    Availability

    Academic Institutions and Nonprofits only

17. pRK-flag-USP19 1-1290

    Plasmid

    #78579

    Purpose

    Expression flag-USP19 1-1290 in mammalian cells

    Depositor

    Yihong Ye

    Article

    Lee et al Nat Cell Biol. 2016 Jul;18(7):765-76. do

    Insert

    flag-USP19 1-1290 (USP19 Human)
    

    Use

    Tags

    flag

    Expression

    Mammalian

    Mutation

    mutate 1291 residue to stop condon

    Promoter

    CMV

    Available since

    June 22, 2016

    Availability

    Academic Institutions and Nonprofits only

18. pMK401 (Hygro-P2A-mAID-Nluc)

    Plasmid

    #121196

    Purpose

    N-terminal tagging

    Depositor

    Masato Kanemaki

    Article

    Yesbolatova et al Methods. 2019 Apr 23. pii: S1046-2023(18

    Insert

    Hygro-P2A-mAID-Nluc
    

    Use

    CRISPR

    Tags

    Expression

    Mutation

    Promoter

    Available since

    Feb. 13, 2019

    Availability

    Academic Institutions and Nonprofits only

    

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19. EK0501 SFFV-mCherry-SGc(MYC.s1)cSG-EGFP-(3xMS2) (FLP-IN)

    Plasmid

    #191185

    Purpose

    Expression of MYC sensor in mammalian cells

    Depositor

    Xiaojing Gao

    Article

    Kaseniit et al Nat Biotechnol. 2022 Oct 5. pii: 10.1038

    Insert

    mCherry:MYC.s1:EGFP-3xMS2
    

    Use

    Tags

    Expression

    Mammalian

    Mutation

    WT

    Promoter

    SFFV

    Available since

    Oct. 26, 2022

    Availability

    Academic Institutions and Nonprofits only

    

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20. USP19 sgRNA2

    Plasmid

    #78586

    Purpose

    delete USP19 gene in human cell

    Depositor

    Yihong Ye

    Article

    Lee et al Nat Cell Biol. 2016 Jul;18(7):765-76. do

    Insert

    USP19 sgRNA2 (USP19 Human)
    

    Use

    CRISPR

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    CBh

    Available since

    June 29, 2016

    Availability

    Academic Institutions and Nonprofits only