Skip to main content
Addgene
Open Menu

Enter a sequence to perform a BLAST-based search of Addgene plasmid sequence databases.

Information

In Category

Filter By

More Filters

We narrowed to 14,223 results for: Cas9

Clear keywords
Showing: 3141 - 3160 of 14223 results

  4. PEA1

    Plasmid
    #176527
    Purpose
    Delivers all prime editing nickase components in a single plasmid
    Depositor
    Paul Thomas
    Publication
    Adikusuma et al Nucleic Acids Res. 2021 Sep 17. pii: 637
    Has service
    50 µg Transfection-Ready DNA and 100 µg Transfection-Ready DNA
    Insert
    CbH-Cas9(H840A)-RT, hU6-pegRNA (Bbs1 gate), hU6-sgRNA (Bbs1 gate)
    Use
    CRISPR
    Expression
    Mammalian
    Promoter
    CbH for Cas9, hU6 for gRNAs
    Available since
    Dec. 16, 2021
    Availability
    Academic Institutions and Nonprofits only
    176527-plasmid-map-sequence-id-350558

    Enlarge

  5. HeFSpCas9

    Plasmid
    #92355
    Purpose
    Expression plasmid for human codon-optimized increased fidelity HeFSpCas9 (without U6-sgRNA coding sequence)
    Depositor
    Ervin Welker
    Publication
    Kulcsar et al Genome Biol. 2017 Oct 6;18(1):190. doi:
    Has service
    50 µg Transfection-Ready DNA and 100 µg Transfection-Ready DNA
    Insert
    3xFLAG-NLS-Streptococcus pyogenes Highly enhanced Fidelity Cas9-NLS
    Use
    CRISPR
    Tags
    3xFLAG and NLS
    Expression
    Mammalian
    Mutation
    N497A, R661A, Q695A, K848A, Q926A, K1003A, R1060A
    Promoter
    Cbh
    Available since
    Oct. 31, 2017
    Availability
    Academic Institutions and Nonprofits only
    92355-plasmid-map-sequence-id-197617

    Enlarge

  9. pJEP10-AAV-U6/TO-gRNA(Empty)-CMV-TetR-P2A-eGFP-KASH-pA

    Plasmid
    #82706
    Purpose
    AAV backbone with a full length U6/TO promoter driving expression of a sgRNA. The gRNA can be inserted via Sapl sites. TracrRNA compatible with SpCas9. gRNA transcription is doxycycline dependent.
    Depositor
    Jonathan Ploski
    Publication
    de Solis et al Front Mol Neurosci. 2016 Aug 18;9:70. do
    Insert
    U6/TO Empty gRNA Expression Cassette
    Use
    AAV and CRISPR
    Tags
    CMV promoter driving expression of TetR-P2A-GFP-K…
    Expression
    Mammalian
    Available since
    Dec. 20, 2016
    Availability
    Academic Institutions and Nonprofits only
    82706-plasmid-map-sequence-id-158046

    Enlarge

  11. pEN-dCas9-CDA1-UGI

    Plasmid
    #196248
    Purpose
    Plasmid containing promoter of RecA from Xanthomonas oryzae driving expression of dead Cas9, cytosine deaminase CDA1, and glycosylase inhibitor
    Depositor
    Bing Yang
    Publication
    Li et al Commun Biol. 2023 Jan 17;6(1):56. doi: 1
    Insert
    attL4-RecApro-dCas9-CDA1-UGI-attR1
    Expression
    Bacterial
    Promoter
    RecA promoter
    Available since
    Feb. 27, 2023
    Availability
    Academic Institutions and Nonprofits only
    196248-plasmid-map-sequence-id-385887

    Enlarge

  12. pUC19-Q1QGC9

    Plasmid
    #103121
    Purpose
    Cas9 coding gene template with optimized sequence for human codon usage
    Depositor
    Duhee Bang
    Publication
    Cho et al Nucleic Acids Res. 2018 May 18;46(9):e55
    Insert
    • Q1QGC9(Cas9 coding gene from Streptococcus thermophilus (strain ATCC BAA-491 / LMD-9))
    Use
    CRISPR; Cloning vector
    Mutation
    human codon-optimized
    Available since
    April 20, 2018
    Availability
    Academic Institutions and Nonprofits only
    103121-plasmid-map-sequence-id-201883

    Enlarge

  13. pUC19-Q6KIQ7

    Plasmid
    #103128
    Purpose
    Cas9 coding gene template with optimized sequence for human codon usage
    Depositor
    Duhee Bang
    Publication
    Cho et al Nucleic Acids Res. 2018 May 18;46(9):e55
    Insert
    Q6KIQ7(Cas9 coding gene from Mycoplasma mobile (strain ATCC 43663 / 163K / NCTC 11711))
    Use
    CRISPR; Cloning vector
    Mutation
    human codon-optimized
    Available since
    April 20, 2018
    Availability
    Academic Institutions and Nonprofits only
    103128-plasmid-map-sequence-id-201892

    Enlarge

  14. pHEE401

    Plasmid
    #71286
    Purpose
    Egg cell-specific promoter-controlled expression of 3×FLAG-NLS-zCas9-NLS. Contains gRNA scaffold for insertion of target sequence (U6-26 promoter), Hyg resistance
    Depositor
    Qi-Jun Chen
    Publication
    Wang et al Genome Biol. 2015 Jul 21;16:144. doi: 10
    Inserts
    sgRNA scaffold
    zCas9
    Use
    CRISPR; Plant binary vector
    Tags
    3x FLAG and NLS
    Expression
    Plant
    Mutation
    Zea mays codon-optimized Cas9
    Promoter
    EC1.2 promoter and U6-26p Arabidopsis U6 gene pro…
    Available since
    Jan. 15, 2016
    Availability
    Academic Institutions and Nonprofits only
    Cited by
    71286-plasmid-map-sequence-id-127777

    Enlarge

  15. pAAV-GFAP-spCas9

    Plasmid
    #231403
    Purpose
    Expresses spCas9 from GFAP promoter
    Depositor
    Arjen Boender, Larry Young
    Insert
    GFAP promoter (Gfap Mouse)
    Use
    AAV and CRISPR
    Expression
    Mammalian
    Promoter
    GFAP
    Available since
    Oct. 24, 2025
    Availability
    Academic Institutions and Nonprofits only
    231403-plasmid-map-sequence-id-457979

    Enlarge

  19. PX458_3XHA_dFnCas9_KRAB_T2A_eGFP

    Plasmid
    #200389
    Purpose
    3XHA tagged dead Cas9 from F. novicida with KRAB repressor domain, T2A self-cleaving peptide and enhanced GFP biomarker for mammalian expression
    Depositor
    Debojyoti Chakraborty
    Insert
    PX458_3XHA_dFnCas9_KRAB_eGFP
    Use
    CRISPR
    Tags
    3xHA-NLS and SV40NLS-KRAB-T2A-EGFP
    Expression
    Mammalian
    Promoter
    CBH
    Available since
    Sept. 11, 2023
    Availability
    Academic Institutions and Nonprofits only
    200389-plasmid-map-sequence-id-407168

    Enlarge

  20. pHL-H1-ccdB-mEF1a-RiH

    Plasmid
    #60601
    Purpose
    Cloning vector for CRISPR-sgRNA (into the BamHI-EcoRI site), expresses RFP and hygromycin resistance gene.
    Depositor
    Akitsu Hotta
    Publication
    Li et al Stem Cell Reports. 2014 Nov 25. pii: S22
    Has service
    50 µg Transfection-Ready DNA and 100 µg Transfection-Ready DNA
    Inserts
    Red Fluorescent Protein
    Hygromycin resistance gene
    Use
    CRISPR
    Expression
    Mammalian
    Available since
    Dec. 11, 2014
    Availability
    Academic Institutions and Nonprofits only
    Cited by
    60601-plasmid-map-sequence-id-97111

    Enlarge

Showing: 3141 - 3160 of 14223 results