We narrowed to 7,615 results for: Trac

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pGEM-HE-2xLyn-ERex-Venus-bPAC(F198Y)

Plasmid

#165489

Purpose

Xenopus oocyte expression of PACmn, a photoactivatable adenylyl cyclase derived from bPAC, membrane-anchored, no/reduced dark activity

Depositor

Thomas Oertner

Article

Yang et al BMC Biol. 2021 Oct 18;19(1):227. doi: 10

Insert

2xLyn-ERex-Venus-bPAC(F198Y)

Use

Cdna expression, xenopus oocyte

Tags

Venus

Expression

Bacterial

Promoter

T7

Available Since

Oct. 21, 2021

Availability

Academic Institutions and Nonprofits only

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pLentiGuide-Puro/CD73-gRNA4

Plasmid

#132588

Purpose

Lentiviral gRNA vector targeting human CD73

Depositor

Robert Sobol

Article

Wilk et al Sci Rep. 2020 Jan 20;10(1):651. doi: 10.

Insert

CD73 (NT5E Human)

Expression

Mammalian

Available Since

Sept. 24, 2021

Availability

Academic Institutions and Nonprofits only

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3611 pcDNA3 Bim

Plasmid

#8804

Depositor

Stanley Korsmeyer

Article

Harada et al Proc Natl Acad Sci U S A 2004 Oct 26;101

Insert

Bim (Bcl2l11 Mouse)

Expression

Mammalian

Available Since

March 23, 2006

Availability

Academic Institutions and Nonprofits only
EGFP-hPCM2w/b delta6

Plasmid

#47588

Depositor

Emanuel Strehler

Article

Chicka et al J Biol Chem. 2003 May 16;278(20):18464-7

Insert

PMCA2w/b delta6 (ATP2B2 Human)

Tags

EGFP-HA-His

Expression

Mammalian

Mutation

6 C-terminal AA deleted (needed for PDZ binding)

Promoter

CMV

Available Since

Oct. 4, 2013

Availability

Academic Institutions and Nonprofits only

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pSEM416 - mosTI unc-119 P_UPN - gfp(nls) - tbb-2

Plasmid

#182359

Purpose

Positive control for mosTI(unc-119). unc-119 rescue and pan-neuronal nuclear GFP expression after mosTI insertion.

Depositor

Christian Froekjaer-Jensen

Article

El Mouridi et al G3 (Bethesda). 2022 Jul 28. pii: 6651068

Insert

Ultra-pan-neuronal positive control pUNP::gfp(NLS)

Expression

Worm

Promoter

Promoter Ultra Pan Neuronal

Available Since

May 31, 2022

Availability

Academic Institutions and Nonprofits only

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pcDNA3.1-mGnptg

Plasmid

#78106

Purpose

Mammalian expression of mouse Gnptg with C-terminal His tag

Depositor

Thomas Braulke

Article

Encarnacao et al J Biol Chem. 2011 Feb 18;286(7):5311-8.

Insert

Gnptg (Gnptg Mouse)

Tags

His

Expression

Mammalian

Promoter

CMV

Available Since

May 19, 2016

Availability

Academic Institutions and Nonprofits only
pAAV gfaABC1D 2xLyn-ERex-Venus-bPAC(F198Y)

Plasmid

#171117

Purpose

Astrocyte expression of PACmn, a photoactivatable adenylyl cyclase derived from bPAC, membrane-anchored, no/reduced dark activity

Depositor

Thomas Oertner

Article

Yang et al BMC Biol. 2021 Oct 18;19(1):227. doi: 10

Insert

2xLyn-ERex-Venus-bPAC(F198Y)

Use

AAV

Tags

Venus

Expression

Mammalian

Promoter

gfaABC1D

Available Since

Oct. 22, 2021

Availability

Academic Institutions and Nonprofits only

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bArr1-ALFA

Plasmid

#201496

Purpose

Expression of the beta-arrestin1 containing a C-terminal ALFA tag.

Depositor

Patrick Giguère

Article

Zeghal et al J Biol Chem. 2023 Jul 28:105107. doi: 10

Insert

bArr1-ALFA (ARRB1 Human)

Tags

ALFA

Expression

Mammalian

Promoter

CMV

Available Since

Aug. 10, 2023

Availability

Academic Institutions and Nonprofits only

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GFP-IP3KC

Plasmid

#134603

Purpose

Expresses IP3KC GFP tagged

Depositor

Christophe Erneux

Article

Dewaste et al Biochem J. 2003 Aug 15;374(Pt 1):41-9.

Insert

IP3KC (ITPKC Human)

Tags

GFP

Expression

Mammalian

Available Since

Nov. 22, 2019

Availability

Academic Institutions and Nonprofits only

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pPB-CAG-3xFLAG::Fnip1-pgk-hph

Plasmid

#48758

Purpose

PiggyBac backbone, CAG promoter driving murine 3xFLAG-Fnip1 fusion, Hygromycin selection

Depositor

Austin Smith

Article

Betschinger et al Cell. 2013 Apr 11;153(2):335-47. doi: 10

Insert

3xFLAG::Fnip1 (Fnip1 Mouse)

Use

Piggybac

Tags

3xFLAG

Expression

Mammalian

Promoter

CAG

Available Since

Sept. 6, 2016

Availability

Academic Institutions and Nonprofits only
pGLTR-S-PURO

Plasmid

#58244

Purpose

GATEWAY-compatible lentiviral RNAi delivery vector; SFFV-driven Puromycin resistance

Depositor

Stephan Geley

Article

Sigl et al PLoS One. 2014 May 19;9(5):e97764. doi:

Inserts

attR1-Cm(R)-CcdB-attR2
Puro(R)

Use

Lentiviral and RNAi; Gateway destination vector

Expression

Mammalian

Promoter

SFFV and no

Available Since

Nov. 7, 2014

Availability

Academic Institutions and Nonprofits only

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pCin4-3xFLAG-MKL1 S454A

Plasmid

#19844

Depositor

Ron Prywes

Article

Muehlich et al Mol Cell Biol. 2008 Oct;28(20):6302-13.

Insert

MKL1 S454A (MRTFA Human)

Tags

3xFLAG

Expression

Mammalian

Mutation

changed Serine 454 to Alanine

Available Since

Dec. 16, 2008

Availability

Academic Institutions and Nonprofits only
pRSFDuet-1 IntS6 AA 1035-1284

Plasmid

#196904

Purpose

Expresses His-tagged Drosophila IntS6 amino acids 1035-1284

Depositor

Jeremy Wilusz

Article

Fujiwara et al Mol Cell. 2023 Nov 14:S1097-2765(23)0090

Insert

Integrator subunit 6 (IntS6 Fly)

Tags

6xHis

Expression

Bacterial

Available Since

Nov. 29, 2023

Availability

Academic Institutions and Nonprofits only

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pRevTRE MKL1 S449A/T450A/S454A

Plasmid

#19847

Depositor

Ron Prywes

Article

Muehlich et al Mol Cell Biol. 2008 Oct;28(20):6302-13.

Insert

MKL1 S449A/T450A/S454A (MRTFA Human)

Use

Retroviral

Tags

3xFLAG

Mutation

changed Serine 449, Threonine 450 and Serine 454 …

Available Since

Dec. 16, 2008

Availability

Academic Institutions and Nonprofits only
tetO-MESP1

Plasmid

#170694

Purpose

doxycycline-inducible overexpression of MESP1

Depositor

Paul Burridge

Article

Romero-Tejeda et al J Mol Cell Cardiol. 2023 Jul 6:S0022-282

Insert

MESP1 (MESP1 Human)

Use

Lentiviral; Doxycycline inducible

Expression

Mammalian

Promoter

TRE promoter, Tet-ON

Available Since

July 12, 2023

Availability

Academic Institutions and Nonprofits only

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pSpCas9_BB_2A-GFP_MAPRE1-gRNA#2

Plasmid

#107727

Purpose

Knock out of EB1 in human cells by CRISPR/Cas9

Depositor

Torsten Wittmann

Article

van Haren et al Nat Cell Biol. 2018 Jan 29. pii: 10.1038

Insert

MAPRE1 gRNA #1 (targets Exon 1) (MAPRE1 Human)

Expression

Mammalian

Promoter

U6

Available Since

April 30, 2018

Availability

Academic Institutions and Nonprofits only

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pRSFDuet-1 IntS11 AA 300-597

Plasmid

#199329

Purpose

Expresses His-tagged Drosophila IntS11 amino acids 300-597

Depositor

Jeremy Wilusz

Article

Fujiwara et al Mol Cell. 2023 Nov 14:S1097-2765(23)0090

Insert

Integrator subunit 11 (IntS11 Fly)

Tags

6xHis

Expression

Bacterial

Mutation

amino acids 300-597 only

Available Since

Nov. 29, 2023

Availability

Academic Institutions and Nonprofits only

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pB80-iLID-mCherry-RAB11

Plasmid

#174623

Purpose

Optogenetic coupling to recycling endosomes (RAB11) via iLID

Depositor

Lukas Kapitein

Article

Nijenhuis et al J Cell Biol. 2020 Apr 6;219(4). pii: 133

Insert

iLID-mCherry-RAB11 (RAB11A Human, Synthetic)

Tags

iLID-mCherry

Expression

Mammalian

Mutation

mCherry: Met1Del; RAB11: Met1Del

Promoter

Chicken beta-actin

Available Since

Oct. 14, 2021

Availability

Academic Institutions and Nonprofits only

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GFP-Braf R188L

Plasmid

#118330

Purpose

Expression of human Braf

Depositor

Philip Stork

Article

Li et al J Biol Chem. 2013 Sep 20;288(38):27646-5

Insert

Braf R188L (BRAF Human)

Tags

GFP

Expression

Mammalian

Mutation

R188L

Promoter

CMV

Available Since

Nov. 9, 2018

Availability

Academic Institutions and Nonprofits only

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pBS-Squ:mCherry

Plasmid

#20163

Depositor

Eric Wieschaus

Article

Martin et al Nature. 2009 Jan 22;457(7228):495-9. doi

Insert

spaghetti squash (squ, myosin II RLC) 5' UTR and ORF fused to mCherry followed by squ 3' UTR (sqh Fly)

Expression

Bacterial

Available Since

April 14, 2009

Availability

Academic Institutions and Nonprofits only