We narrowed to 6,950 results for: ust

1. pcDNA4TO T21R-T2A-mCherry

   Plasmid

   #225585

   Purpose

   Mammalian expression vector for Doxycycline inducible expression of TRIM21 RING with a self-cleaving T2A-mCherry fluorescent expression reporter

   Depositor

   William McEwan

   Article

   Benn et al Science. 2024 Aug 30;385(6712):1009-1016

   Insert

   T21R-T2A-mCherry
   

   Use

   Tags

   mCherry

   Expression

   Mammalian

   Mutation

   Promoter

   CMV

   Available since

   Feb. 11, 2025

   Availability

   Academic Institutions and Nonprofits only

   

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2. pcDNA3puro-EGFP-FXR1-C (379-621)

   Plasmid

   #225451

   Purpose

   Express mEGFP-fusion protein of FXR1 isoform a (FXR1 fragment (379-621))

   Depositor

   Christine Mayr

   Article

   Chen et al Cell. 5 August 2024. doi: 10.1016/j.cell

   Insert

   FXR1 isoform a (FXR1 Human)
   

   Use

   Tags

   mEGFP

   Expression

   Mammalian

   Mutation

   C terminal fragment (379-621)

   Promoter

   CMV

   Available since

   Nov. 18, 2024

   Availability

   Academic Institutions and Nonprofits only

   

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3. pLYS6-METTL17-CtoS-FLAG

   Plasmid

   #220876

   Purpose

   Expressing METTL17-FLAG_Cys to Ser mutants

   Depositor

   Vamsi Mootha

   Article

   Ast et al Mol Cell. 2024 Jan 18;84(2):359-374.e8.

   Insert

   METTL17 (METTL17 Human)
   

   Use

   Lentiviral

   Tags

   FLAG

   Expression

   Mammalian

   Mutation

   Changed Cys to Ser

   Promoter

   Available since

   Oct. 17, 2024

   Availability

   Academic Institutions and Nonprofits only

   

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4. pLYS6-METTL17-LYRMut-FLAG

   Plasmid

   #220877

   Purpose

   Expressing METTL17-LYRMut-FLAG

   Depositor

   Vamsi Mootha

   Article

   Ast et al Mol Cell. 2024 Jan 18;84(2):359-374.e8.

   Insert

   METTL17 (METTL17 Human)
   

   Use

   Lentiviral

   Tags

   FLAG

   Expression

   Mammalian

   Mutation

   Changed the LYP domain to AAA

   Promoter

   Available since

   Oct. 17, 2024

   Availability

   Academic Institutions and Nonprofits only

   

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5. psiCHECK-1-miR-455-WT

   Plasmid

   #215881

   Purpose

   This plasmid encodes luciferase which has microRNA binding sites in 3'UTR for detecting the microRNA silencing activity.

   Depositor

   Kumiko Ui-Tei

   Article

   Tian et al RNA Biol. 2020 Feb;17(2):264-280. doi: 1

   Insert

   three tandem repeats of seed-matched (SM) sequence of miR-455-WT (MIR455 Human)
   

   Use

   Tags

   Expression

   Mammalian

   Mutation

   Promoter

   Available since

   Oct. 16, 2024

   Availability

   Academic Institutions and Nonprofits only

   

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6. psiCHECK-1-miR-376a1(3p)-I-type

   Plasmid

   #215883

   Purpose

   This plasmid encodes luciferase which has microRNA binding sites in 3'UTR for detecting the microRNA silencing activity.

   Depositor

   Kumiko Ui-Tei

   Article

   Tian et al RNA Biol. 2020 Feb;17(2):264-280. doi: 1

   Insert

   three tandem repeats of seed-matched (SM) sequence of miR-376a1(3p)-I-type (MIR376A1 Human)
   

   Use

   Tags

   Expression

   Mammalian

   Mutation

   Promoter

   Available since

   Oct. 16, 2024

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

7. psiCHECK-1-miR-589(5p)-I-type

   Plasmid

   #215884

   Purpose

   This plasmid encodes luciferase which has microRNA binding sites in 3'UTR for detecting the microRNA silencing activity.

   Depositor

   Kumiko Ui-Tei

   Article

   Tian et al RNA Biol. 2020 Feb;17(2):264-280. doi: 1

   Insert

   three tandem repeats of seed-matched (SM) sequence of miR-589(5p)-I-type (MIR589 Human)
   

   Use

   Tags

   Expression

   Mammalian

   Mutation

   Promoter

   Available since

   Oct. 16, 2024

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

8. psiCHECK-1-miR-589(3p)-I-type

   Plasmid

   #215885

   Purpose

   This plasmid encodes luciferase which has microRNA binding sites in 3'UTR for detecting the microRNA silencing activity.

   Depositor

   Kumiko Ui-Tei

   Article

   Tian et al RNA Biol. 2020 Feb;17(2):264-280. doi: 1

   Insert

   three tandem repeats of seed-matched (SM) sequence of miR-589(3p)-I-type (MIR589 Human)
   

   Use

   Tags

   Expression

   Mammalian

   Mutation

   Promoter

   Available since

   Oct. 16, 2024

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

9. pcDNA3puro-mGFP-FXR1-N1-KH12mut (T236D H237D K299D N300D)

   Plasmid

   #225454

   Purpose

   Express mEGFP-fusion protein of FXR1 isoform a (T236D H237D K299D N300D)

   Depositor

   Christine Mayr

   Article

   Chen et al Cell. 5 August 2024. doi: 10.1016/j.cell

   Insert

   FXR1 isoform a (FXR1 Human)
   

   Use

   Tags

   mEGFP

   Expression

   Mammalian

   Mutation

   T236D H237D K299D N300D

   Promoter

   CMV

   Available since

   Oct. 10, 2024

   Availability

   Academic Institutions and Nonprofits only

   

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10. gRNA_SOX2_HDR

    Plasmid

    #163752

    Purpose

    gRNA expression vector to target the SOX2 stop codon for HDR gene tagging in human cells.

    Depositor

    Jennifer Mitchell

    Article

    Abatti et al Nucleic Acids Res. 2023 Sep 22:gkad734.

    Insert

    gRNA_SOX2 (SOX2 Human)
    

    Use

    CRISPR

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    U6

    Available since

    Oct. 3, 2024

    Availability

    Academic Institutions and Nonprofits only

    

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11. pBIOS1458

    Plasmid

    #171715

    Purpose

    expresses maize Glutamine Synthetase ZmGln1-3 cDNA under the control of the Cassava Vein Mosaic Virus promoter (CsVMV) (cassette 1) and the maize Rubisco small subunit promoter (ZmRbcS) (cassette 2)

    Depositor

    Christophe Sallaud

    Article

    Amiour et al Commun Biol. 2021 Sep 17;4(1):1095. doi:

    Insert

    ZmGln1-3
    

    Use

    Tags

    Expression

    Plant

    Mutation

    Promoter

    Available since

    Sept. 16, 2024

    Availability

    Academic Institutions and Nonprofits only

    

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12. lenti-KRAS-G121C/G13A-nick sgRNA

    Plasmid

    #214099

    Purpose

    Lentiviral vector expressing nicking sgRNA to induce KRAS G12C or G13A mutation using PE3

    Depositor

    Ting Zhou

    Article

    Wu et al Nat Commun. 2024 Dec 30;15(1):10824. doi

    Insert

    KRAS G12C or G13A nicking sgRNA
    

    Use

    Lentiviral

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    hU6

    Available since

    June 6, 2024

    Availability

    Academic Institutions and Nonprofits only

    

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13. AAVS1-iPE2-Hygro

    Plasmid

    #214019

    Purpose

    inducible PE2 system for controllable prime editing; this plasmid is used to insert PE2 prime editor in one allele of AAVS1 locus

    Depositor

    Ting Zhou

    Article

    Wu et al Nat Commun. 2024 Dec 30;15(1):10824. doi

    Insert

    PE2
    

    Use

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    TRE-tight

    Available since

    June 6, 2024

    Availability

    Academic Institutions and Nonprofits only

    

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14. mAMCase_CatD_6xHis_pcDNA3.1

    Plasmid

    #200229

    Purpose

    Expresses mouse Acidic Mammalian Chitinase catalytic domain with C-terminal 6xHis tag in pcDNA 3.1 vector

    Depositor

    James S Fraser

    Article

    Diaz et al Elife. 2024 Jun 17;12:RP89918. doi: 10.7

    Insert

    Acidic Mammalian Chitinase (Chia1 Mouse)
    

    Use

    Tags

    6xHis

    Expression

    Mammalian

    Mutation

    S354F

    Promoter

    CMV

    Available since

    Sept. 15, 2023

    Availability

    Academic Institutions and Nonprofits only

    

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15. p2T-CMV-miniSdd3-BE4max-BlastR

    Plasmid

    #204849

    Purpose

    For cytosine base editing using miniSdd3 in mammalian cells

    Depositor

    Caixia Gao

    Article

    Huang et al Cell. 2023 Jul 20;186(15):3182-3195.e14.

    Insert

    miniSdd3-nSpCas9(D10A)-UGI
    

    Use

    CRISPR

    Tags

    Expression

    Mammalian

    Mutation

    D10A in SpCas9

    Promoter

    CMV, SV40, EM7

    Available since

    Aug. 17, 2023

    Availability

    Academic Institutions and Nonprofits only

    

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16. pBVboostFGII WPRE 3C-CVB3

    Plasmid

    #202077

    Purpose

    Bacterial expression of 3C protease from coxsackievirus B3 (CVB3). Contains C-terminal His6-tag.

    Depositor

    Vesa Hytönen

    Article

    Jouppila et al Microbiol Spectr. 2023 Jun 15;11(3):e053

    Insert

    CVB3 3C protease with HisTag
    

    Use

    Tags

    6xHis-tag

    Expression

    Bacterial, Insect, and Mamm…

    Mutation

    Promoter

    polh, CAG, T7

    Available since

    Aug. 9, 2023

    Availability

    Academic Institutions and Nonprofits only

    

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17. pBVboostFGII WPRE 2A-CVB3

    Plasmid

    #202076

    Purpose

    Bacterial expression of 2A protease from coxsackievirus B3 (CVB3). Contains C-terminal His6-tag.

    Depositor

    Vesa Hytönen

    Article

    Jouppila et al Microbiol Spectr. 2023 Jun 15;11(3):e053

    Insert

    CVB3 2A protease with HisTag
    

    Use

    Tags

    6xHis-tag

    Expression

    Bacterial, Insect, and Mamm…

    Mutation

    Promoter

    polh, CAG, T7

    Available since

    Aug. 9, 2023

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

18. B*46:01:01

    Plasmid

    #203250

    Purpose

    Overexpress HLA allele

    Depositor

    Derin Keskin, Catherine Wu

    Article

    Sarkizova et al Nat Biotechnol. 2020 Feb;38(2):199-209.

    Insert

    B*46:01:01 (HLA-B Human)
    

    Use

    Lentiviral

    Tags

    Expression

    Mutation

    Promoter

    Available since

    July 10, 2023

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

19. B*53:01:01

    Plasmid

    #203249

    Purpose

    Overexpress HLA allele

    Depositor

    Derin Keskin, Catherine Wu

    Article

    Sarkizova et al Nat Biotechnol. 2020 Feb;38(2):199-209.

    Insert

    B*53:01:01 (HLA-B Human)
    

    Use

    Lentiviral

    Tags

    Expression

    Mutation

    Promoter

    Available since

    July 10, 2023

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

20. B*08:01:01

    Plasmid

    #203251

    Purpose

    Overexpress HLA allele

    Depositor

    Derin Keskin, Catherine Wu

    Article

    Sarkizova et al Nat Biotechnol. 2020 Feb;38(2):199-209.

    Insert

    B*08:01:01 (HLA-B Human)
    

    Use

    Lentiviral

    Tags

    Expression

    Mutation

    Promoter

    Available since

    July 10, 2023

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge