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pXPR_003 sgMYC guide 2

Plasmid

#193597

Purpose

cMYC knockout

Depositor

William Hahn

Article

Hagel et al Cancer Res. 2022 Dec 22:CAN-22-2245. doi

Insert

sgMYC guide 2 (MYC Human)

Use

Lentiviral

Available Since

Jan. 3, 2023

Availability

Academic Institutions and Nonprofits only

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pXPR_003 sgAP2M1 guide 1

Plasmid

#193600

Purpose

AP2M1 knockout

Depositor

William Hahn

Article

Hagel et al Cancer Res. 2022 Dec 22:CAN-22-2245. doi

Insert

sgAP2M1 guide 1 (AP2M1 Human)

Use

Lentiviral

Available Since

Jan. 3, 2023

Availability

Academic Institutions and Nonprofits only

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pXPR_003 sgRELA guide 1

Plasmid

#193591

Purpose

RELA knockout

Depositor

William Hahn

Article

Hagel et al Cancer Res. 2022 Dec 22:CAN-22-2245. doi

Insert

sgRELA guide 1 (RELA Human)

Use

Lentiviral

Available Since

Jan. 3, 2023

Availability

Academic Institutions and Nonprofits only

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pSiMPls_N + pSiMPls_C

Plasmid

#167203

Purpose

SiMPl plasmid pair (pSiMPls) for use with spectinomycin/streptomycin

Depositor

Barbara Di Ventura

Article

Palanisamy et al ACS Omega. 2021 May 21;6(22):14148-14153

Inserts

EGFP
SmR 1-75 + gp41-1 N-intein
mRuby3
gp41-1 C-intein + SmR 76-263

Expression

Bacterial

Promoter

AmpR, CAT, pBAD, and pTrc

Available Since

Oct. 6, 2022

Availability

Academic Institutions and Nonprofits only

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pCfB9337 (pgRNA_IV-1_NatMX)

Plasmid

#161590

Purpose

EasyClone-MarkerFree guiding RNA vector to direct Cas9 to cut at site IV-1

Depositor

Irina Borodina

Article

Babaei et al FEMS Yeast Res. 2021 May 11;21(4). pii:

Insert

guiding RNA

Use

CRISPR and Synthetic Biology

Expression

Yeast

Available Since

Aug. 9, 2022

Availability

Academic Institutions and Nonprofits only

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pBM13-sd4U

Plasmid

#179691

Purpose

Bacterial expression of base editor

Depositor

David Liu

Article

Mok et al Nat Biotechnol. 2022 Sep;40(9):1378-1387

Insert

[sd4U] UGI-TALE3-DddA-G1397-N (Q1310R + S1330I + T1380I)

Expression

Bacterial

Mutation

Q1310R, S1330I, T1380I

Available Since

April 4, 2022

Availability

Academic Institutions and Nonprofits only

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pHR-HOXD13-IDR-YFP-NLS

Plasmid

#145277

Purpose

Mammalian Expression of Nuclear HOXD13 IDR-YFP with SV40 NLS

Depositor

Denes Hnisz

Article

Basu et al Cell. 2020 May 28;181(5):1062-1079.e30.

Insert

HOXD13-IDR (HOXD13 Human)

Expression

Mammalian

Available Since

Sept. 23, 2020

Availability

Academic Institutions and Nonprofits only

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pGAL4-DBD-HOXA13-58A-IDR

Plasmid

#145238

Purpose

Expresses fusion of GAL4 DNA-binding domain and HOXA13-58A IDR

Depositor

Denes Hnisz

Article

Basu et al Cell. 2020 May 28;181(5):1062-1079.e30.

Insert

HOXA13-58A-IDR (HOXA13 Human)

Expression

Mammalian

Mutation

A-repeats deleted

Available Since

Sept. 21, 2020

Availability

Academic Institutions and Nonprofits only

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pYFP-x-1

Plasmid

#140142

Purpose

Evaluating information transmission of a genetic sensor.

Depositor

Drew Tack

Article

Sarkar et al Commun Biol. 2020 Apr 30;3(1):203. doi:

Insert

lacY (lacY )

Expression

Bacterial

Mutation

D37, I38, and N39 are changed to nonsense (stop) …

Available Since

April 29, 2020

Availability

Academic Institutions and Nonprofits only

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pNLRP11minor.MYC

Plasmid

#131195

Purpose

Mammalian expression of human NLRP11 188A

Depositor

Thomas Kufer

Article

Ellwanger et al J Biol Chem. 2018 Jan 4. pii: RA117.0001

Insert

NLRP11 188A (NLRP11 Human)

Tags

myc

Expression

Mammalian

Mutation

188A

Promoter

pCMV

Available Since

Sept. 4, 2019

Availability

Academic Institutions and Nonprofits only

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pEPOR0SP0011

Plasmid

#117530

Purpose

Level0 Golden Gate part: CDS, Sp-xCas9 3.7 (no stop codon)

Depositor

Nicola Patron

Article

Raitskin et al PLoS One. 2019 Feb 27;14(2):e0211598. do

Insert

Sp-xCas9 3.7 (no stop codon)

Use

CRISPR

Mutation

A262T, R324L, S409I, E480K, E543D, M694I, E1219V

Available Since

Nov. 7, 2018

Availability

Academic Institutions and Nonprofits only

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pcDNA3.1-R1ACR_741-EYFP

Plasmid

#103961

Purpose

Highly efficient anion channelrhodopsin from Rhodomonas sp. (CCMP768) for expression in mammalian cells

Depositor

John Spudich

Article

Govorunova et al Sci Rep. 2017 Mar 3;7:43358. doi: 10.103

Insert

Anion channelrhodopsin R1ACR_741

Tags

EYFP

Expression

Mammalian

Mutation

human codon optimized

Promoter

CMV

Available Since

Nov. 30, 2017

Availability

Academic Institutions and Nonprofits only

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pONL-N1_LAMP1

Plasmid

#65686

Purpose

Expresses LAMP1-ONL in mammalian cells

Depositor

Yasushi Okada

Article

Takai et al Proc Natl Acad Sci U S A. 2015 Apr 7;112

Insert

LAMP1

Use

Luciferase

Tags

ONL

Expression

Mammalian

Promoter

CMV

Available Since

Jan. 15, 2016

Availability

Academic Institutions and Nonprofits only
pGL4.23 C3_19

Plasmid

#60302

Purpose

This plasmid contains a human pancreatic islet active enhancer, a minimal promoter and a luc2 gene.

Depositor

Jorge Ferrer

Article

Pasquali et al Nat Genet. 2014 Feb;46(2):136-43. doi: 1

Insert

THADA enhancer (THADA Human)

Use

Luciferase

Tags

Luciferase

Expression

Mammalian

Available Since

Dec. 2, 2014

Availability

Academic Institutions and Nonprofits only
pattB-synaptobrevin-5-G4BDMD-QFADM1-hsp70

Plasmid

#46113

Depositor

Christopher Potter

Article

Riabinina et al Nat Methods. 2015 Jan 12. doi: 10.1038/n

Insert

G4BDMD-QFADM1

Expression

Insect

Available Since

Oct. 1, 2013

Availability

Academic Institutions and Nonprofits only

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FUW-[EF1α-CD19 CD28Z]

Plasmid

#218338

Purpose

Lentiviral transfer plasmids for mammalian expression CD19 CAR

Depositor

Haoyi Wang

Article

Zhang et al Cell. 2024 Jul 11;187(14):3741-3760.e30.

Insert

CD19 CAR mammalian expression cassette

Use

Lentiviral

Expression

Mammalian

Available Since

Oct. 23, 2024

Availability

Academic Institutions and Nonprofits only

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FUW-[EF1α-HER1 CD28Z]

Plasmid

#218339

Purpose

Lentiviral transfer plasmids for mammalian expression HER1 CAR

Depositor

Haoyi Wang

Article

Zhang et al Cell. 2024 Jul 11;187(14):3741-3760.e30.

Insert

HER1 CAR mammalian expression cassette

Use

Lentiviral

Expression

Mammalian

Available Since

Oct. 23, 2024

Availability

Academic Institutions and Nonprofits only

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pRRLSIN-[EF1α-CD19 4-1BB]

Plasmid

#218340

Purpose

Lentiviral transfer plasmids for mammalian expression CD19 CAR

Depositor

Haoyi Wang

Article

Zhang et al Cell. 2024 Jul 11;187(14):3741-3760.e30.

Insert

CD19 CAR mammalian expression cassette

Use

Lentiviral

Expression

Mammalian

Available Since

Oct. 23, 2024

Availability

Academic Institutions and Nonprofits only

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pQLL-Tc1-8B_DR[EF1α-copGFP]

Plasmid

#218327

Purpose

Donor plasmid of Tc1-8B_DR Transposon with copGFP mammalian expression cassette

Depositor

Haoyi Wang

Article

Zhang et al Cell. 2024 Jul 11;187(14):3741-3760.e30.

Insert

copGFP mammalian expression cassette

Expression

Mammalian

Available Since

Oct. 23, 2024

Availability

Academic Institutions and Nonprofits only

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PGK-LCB1_v1.3-NotchGal4VP64-T2A-mCherry (pZYW046)

Plasmid

#194229

Purpose

Lentiviral expression vector expressing LCB1 SynNotch and mCherry transduction marker

Depositor

Hana El-Samad

Article

Weinberg et al bioRxiv [Preprint]. 2021 Apr 20:2021.04.

Insert

PGK-LCB1-SynNotch-T2A-mCherry

Use

Lentiviral

Promoter

PGK

Available Since

Sept. 10, 2024

Availability

Academic Institutions and Nonprofits only

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