We narrowed to 11,284 results for: Vars;

1. pMXs-Sig10(M1)-Fc-IG

   Plasmid

   #183723

   Purpose

   Expresses the fusion protein containing the extracellular part of the human Siglec-10 R47Q mutant and the Fc region of human IgG1, and GFP

   Depositor

   Takeshi Tsubata

   Article

   Alborzian Deh Sheikh et al J Autoimmun. 2021 Jan;116:102571. doi: 1

   Insert

   Siglec-10
   

   Use

   Retroviral

   Tags

   Fc of human IgG1

   Mutation

   R47Q

   Available Since

   Oct. 14, 2022

   Availability

   Academic Institutions and Nonprofits only

   

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2. TTR C10S S85C G83R

   Plasmid

   #184747

   Purpose

   bacterial expression of human TTR mutants

   Depositor

   Peter Wright

   Article

   Ferguson et al Biochemistry. 2021 Mar 16;60(10):756-764

   Insert

   TTR (TTR Human)
   

   Expression

   Bacterial

   Mutation

   C10S, S85C, G83R

   Promoter

   T7

   Available Since

   Sept. 29, 2022

   Availability

   Academic Institutions and Nonprofits only

   

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3. pMXs-Sig10(M2)-Fc-IG

   Plasmid

   #183724

   Purpose

   Expresses the fusion protein containing the extracellular part of the human Siglec-10 A108V mutant and the Fc region of human IgG1, and GFP

   Depositor

   Takeshi Tsubata

   Article

   Alborzian Deh Sheikh et al J Autoimmun. 2021 Jan;116:102571. doi: 1

   Insert

   Siglec-10
   

   Use

   Retroviral

   Tags

   Fc of human IgG1

   Mutation

   A108V

   Available Since

   Sept. 28, 2022

   Availability

   Academic Institutions and Nonprofits only

   

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4. GFP-Rock1-GBD (840-110 aa) in pEGFPN1

   Plasmid

   #187279

   Purpose

   Express EGFP-Rock1 GTPase-binding domain

   Depositor

   Alpha Yap

   Article

   Budnar et al Dev Cell. 2019 Jun 17;49(6):894-906.e12.

   Insert

   Rock1 (Rock1 Mouse)
   

   Tags

   EGFP

   Expression

   Mammalian

   Mutation

   GBD (84-110aa)

   Promoter

   CMV

   Available Since

   Sept. 21, 2022

   Availability

   Academic Institutions and Nonprofits only

   

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5. TTR C10S S85C Y78F

   Plasmid

   #184746

   Purpose

   bacterial expression of human TTR mutants

   Depositor

   Peter Wright

   Article

   Ferguson et al Biochemistry. 2021 Mar 16;60(10):756-764

   Insert

   TTR (TTR Human)
   

   Expression

   Bacterial

   Mutation

   C10S, S85C, Y78F

   Promoter

   T7

   Available Since

   Aug. 23, 2022

   Availability

   Academic Institutions and Nonprofits only

   

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6. TTR C10S S85C A81T

   Plasmid

   #184745

   Purpose

   bacterial expression of human TTR mutants

   Depositor

   Peter Wright

   Article

   Ferguson et al Biochemistry. 2021 Mar 16;60(10):756-764

   Insert

   TTR (TTR Human)
   

   Expression

   Bacterial

   Mutation

   C10S, S85C, A81T

   Promoter

   T7

   Available Since

   Aug. 23, 2022

   Availability

   Academic Institutions and Nonprofits only

   

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7. TTR C10S S85C K80E

   Plasmid

   #184744

   Purpose

   bacterial expression of huamn TTR mutants

   Depositor

   Peter Wright

   Article

   Ferguson et al Biochemistry. 2021 Mar 16;60(10):756-764

   Insert

   TTR (TTR Human)
   

   Expression

   Bacterial

   Mutation

   C10S, S85C, K80E

   Promoter

   T7

   Available Since

   Aug. 23, 2022

   Availability

   Academic Institutions and Nonprofits only

   

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8. puc19_N_tag

   Plasmid

   #186650

   Purpose

   Empty donor plasmid.

   Depositor

   Astrid Haase

   Article

   Meng et al Nucleic Acids Res. 2022 May 27. pii: 659

   Insert

   N-terminal 3xFlag-3xHA Tag
   

   Tags

   3xFlag-3xHA

   Expression

   Insect

   Available Since

   July 22, 2022

   Availability

   Academic Institutions and Nonprofits only

   

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9. pST_A0A1D1UKG0_RAMVA (pBS0352)

   Plasmid

   #185188

   Purpose

   For the mammalian expression of the tardigrade protein A0A1D1UKG0_RAMVA. This is one of the bottom performing targets in our apoptosis assay (it induced apoptosis).

   Depositor

   Pamela Silver

   Article

   Veling et al ACS Synth Biol. 2022 Mar 18;11(3):1292-1

   Insert

   A0A1D1UKG0_RAMVA
   

   Expression

   Mammalian

   Available Since

   July 13, 2022

   Availability

   Academic Institutions and Nonprofits only

   

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10. pST_A0A1D1UJP0_RAMVA (pBS0306)

    Plasmid

    #185166

    Purpose

    For the mammalian expression of the tardigrade protein A0A1D1UJP0_RAMVA. This is one of the bottom performing targets in our apoptosis assay (it induced apoptosis).

    Depositor

    Pamela Silver

    Article

    Veling et al ACS Synth Biol. 2022 Mar 18;11(3):1292-1

    Insert

    A0A1D1UJP0_RAMVA
    

    Expression

    Mammalian

    Available Since

    July 13, 2022

    Availability

    Academic Institutions and Nonprofits only

    

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11. pST_CAHS2_RAMVA (pBS0291)

    Plasmid

    #185160

    Purpose

    For the mammalian expression of the tardigrade protein CAHS2_RAMVA. This is one of the top performing targets in our apoptosis assay (it protected from apoptosis).

    Depositor

    Pamela Silver

    Article

    Veling et al ACS Synth Biol. 2022 Mar 18;11(3):1292-1

    Insert

    CAHS2_RAMVA
    

    Expression

    Mammalian

    Available Since

    July 12, 2022

    Availability

    Academic Institutions and Nonprofits only

    

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12. pST_CAHS1_RAMVA (pBS0267)

    Plasmid

    #185150

    Purpose

    For the mammalian expression of the tardigrade protein CAHS1_RAMVA. This is one of the bottom performing targets in our apoptosis assay (it induced apoptosis).

    Depositor

    Pamela Silver

    Article

    Veling et al ACS Synth Biol. 2022 Mar 18;11(3):1292-1

    Insert

    CAHS1_RAMVA
    

    Expression

    Mammalian

    Available Since

    July 12, 2022

    Availability

    Academic Institutions and Nonprofits only

    

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13. A0A1D1UNQ6_RAMVA pSecTag2(pm) (pBS0256)

    Plasmid

    #185145

    Purpose

    For the mammalian expression of the tardigrade protein A0A1D1UNQ6_RAMVA. This is one of the bottom performing targets in our apoptosis assay (it induced apoptosis).

    Depositor

    Pamela Silver

    Article

    Veling et al ACS Synth Biol. 2022 Mar 18;11(3):1292-1

    Insert

    A0A1D1UNQ6_RAMVA
    

    Expression

    Mammalian

    Available Since

    July 12, 2022

    Availability

    Academic Institutions and Nonprofits only

    

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14. MAHS_RAMVA_Tanaka pSecTag (pBS0253)

    Plasmid

    #185142

    Purpose

    For the mammalian expression of the tardigrade protein MAHS_RAMVA. This is one of the bottom performing targets in our apoptosis assay (it induced apoptosis).

    Depositor

    Pamela Silver

    Article

    Veling et al ACS Synth Biol. 2022 Mar 18;11(3):1292-1

    Insert

    MAHS_RAMVA
    

    Expression

    Mammalian

    Available Since

    July 5, 2022

    Availability

    Academic Institutions and Nonprofits only

    

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15. SAHS10_A0A1D1UJR2_RAMVA pSecTag (pBS0250)

    Plasmid

    #185140

    Purpose

    For the mammalian expression of the tardigrade protein SAHS1_A0A1D1UJR2. This is one of the bottom performing targets in our apoptosis assay (it induced apoptosis).

    Depositor

    Pamela Silver

    Article

    Veling et al ACS Synth Biol. 2022 Mar 18;11(3):1292-1

    Insert

    SAHS1_A0A1D1UJR2
    

    Expression

    Mammalian

    Available Since

    July 5, 2022

    Availability

    Academic Institutions and Nonprofits only

    

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16. TTR C10S S85C I84S

    Plasmid

    #184748

    Purpose

    bacterial expression of human TTR mutants

    Depositor

    Peter Wright

    Article

    Ferguson et al Biochemistry. 2021 Mar 16;60(10):756-764

    Insert

    TTR (TTR Human)
    

    Expression

    Bacterial

    Mutation

    C10S, S85C, I84S

    Promoter

    T7

    Available Since

    July 5, 2022

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

17. pST_MAHS_RAMVA_mts_1-81 (pBS0918)

    Plasmid

    #185287

    Purpose

    For the mammalian expression of the tardigrade protein MAHS_RAMVA_mts_1-81. This is one of the bottom performing targets in our apoptosis assay (it induced apoptosis).

    Depositor

    Pamela Silver

    Article

    Veling et al ACS Synth Biol. 2022 Mar 18;11(3):1292-1

    Insert

    MAHS_RAMVA_mts_1-81
    

    Expression

    Mammalian

    Available Since

    June 9, 2022

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

18. pST_MAHS_RAMVA_mts_1-74 (pBS0917)

    Plasmid

    #185286

    Purpose

    For the mammalian expression of the tardigrade protein MAHS_RAMVA_mts_1-74. This is one of the bottom performing targets in our apoptosis assay (it induced apoptosis).

    Depositor

    Pamela Silver

    Article

    Veling et al ACS Synth Biol. 2022 Mar 18;11(3):1292-1

    Insert

    MAHS_RAMVA_mts_1-74
    

    Expression

    Mammalian

    Available Since

    June 9, 2022

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

19. pMXs-Sig10(DM)-Fc-IG

    Plasmid

    #183725

    Purpose

    Expresses the fusion protein containing the extracellular part of the human Siglec-10 R47Q A108V mutant and the Fc region of human IgG1, and GFP

    Depositor

    Takeshi Tsubata

    Article

    Alborzian Deh Sheikh et al J Autoimmun. 2021 Jan;116:102571. doi: 1

    Insert

    Siglec-10
    

    Use

    Retroviral

    Tags

    Fc of human IgG1

    Mutation

    R47Q and A108V

    Available Since

    June 7, 2022

    Availability

    Academic Institutions and Nonprofits only

    

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20. pJJGL003

    Plasmid

    #180607

    Purpose

    Plasmid expressing E. coli codon optimized His-MBP-TEV-DpbCasX+Region3 insertion

    Depositor

    Jennifer Doudna

    Article

    Tsuchida et al Mol Cell. 2022 Mar 17;82(6):1199-1209.e6

    Insert

    DpbCasX with R3 loop
    

    Use

    CRISPR

    Tags

    10x His and MBP

    Expression

    Bacterial

    Promoter

    T7

    Available Since

    April 25, 2022

    Availability

    Academic Institutions and Nonprofits only

    

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21. pK-eCFP

    Plasmid

    #176559

    Purpose

    Expression of eCFP for auxotrophic selection in the absence of histidine

    Depositor

    Markus Ralser

    Article

    Olin-Sandoval et al Nature. 2019 Aug;572(7768):249-253. doi:

    Insert

    eCFP
    

    Expression

    Yeast

    Promoter

    TDH1(GAP)

    Available Since

    Dec. 23, 2021

    Availability

    Academic Institutions and Nonprofits only

    

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22. LjUBQ:MtGLV1:T35s

    Plasmid

    #164738

    Purpose

    Overexpression of Peptide coding gene (Binary vector)

    Depositor

    Wolf Scheible

    Article

    Roy et al Plant J. 2024 May;118(3):607-625. doi: 1

    Insert

    Medtr6g043230.1
    

    Tags

    Terminator 35S

    Expression

    Plant

    Promoter

    Lotus japonicus Ubiquitin

    Available Since

    Dec. 17, 2021

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

23. LjUBQ:MtGLV9:T35s

    Plasmid

    #164742

    Purpose

    Overexpression of Peptide coding gene (Binary vector)

    Depositor

    Wolf Scheible

    Article

    Roy et al Plant J. 2024 May;118(3):607-625. doi: 1

    Insert

    Medtr4g116360.1
    

    Tags

    Terminator 35S

    Expression

    Plant

    Promoter

    Lotus japonicus Ubiquitin

    Available Since

    Dec. 16, 2021

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

24. LjUBQ:MtGLV10:T35s

    Plasmid

    #164739

    Purpose

    Overexpression of Peptide coding gene (Binary vector)

    Depositor

    Wolf Scheible

    Article

    Roy et al Plant J. 2024 May;118(3):607-625. doi: 1

    Insert

    Medtr8g446520.1
    

    Tags

    Terminator 35S

    Expression

    Plant

    Promoter

    Lotus japonicus Ubiquitin

    Available Since

    Dec. 13, 2021

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

25. CMV+ (mScarlet-I) plasmid with onboard mCitrine cassette

    Plasmid

    #169743

    Purpose

    CMV+ circuit plasmid that encodes mScarlet-I to report the circuit output levels. This plasmid also encodes a separate mCitrine expression cassette to monitor plasmid dosage.

    Depositor

    Francois St-Pierre

    Article

    Yang et al Nat Commun. 2021 Jul 5;12(1):4132. doi:

    Insert

    mScarlet-I
    

    Use

    Synthetic Biology

    Expression

    Mammalian

    Promoter

    pCMV-tetO2

    Available Since

    Oct. 14, 2021

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

26. LV_EF1a_RalA(G23V,full)-P2A-Hygro_Barcode

    Plasmid

    #170249

    Purpose

    Barcoded lentiviral vector to express RalA(G23V, full) in mammalian cells under control of EF1a promoter. Barcoded for transcript detection in single cell RNA-seq

    Depositor

    Prashant Mali

    Article

    Parekh et al iScience. 2021 Sep 20;24(10):103149. doi

    Insert

    RALA (RALA Human)
    

    Use

    Lentiviral

    Mutation

    G23V

    Promoter

    EF1a

    Available Since

    Sept. 23, 2021

    Availability

    Academic Institutions and Nonprofits only

    

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27. pGMC00008

    Plasmid

    #166724

    Purpose

    sgRNA cloning backbone

    Depositor

    Raj Chari

    Article

    Choi et al Nat Commun. 2020 Jun 1;11(1):2718. doi:

    Type

    Empty backbone

    Use

    CRISPR

    Expression

    Mammalian

    Available Since

    June 9, 2021

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

28. Equalizer-M plasmid with onboard mCherry cassette

    Plasmid

    #169734

    Purpose

    Equalizer-M plasmid that encodes eGFP to report circuit output levels. This plasmid also encodes a separate mCherry expression cassette to monitor plasmid dosage.

    Depositor

    Francois St-Pierre

    Article

    Yang et al Nat Commun. 2021 Jul 5;12(1):4132. doi:

    Insert

    tetR-P2A-eGFP-miR(FF4) target-miR(FF4)
    

    Use

    Synthetic Biology

    Expression

    Mammalian

    Promoter

    CMV-tetO2

    Available Since

    June 7, 2021

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

29. pMS5: pHelper(AvCAST)_entry_ΔTniQ

    Plasmid

    #168138

    Purpose

    Inducible expression of AvCAST proteins (except TniQ). Two BsaI sites for spacer cloning.

    Depositor

    Feng Zhang

    Article

    Saito et al Cell. 2021 Apr 29;184(9):2441-2453.e18.

    Inserts

    AvCAST minimal CRISPR array
    AvCAST Cascade proteins (Cas6, Cas8, Cas7 and Cas5)
    AvCAST Tns proteins (TnsA, TnsB and TnsC)
    AvTnsD
    

    Expression

    Bacterial

    Available Since

    June 2, 2021

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

30. pMS4: pHelper(AvCAST)_entry

    Plasmid

    #168137

    Purpose

    Inducible expression of AvCAST proteins. Two BsaI sites for spacer cloning.

    Depositor

    Feng Zhang

    Article

    Saito et al Cell. 2021 Apr 29;184(9):2441-2453.e18.

    Inserts

    AvCAST minimal CRISPR array
    AvCAST Cascade proteins (Cas6, Cas8, Cas7 and Cas5)
    AvCAST Tns proteins (TnsA, TnsB, TnsC and TniQ)
    AvTnsD
    

    Expression

    Bacterial

    Available Since

    June 2, 2021

    Availability

    Academic Institutions and Nonprofits only

    

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