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pCDNA5/FRT_CMV_NES-Kinprola_JNK-mEGFP

Plasmid

#233359

Purpose

CMV driven expression of the JNK activity recorder Kinprola_JNK in mammalian cell lines fused to mEGFP

Depositor

Kai Johnsson

Article

Sun et al Nat Chem Biol. 2025 Jul 10. doi: 10.1038

Insert

NES-Kinprola_JNK-mEGFP

Tags

NES and mEGFP

Expression

Mammalian

Promoter

CMV

Available Since

March 26, 2025

Availability

Academic Institutions and Nonprofits only

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pCDNA5/FRT_CMV_NES-Kinprola_AMPK-mEGFP

Plasmid

#233361

Purpose

CMV driven expression of the AMPK activity recorder Kinprola_AMPK in mammalian cell lines fused to mEGFP

Depositor

Kai Johnsson

Article

Sun et al Nat Chem Biol. 2025 Jul 10. doi: 10.1038

Insert

NES-Kinprola_AMPK-mEGFP

Tags

NES and mEGFP

Expression

Mammalian

Promoter

CMV

Available Since

March 26, 2025

Availability

Academic Institutions and Nonprofits only

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pDECKO_Malat1_C

Plasmid

#72622

Purpose

Expresses two gRNAs targeting MALAT1 promoter

Depositor

Roderic Guigo, Rory Johnson

Article

Aparicio-Prat et al BMC Genomics. 2015 Oct 23;16(1):846. doi

Insert

gRNAs toward Malat1

Expression

Mammalian

Promoter

U6 (gRNA1) and H1 (gRNA2)

Available Since

June 14, 2016

Availability

Academic Institutions and Nonprofits only

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BII-Sh-FUCCI-Luc

Plasmid

#133364

Purpose

Piggybac vector for shRNA-mediated knockdown, co-expressed with FUCCI cell cycle reporter

Depositor

Blair Madison

Article

Fischer et al Am J Physiol Gastrointest Liver Physiol.

Insert

Clover-Geminin-IRES-mKO2-Cdt1

Tags

HA-tagged Clover-Geminin

Expression

Mammalian

Mutation

N/A

Promoter

CMV enhancer and hEf1a (CpG free)

Available Since

Jan. 3, 2020

Availability

Academic Institutions and Nonprofits only

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mCherry-Vimentin-C-18

Plasmid

#55157

Purpose

Localization: Intermediate Filaments, Excitation: 587, Emission: 610

Depositor

Michael Davidson

Article

Rizzo et al Cold Spring Harb Protoc. 2009 Dec;2009(1

Insert

Vimentin (VIM Human)

Tags

mCherry

Expression

Mammalian

Promoter

CMV

Available Since

Sept. 17, 2014

Availability

Academic Institutions and Nonprofits only
pyc111m

Plasmid

#158779

Purpose

ribosome-tagged, Cre-dependent GCaMP for soma localization

Depositor

Jennifer Garrison, Zachary Knight

Article

Chen et al Neuron. 2020 Jun 5. pii: S0896-6273(20)3

Insert

GCaMP6m-RPL10a

Use

AAV

Available Since

Sept. 28, 2020

Availability

Academic Institutions and Nonprofits only

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pSBbi_RP_MicroID2-FLAG-NES

Plasmid

#188525

Purpose

Encodes the biotin proximity labeling enzyme MicroID2 with a nuclear export sequence (NES) for expression and stable integration into mammalian cells

Depositor

James Londino

Article

Johnson et al Mol Cell Proteomics. 2022 Jun 7:100256.

Insert

MicroID2

Use

Transposon mediated gene integration

Tags

FLAG

Expression

Mammalian

Promoter

EF1a/RPBSA

Available Since

Sept. 22, 2022

Availability

Academic Institutions and Nonprofits only

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pET51b(+)-NADPH-LUCID-3 (C50 1.4 uM)

Plasmid

#117910

Purpose

Plasmid encoding the bacterial expression of the BRET semi-synthetic biosensor measuring the NADPH concentration with a C50 of 1.4 uM

Depositor

Kai Johnsson

Article

Yu et al Science. 2018 Sep 14;361(6407):1122-1126

Insert

SNAP_Pro30_[cpNLuc23_24]eDHFR(R44A)

Tags

His-tag and Strep-tag

Expression

Bacterial

Mutation

R44A on eDHFR

Promoter

T7

Available Since

Oct. 25, 2018

Availability

Academic Institutions and Nonprofits only

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mEmerald-PCNA-19

Plasmid

#54221

Purpose

Localization: Nucleus, Excitation: 487, Emission: 509

Depositor

Michael Davidson

Article

Lu et al J Cell Biol. 2010 Jun 14;189(6):967-80.

Insert

PCNA-19 (PCNA Human)

Tags

mEmerald

Expression

Mammalian

Promoter

CMV

Available Since

July 10, 2014

Availability

Industry, Academic Institutions, and Nonprofits
mTFP1-Vinculin-23

Plasmid

#55516

Purpose

Localization: Focal Adhesions, Excitation: 462, Emission: 492

Depositor

Michael Davidson

Article

Ai et al BMC Biol. 2008 Mar 6;6:13. doi: 10.1186/

Insert

Vinculin (VCL Human)

Tags

mTFP1

Expression

Mammalian

Promoter

CMV

Available Since

Oct. 2, 2014

Availability

Academic Institutions and Nonprofits only
pDECKO_UCA1

Plasmid

#72628

Purpose

Expresses two gRNAs targeting the UCA1 promoter

Depositor

Roderic Guigo, Rory Johnson

Article

Aparicio-Prat et al BMC Genomics. 2015 Oct 23;16(1):846. doi

Insert

gRNAs toward UCA1

Expression

Mammalian

Promoter

U6 (gRNA1) and H1 (gRNA2)

Available Since

June 14, 2016

Availability

Academic Institutions and Nonprofits only

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pIRES-Ap1g1-FKBP

Plasmid

#46945

Purpose

Co-expresses siRNA-resistant, FKBP-tagged AP-1 gamma subunit and G418 resistance gene in mammalian cells

Depositor

Margaret Robinson

Article

Robinson et al Dev Cell. 2010 Feb 16;18(2):324-31. doi:

Insert

Ap1g1-FKBP

Tags

Mainly mouse Ap1g1 cDNA, with a fragment of brain…

Expression

Mammalian

Available Since

Sept. 23, 2013

Availability

Academic Institutions and Nonprofits only

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MSCV-miRE_shBRD9_561-SV40-GFP

Plasmid

#75139

Purpose

Retroviral expression plasmid encoding shBRD9_561 (targeting human BRD)

Depositor

Christopher Vakoc

Article

Hohmann et al Nat Chem Biol. 2016 Jul 4. doi: 10.1038/

Insert

shBRD9_561

Use

Retroviral

Available Since

May 23, 2016

Availability

Academic Institutions and Nonprofits only
pENTRY-YFP

Plasmid

#15302

Depositor

William Hahn

Article

Boehm et al Cell. 2007 Jun 15. 129(6):1065-1079.

Insert

YFP

Use

Gateway entry

Available Since

July 12, 2007

Availability

Academic Institutions and Nonprofits only
mEos2-Integrin-Alpha2-N-18

Plasmid

#57389

Purpose

Localization: Integrin/Focal Adhesions, Excitation: 505 / 569, Emission: 516 / 581

Depositor

Michael Davidson

Article

McKinney et al Nat Methods. 2009 Feb . 6(2):131-3.

Insert

Integrin-Alpha2 (ITGA2 Human)

Tags

mEos2

Expression

Mammalian

Promoter

CMV

Available Since

Feb. 5, 2015

Availability

Industry, Academic Institutions, and Nonprofits
mEmerald-MyosinIIA-N-14

Plasmid

#54191

Purpose

Localization: Cytoskeleton, Excitation: 487, Emission: 509

Depositor

Michael Davidson

Article

Burnette et al J Cell Biol. 2014 Apr 14;205(1):83-96. d

Insert

MyosinIIA

Tags

mEmerald

Expression

Mammalian

Promoter

CMV

Available Since

July 25, 2014

Availability

Industry, Academic Institutions, and Nonprofits
2XMyc-LRRK2-K1347A

Plasmid

#25365

Depositor

Mark Cookson

Article

J Biol Chem. 2008 Jun 13;283(24):16906-1

Insert

LRRK2 (LRRK2 Human)

Tags

2XMyc

Expression

Mammalian

Mutation

Lysine 1347 mutated to Alanine

Available Since

June 22, 2010

Availability

Academic Institutions and Nonprofits only
eGFP-HA_BASU

Plasmid

#153996

Purpose

Express eGFP gene along with HA and BASU

Depositor

Paul Khavari

Article

Meyers et al PLoS Pathog. 2021 Oct 1;17(10):e1009412.

Insert

E-GFP

Use

Lentiviral

Expression

Mammalian

Available Since

Oct. 5, 2021

Availability

Academic Institutions and Nonprofits only

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mCerulean-Keratin-17

Plasmid

#55379

Purpose

Note- This plasmid contains Keratin 18. Localization: Cytokeratin, Excitation: 433, Emission: 475

Depositor

Michael Davidson

Article

Rizzo et al Cold Spring Harb Protoc. 2009 Dec;2009(1

Insert

Keratin (KRT18 Human)

Tags

mCerulean

Expression

Mammalian

Mutation

V282L

Promoter

CMV

Available Since

Oct. 7, 2014

Availability

Academic Institutions and Nonprofits only

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pQCXIP Flag-hRpt6

Plasmid

#19793

Depositor

Joan Conaway, Ronald Conaway

Article

Yao et al Mol Cell. 2008 Sep 26. 31(6):909-17.

Insert

hRpt6 (PSMC5 Human)

Use

Retroviral

Tags

Flag

Available Since

Nov. 21, 2008

Availability

Academic Institutions and Nonprofits only
pSIREN-RetroQ-shN

Plasmid

#73665

Purpose

pSIREN-RetroQ vector containing control sequence N

Depositor

Joe Landry

Article

Mayes et al Oncotarget. 2017 May 12;8(38):64344-6435

Insert

shRNA Neg Control

Use

Retroviral

Available Since

March 22, 2016

Availability

Industry, Academic Institutions, and Nonprofits

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mTagBFP2-Cx32-7

Plasmid

#55287

Purpose

Localization: Gap Junctions, Excitation: 399, Emission: 456

Depositor

Michael Davidson

Article

Subach et al PLoS One. 2011;6(12):e28674. Epub 2011 D

Insert

Cx32 (GJB1 Human)

Tags

mTagBFP2

Expression

Mammalian

Promoter

CMV

Available Since

Oct. 10, 2014

Availability

Industry, Academic Institutions, and Nonprofits

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pOG-Cre

Plasmid

#233265

Purpose

Plasmid expressing Cre recombinase that is used to insert DNA CARGO into RMCE homing locus in Rosa26

Depositor

Mauro Calabrese

Article

Trotman et al Mol Cell. 2025 Mar 20;85(6):1117-1133.e1

Insert

Cre recombinase

Use

Cre/Lox

Expression

Mammalian

Promoter

CMV

Available Since

March 25, 2025

Availability

Academic Institutions and Nonprofits only

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mEos2-CD9-10

Plasmid

#57356

Purpose

Localization: Tetraspanin/Membrane, Excitation: 505 / 569, Emission: 516 / 581

Depositor

Michael Davidson

Article

McKinney et al Nat Methods. 2009 Feb . 6(2):131-3.

Insert

CD9 (CD9 Human)

Tags

mEos2

Expression

Mammalian

Promoter

CMV

Available Since

Feb. 5, 2015

Availability

Industry, Academic Institutions, and Nonprofits
2XMyc-LRRK2-RCKW

Plasmid

#25064

Depositor

Mark Cookson

Article

J Biol Chem. 2008 Jun 13;283(24):16906-1

Insert

LRRK2 (LRRK2 Human)

Tags

2XMyc

Expression

Mammalian

Mutation

Contains only the ROC-COR-Kinase-WD40 domains …

Available Since

June 29, 2010

Availability

Academic Institutions and Nonprofits only

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Clover-PMP-N-10

Plasmid

#56305

Purpose

Localization: Peroxisomes, Excitation: 505, Emission: 515

Depositor

Michael Davidson

Article

Lam et al Nat Methods. 2012 Sep 9. doi: 10.1038/nm

Insert

PMP (PXMP2 Human)

Tags

mClover2

Expression

Mammalian

Promoter

CMV

Available Since

April 28, 2015

Availability

Industry, Academic Institutions, and Nonprofits

Enlarge
miniSOG-RatLC3

Plasmid

#57775

Purpose

Localization: Microtubule-Binding Protein, Excitation: 448 / 473, Emission: 500 / 528

Depositor

Michael Davidson

Article

Shu et al PLoS Biol. 2011 Apr;9(4):e1001041. doi:

Insert

LC3

Tags

miniSOG

Expression

Mammalian

Promoter

CMV

Available Since

Feb. 5, 2015

Availability

Industry, Academic Institutions, and Nonprofits
Clover-pBAD

Plasmid

#54575

Purpose

Localization: Protein Expression Vector , Excitation: 505, Emission: 515. This plasmid encodes Clover H231L based on reference from Bajar et. al. Sci Rep. 2016.

Depositor

Michael Davidson

Article

Lam et al Nat Methods. 2012 Sep 9. doi: 10.1038/nm

Type

Empty backbone

Tags

6xHis and Clover

Expression

Bacterial

Available Since

July 25, 2014

Availability

Industry, Academic Institutions, and Nonprofits
EGFP-ER-14

Plasmid

#56432

Purpose

Localization: Endoplasmic Reticulum, Excitation: 488, Emission: 507

Depositor

Michael Davidson

Article

Day et al Chem Soc Rev. 2009 Oct;38(10):2887-921.

Insert

ER (CALR Human)

Tags

EGFP

Expression

Mammalian

Mutation

ER Signal Peptide

Promoter

CMV

Available Since

Dec. 23, 2014

Availability

Industry, Academic Institutions, and Nonprofits
tdKatushka2-VASP-5

Plasmid

#56049

Purpose

Localization: Actin/Focal Adhesions, Excitation: 586, Emission: 632

Depositor

Michael Davidson

Article

Shcherbo et al Biochem J. 2009 Mar 15;418(3):567-74. do

Insert

VASP

Tags

tdKatushka2

Expression

Mammalian

Mutation

A209T in VASP

Promoter

CMV

Available Since

April 1, 2015

Availability

Industry, Academic Institutions, and Nonprofits

Enlarge
AsCas12a-DR-cr in PBCSK-

Plasmid

#126640

Purpose

Cloning vector AsCas12a DR-crRNA for expression in mammalian cells

Depositor

Scot Wolfe

Article

Liu et al Nucleic Acids Res. 2019 Mar 20. pii: 540

Insert

AsCas12a Full Length Direct Repeat crRNA

Use

CRISPR

Expression

Mammalian

Promoter

U6 Promoter

Available Since

June 27, 2019

Availability

Academic Institutions and Nonprofits only

Enlarge
2XMyc-LRRK2-WD40

Plasmid

#25073

Depositor

Mark Cookson

Article

J Biol Chem. 2008 Jun 13;283(24):16906-1

Insert

LRRK2 (LRRK2 Human)

Tags

2XMyc

Expression

Mammalian

Mutation

Contains only the WD40 domain aa 2155-2527

Available Since

Nov. 1, 2011

Availability

Academic Institutions and Nonprofits only
mRuby2-PMP-N-10

Plasmid

#55910

Purpose

Localization: Peroxisomes, Excitation: 559, Emission: 600

Depositor

Michael Davidson

Article

Lam et al Nat Methods. 2012 Sep 9. doi: 10.1038/nm

Insert

PMP (PXMP2 Human)

Tags

mRuby2

Expression

Mammalian

Promoter

CMV

Available Since

March 12, 2015

Availability

Industry, Academic Institutions, and Nonprofits

Enlarge
mEmerald-MyosinIIA-C-18

Plasmid

#54190

Purpose

Localization: Cytoskeleton, Excitation: 487, Emission: 509

Depositor

Michael Davidson

Article

Burnette et al J Cell Biol. 2014 Apr 14;205(1):83-96. d

Insert

MyosinIIA

Tags

mEmerald

Expression

Mammalian

Promoter

CMV

Available Since

July 10, 2014

Availability

Industry, Academic Institutions, and Nonprofits
mTagBFP2-Caveolin-C-10

Plasmid

#55279

Purpose

Localization: Membrane, Excitation: 399, Emission: 456

Depositor

Michael Davidson

Article

Subach et al PLoS One. 2011;6(12):e28674. Epub 2011 D

Insert

Caveolin (CAV1 Human)

Tags

mTagBFP2

Expression

Mammalian

Promoter

CMV

Available Since

Oct. 10, 2014

Availability

Industry, Academic Institutions, and Nonprofits
PA-mCherry-miniSOG-Lysosomes-20

Plasmid

#57793

Purpose

Localization: Lysosome Membrane, Excitation: 448 / 473, Emission: 500 / 528

Depositor

Michael Davidson

Article

Shu et al PLoS Biol. 2011 Apr;9(4):e1001041. doi:

Insert

LAMP1

Tags

PA-mCherry-miniSOG

Expression

Mammalian

Mutation

D50E in LAMP1

Promoter

CMV

Available Since

Jan. 29, 2015

Availability

Academic Institutions and Nonprofits only

Enlarge
PA-mCherry-miniSOG-Paxillin-22

Plasmid

#57796

Purpose

Localization: Focal Adhesions, Excitation: 448 / 473, Emission: 500 / 528

Depositor

Michael Davidson

Article

Shu et al PLoS Biol. 2011 Apr;9(4):e1001041. doi:

Insert

Paxillin

Tags

PA-mCherry-miniSOG

Expression

Mammalian

Promoter

CMV

Available Since

Feb. 5, 2015

Availability

Academic Institutions and Nonprofits only
pBacT7-S1T1L

Plasmid

#33292

Depositor

Terence Dermody

Article

Kobayashi et al Cell Host Microbe. 2007 Apr 19;1(2):147-

Insert

S1 (MoV1sS1gp2, MoV1sS1gp1 Orthoreovirus T1L)

Expression

Mammalian

Promoter

T7

Available Since

Dec. 5, 2011

Availability

Academic Institutions and Nonprofits only

Enlarge
mEmerald-H2B-C-10

Plasmid

#54112

Purpose

Localization: Nucleus/Histones, Excitation: 487, Emission: 509

Depositor

Michael Davidson

Article

Gao et al Cell. 2012 Dec 7;151(6):1370-85. doi: 10

Insert

H2B (H2BC11 Human)

Tags

mEmerald

Expression

Mammalian

Mutation

D26G and V119I in H2B

Promoter

CMV

Available Since

June 16, 2014

Availability

Industry, Academic Institutions, and Nonprofits
2XMyc-LRRK2-RQ/TG

Plasmid

#25369

Depositor

Mark Cookson

Article

J Biol Chem. 2008 Jun 13;283(24):16906-1

Insert

LRRK2 (LRRK2 Human)

Tags

2XMyc

Expression

Mammalian

Mutation

Threonine 1343 mutated to Glycine Arginine 139…

Available Since

June 29, 2010

Availability

Academic Institutions and Nonprofits only

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We narrowed to 52,592 results for: son