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pmRFP_LAP2beta_IRES_puro2b

Plasmid

#21047

Depositor

Daniel Gerlich

Article

Steigemann et al Cell. 2009 Feb 6. 136(3):473-84.

Insert

LAP2_beta (TMPO Rat)

Tags

mRFP

Expression

Bacterial and Mammalian

Available Since

July 14, 2009

Availability

Academic Institutions and Nonprofits only

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pLSSmKate2-N1

Plasmid

#31867

Depositor

Vladislav Verkhusha

Article

Piatkevich et al Proc Natl Acad Sci U S A. 2010 Mar 23;10

Insert

LSS-mKate2

Expression

Mammalian

Mutation

K69Y/P131T/S148G/M167D/T183S/M196V

Promoter

CMV

Available Since

Sept. 13, 2011

Availability

Academic Institutions and Nonprofits only

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pCM157

Plasmid

#45863

Depositor

Mary Lidstrom

Article

Marx et al Biotechniques. 2002 Nov;33(5):1062-7.

Insert

cre recombinase

Use

Cre/Lox

Expression

Bacterial

Promoter

lac

Available Since

July 5, 2013

Availability

Academic Institutions and Nonprofits only

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pBAD-smURFP-RBS-HO-1

Plasmid

#80341

Purpose

Expresses smURFP and Heme Oxygenase-1 in bacterial cells.

Depositor

Erik Rodriguez, Roger Tsien

Article

Rodriguez et al Nat Methods. 2016 Aug 1. doi: 10.1038/nm

Inserts

smURFP
HO-1

Tags

6 Histidine Tag

Expression

Bacterial

Promoter

pBAD

Available Since

Aug. 5, 2016

Availability

Academic Institutions and Nonprofits only

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pHR-tdMCP-YFP

Plasmid

#99151

Purpose

Expresses dimeric MS2-coat binding protein tagged with YFP, 2nd generation lentiviral vector

Depositor

Ron Vale

Article

Jain et al Nature. 2017 Jun 8;546(7657):243-247. do

Insert

Dimeric MS2-coat binding protein

Use

Lentiviral

Tags

eYFP

Expression

Mammalian

Promoter

SFFV

Available Since

Aug. 8, 2017

Availability

Academic Institutions and Nonprofits only

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pGP-CMV-GCaMP6f

Plasmid

#40755

Purpose

Mammalian expression of ultrasensitive protein calcium sensor

Depositor

Douglas Kim, GENIE Project

Article

Chen et al Nature. 2013 Jul 18;499(7458):295-300. d

Insert

GCaMP6f

Tags

6xHis, T7 epitope, and Xpress tag

Expression

Mammalian

Promoter

CMV

Available Since

Oct. 29, 2012

Availability

Academic Institutions and Nonprofits only

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myrAkt delta4-129

Plasmid

#10841

Depositor

Richard Roth

Article

Kohn et al J Biol Chem. 1996 Sep 6. 271(36):21920-6

Insert

Akt (AKT1 Human)

Tags

HA and myr

Expression

Mammalian

Mutation

Deletion of PH domain (aa4-129)

Promoter

SV40

Available Since

Feb. 13, 2006

Availability

Academic Institutions and Nonprofits only

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1136- Desmoplakin-GFP

Plasmid

#32227

Depositor

Kathleen Green

Article

Godsel et al J Cell Biol. 2005 Dec 19;171(6):1045-59.

Insert

Desmoplakin (DSP Human)

Tags

EGFP

Expression

Mammalian

Promoter

CMV

Available Since

Nov. 8, 2011

Availability

Academic Institutions and Nonprofits only

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pCFD5

Plasmid

#73914

Purpose

multiplex gRNA plasmid

Depositor

Simon Bullock

Article

Port et al Nat Methods. 2016 Oct;13(10):852-4. doi:

Insert

dU6-3:tRNA:gRNA:tRNA:gRNA

Use

CRISPR

Expression

Insect

Promoter

dU6:3

Available Since

March 29, 2016

Availability

Academic Institutions and Nonprofits only

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GFP-TREX1(D18N)

Plasmid

#27220

Depositor

Judy Lieberman

Article

Yan et al Nat Immunol. 2010 Nov . 11(11):1005-13.

Insert

TREX1 (TREX1 Human)

Tags

GFP

Expression

Mammalian

Mutation

D18N sequence 52-GAC changed to AAC, resulting i…

Available Since

Jan. 26, 2011

Availability

Academic Institutions and Nonprofits only

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lentiCRISPR v2-sgControl

Plasmid

#125836

Purpose

KO control

Depositor

Boyi Gan

Article

Zhang et al Nat Cell Biol. 2018 Oct;20(10):1181-1192

Insert

Control

Use

Lentiviral

Available Since

June 5, 2019

Availability

Academic Institutions and Nonprofits only

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TFEB promoter-luciferase reporter

Plasmid

#66801

Purpose

Reporter for activity of TFEB gene promoter

Depositor

Albert La Spada

Article

Tsunemi et al Sci Transl Med. 2012 Jul 11;4(142):142ra

Insert

TFEB promoter (Tfeb Mouse)

Use

Luciferase

Available Since

June 22, 2015

Availability

Academic Institutions and Nonprofits only

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p414-TEF1p-Cas9-CYC1t

Plasmid

#43802

Purpose

A human codon-optimized Cas9 for expression in S. cerevisiae (budding yeast) from the TEF1 promoter

Depositor

George Church

Article

Dicarlo et al Nucleic Acids Res

Insert

Human Optimized S. pyogenes Cas9

Use

CRISPR

Expression

Yeast

Promoter

TEF1 promoter

Available Since

March 15, 2013

Availability

Academic Institutions and Nonprofits only

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pIRES2-EGFP-p53 WT

Plasmid

#49242

Purpose

expresses human wt p53 and EGFP in mammalian cells

Depositor

Dylan Taatjes

Article

Lin et al Aging Cell. 2013 Oct;12(5):863-72. doi:

Insert

p53 (TP53 Human)

Tags

IRES-EGFP

Expression

Mammalian

Promoter

CMV

Available Since

Dec. 27, 2013

Availability

Academic Institutions and Nonprofits only

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Nb-gp41-GFP (MoonTag-Nb-GFP)

Plasmid

#128602

Purpose

Expresses nanobody-2H10 fused to super folded GFP (sfGFP) in mammalian cells. It recognizes gp41 peptide array (MoonTag peptide array).

Depositor

Marvin Tanenbaum

Article

Boersma et al Cell. 2019 Jun 5. pii: S0092-8674(19)304

Insert

Nanobody_gp41

Use

Lentiviral

Tags

GB1 and sfGFP

Promoter

SFFV

Available Since

Oct. 30, 2019

Availability

Academic Institutions and Nonprofits only

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plenti-CAG -IRES-GFP

Plasmid

#69047

Purpose

Lentiviral backbone for GFP coexpression

Depositor

William Kaelin

Article

Lu et al Science. 2014 Jan 17;343(6168):305-9. do

Type

Empty backbone

Use

Lentiviral

Tags

IRES-GFP

Expression

Mammalian

Available Since

Oct. 17, 2017

Availability

Academic Institutions and Nonprofits only

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pAAV-EWB-DIO-myriRFP670V5-P2A-post-eGRASP

Plasmid

#111585

Purpose

An AAV vector that expresses double floxed myristoylated iRFP670 with V5 tag and post-eGRASP linked by self-cleaving P2A peptide under the Ef1a promoter.

Depositor

Bong-Kiun Kaang

Article

Choi et al Science. 2018 Apr 27;360(6387):430-435.

Insert

myriRFP670V5-P2A-post-eGRASP

Use

AAV

Promoter

Ef1a

Available Since

June 12, 2018

Availability

Academic Institutions and Nonprofits only

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pSG5-HA-p300

Plasmid

#89094

Purpose

mammalian expression of full-length human p300 in pSG5

Depositor

Elizabeth Wilson

Article

Askew et al J Biol Chem. 2010 Jul 9;285(28):21824-36

Insert

p300 (EP300 Human)

Tags

HA

Expression

Mammalian

Promoter

SV40

Available Since

April 21, 2017

Availability

Academic Institutions and Nonprofits only

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pLifeAct_mScarlet-i_N1

Plasmid

#85056

Purpose

In vivo visualization of actin cytoskeleton (can be used for colocalization studies)

Depositor

Dorus Gadella

Article

Bindels et al Nat Methods. 2016 Nov 21. doi: 10.1038/n

Insert

LifeAct

Expression

Mammalian

Promoter

CMV

Available Since

Dec. 6, 2016

Availability

Academic Institutions and Nonprofits only

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pCherry.90 alpha

Plasmid

#108222

Purpose

Mammalian expression vector for mCherry fusion to human Hsp90 alpha

Depositor

Didier Picard

Article

Lev et al Immunity. 2008 Jun;28(6):787-98. doi: 10

Insert

mCherry-Hsp90a (HSP90AA1 Human)

Expression

Mammalian

Promoter

CMV

Available Since

April 5, 2018

Availability

Academic Institutions and Nonprofits only

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