Addgene

We narrowed to 30,392 results for: uros

Showing: 6241 - 6280 of 30392 results

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nAChR alpha4 YFP

Plasmid

#15245

Depositor

Henry Lester

Article

Nashmi et al J Neurosci. 2003 Dec 17. 23(37):11554-67

Insert

alpha4-YFP-M (Chrna4 Mouse)

Use

Tags

YFP

Expression

Mammalian

Mutation

Promoter

Available since

July 6, 2007

Availability

Academic Institutions and Nonprofits only
R711-X12-566: His6-MBP-tev-Hs.NF1iso2 (1203-1530)

Plasmid

#159580

Purpose

E. coli protein expression of His6-MBP-tev-Hs.NF1iso2 (1203-1530)

Depositor

Dominic Esposito

Article

Yan et al Cell Rep. 2020 Jul 21;32(3):107909. doi:

Insert

His6-MBP-tev-Hs.NF1iso2 (1203-1530) (NF1 Human)

Use

Tags

His6-MBP-tev

Expression

Bacterial

Mutation

Promoter

Available since

Sept. 28, 2020

Availability

Academic Institutions and Nonprofits only

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pOTTC590 - pCMV ER-iRFP

Plasmid

#59136

Purpose

A plasmid that express endoplasmic reticulum-targeted iRFP under the CMV promoter.

Depositor

Brandon Harvey

Article

Richie et al J Neurosci Methods. 2017 Jun 1;284:1-14.

Insert

ER-localized Infrared Fluorescent Protein

Use

Tags

ER retention signal and signal peptide

Expression

Mammalian

Mutation

Promoter

CMV-IE

Available since

Oct. 4, 2017

Availability

Academic Institutions and Nonprofits only

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pLV-CAG-F14F15S-Switch

Plasmid

#99733

Purpose

Flp-based FLEX reporter (from RFP to GFP)

Depositor

Ian Wickersham

Article

Chatterjee et al Nat Neurosci. 2018 Apr;21(4):638-646. do

Insert

TagRFP and EGFP

Use

Tags

Expression

Mammalian

Mutation

Promoter

CAG

Available since

Jan. 30, 2019

Availability

Academic Institutions and Nonprofits only

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pDisplay-GRAB_UCN1.0

Plasmid

#208667

Purpose

Expresses the genetically-encoded fluorescent urocortin (UCN) sensor GRAB_UCN1.0 in mammalian cells

Depositor

Yulong Li

Article

Wang et al Science. 2023 Nov 17;382(6672):eabq8173.

Insert

GPCR activation based urocortin (UCN) sensor GRAB_UCN1.0

Use

Tags

Expression

Mammalian

Mutation

Promoter

CMV

Available since

Jan. 16, 2024

Availability

Academic Institutions and Nonprofits only

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pJEP316-pAAV-U6SaCas9gRNA(SapI)-pA Empty Cassette

Plasmid

#113693

Purpose

U6 driven SaCas9 gRNA expression cassette without a gRNA. SapI can be used to clone in gRNAs.

Depositor

Jonathan Ploski

Article

Kumar et al Front Mol Neurosci. 2018 Nov 13;11:413.

Insert

SaCas9 gRNA Cassete

Use

AAV and CRISPR

Tags

Expression

Mutation

Promoter

Available since

Dec. 11, 2018

Availability

Academic Institutions and Nonprofits only

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CaV1.3e[8a,11,31b,Δ32,Δ42a]

Plasmid

#49332

Purpose

Repaired Addgene #26577 to agree with the rat ref sequence. Repairs are @: nt 6224 (GCC->GTC) [Ala->Val]; nt 3310 (GTG->GCG) [Val->Ala]; nt 731 (TCA->GGA) [Ser->Gly].

Depositor

Diane Lipscombe

Article

Xu et al J Neurosci. 2001 Aug 15. 21(16):5944-51.

Insert

CACNA1D (Cacna1d Rat)

Use

Tags

Expression

Mammalian

Mutation

Contains exons 8a, 11, and 31b.Exons 32 and 42a a…

Promoter

CMV

Available since

Feb. 3, 2014

Availability

Academic Institutions and Nonprofits only

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pBABE HA Chk2 KD, dominant negative chk2

Plasmid

#41902

Depositor

Stephen Elledge

Article

Matsuoka et al Science. 2007 May 25;316(5828):1160-6.

Insert

Chk2 (CHEK2 Human)

Use

Retroviral

Tags

HA

Expression

Mammalian

Mutation

D347A

Promoter

Available since

Nov. 27, 2018

Availability

Academic Institutions and Nonprofits only

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pLJM1-PHD2

Plasmid

#223552

Purpose

Lentivirus transfer plasmid for expression of full length human PHD2

Depositor

Somshuvra Mukhopadhyay

Article

Gurol et al Proc Natl Acad Sci U S A. 2024 Jun 25;12

Insert

PHD2 (EGLN1 Human)

Use

Lentiviral

Tags

HA

Expression

Mammalian

Mutation

Promoter

CMV

Available since

Sept. 23, 2024

Availability

Academic Institutions and Nonprofits only

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pCMV_NFL(K363TAG)-FLAG

Plasmid

#182656

Purpose

Expresses mouse neurofilament light chain with a TAG codon at the position K363 & C-terminal FLAG tag (DYKDDDDK) in mammalian cells. Can be used for amber codon suppression & click labeling of NFL.

Depositor

Ivana Nikić-Spiegel

Article

Arsic et al Nat Commun. 2022 Jan 14;13(1):314. doi:

Insert

mouse neurofilament light chain with a K363TAG mutation (Nefl Mouse)

Use

Tags

FLAG tag (DYKDDDDK)

Expression

Mammalian

Mutation

K363TAG

Promoter

CMV

Available since

May 31, 2022

Availability

Academic Institutions and Nonprofits only

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pAAV-hSyn-SOUL-P2A-TdTomato-WPRE-hGH-polyA

Plasmid

#177576

Purpose

Expresses SOUL in neuronal cell types

Depositor

Guoping Feng

Article

Gong et al Neuron. 2020 Jul 8;107(1):197. doi: 10.1

Insert

SOUL

Use

AAV

Tags

tdTomato

Expression

Mutation

Promoter

hSyn

Available since

Jan. 25, 2022

Availability

Academic Institutions and Nonprofits only

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pDisplay-GRAB_NTS1.0

Plasmid

#208670

Purpose

Expresses the genetically-encoded fluorescent neurotensin (NTS) sensor GRAB_NTS1.0 in mammalian cells

Depositor

Yulong Li

Article

Wang et al Science. 2023 Nov 17;382(6672):eabq8173.

Insert

GPCR activation based neurotensin (NTS) sensor GRAB_NTS1.0

Use

Tags

Expression

Mammalian

Mutation

Promoter

CMV

Available since

Oct. 24, 2023

Availability

Academic Institutions and Nonprofits only

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pAAV-syn-F14F15S-splitTVA-EGFP-tTAv2

Plasmid

#183352

Purpose

Flp-dependent helper virus for monosynaptic tracing; to be coinjected with pAAV-TREtight-mTagBFP2-B19G (Addgene #100799)

Depositor

Ian Wickersham

Article

Jin et al Nat Neurosci. 2024 Jan 11. doi: 10.1038/

Insert

TVA, EGFP and tTA

Use

AAV

Tags

Expression

Mutation

Promoter

Available since

Nov. 4, 2022

Availability

Academic Institutions and Nonprofits only

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AAV-actin prom-dsRed-adra2a.1227.shRNA

Plasmid

#67880

Purpose

expresses dsRed and shRNA to knock down adra2a receptors

Depositor

William Wisden

Article

Zhang et al Nat Neurosci. 2015 Apr;18(4):553-61. doi

Insert

dsRed and shRNA to knock down the mouse adra2 receptor

Use

AAV

Tags

Expression

Mammalian

Mutation

Promoter

chicken beta actin

Available since

Sept. 11, 2015

Availability

Academic Institutions and Nonprofits only
AAV-GFAP-NLS-CasRx-NLS-FLAG

Plasmid

#154000

Purpose

Express CasRx for AAV package

Depositor

Hui Yang

Article

Zhou et al Cell. 2020 Apr 30;181(3):590-603.e16. do

Insert

CasRx

Use

AAV and CRISPR

Tags

FLAG

Expression

Mutation

Promoter

GFAP

Available since

July 16, 2020

Availability

Academic Institutions and Nonprofits only

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pCIG-LRRC37Bchimpanzee-HA

Plasmid

#220794

Purpose

To express the chimpanzee receptor LRRC37B in mammalian cells together with EGFP

Depositor

Pierre Vanderhaeghen

Article

Libe-Philippot et al Cell. 2023 Dec 21;186(26):5766-5783.e25.

Insert

LRRC37B

Use

Tags

HA

Expression

Mutation

Promoter

Available since

Aug. 15, 2024

Availability

Academic Institutions and Nonprofits only

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pAAV-EF1a-FLEX(frt)-GCaMP6f-WPRE

Plasmid

#118273

Purpose

pAAV vector for flippase dependent GCaMP6f expression under the control of EF1a promoter

Depositor

Naoshige Uchida

Article

Menegas et al Nat Neurosci. 2018 Sep 3. pii: 10.1038/s

Insert

GCaMP6f

Use

AAV

Tags

6xHis (N terminal on insert), T7 epitope, and Xpr…

Expression

Mammalian

Mutation

Promoter

EF1a

Available since

Dec. 20, 2018

Availability

Academic Institutions and Nonprofits only

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pJEP302-pAAV-CMV-dSaCas9-KRAB-pA

Plasmid

#113677

Purpose

A CMV driven de-catalyzed SaCas9 fused to KRAB domain for inhibition of transcription in targeted region.

Depositor

Jonathan Ploski

Article

Kumar et al Front Mol Neurosci. 2018 Nov 13;11:413.

Insert

de-catalyzed SaCas9

Use

AAV and CRISPR

Tags

KRAB and NLS

Expression

Mutation

Promoter

Available since

Feb. 14, 2019

Availability

Academic Institutions and Nonprofits only

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pCRII-Topo Vglut2 in situ probe

Plasmid

#45639

Depositor

Jeffrey Macklis

Article

Azim et al Nat Neurosci. 2009 Oct;12(10):1238-47. d

Insert

Vglut2 in situ probe (Slc17a6 Mouse)

Use

In situ

Tags

Expression

Mutation

fragment contains nt 2477-2993 of slc17a6 (Vglut2…

Promoter

Available since

June 3, 2013

Availability

Academic Institutions and Nonprofits only
pJEP305-pAAV-EFS-dSaCas9-KRAB-pA

Plasmid

#113681

Purpose

A EFS driven de-catalyzed SaCas9 fused to KRAB domain for inhibition of transcription in targeted region.

Depositor

Jonathan Ploski

Article

Kumar et al Front Mol Neurosci. 2018 Nov 13;11:413.

Insert

de-catalyzed SaCas9

Use

AAV and CRISPR

Tags

KRAB and NLS

Expression

Mutation

Promoter

Available since

Jan. 22, 2019

Availability

Academic Institutions and Nonprofits only

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pCMS-eGFP-RTP801

Plasmid

#65057

Purpose

Expression of RTP801 in mammalian cells

Depositor

Lloyd Greene, Cristina Malagelada

Article

Malagelada et al J Neurosci. 2006 Sep 27;26(39):9996-1000

Insert

DDIT4 (Ddit4 Rat)

Use

Tags

EGFP

Expression

Mammalian

Mutation

Promoter

CMV-IE

Available since

May 19, 2015

Availability

Academic Institutions and Nonprofits only
pDisplay-GRAB_NPYmut

Plasmid

#208678

Purpose

Expresses the genetically-encoded fluorescent neuropeptide Y (NPY) control sensor GRAB_NPYmut in mammalian cells

Depositor

Yulong Li

Article

Wang et al Science. 2023 Nov 17;382(6672):eabq8173.

Insert

GPCR activation based neuropeptide Y (NPY) control sensor GRAB_NPYmut

Use

Tags

Expression

Mammalian

Mutation

Promoter

CMV

Available since

Oct. 24, 2023

Availability

Academic Institutions and Nonprofits only

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pCDH-EF1a-hLMX1A-T2A-copGFP

Plasmid

#170444

Purpose

Lentiviral vector expressing human LMX1A (with silent mutation) with copGFP reporter gene, under control of EF1alpha promoter.

Depositor

Sang-Hun Lee

Article

Wulansari et al Sci Adv. 2021 Feb 17;7(8). pii: 7/8/eabb

Insert

human LMX1A

Use

Lentiviral

Tags

Expression

Mutation

Promoter

EF1a

Available since

June 16, 2021

Availability

Academic Institutions and Nonprofits only

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pJEP303-pAAV-CMV-dSaCas9-VP64-pA

Plasmid

#113678

Purpose

A CMV driven de-catalyzed SaCas9 fused to VP64 domain for increased transcription in targeted region

Depositor

Jonathan Ploski

Article

Kumar et al Front Mol Neurosci. 2018 Nov 13;11:413.

Insert

de-catalyzed SaCas9

Use

AAV, CRISPR, and Synthetic Biology

Tags

NLS and VP64

Expression

Mutation

Promoter

Available since

Dec. 11, 2018

Availability

Academic Institutions and Nonprofits only

Enlarge
pOTTC592 - pCMV Mito-iRFP

Plasmid

#59135

Purpose

A plasmid that express mitochondrial-targeted iRFP under the CMV promoter.

Depositor

Brandon Harvey

Article

Richie et al J Neurosci Methods. 2017 Jun 1;284:1-14.

Insert

Mitochondria-localized Infrared Fluorescent Protein

Use

Tags

Mitochondria-targeting sequence

Expression

Mammalian

Mutation

Promoter

CMV-IE

Available since

Oct. 4, 2017

Availability

Academic Institutions and Nonprofits only

Enlarge
pOTTC591 - pCMV Mem-iRFP

Plasmid

#59134

Purpose

A plasmid that express membrane-targeted iRFP under the CMV promoter.

Depositor

Brandon Harvey

Article

Richie et al J Neurosci Methods. 2017 Jun 1;284:1-14.

Insert

Membrane-localized Infrared Fluorescent Protein

Use

Tags

Palmitylation signal

Expression

Mammalian

Mutation

Promoter

CMV-IE

Available since

Oct. 4, 2017

Availability

Academic Institutions and Nonprofits only

Enlarge
pAAV-hSyn-NOS-IN133.3xFLAG.mCherry-WPRE

Plasmid

#127868

Purpose

pAAV plasmid expressing an NOS-IN133.3xFLAG.mCherry fusion protein under the hSyn promoter

Depositor

Florian Freudenberg

Article

Candemir et al Eur Neuropsychopharmacol. 2016 Apr;26(4)

Insert

Nos1 (Nos1 Mouse)

Use

AAV

Tags

3xFLAG and mCherry

Expression

Mutation

Amino acids 1-133 only

Promoter

hSyn

Available since

Aug. 2, 2019

Availability

Academic Institutions and Nonprofits only

Enlarge
pcDNA3.1(+)_Granulin 2+linker3

Plasmid

#176920

Purpose

express Granulin 2 with linker 3 in mammalian cells

Depositor

Thomas Kukar

Article

Holler et al eNeuro. 2017 Aug 18;4(4). pii: eN-NWR-01

Insert

Granulin 2+linker3 (GRN Human)

Use

Tags

Twin-Strep and FLAG tags encoded after Signal Pep…

Expression

Mammalian

Mutation

Promoter

Available since

May 24, 2022

Availability

Academic Institutions and Nonprofits only

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pcDNA3.1(+)_Paragranulin-no linker

Plasmid

#176917

Purpose

express Paragranulin without linker 1 in mammalian cells

Depositor

Thomas Kukar

Article

Holler et al eNeuro. 2017 Aug 18;4(4). pii: eN-NWR-01

Insert

Paragranulin (GRN Human)

Use

Tags

Twin-Strep and FLAG tags encoded after Signal Pep…

Expression

Mammalian

Mutation

Promoter

Available since

Nov. 5, 2021

Availability

Academic Institutions and Nonprofits only

Enlarge
lentiCRISPR v2-sgCOQ2

Plasmid

#186025

Purpose

knock out COQ2 in mammalian cells

Depositor

Boyi Gan

Article

Mao et al Nature. 2021 May;593(7860):586-590. doi:

Insert

Coenzyme Q2, Polyprenyltransferase (COQ2 Human)

Use

CRISPR and Lentiviral

Tags

Expression

Mutation

Promoter

U6

Available since

June 21, 2022

Availability

Academic Institutions and Nonprofits only

Enlarge
pcDNA3.1(+)_Paragranulin+linker 1

Plasmid

#176915

Purpose

express Paragranulin with linker 1 in mammalian cells

Depositor

Thomas Kukar

Article

Holler et al eNeuro. 2017 Aug 18;4(4). pii: eN-NWR-01

Insert

Paragranulin + linker 1 (GRN Human)

Use

Tags

Twin-Strep and FLAG tags encoded after Signal Pep…

Expression

Mammalian

Mutation

Promoter

Available since

Nov. 5, 2021

Availability

Academic Institutions and Nonprofits only

Enlarge
pcDNA3.1(+)_Granulin 2-no linker

Plasmid

#176921

Purpose

express Granulin 2 without linker 3 in mammalian cells

Depositor

Thomas Kukar

Article

Holler et al eNeuro. 2017 Aug 18;4(4). pii: eN-NWR-01

Insert

Granulin 2 (GRN Human)

Use

Tags

Twin-Strep and FLAG tags encoded after Signal Pep…

Expression

Mammalian

Mutation

Promoter

Available since

May 24, 2022

Availability

Academic Institutions and Nonprofits only

Enlarge
pcDNA3.1(+)_Granulin 1+linker2

Plasmid

#176918

Purpose

express Granulin 1 with linker 2 in mammalian cells

Depositor

Thomas Kukar

Article

Holler et al eNeuro. 2017 Aug 18;4(4). pii: eN-NWR-01

Insert

Granulin 1+linker2 (GRN Human)

Use

Tags

Twin-Strep and FLAG tags encoded after Signal Pep…

Expression

Mammalian

Mutation

Promoter

Available since

May 24, 2022

Availability

Academic Institutions and Nonprofits only

Enlarge
nAChR alpha4 CFP

Plasmid

#15244

Depositor

Henry Lester

Article

Nashmi et al J Neurosci. 2003 Dec 17. 23(37):11554-67

Insert

alpha4-CFP-M (Chrna4 Mouse)

Use

Tags

CFP

Expression

Mammalian

Mutation

Promoter

Available since

July 6, 2007

Availability

Academic Institutions and Nonprofits only
pEGFP-N1-CATULIN

Plasmid

#118979

Purpose

Expresses full length of Catulin-GFP (Mus musculus). Contains CMV and T3 promoter for mammalian expression and mRNA production.

Depositor

Tomasz Prószyński

Article

Gingras et al J Cell Sci. 2016 Mar 1;129(5):898-911. d

Insert

Alpha Catulin (Ctnnal1 Mouse)

Use

Tags

GFP

Expression

Mammalian

Mutation

Promoter

Available since

Jan. 3, 2019

Availability

Academic Institutions and Nonprofits only

Enlarge
pcDNA3.1(+)_Granulin 4+linker5

Plasmid

#176924

Purpose

express Granulin 4 with linker 5 in mammalian cells

Depositor

Thomas Kukar

Article

Holler et al eNeuro. 2017 Aug 18;4(4). pii: eN-NWR-01

Insert

Granulin 4+linker5 (GRN Human)

Use

Tags

Twin-Strep and FLAG tags encoded after Signal Pep…

Expression

Mammalian

Mutation

Promoter

Available since

Nov. 5, 2021

Availability

Academic Institutions and Nonprofits only

Enlarge
pPRIME-CMV-GFP-shDyn2

Plasmid

#132712

Purpose

Encodes short hairpin RNA (shRNA) #2 that targets the 3’-untranslated region of the rat Pdyn gene

Depositor

Robert Messing

Article

Pomrenze et al Cell Rep. 2019 Oct 1;29(1):13-21.e4. doi

Insert

shDyn(2) (Pdyn Rat)

Use

Lentiviral

Tags

Expression

Mutation

Promoter

CMV

Available since

Oct. 25, 2019

Availability

Academic Institutions and Nonprofits only

Enlarge
MSCV-TAP(FLAG-HA) CYLD

Plasmid

#60031

Purpose

CYLD with N-terminal TAP-tag (HA,FLAG) and IRES-Puro to allow tagged protein to be maintained

Depositor

Stephen Elledge

Article

Stegmeier et al Proc Natl Acad Sci U S A. 2007 May 22. 1

Insert

Cylindromatosis (CYLD Human)

Use

Retroviral

Tags

FLAG, HA, and IRES Puro

Expression

Mammalian

Mutation

Promoter

LTR-driven expression

Available since

Feb. 24, 2016

Availability

Academic Institutions and Nonprofits only
b2AR->M71-IRES-tauGFP-ACNF TV

Plasmid

#105198

Purpose

targeting vector to generate a replacement of CDS of M71 with CDS of mouse beta2-adrenergic receptor with expression of tauGFP.

Depositor

Peter Mombaerts

Article

Omura et al Mol Cell Neurosci. 2014 Jan;58:1-10. doi

Insert

b2AR->M71-IRES-tauGFP-ACNF (Adrb2, Olfr151 Mouse)

Use

Mouse Targeting

Tags

Expression

Mutation

Promoter

Available since

May 29, 2018

Availability

Academic Institutions and Nonprofits only

Enlarge
pPRIME-CMV-GFP-shDyn1

Plasmid

#132711

Purpose

Encodes short hairpin RNA (shRNA) #1 that targets the 3’-untranslated region of the rat Pdyn gene

Depositor

Robert Messing

Article

Pomrenze et al Cell Rep. 2019 Oct 1;29(1):13-21.e4. doi

Insert

shDyn(1) (Pdyn Rat)

Use

Lentiviral

Tags

Expression

Mutation

Promoter

CMV

Available since

Oct. 25, 2019

Availability

Academic Institutions and Nonprofits only

Enlarge

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