We narrowed to 27,292 results for: Tat

1. pMXS-mitoGshF-blasticidin

   Plasmid

   #210314

   Purpose

   Expresses mitochondrially targeted, humanized Streptococcus thermophilus GshF in mammalian cells

   Depositor

   Kivanc Birsoy

   Article

   Wang et al Nature. 2021 Nov;599(7883):136-140. doi:

   Insert

   Bifunctional glutamate-cysteine ligase/glutathione synthetase GshF (gshAB Streptococcus thermophilus)
   

   Use

   Retroviral

   Tags

   FLAG peptide, Homo sapiens ACO2 mitochondrial tar…

   Expression

   Mutation

   Codon optimized for expression in human cells

   Promoter

   Available since

   Jan. 9, 2024

   Availability

   Academic Institutions and Nonprofits only

   

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2. pAAV-hSyn-DIO-FLAG-KORD-P2A-3xHA-hM3D(Gq)

   Plasmid

   #220611

   Purpose

   Neuron-specific, Cre-dependent co-expression of FLAG-tagged inhibitory KORD DREADD receptor, and 3x HA-tagged excitatory hM3D(Gq) DREADD receptor.

   Depositor

   Sam Golden

   Article

   Szelenyi et al Proc Natl Acad Sci U S A. 2024 Aug 6;121

   Insert

   FLAG-KORD-P2A-3x HA-hM3D(Gq)
   

   Use

   AAV and Cre/Lox

   Tags

   FLAG; ArgiNLS

   Expression

   Mammalian

   Mutation

   Promoter

   hSyn

   Available since

   Aug. 1, 2024

   Availability

   Academic Institutions and Nonprofits only

   

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3. pQCXIZ GFP PODXL 5N>Q

   Plasmid

   #202419

   Purpose

   Expression of GFP-tagged PODXL with mutated N-linked glycosylation sites

   Depositor

   David Bryant

   Article

   Roman-Fernandez et al Sci Adv. 2023 Feb 3;9(5):eabq1858. doi:

   Insert

   PODXL (PODXL Human)
   

   Use

   Lentiviral

   Tags

   GFP

   Expression

   Mutation

   N33/43/104/144/360Q

   Promoter

   Available since

   July 5, 2023

   Availability

   Academic Institutions and Nonprofits only

   

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4. pcDNA 3.1 KBTBD4 WT

   Plasmid

   #184627

   Purpose

   Mammalian expression vector with N-terminal 3xFlag for KBTBD4 WT expression in mammalian cells

   Depositor

   Vincenzo D'Angiolella

   Article

   Chen et al Cell Death Differ. 2022 Apr 4. pii: 10.1

   Insert

   N-terminal 3xFlag tagged KBTBD4 WT (KBTBD4 Human)
   

   Use

   Tags

   Expression

   Mammalian

   Mutation

   no mutation

   Promoter

   CMV

   Available since

   June 3, 2022

   Availability

   Academic Institutions and Nonprofits only

   

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5. pT2/shCol1a1/GFP4_Seq1.3

   Plasmid

   #206136

   Purpose

   Knockdown of Collagen Type 1 alpha 1. Construct has inverted repeats to be used in Sleeping Beauty transposon system.

   Depositor

   Maria Castro

   Article

   Comba et al Nat Commun. 2022 Jun 24;13(1):3606. doi:

   Insert

   COL1A1 (Col1a1 Mouse)
   

   Use

   Tags

   Expression

   Mammalian

   Mutation

   Promoter

   Available since

   Sept. 11, 2023

   Availability

   Academic Institutions and Nonprofits only

   

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6. pIS1 mLPIN1

   Plasmid

   #60803

   Purpose

   Luciferase reporter to measure miR-155 mediated differential repression. Contains LPIN1 3' UTR and mutated miR-155 sites

   Depositor

   David Bartel

   Article

   Nam et al Mol Cell. 2014 Mar 20;53(6):1031-43. doi

   Insert

   LPIN1 3'UTR and mutated miR-155 binding site (LPIN1 Human)
   

   Use

   Luciferase

   Tags

   Expression

   Mammalian

   Mutation

   mutated miR-155 binding site

   Promoter

   thymidine kinase (HSV-TK promoter)

   Available since

   Oct. 20, 2014

   Availability

   Academic Institutions and Nonprofits only

   

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7. pFastBac-M13-6His-KIF1C-GFP

   Plasmid

   #130975

   Purpose

   Expression of 6His-KIF1C-GFP in insect cells for protein purification.

   Depositor

   Anne Straube

   Article

   Siddiqui et al Nat Commun. 2019 Jun 19;10(1):2693. doi:

   Insert

   KIF1C-GFP (KIF1C Human)
   

   Use

   Tags

   6His and GFP

   Expression

   Insect

   Mutation

   5 silent mutations that make this construct RNAi …

   Promoter

   Polyhedrin

   Available since

   Oct. 15, 2019

   Availability

   Academic Institutions and Nonprofits only

   

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8. MAP3K3 gRNA (BRDN0001148097)

   Plasmid

   #76016

   Purpose

   3rd generation lentiviral gRNA plasmid targeting human MAP3K3

   Depositor

   David Root, John Doench

   Article

   Doench et al Nat Biotechnol. 2016 Jan 18. doi: 10.103

   Insert

   MAP3K3 (MAP3K3 Human)
   

   Use

   CRISPR and Lentiviral

   Tags

   Expression

   Mammalian

   Mutation

   Promoter

   hU6

   Available since

   July 6, 2016

   Availability

   Academic Institutions and Nonprofits only

   

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9. pcDNA.3.1.ADRB2-NP

   Plasmid

   #134376

   Purpose

   Nanoluc complementation assay. Expression of adrenoceptor beta 2 fused at C termimus with Natural peptide (NP) of NanoLuc. Addition of signal sequence and Flag epitope at N terminus of ADRB2.

   Depositor

   Julien Hanson

   Article

   Laschet et al J Biol Chem. 2019 Mar 15;294(11):4079-40

   Insert

   ADRB2-NP (ADRB2 Human)
   

   Use

   Tags

   Flag, natural peptide of nanoluciferase, and sign…

   Expression

   Bacterial and Mammalian

   Mutation

   Promoter

   T7

   Available since

   Aug. 3, 2020

   Availability

   Academic Institutions and Nonprofits only

   

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10. TFORF2656

    Plasmid

    #143597

    Purpose

    Lentiviral vector for overexpressing transcription factor ORFs with unique 24-bp barcodes. Barcodes facilitate identification of transcription factors in pooled screens.

    Depositor

    Feng Zhang

    Article

    Joung et al Cell. 2023 Jan 5;186(1):209-229.e26. doi

    Insert

    STAT3 (STAT3 Human)
    

    Use

    Lentiviral

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    EF1a

    Available since

    July 11, 2023

    Availability

    Academic Institutions and Nonprofits only

    

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11. AID-C12*-BE4max

    Plasmid

    #216729

    Purpose

    Expresses a BE4max base editing construct comprised of the hyperactive AID-C12* and nCas9 (Cas9 with D10A mutation) in mammalian cells.

    Depositor

    Rahul Kohli

    Article

    Berrios et al Nucleic Acids Res. 2024 Jan 23:gkae024.

    Insert

    AID-C12*-BE4max (AICDA Human)
    

    Use

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    CMV

    Available since

    April 12, 2024

    Availability

    Academic Institutions and Nonprofits only

    

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12. TFORF2657

    Plasmid

    #144061

    Purpose

    Lentiviral vector for overexpressing transcription factor ORFs with unique 24-bp barcodes. Barcodes facilitate identification of transcription factors in pooled screens.

    Depositor

    Feng Zhang

    Article

    Joung et al Cell. 2023 Jan 5;186(1):209-229.e26. doi

    Insert

    STAT3 (STAT3 Human)
    

    Use

    Lentiviral

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    EF1a

    Available since

    July 11, 2023

    Availability

    Academic Institutions and Nonprofits only

    

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13. plenti-ef1a-dCasRx-FTO-HA-T2A-BSD

    Plasmid

    #177120

    Purpose

    dCasRx mediated RNA tethering of FTO

    Depositor

    Wenbo Li

    Article

    Lee et al Mol Cell. 2021 Aug 19;81(16):3368-3385.e

    Insert

    dCasRx-FTO
    

    Use

    Lentiviral

    Tags

    HA and SV40 NLS

    Expression

    Mutation

    dCasRX contains R239A/H244A/R858A/H863A mutations…

    Promoter

    Ef1a

    Available since

    Nov. 24, 2021

    Availability

    Academic Institutions and Nonprofits only

    

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14. pCW57.1 KBTBD4 WT

    Plasmid

    #184624

    Purpose

    Doxycycline inducible lentiviral vector for N-terminal Flag tagged KBTBD4 WT expression in mammalian cells

    Depositor

    Vincenzo D'Angiolella

    Article

    Chen et al Cell Death Differ. 2022 Apr 4. pii: 10.1

    Insert

    N-terminal Flag tagged KBTBD4 WT (KBTBD4 Human)
    

    Use

    Lentiviral; Doxycycline inducible

    Tags

    Expression

    Mammalian

    Mutation

    no mutation

    Promoter

    TRE promoter, Tet ON

    Available since

    June 3, 2022

    Availability

    Academic Institutions and Nonprofits only

    

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15. pMXS-IRES-BLAST flag-CAD S1859A

    Plasmid

    #169821

    Purpose

    Expresses C-terminal flag-tagged CAD with mutation of reported S6K phosphorylation site

    Depositor

    Richard Possemato

    Article

    Shin et al Nat Metab. 2023 Feb 6. doi: 10.1038/s422

    Insert

    CAD (CAD Human)
    

    Use

    Retroviral

    Tags

    Flag tag

    Expression

    Mammalian

    Mutation

    S1859A; TCCC -> AGTC silent mutations at nt527…

    Promoter

    Available since

    July 15, 2024

    Availability

    Academic Institutions and Nonprofits only

    

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16. pcDNA3.1/FGFR2-S252W(Apert) myc

    Plasmid

    #33249

    Depositor

    Alka Mansukhani, Claudio Basilico

    Article

    Mansukhani et al J Cell Biol. 2000 Jun 12. 149(6):1297-30

    Insert

    FGFR2 (Fgfr2 Mouse)
    

    Use

    Tags

    His and Myc

    Expression

    Mammalian

    Mutation

    Mutation S252W in FGFR2 found in Apert syndrome. …

    Promoter

    CMV

    Available since

    Dec. 20, 2012

    Availability

    Academic Institutions and Nonprofits only

17. pAAV-hSyn-DIO-3xHA-hM3D(Gq)-P2A-ArgiNLS-oScarlet

    Plasmid

    #220608

    Purpose

    Neuron-specific, Cre-dependent co-expression of 3x HA-tagged excitatory hM3D(Gq) DREADD receptor, and a single-cell discriminating version of oScarlet as a reporter.

    Depositor

    Sam Golden

    Article

    Szelenyi et al Proc Natl Acad Sci U S A. 2024 Aug 6;121

    Insert

    3x HA-hM3D(Gq)-P2A-AgiNLS-oScarlet
    

    Use

    AAV and Cre/Lox

    Tags

    3x HA; ArgiNLS

    Expression

    Mammalian

    Mutation

    Promoter

    hSyn

    Available since

    Aug. 1, 2024

    Availability

    Academic Institutions and Nonprofits only

    

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18. pTriEx-PA-Rac1-C450A

    Plasmid

    #22025

    Purpose

    Expression of Rac1 fused with light-insensitive LOV2 (C450A mutation)

    Depositor

    Klaus Hahn

    Article

    Wu et al Nature. 2009 Sep 3. 461(7260):104-8.

    Insert

    PA-Rac1-C450A (RAC1 Human, Avena sativa (oat))
    

    Use

    Tags

    6X His

    Expression

    Bacterial, Insect, and Mamm…

    Mutation

    LOV domain contains light-insensitive mutation C4…

    Promoter

    CMV, p10

    Available since

    Sept. 29, 2009

    Availability

    Academic Institutions and Nonprofits only

19. pQCXIZ GFP PODXL 4K>R

    Plasmid

    #202420

    Purpose

    Expression of ubiquitination-deficient GFP-tagged PODXL (4 cytoplasmic lysines mutated to arginine)

    Depositor

    David Bryant

    Article

    Roman-Fernandez et al Sci Adv. 2023 Feb 3;9(5):eabq1858. doi:

    Insert

    PODXL (PODXL Human)
    

    Use

    Lentiviral

    Tags

    GFP

    Expression

    Mutation

    K431/525/526/547R

    Promoter

    Available since

    July 5, 2023

    Availability

    Academic Institutions and Nonprofits only

    

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20. pTriEx-mVenus-PA-Rac1-C450A

    Plasmid

    #22021

    Purpose

    Expression of Rac1 fused with light-insensitive LOV2 (C450A mutation)

    Depositor

    Klaus Hahn

    Article

    Wu et al Nature. 2009 Sep 3. 461(7260):104-8.

    Insert

    PA-Rac1-C450A (RAC1 Human, Avena sativa (oat))
    

    Use

    Tags

    6X His and mVenus

    Expression

    Bacterial, Insect, and Mamm…

    Mutation

    LOV domain contains the light-insensitive C450A m…

    Promoter

    CMV, p10

    Available since

    Sept. 29, 2009

    Availability

    Academic Institutions and Nonprofits only