We narrowed to 31,032 results for: uros

1. pRC11-U6-DR-crRNA-BsmbI(x2); EFS-Puro-WPRE

   Plasmid

   #123360

   Purpose

   Lentiviral vector with empty U6 cassette containing LbCpf1 direct repeat, and constitutive expression of puromycin resistance.

   Depositor

   Sidi Chen

   Article

   Chow et al Nat Methods. 2019 May;16(5):405-408. doi

   Insert

   Empty LbCpf1 crRNA cloning cassette
   

   Use

   CRISPR and Lentiviral

   Tags

   Expression

   Mammalian

   Mutation

   Promoter

   U6

   Available since

   May 14, 2019

   Availability

   Academic Institutions and Nonprofits only

   

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2. Direct-seq_lentiGuide-Puro-tRNA-Loop2-8A8G

   Plasmid

   #157986

   Purpose

   Expresses programmed gRNA scaffold from U6 promoter. Programmed gRNA scaffold for streamlined scRNA-seq in CRISPR screen (Direct-seq). 3rd generation lentiviral backbone.

   Depositor

   Lijia Ma

   Article

   Song et al Genome Biol. 2020 Jun 8;21(1):136. doi:

   Insert

   S. pyogenes sgRNA cassette (tRNA-Loop2-8A8G)
   

   Use

   CRISPR and Lentiviral

   Tags

   Expression

   Mammalian

   Mutation

   Promoter

   hU6

   Available since

   May 10, 2021

   Availability

   Academic Institutions and Nonprofits only

   

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3. pLenti CMV-MRLC1-mRuby2-IRES-PuroR

   Plasmid

   #103031

   Purpose

   Lentiviral expression of mRuby2-tagged MRLC with puromycin selection

   Depositor

   Ghassan Mouneimne

   Article

   Padilla-Rodriguez et al Nat Commun. 2018 Jul 30;9(1):2980. doi:

   Insert

   MRLC1 (MYL9 Human)
   

   Use

   Lentiviral

   Tags

   mRuby2

   Expression

   Mutation

   Promoter

   CMV immediate early

   Available since

   May 22, 2018

   Availability

   Academic Institutions and Nonprofits only

   

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4. pLKO.1-puro-U6-SpyCas9-TLR-MCV1-sgRNA2

   Plasmid

   #117406

   Purpose

   SpyCas9 sgRNA 2 targeting TLR2.0

   Depositor

   Erik Sontheimer

   Article

   Iyer et al CRISPR J. 2022 Oct;5(5):685-701. doi: 10

   Insert

   SpyCas9 sgRNA targeting TLR 2.0
   

   Use

   Lentiviral

   Tags

   Expression

   Mammalian

   Mutation

   Promoter

   U6

   Available since

   Dec. 5, 2019

   Availability

   Academic Institutions and Nonprofits only

   

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5. pLenti PGK mKate2-OGT 5N5A (Puro)

   Plasmid

   #154293

   Purpose

   Lentiviral vector encoding full length mKate2-2xFLAG-OGT (full length human O-GlcNAc transferase, 5N5A variant, no HCF-1 cleavage)

   Depositor

   Suzanne Walker

   Article

   Levine et al Proc Natl Acad Sci U S A. 2021 Jan 26;11

   Insert

   O-GlcNAc Transferase (OGT Human)
   

   Use

   Lentiviral

   Tags

   2x FLAG and mKate2

   Expression

   Mammalian

   Mutation

   N322A, N356A, N390A, N424A, N458A mutations; HCF-…

   Promoter

   PGK

   Available since

   Nov. 16, 2020

   Availability

   Academic Institutions and Nonprofits only

   

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6. pLEX-uORF-HA-birA*-STOP-IRES-Puro

   Plasmid

   #120558

   Purpose

   Expresses control HA-birA* in mammalian cells & for virus production

   Depositor

   Paul Khavari

   Article

   Kovalski et al Mol Cell. 2019 Jan 4. pii: S1097-2765(18

   Insert

   birA
   

   Use

   Lentiviral

   Tags

   HA

   Expression

   Mammalian

   Mutation

   R118G

   Promoter

   CMV

   Available since

   Jan. 30, 2019

   Availability

   Academic Institutions and Nonprofits only

   

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7. pLenti-EF1a-CDK6-mutPAM-PGK-puro

   Plasmid

   #187638

   Purpose

   sg resistant CDK6 ORF

   Depositor

   William Kaelin

   Article

   Nicholson et al Sci Signal. 2019 Oct 1;12(601). pii: 12/

   Insert

   CDK6 (CDK6 Human)
   

   Use

   Lentiviral

   Tags

   Expression

   Mammalian

   Mutation

   PAM site mutation

   Promoter

   Available since

   Aug. 11, 2023

   Availability

   Academic Institutions and Nonprofits only

   

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8. MSCV-Myc-PGK-Puro-IRES-GFP

   Plasmid

   #75124

   Purpose

   Retroviral expression plasmid encoding wild type murine Myc

   Depositor

   Christopher Vakoc

   Article

   Hohmann et al Nat Chem Biol. 2016 Jul 4. doi: 10.1038/

   Insert

   myelocytomatosis oncogene (Myc Mouse)
   

   Use

   Retroviral

   Tags

   None

   Expression

   Mutation

   Promoter

   Available since

   May 20, 2016

   Availability

   Academic Institutions and Nonprofits only

9. N113 nLV EF-1a-Gal4-Stop-PGK-Puro

   Plasmid

   #44176

   Depositor

   Jerry Crabtree

   Article

   Hathaway et al Cell. 2012 Jun 22;149(7):1447-60. doi: 1

   Insert

   Gal4/DBD (1-147)
   

   Use

   Lentiviral

   Tags

   Expression

   Mutation

   Promoter

   EF-1a

   Available since

   June 5, 2013

   Availability

   Academic Institutions and Nonprofits only

   

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10. pCT-CMV-UBE3B(C1036A)-copGFP-Puro

    Plasmid

    #107344

    Purpose

    Expresses UBE3B mutant C1036A fused to copGFP in mammalian cells.

    Depositor

    Robert Sobol

    Article

    Braganza et al J Biol Chem. 2016 Dec 21. pii: jbc.M116.

    Insert

    UBE3B(C1036A)copGFP (UBE3B Human)
    

    Use

    Lentiviral

    Tags

    copGFP

    Expression

    Mammalian

    Mutation

    mutation C1036A

    Promoter

    CMV

    Available since

    April 19, 2018

    Availability

    Industry, Academic Institutions, and Nonprofits

    

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11. pLenti CMV Puro DEST (w118-1) MXRA7

    Plasmid

    #107500

    Purpose

    Expresses MXRA7, puromycin resistant

    Depositor

    Alejandro Gutierrez

    Article

    Aries et al J Exp Med. 2018 Nov 7. pii: jem.20180570

    Insert

    MXRA7 (codon optimized) (MXRA7 Human)
    

    Use

    Lentiviral

    Tags

    Expression

    Mammalian

    Mutation

    Codon optimized sequence

    Promoter

    CMV

    Available since

    Nov. 7, 2018

    Availability

    Academic Institutions and Nonprofits only

    

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12. mCherry-ActA-IRES-puromycin-pLVx-EF1a

    Plasmid

    #177433

    Purpose

    To express mCherry fused to ActA mitochondria targeting sequence. Lentiviral vector used to make cell lines expressing this mitochondria landmark.

    Depositor

    David Andrews

    Insert

    ActA tail anchor (actA Listeria monocytogenes)
    

    Use

    Lentiviral

    Tags

    mCherry

    Expression

    Mammalian

    Mutation

    Promoter

    EF1a

    Available since

    Jan. 3, 2023

    Availability

    Academic Institutions and Nonprofits only

    

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13. pBabe-TetCMV-puro-mVenus-PA-Rac1-C450M

    Plasmid

    #22034

    Depositor

    Klaus Hahn

    Article

    Wu et al Nature. 2009 Sep 3. 461(7260):104-8.

    Insert

    mVenus-PA-Rac1-C450M (RAC1 Avena sativa (oat), Human)
    

    Use

    Retroviral

    Tags

    Expression

    Mammalian

    Mutation

    The LOV domain contains the mutation C450M.Rac1 s…

    Promoter

    Available since

    Sept. 29, 2009

    Availability

    Academic Institutions and Nonprofits only

14. pMRX-IRES-puro-3xFLAG-NBR1 deltaUBA

    Plasmid

    #204547

    Purpose

    Expresses 3xFLAG-tagged NBR1 deltaUBA in mammalian cells

    Depositor

    Masaaki Komatsu

    Article

    Kurusu et al Dev Cell. 2023 May 9:S1534-5807(23)00191

    Insert

    NBR1 (NBR1 Human)
    

    Use

    Retroviral

    Tags

    Expression

    Mammalian

    Mutation

    deltaUBA

    Promoter

    Available since

    Aug. 25, 2023

    Availability

    Academic Institutions and Nonprofits only

    

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15. pLenti-PGK-flag-RAP1B-V12-puro

    Plasmid

    #156168

    Purpose

    Lentiviral vector for expression of flag-RAP1B-V12, with puromycin selection

    Depositor

    Roland Friedel

    Article

    Junqueira Alves et al Nat Commun. 2021 Oct 14;12(1):6019. doi:

    Insert

    RAP1B (RAP1B Bovine)
    

    Use

    Lentiviral

    Tags

    2xFLAG

    Expression

    Mammalian

    Mutation

    changed Glycine 12 to Valine

    Promoter

    human PGK

    Available since

    July 26, 2021

    Availability

    Academic Institutions and Nonprofits only

    

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16. pLKO.1-puro-U6-AsCas12a-TLR-MCV1-gRNA

    Plasmid

    #117411

    Purpose

    AsCas12a-gRNA targeting TLR2.0

    Depositor

    Erik Sontheimer

    Article

    Iyer et al CRISPR J. 2022 Oct;5(5):685-701. doi: 10

    Insert

    AsCas12a gRNA targeting TLR 2.0
    

    Use

    Lentiviral

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    U6

    Available since

    Dec. 5, 2019

    Availability

    Academic Institutions and Nonprofits only

    

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17. N111 nLV EF-1a-PGK-Puro Gal4-HP1a FL

    Plasmid

    #43968

    Depositor

    Jerry Crabtree

    Article

    Hathaway et al Cell. 2012 Jun 22;149(7):1447-60. doi: 1

    Insert

    Cbx5 (Cbx5 Mouse)
    

    Use

    Lentiviral

    Tags

    Gal4/DBD

    Expression

    Mutation

    Promoter

    EF-1a

    Available since

    June 5, 2013

    Availability

    Academic Institutions and Nonprofits only

    

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18. pSBbi-moxGFP-P2A-T2A-cMyc-Puro

    Plasmid

    #176892

    Purpose

    Sleeping Beauty transposon-based vector for mammalian polycistronic expression of moxGFP, c-Myc and an additional gene of interest

    Depositor

    Jorge Luis Arias-Arias

    Article

    Acon et al iScience. 2021 Nov 8;24(12):103407. doi:

    Insert

    moxGFP-P2A-T2A-c-Myc
    

    Use

    Transposon

    Tags

    Myc and moxGFP

    Expression

    Mammalian

    Mutation

    Promoter

    EF1a/RPBSA

    Available since

    Nov. 19, 2021

    Availability

    Academic Institutions and Nonprofits only

    

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19. pLenti-BE3RA-P2A-GFP-PGK-Puro

    Plasmid

    #110868

    Purpose

    Lentiviral vector for constitutive expression of BE3RA-P2A-GFP in mammalian cells (codon optimized)

    Depositor

    Lukas Dow

    Article

    Zafra et al Nat Biotechnol. 2018 Jul 3. pii: nbt.419

    Insert

    BE3RA
    

    Use

    Lentiviral

    Tags

    Expression

    Mutation

    D10A

    Promoter

    EF1s

    Available since

    June 27, 2018

    Availability

    Academic Institutions and Nonprofits only

    

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20. pU6-mir144 EF1Alpha-puro-T2A-BFP

    Plasmid

    #164791

    Purpose

    Express miR-144 under the U6 promoter in mammalian cells

    Depositor

    David Bartel

    Article

    Fang et al Mol Cell. 2020 Apr 16;78(2):289-302.e6.

    Inserts

    miR-144 (MIR144 Human)
    puro-T2A-BFP
    

    Use

    Lentiviral

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    EF1Alpha and U6

    Available since

    March 15, 2021

    Availability

    Academic Institutions and Nonprofits only

    

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