We narrowed to 18,356 results for: Tec

1. pSplit-ON-hDib1

   Plasmid

   #58787

   Purpose

   Express hDib1-LucN fusion protein in mammalian cells

   Depositor

   Hiroyoshi Ariga

   Article

   Maita et al Anal Biochem. 2014 Feb 3. pii: S0003-269

   Insert

   hDib1 (TXNL4A Human)
   

   Use

   Tags

   Flag and Luciferase (1-398aa)

   Expression

   Mammalian

   Mutation

   Promoter

   CMV

   Available since

   Aug. 15, 2014

   Availability

   Academic Institutions and Nonprofits only

2. Tet-O-FUW-Olig2

   Plasmid

   #45844

   Depositor

   Marius Wernig

   Article

   Yang et al Nat Biotechnol. 2013 May;31(5):434-9. do

   Insert

   Olig2 (Olig2 Mouse)
   

   Use

   Lentiviral

   Tags

   Expression

   Mammalian

   Mutation

   Promoter

   tet-ON

   Available since

   July 8, 2013

   Availability

   Academic Institutions and Nonprofits only

   

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3. pFastBac StrepII msfGFP TEV cloning vector with BioBrick PolyPromoter LIC Subcloning (438-Rgfp)

   Plasmid

   #55221

   Depositor

   Scott Gradia

   Article

   Gradia et al Methods Enzymol. 2017;592:1-26. doi: 10.

   Type

   Empty backbone

   Use

   Tags

   StrepII and msfGFP

   Expression

   Insect

   Mutation

   Promoter

   Available since

   June 17, 2014

   Availability

   Academic Institutions and Nonprofits only

   

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4. pS24·Pm-GFP

   Plasmid

   #122594

   Purpose

   Expresses GFP under the regulatory control of Pm. Expression needs additional xyls gene

   Depositor

   Pablo Ivan Nikel

   Article

   Volke et al Microb Biotechnol. 2019 Mar 12. doi: 10.

   Insert

   Pm promoter
   

   Use

   Synthetic Biology

   Tags

   Expression

   Bacterial

   Mutation

   Promoter

   Pm

   Available since

   March 22, 2019

   Availability

   Academic Institutions and Nonprofits only

   

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5. pJFRC7-syt1-GCaMP6f

   Plasmid

   #190896

   Purpose

   GCaMP6f fused to Drosophila synaptotagmin1 via a 3x GS linker. In the pJFRC7-20XUAS vector.

   Depositor

   Tom Clandinin

   Article

   Turner et al Elife. 2022 Oct 27;11. pii: 82587. doi:

   Insert

   syt1-GCaMP6f
   

   Use

   Tags

   Expression

   Insect

   Mutation

   Promoter

   Available since

   Nov. 18, 2022

   Availability

   Academic Institutions and Nonprofits only

   

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6. pCfB6574

   Plasmid

   #106132

   Purpose

   EasyCloneYALI system-based yeast integrative vector carrying loxP-flanked hygromycin-resistance marker, integration into Yarrowia lipolytica chromosomal location IntE_1, USER site for cloning, amp resistance

   Depositor

   Irina Borodina

   Article

   Holkenbrink et al Biotechnol J. 2018 Jan 29. doi: 10.1002/

   Type

   Empty backbone

   Use

   Tags

   Expression

   Yeast

   Mutation

   Promoter

   Available since

   April 6, 2018

   Availability

   Academic Institutions and Nonprofits only

   

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7. YIp128-P30-His-POL30

   Plasmid

   #99542

   Purpose

   Yeast integrative vector for expression of His-tagged POL30 (PCNA); LEU2 marker

   Depositor

   Helle Ulrich

   Article

   Davies et al Methods Mol Biol. 2012;920:543-67. doi:

   Insert

   POL30 (POL30 Budding Yeast)
   

   Use

   Tags

   6xHis

   Expression

   Yeast

   Mutation

   Promoter

   POL30

   Available since

   Sept. 14, 2017

   Availability

   Academic Institutions and Nonprofits only

   

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8. pFPL-Rg

   Plasmid

   #61654

   Purpose

   Gateway vector for generating red fluorescent protein fusions in fungi

   Depositor

   Mark Farman

   Article

   Gong et al Mol Plant Microbe Interact. 2014 Nov 12.

   Insert

   monomeric red fluorescent protein
   

   Use

   Tags

   Expression

   Plant

   Mutation

   Promoter

   Available since

   June 8, 2015

   Availability

   Academic Institutions and Nonprofits only

   

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9. mSA-phi29

   Plasmid

   #210815

   Purpose

   in vitro transcription of mRNA

   Depositor

   Erik Sontheimer

   Article

   Liu et al Nat Biotechnol. 2023 Sep 14. doi: 10.103

   Insert

   mSA-Phi29
   

   Use

   Tags

   Expression

   Bacterial

   Mutation

   Promoter

   Available since

   Feb. 28, 2024

   Availability

   Academic Institutions and Nonprofits only

   

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10. pTW211b5

    Plasmid

    #136927

    Purpose

    Directed evolution of SpCas9

    Depositor

    David Liu

    Article

    Miller et al Nat Biotechnol. 2020 Apr;38(4):471-481.

    Insert

    Cas9(N)-NpuN
    

    Use

    CRISPR

    Tags

    Expression

    Bacterial

    Mutation

    see manuscript

    Promoter

    Available since

    Feb. 11, 2020

    Availability

    Academic Institutions and Nonprofits only

    

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11. nCas9-NG_P2A-mCherry

    Plasmid

    #219934

    Purpose

    Mammalian expression of Sp-nCas9-NG with P2A-mCherry marker for use with MOBE systems

    Depositor

    Alexis Komor

    Article

    Cowan et al Nat Biotechnol. 2024 May 21. doi: 10.103

    Insert

    Sp-nCas9(D10A)-NG_P2A-mCherry
    

    Use

    CRISPR

    Tags

    SV40 bpNLS and SV40 bpNLS-P2A-mCherry

    Expression

    Mammalian

    Mutation

    nCas9(D10A)

    Promoter

    CMV

    Available since

    June 5, 2024

    Availability

    Academic Institutions and Nonprofits only

    

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12. pTK297

    Plasmid

    #59566

    Purpose

    SIN SP6 P1234 (nsP2 P726S) SGP(14) Kozak mKate-G8-L7Ae 4xFF4

    Depositor

    Ron Weiss

    Article

    Wroblewska et al Nat Biotechnol. 2015 Aug 3. doi: 10.1038

    Insert

    SGP(14) Kozak mKate-G8-L7Ae 4xFF4
    

    Use

    Tags

    Expression

    Mutation

    Promoter

    Available since

    Aug. 3, 2015

    Availability

    Academic Institutions and Nonprofits only

    

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13. pDM_T7_HisLacZomega

    Plasmid

    #118815

    Purpose

    T7 RNAP-driven expression of N-terminal His-tagged lacZ omega subunit.

    Depositor

    Alexander Green

    Article

    Ma et al Synth Biol (Oxf). 2018;3(1):ysy018. doi:

    Insert

    lacZ_
    

    Use

    Tags

    histag

    Expression

    Bacterial

    Mutation

    Promoter

    Available since

    Aug. 8, 2019

    Availability

    Academic Institutions and Nonprofits only

    

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14. pUC-DEST-hSOX2-EGFP-PuroTK

    Plasmid

    #141026

    Purpose

    Reporter integration

    Depositor

    Hideyuki Okano

    Article

    Yoshimatsu et al PLoS One. 2019 Aug 27;14(8):e0221164. do

    Insert

    EGFP
    

    Use

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    Available since

    July 9, 2020

    Availability

    Academic Institutions and Nonprofits only

    

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15. pCRISPomyces-Sth1Cas9

    Plasmid

    #129552

    Purpose

    Contains codon-optimized Sth1Cas9 and gRNA for streptomyces

    Depositor

    Mingzi M. Zhang, Fong Tian Wong

    Article

    Yeo et al Biotechnol Bioeng. 2019 May 15. doi: 10.

    Insert

    Sth1Cas9
    

    Use

    Tags

    Expression

    Bacterial

    Mutation

    Promoter

    rpsL(XC)-BbsI

    Available since

    Oct. 8, 2019

    Availability

    Academic Institutions and Nonprofits only

    

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16. PSuperPuroRetro-1660(hPot1)

    Plasmid

    #11135

    Depositor

    Christopher Counter

    Article

    Veldman et al Curr Biol. 2004 Dec 29. 14(24):2264-70.

    Insert

    RNAi against human Protection of Telomeres 1 (POT1 Human)
    

    Use

    RNAi and Retroviral

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    Available since

    March 3, 2006

    Availability

    Academic Institutions and Nonprofits only

17. pIM1708-surT-PsurP-agaN-pNW33N

    Plasmid

    #44009

    Depositor

    Ichiro Matsumura

    Article

    Blanchard et al Appl Microbiol Biotechnol. 2014 May 2.

    Insert

    surT-PsurP-agaN
    

    Use

    Tags

    Expression

    Bacterial

    Mutation

    Deleted EcoRI site (synonymous change, C to T at …

    Promoter

    Available since

    May 15, 2014

    Availability

    Academic Institutions and Nonprofits only

    

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18. pENTR-L1-PGK-TKBsd-L2

    Plasmid

    #141009

    Purpose

    Mutisite Gateway mediated vector construction

    Depositor

    Hideyuki Okano

    Article

    Yoshimatsu et al PLoS One. 2019 Aug 27;14(8):e0221164. do

    Insert

    TKBsd
    

    Use

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    Available since

    Aug. 4, 2020

    Availability

    Academic Institutions and Nonprofits only

    

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19. pColE1-ISceI

    Plasmid

    #179578

    Purpose

    Expresses I-SceI from an arabinose promoter

    Depositor

    Kevin Solomon

    Article

    Lee et al ACS Synth Biol. 2022 Jan 21;11(1):53-60.

    Insert

    I-SceI (I-SceI Budding Yeast)
    

    Use

    Tags

    Expression

    Bacterial

    Mutation

    Promoter

    Available since

    March 21, 2022

    Availability

    Academic Institutions and Nonprofits only

    

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20. MLM3639

    Plasmid

    #42252

    Purpose

    Cas9 expression vector

    Depositor

    Keith Joung

    Article

    Hwang et al Nat Biotechnol. 2013 Jan 29. doi: 10.103

    Insert

    Streptococcus pyogenes Cas9-3X Flag
    

    Use

    CRISPR; Zebrafish expression

    Tags

    3X Flag

    Expression

    Mutation

    Promoter

    CMV

    Available since

    March 4, 2013

    Availability

    Academic Institutions and Nonprofits only

    

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