We narrowed to 5,304 results for: pcDNA3.1(+)

1. tCRMod-GGSGGS9

   Plasmid

   #111762

   Purpose

   Expresses soluble Clover-mRuby2 tension sensor module with 9-repeat GGSGGS extensible domain

   Depositor

   Brenton Hoffman

   Article

   LaCroix et al eLife. 2018 Jul 19;7. pii: 33927. doi: 1

   Insert

   Clover-GGSGGS9-mRuby2 fusion
   

   Use

   Tags

   Expression

   Mammalian

   Mutation

   Residues 1-228 of Clover, residues 1-3 deleted fr…

   Promoter

   CMV

   Available Since

   July 5, 2018

   Availability

   Academic Institutions and Nonprofits only

   

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2. pPD005 pcDNA Golden Gate

   Plasmid

   #138749

   Purpose

   Vector used to express most COMET components, modified from pcDNA3.1/HygroR(+) for BsaI/BpiI Golden Gate compatibility and to lack HygroR.

   Depositor

   Joshua Leonard

   Article

   Donahue et al Nat Commun. 2020 Feb 7;11(1):779. doi: 1

   Type

   Empty backbone

   Use

   Synthetic Biology

   Tags

   Expression

   Mammalian

   Mutation

   Promoter

   Available Since

   Jan. 6, 2021

   Availability

   Academic Institutions and Nonprofits only

   

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3. ER2-mGreenLantern

   Plasmid

   #186548

   Purpose

   Localization and retention of mGreenLantern fluorescent protein in the ER; improved targeting efficiency

   Depositor

   Gregory Petsko

   Article

   Campbell et al Nat Methods. 2022 Nov 7. pii: 10.1038/s4

   Insert

   mGreenLantern
   

   Use

   Tags

   Calreticulin and KDEL

   Expression

   Mammalian

   Mutation

   Clover-F64L/S72A/E124V/N149K/I167T/S175G/A206K/L2…

   Promoter

   CMV

   Available Since

   Nov. 8, 2022

   Availability

   Academic Institutions and Nonprofits only

   

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4. ER2-LSSmGFP

   Plasmid

   #186538

   Purpose

   Localization and retention of LSSmGFP fluorescent protein (ex/em = 400/510 nm) in the ER; improved targeting efficiency

   Depositor

   Gregory Petsko

   Article

   Campbell et al Nat Methods. 2022 Nov 7. pii: 10.1038/s4

   Insert

   LSSmGFP
   

   Use

   Tags

   Calreticulin and KDEL

   Expression

   Mammalian

   Mutation

   hfYFP-T43S/G65S/L68Q/H77N/K140N/Y203I/V206K

   Promoter

   CMV

   Available Since

   Nov. 8, 2022

   Availability

   Academic Institutions and Nonprofits only

   

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5. Fra-1

   Plasmid

   #187906

   Purpose

   Expresses Fra-1 in transfected cells

   Depositor

   Jennifer Mitchell

   Article

   Mitchell et al Endocrinology. 2005 Apr;146(4):2048-54.

   Insert

   Fra1 (Fosl1 Rat)
   

   Use

   Tags

   Expression

   Mammalian

   Mutation

   Promoter

   Available Since

   Oct. 25, 2022

   Availability

   Academic Institutions and Nonprofits only

   

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6. pcDNA-ALFA-tag-Gal4-VP16

   Plasmid

   #199465

   Purpose

   Expression of the Gal4-ALFA-tag-VP16 synthetic transcription factor containing the ALFA-tag sequence in mammalian cells.

   Depositor

   Vladislav Verkhusha

   Article

   Chernov et al Int J Mol Sci. 2023 Mar 30;24(7):6526. d

   Insert

   Gal4-ALFA-tag-VP16
   

   Use

   Tags

   Expression

   Mammalian

   Mutation

   Promoter

   Available Since

   June 26, 2023

   Availability

   Academic Institutions and Nonprofits only

   

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7. LacI INCENP

   Plasmid

   #45236

   Purpose

   Target INCENP to lac operator sequence

   Depositor

   Michael Lampson

   Article

   Wang et al J Cell Biol. 2011 Aug 22;194(4):539-49.

   Insert

   LacI, INCENP-Δcen, mCherry (lacI )
   

   Use

   Tags

   LacI (lac repressor protein) and mCherry

   Expression

   Mammalian

   Mutation

   CEN box deleted from INCENP (aa1-46)

   Promoter

   t7

   Available Since

   Sept. 4, 2014

   Availability

   Academic Institutions and Nonprofits only

   

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8. pVH233-Tier1-PmPGK1-eYFP

   Plasmid

   #169522

   Purpose

   Tier-1 vector encoding PmPGK1-driven eYFP expression (PmPGK1-eYFP-pA).

   Depositor

   Martin Fussenegger

   Article

   Haellman et al Metab Eng. 2021 Jul;66:41-50. doi: 10.10

   Insert

   PPGK-driven enhanced yellow fluorescent protein
   

   Use

   Tags

   Expression

   Mammalian

   Mutation

   Promoter

   PPGK

   Available Since

   June 16, 2022

   Availability

   Academic Institutions and Nonprofits only

   

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9. pEQ1007

   Plasmid

   #83938

   Purpose

   express human cytomegalovirus IRS1 with a C-terminal 6X his tag in eukaryotic cells

   Depositor

   Adam Geballe

   Article

   Hakki et al J Virol. 2006 Dec;80(23):11817-26. Epub

   Insert

   IRS1
   

   Use

   Tags

   6xHIS

   Expression

   Mammalian

   Mutation

   R833L in IRS1 (please see depositor comment)

   Promoter

   CMV

   Available Since

   Nov. 4, 2016

   Availability

   Academic Institutions and Nonprofits only

   

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10. TR therapy reporter

    Plasmid

    #179083

    Purpose

    Fluorescent reporter for alternative splicing

    Depositor

    Thurman Wheeler

    Article

    Hu et al Nat Commun. 2018 Dec 7;9(1):5227. doi: 1

    Inserts

    DsRed1
    EGFP
    

    Use

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    Available Since

    Feb. 15, 2022

    Availability

    Academic Institutions and Nonprofits only

    

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11. pcPylRS(Y384F Y306A)

    Plasmid

    #196486

    Purpose

    To express Methanosarcina mazei PylRS(Y384F Y306A) and allow UAG codon to be translated into Z-Lys or Azido-Z-Lys when used together with the pU6tRNAPyl plasmid.

    Depositor

    Kensaku Sakamoto

    Article

    Hino et al Methods Mol Biol. 2012;794:215-28. doi:

    Insert

    pyrrolysyl-tRNA synthetase variant with Y384F Y306A mutations
    

    Use

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    CMV

    Available Since

    March 6, 2023

    Availability

    Academic Institutions and Nonprofits only

    

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12. pcDNA_RFP-SZ2

    Plasmid

    #124936

    Purpose

    Expression of RFP-SZ2

    Depositor

    Matthew Good, Daniel Hammer, Benjamin Schuster

    Article

    Schuster et al Nat Commun. 2018 Jul 30;9(1):2985. doi:

    Insert

    RFP-SYNZIP2
    

    Use

    Tags

    mCherry

    Expression

    Mammalian

    Mutation

    SZ2 domain

    Promoter

    Available Since

    May 29, 2019

    Availability

    Academic Institutions and Nonprofits only

    

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13. pZ143-pcDNA3-BvCas12b

    Plasmid

    #122445

    Purpose

    Expresses BvCas12b in mammalian cells

    Depositor

    Feng Zhang

    Article

    Strecker et al Nat Commun. 2019 Jan 22;10(1):212. doi:

    Insert

    CMV-NLS-BvCas12b-NLS-3xHA
    

    Use

    Tags

    3XHA

    Expression

    Mammalian

    Mutation

    Promoter

    Available Since

    Feb. 21, 2019

    Availability

    Academic Institutions and Nonprofits only

    

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14. pmiRFP670nano3-NbGFP

    Plasmid

    #184686

    Purpose

    Nanobody to GFP labelled with near-infrared fluorescent protein miRFP670nano3

    Depositor

    Vladislav Verkhusha

    Article

    Oliinyk et al Nat Methods. 2022 May 23. pii: 10.1038/s

    Insert

    miRFP670nano3-NbGFP
    

    Use

    Tags

    miRFP670nano3

    Expression

    Mammalian

    Mutation

    Promoter

    CMV

    Available Since

    Sept. 9, 2022

    Availability

    Academic Institutions and Nonprofits only

    

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15. pcDNA3-ClopHensor-H148G-V224L

    Plasmid

    #46366

    Depositor

    Daniele Arosio

    Article

    Mukhtarov et al Front Mol Neurosci. 2013 Apr 18;6:9. doi

    Insert

    ClopHensor-H148G-V224L
    

    Use

    Tags

    Strep-II

    Expression

    Mammalian

    Mutation

    E2GFP-H148G-V224L is EGFP-T203Y-H148G-V224L

    Promoter

    Available Since

    Aug. 12, 2013

    Availability

    Academic Institutions and Nonprofits only

16. pZ147-BvCas12b-sgRNA-scaffold

    Plasmid

    #122447

    Purpose

    Expresses sgRNA for BvCas12b in mammalian cells

    Depositor

    Feng Zhang

    Article

    Strecker et al Nat Commun. 2019 Jan 22;10(1):212. doi:

    Insert

    U6-BvCas12b-sgRNA-scaffold, CMV-mCherry
    

    Use

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    Available Since

    Feb. 21, 2019

    Availability

    Academic Institutions and Nonprofits only

    

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17. Rtn3c-mEmerald

    Plasmid

    #186963

    Purpose

    Express Rtn3c with C-terminus mEmerald

    Depositor

    Ke Xu

    Article

    Wang et al Proc Natl Acad Sci U S A. 2022 May 3;119

    Insert

    RTN3C (Rtn3 Mouse)
    

    Use

    Tags

    mEmerald

    Expression

    Mammalian

    Mutation

    Promoter

    CMV Promoter

    Available Since

    Sept. 22, 2022

    Availability

    Academic Institutions and Nonprofits only

    

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18. TNXXL-CTS20

    Plasmid

    #48342

    Purpose

    FRET reporter for Ca2+ inside primary cilia

    Depositor

    Takanari Inoue

    Article

    Su et al Nat Methods. 2013 Sep 22. doi: 10.1038/n

    Insert

    CTS20
    

    Use

    Tags

    TN-XXL

    Expression

    Mammalian

    Mutation

    Promoter

    Available Since

    Nov. 14, 2013

    Availability

    Academic Institutions and Nonprofits only

19. tCRMod-GGSGGS7

    Plasmid

    #111761

    Purpose

    Expresses soluble Clover-mRuby2 tension sensor module with 7-repeat GGSGGS extensible domain

    Depositor

    Brenton Hoffman

    Article

    LaCroix et al eLife. 2018 Jul 19;7. pii: 33927. doi: 1

    Insert

    Clover-GGSGGS7-mRuby2 fusion
    

    Use

    Tags

    Expression

    Mammalian

    Mutation

    Residues 1-228 of Clover, residues 1-3 deleted fr…

    Promoter

    CMV

    Available Since

    July 5, 2018

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

20. tCRMod-GGSGGS5

    Plasmid

    #111760

    Purpose

    Expresses soluble Clover-mRuby2 tension sensor module with 5-repeat GGSGGS extensible domain

    Depositor

    Brenton Hoffman

    Article

    LaCroix et al eLife. 2018 Jul 19;7. pii: 33927. doi: 1

    Insert

    Clover-GGSGGS5-mRuby2 fusion
    

    Use

    Tags

    Expression

    Mammalian

    Mutation

    Residues 1-228 of Clover, residues 1-3 deleted fr…

    Promoter

    CMV

    Available Since

    July 5, 2018

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge