We narrowed to 10,133 results for: ada

1. pRS415_pGal-nCas9(H840A)

   Plasmid

   #79616

   Purpose

   Expresses nCas9(H840A) in yeast cells

   Depositor

   Akihiko Kondo

   Article

   Nishida et al Science. 2016 Aug 4. pii: aaf8729.

   Insert

   SpCas9
   

   Expression

   Yeast

   Mutation

   H840A for nickase Cas9

   Promoter

   pGal1

   Available Since

   Sept. 2, 2016

   Availability

   Academic Institutions and Nonprofits only

   

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2. px458 EQR

   Plasmid

   #101731

   Purpose

   Expresses a sgRNA and a Cas9 EQR variant that recognizes "NGAG" PAM motifs

   Depositor

   Andrew Holland

   Article

   Lambrus et al Methods Cell Biol. 2018;144:107-135. doi

   Insert

   SpCas9 EQR
   

   Tags

   3xFLAG-NLS and NLS

   Expression

   Mammalian

   Mutation

   EQR (D1135E, R1335Q, and T1337R mutations in Cas9)

   Available Since

   Oct. 17, 2017

   Availability

   Academic Institutions and Nonprofits only

   

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3. pEBG-TRAF2 (GST)

   Plasmid

   #21586

   Depositor

   John Kyriakis

   Article

   Chadee et al Mol Cell Biol. 2002 Feb . 22(3):737-49.

   Insert

   TNF receptor-associated factor 2 (TRAF2 Human)
   

   Tags

   GST

   Expression

   Mammalian

   Available Since

   Nov. 24, 2009

   Availability

   Academic Institutions and Nonprofits only

4. pPLtetO-ClpX

   Plasmid

   #89657

   Purpose

   Plasmid for titrating synthesis of ClpX with doxycycline

   Depositor

   Uwe Sauer

   Article

   Sekar et al Mol Syst Biol. 2018 Nov 5;14(11):e8623.

   Insert

   clpX
   

   Use

   Synthetic Biology

   Available Since

   May 8, 2018

   Availability

   Academic Institutions and Nonprofits only

   

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5. EMTB-mTurquoise2

   Plasmid

   #137803

   Purpose

   Visualization of microtubules

   Depositor

   Dorus Gadella

   Article

   Chertkova et al bioRxiv (2020) 160374; doi: https://doi.

   Insert

   Ensconsin microtubule binding domain
   

   Expression

   Mammalian

   Promoter

   CMV

   Available Since

   March 30, 2020

   Availability

   Academic Institutions and Nonprofits only

   

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6. pEGFP-IREShygro2

   Plasmid

   #153966

   Purpose

   Expresses EGFP in mammalian cells

   Depositor

   Hiroyuki Konishi

   Article

   Hyodo et al Cell Rep. 2020 Jan 28;30(4):1195-1207.e7

   Insert

   EGFP
   

   Tags

   N/A

   Expression

   Mammalian

   Mutation

   No mutation

   Promoter

   CMV promoter

   Available Since

   July 22, 2020

   Availability

   Academic Institutions and Nonprofits only

   

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7. pJW1354

   Plasmid

   #69490

   Purpose

   Gly-Ser-4xGly linker-degron-TEV-3xFLAG with 40 bp nhr-25 homology arms

   Depositor

   Jordan Ward

   Article

   Zhang et al Development. 2015 Nov 9. pii: dev.129635

   Insert

   GSGGGG spacer-Degron-TEV protease site-3x FLAG epitope
   

   Use

   CRISPR; Template to generate cassettes for -media…

   Available Since

   Sept. 30, 2015

   Availability

   Academic Institutions and Nonprofits only

   

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8. pLJM1 Flag Raptor HRas25

   Plasmid

   #26637

   Depositor

   David Sabatini

   Article

   Sancak et al Cell. 2010 Apr 16. 141(2):290-303.

   Insert

   raptor-HRas25 (RPTOR Human)
   

   Use

   Lentiviral

   Tags

   Flag

   Expression

   Mammalian

   Available Since

   Nov. 16, 2010

   Availability

   Academic Institutions and Nonprofits only

9. px458_2A_GFP_sgRNA_ZNF598

   Plasmid

   #127121

   Depositor

   Thomas Tuschl

   Article

   Meyer et al Mol Cell. 2020 Mar 19;77(6):1193-1205.e5

   Insert

   gRNA ZNF598 (ZNF598 Human)
   

   Use

   CRISPR

   Available Since

   July 26, 2019

   Availability

   Academic Institutions and Nonprofits only

   

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10. pCDH-EF1a-mTagBFP-T2A-TetOn3G

    Plasmid

    #179888

    Purpose

    Expresses TetOn3G transcription factor selectable by hygromycin resistance (Lentiviral vector)

    Depositor

    Patrick Caswell

    Article

    Gemperle et al Elife. 2022 Jun 16;11. pii: 76651. doi:

    Insert

    TetOn3G
    

    Use

    Lentiviral

    Tags

    mTagBFP

    Expression

    Bacterial and Mammalian

    Promoter

    EF1a

    Available Since

    May 31, 2022

    Availability

    Academic Institutions and Nonprofits only

    

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11. px459 VRER

    Plasmid

    #101716

    Purpose

    sgRNA/SpCas9 expression plasmid with Cas9 VRER mutations (NGCG PAM)

    Depositor

    Andrew Holland

    Article

    Lambrus et al Methods Cell Biol. 2018;144:107-135. doi

    Insert

    SpCas9 VRER
    

    Tags

    3xFLAG-NLS and NLS

    Expression

    Mammalian

    Mutation

    VRER (D1135V, G1218R, R1335E and T1337R)

    Available Since

    Oct. 20, 2017

    Availability

    Academic Institutions and Nonprofits only

    

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12. pCfuNca-Plastid

    Plasmid

    #190818

    Purpose

    Plastid targeting GFP with ble resistant gene for zeocin

    Depositor

    Peter Kroth

    Article

    Sprecher et al Algal Research. 2023 May; Vol 72: 103136

    Inserts

    Diatom plastid targeted GFP
    Bleomycin resistance gene
    

    Expression

    Bacterial

    Promoter

    Cylindrotheca fusiformis fcp and Nitzschia captiv…

    Available Since

    Aug. 14, 2023

    Availability

    Academic Institutions and Nonprofits only

    

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13. Clathrin-LCa-ECFP

    Plasmid

    #21742

    Depositor

    Xiaowei Zhuang

    Article

    Chen et al Proc Natl Acad Sci U S A. 2008 Aug 19. 1

    Insert

    Clathrin, light polypeptide (LCa) (Clta Mouse)
    

    Tags

    ECFP

    Expression

    Mammalian

    Available Since

    Aug. 5, 2009

    Availability

    Academic Institutions and Nonprofits only

14. pEF6 rglut1 flag complete

    Plasmid

    #89571

    Purpose

    rglut1 flag complete

    Depositor

    Jeffrey Rathmell

    Article

    Wieman et al Biochem J. 2009 Mar 1;418(2):345-67. doi

    Insert

    rglut1 flag complete (Slc2a1 Rat)
    

    Tags

    Exo Facial

    Expression

    Mammalian

    Promoter

    EF-1a

    Available Since

    May 22, 2017

    Availability

    Academic Institutions and Nonprofits only

    

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15. pMK401 (Hygro-P2A-mAID-Nluc)

    Plasmid

    #121196

    Purpose

    N-terminal tagging

    Depositor

    Masato Kanemaki

    Article

    Yesbolatova et al Methods. 2019 Apr 23. pii: S1046-2023(18

    Insert

    Hygro-P2A-mAID-Nluc
    

    Use

    CRISPR

    Available Since

    Feb. 13, 2019

    Availability

    Academic Institutions and Nonprofits only

    

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16. pET22b-GFP-CPDSalI

    Plasmid

    #38257

    Depositor

    Matthew Bogyo, Aimee Shen

    Article

    Shen et al PLoS One. 2009 Dec 2;4(12):e8119.

    Insert

    eGFP
    

    Tags

    6xHis and Vibrio cholerae MARTX toxin cysteine pr…

    Expression

    Bacterial

    Mutation

    cloned from pEGFPN3 (Clonetech)

    Promoter

    T7

    Available Since

    July 20, 2012

    Availability

    Academic Institutions and Nonprofits only

17. pAAV-CAG-mNeptune2.5-P2A-GFP

    Plasmid

    #197156

    Purpose

    Expresses the protein of mNeptune2.5-P2A-GFP in mammalian cells

    Depositor

    Kiryl Piatkevich

    Article

    Zhang et al Nat Methods. 2023 Sep 4. doi: 10.1038/s4

    Insert

    mNeptune2.5-P2A-GFP
    

    Use

    AAV

    Available Since

    March 10, 2023

    Availability

    Academic Institutions and Nonprofits only

    

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18. pTK332

    Plasmid

    #59573

    Purpose

    SIN SP6 P1234 (nsP2 P726S) SGP(14) 2xK-turn Kozak MS2-CNOT7 (SacI mutated)-P2A-EBFP2-4xFF5

    Depositor

    Ron Weiss

    Article

    Wroblewska et al Nat Biotechnol. 2015 Aug 3. doi: 10.1038

    Insert

    SGP(14) 2xK-turn Kozak MS2-CNOT7 (SacI mutated)-P2A-EBFP2-4xFF5
    

    Available Since

    Aug. 3, 2015

    Availability

    Academic Institutions and Nonprofits only

    

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19. pspCas9-3'UTR-Tetra-com-vector

    Plasmid

    #132553

    Purpose

    Expresses spCas9 and sgRNA scaffold with com aptamer replacing the Tetraloop

    Depositor

    Baisong Lu

    Article

    Lu et al CRISPR J. 2021 Mar 16. doi: 10.1089/cris

    Inserts

    Cas9
    sgRNA scaffold with com replacing the Tetraloop
    

    Expression

    Mammalian

    Available Since

    March 22, 2021

    Availability

    Academic Institutions and Nonprofits only

    

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20. pRK-flag-USP19 1-1290

    Plasmid

    #78579

    Purpose

    Expression flag-USP19 1-1290 in mammalian cells

    Depositor

    Yihong Ye

    Article

    Lee et al Nat Cell Biol. 2016 Jul;18(7):765-76. do

    Insert

    flag-USP19 1-1290 (USP19 Human)
    

    Tags

    flag

    Expression

    Mammalian

    Mutation

    mutate 1291 residue to stop condon

    Promoter

    CMV

    Available Since

    June 22, 2016

    Availability

    Academic Institutions and Nonprofits only