We narrowed to 14,579 results for: pET

1. pET51b(+)-NADPH-LUCID-2 (C50 29 nM)

   Plasmid

   #117909

   Purpose

   Plasmid encoding the bacterial expression of the BRET semi-synthetic biosensor measuring the NADPH concentration with a C50 of 29 nM

   Depositor

   Kai Johnsson

   Article

   Yu et al Science. 2018 Sep 14;361(6407):1122-1126

   Insert

   SNAP_Pro30_[cpNLuc23_24]eDHFR(R98A)
   

   Use

   Tags

   His-tag and Strep-tag

   Expression

   Bacterial

   Mutation

   R98A on eDHFR

   Promoter

   T7

   Available since

   Oct. 25, 2018

   Availability

   Academic Institutions and Nonprofits only

   

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2. pcDNA3-AMPKsub-YPet-Kras

   Plasmid

   #84638

   Purpose

   Encodes C-terminal (substrate) fragment of bimolecular AMPK/BRSK activity reporter (bimABKAR); plasma membrane targeted; use in conjunction with pcDNA3-Cerulean-FHA1-NES

   Depositor

   Jin Zhang

   Article

   Depry et al Chem Biol. 2015 Nov 19;22(11):1470-9. do

   Insert

   AMPKsub-YPet-Kras
   

   Use

   Tags

   6xHis, C-terminal targeting sequence from Kras, T…

   Expression

   Mammalian

   Mutation

   Promoter

   CMV

   Available since

   June 10, 2021

   Availability

   Academic Institutions and Nonprofits only

   

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3. pET28-Nsp14-TEV-Strep

   Plasmid

   #154953

   Purpose

   Expresses Nsp14 from SARS-CoV-2 with a C-terminal TEV-protease cleavable Strep tag in E. coli

   Depositor

   Justin Kollman

   Insert

   Nsp14-TEV-Strep (ORF1ab SARS-CoV-2)
   

   Use

   Tags

   TEV-Strep

   Expression

   Bacterial

   Mutation

   Promoter

   T7 Promoter

   Available since

   Aug. 5, 2020

   Availability

   Industry, Academic Institutions, and Nonprofits

   

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4. pET22-Strep-TEV-Nsp10

   Plasmid

   #154958

   Purpose

   Expresses Nsp10 from SARS-CoV-2 with a N-terminal TEV-protease cleavable Strep tag in E. coli

   Depositor

   Justin Kollman

   Insert

   Strep-TEV-Nsp10 (ORF1ab SARS-CoV-2)
   

   Use

   Tags

   Strep-TEV

   Expression

   Bacterial

   Mutation

   Promoter

   T7 Promoter

   Available since

   July 21, 2020

   Availability

   Industry, Academic Institutions, and Nonprofits

   

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5. pET28a-Hisx6-Sirt6-S56A

   Plasmid

   #20278

   Depositor

   Leonard Guarente

   Article

   Liszt et al J Biol Chem. 2005 Jun 3. 280(22):21313-2

   Insert

   Sirt6 (Sirt6 Mouse)
   

   Use

   Tags

   Hisx6

   Expression

   Bacterial

   Mutation

   Serine 56 to Alanine

   Promoter

   Available since

   March 24, 2009

   Availability

   Academic Institutions and Nonprofits only

6. pET21a-hAIM2-PYD-GST

   Plasmid

   #51531

   Purpose

   Express GST-tagged PYD of hAIM2 in bacteria

   Depositor

   Emad Alnemri

   Article

   Fernandes-Alnemri et al Nature. 2009 Mar 26;458(7237):509-13. do

   Insert

   AIM2 (AIM2 Human)
   

   Use

   Tags

   GST and T7

   Expression

   Bacterial

   Mutation

   deletion of amino acids 102-343

   Promoter

   lac operator

   Available since

   March 11, 2014

   Availability

   Academic Institutions and Nonprofits only

   

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7. pSB1C3_mTq2-Tat_YPet-PCP (cy-plasmid)

   Plasmid

   #127636

   Purpose

   Encodes two fluorecent proteins with RNA binding peptides: mTurquoise2-Tat and YPet-PCP. Expression with constitutive E. coli RNAP promoter (J23106), ribozymes RiboJ10 (for YPet) and PlmJ (for mTq2)

   Depositor

   Friedrich Simmel

   Article

   Schwarz-Schilling et al Nano Lett. 2018 Apr 11;18(4):2650-2657.

   Insert

   mTurquoiuse2-Tat, YPet-PCP
   

   Use

   Synthetic Biology

   Tags

   RNA binding peptide: PCP and RNA binding peptide:…

   Expression

   Bacterial

   Mutation

   Promoter

   Available since

   July 29, 2019

   Availability

   Academic Institutions and Nonprofits only

   

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8. pET11a-TAX-L10-STEPtag

   Plasmid

   #159375

   Purpose

   Expresses STEPtag fused to the C-terminus of inactive T. aurantiacus xylanase 10A, as a mock protein of interest.

   Depositor

   Roberto Chica

   Article

   Eason et al ACS Synth Biol. 2020 Nov 20;9(11):2955-2

   Insert

   TAX-L10-STEPtag
   

   Use

   Tags

   6x His tag

   Expression

   Bacterial

   Mutation

   Promoter

   T7

   Available since

   Sept. 18, 2020

   Availability

   Academic Institutions and Nonprofits only

   

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9. pET11a-STEPtag-L10-TAX

   Plasmid

   #159374

   Purpose

   Expresses STEPtag fused to the N-terminus of inactive T. aurantiacus xylanase 10A, as a mock protein of interest.

   Depositor

   Roberto Chica

   Article

   Eason et al ACS Synth Biol. 2020 Nov 20;9(11):2955-2

   Insert

   STEPtag-L10-TAX
   

   Use

   Tags

   6x His tag

   Expression

   Bacterial

   Mutation

   Promoter

   T7

   Available since

   Sept. 18, 2020

   Availability

   Academic Institutions and Nonprofits only

   

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10. pET11d-FHis-DND1-Y102A

    Plasmid

    #70082

    Purpose

    Bacterial expression of FLAG-and 6xHis-tagged human DND1 protein with defective RRM (Y102A). Codons are optimized for bacteria expression.

    Depositor

    Thomas Tuschl

    Article

    Yamaji et al Nature. 2017 Mar 23;543(7646):568-572. d

    Insert

    Dnd1 (DND1 Human)
    

    Use

    Tags

    6xHIS and FLAG

    Expression

    Bacterial

    Mutation

    Y102A. Codon optimized for bacterial expression

    Promoter

    Available since

    Nov. 22, 2017

    Availability

    Academic Institutions and Nonprofits only

    

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11. pET15b-ACP (from S. aureus)

    Plasmid

    #63686

    Purpose

    Inducible expression of Acyl Carrier Protein (ACP) from S. aureus

    Depositor

    Michael Johnson

    Insert

    ACP
    

    Use

    Tags

    His

    Expression

    Bacterial

    Mutation

    codon optimized

    Promoter

    T7

    Available since

    April 10, 2015

    Availability

    Academic Institutions and Nonprofits only

12. pET45b-tsr(B/X) (6X-His-Dm-Cofilin)

    Plasmid

    #128278

    Purpose

    Expresses Drosophila melanogaster Cofilin (Twinstar) in bacterial cell culture

    Depositor

    Anna Sokac

    Article

    Figard et al Cell Rep. 2019 Mar 26;26(13):3493-3501.e

    Insert

    twinstar (tsr) (tsr Fly)
    

    Use

    Tags

    6X Histidine tag

    Expression

    Bacterial

    Mutation

    Met1 changed to Pro1; Cys77 silent basepair mutat…

    Promoter

    T7

    Available since

    Nov. 22, 2019

    Availability

    Academic Institutions and Nonprofits only

    

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13. pDRF1-GW ymYPET-T2A-mTurquoise2

    Plasmid

    #118444

    Purpose

    Produces equimolar expression of ymYPET and mTq2

    Depositor

    Bas Teusink

    Article

    Botman et al Sci Rep. 2019 Feb 19;9(1):2234. doi: 10.

    Insert

    ymYPET-T2A-mTurquoise2
    

    Use

    Tags

    Expression

    Yeast

    Mutation

    YPET A206K, F208S, E232L, N235D

    Promoter

    Available since

    Feb. 26, 2019

    Availability

    Academic Institutions and Nonprofits only

    

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14. pET-DEST42-ARL6-V5-His6

    Plasmid

    #67422

    Purpose

    Expression of hArl6 with C-term V5 and His6 tag in E. coli

    Depositor

    Richard Kahn

    Article

    Kerr et al Cell Logist. 2015 Sep 21;5(3):e1090523.

    Insert

    ARL6 (ARL6 Human)
    

    Use

    Tags

    His and V5

    Expression

    Bacterial

    Mutation

    Promoter

    T7/lacO

    Available since

    Oct. 5, 2015

    Availability

    Academic Institutions and Nonprofits only

15. HOXC13(human) GSTfusion-pET

    Plasmid

    #8680

    Depositor

    Corey Largman

    Article

    Komuves et al Dev Dyn 2003 Jun;227(2):192-202.

    Insert

    HOXC13 (HOXC13 Human)
    

    Use

    Tags

    GST

    Expression

    Bacterial

    Mutation

    makes protein by Coomassie blue staining

    Promoter

    Available since

    May 30, 2006

    Availability

    Academic Institutions and Nonprofits only

16. M26I DJ-1 TEV site pET15b

    Plasmid

    #60685

    Purpose

    Recombinant protein expression of Park7

    Depositor

    Mark Wilson

    Article

    Milkovic et al Protein Sci. 2015 Oct;24(10):1671-85. do

    Insert

    M26I DJ-1 (PARK7 Human)
    

    Use

    Tags

    6xHis

    Expression

    Bacterial

    Mutation

    M26I and TEV site was inserted (see comments belo…

    Promoter

    T7

    Available since

    Oct. 24, 2014

    Availability

    Academic Institutions and Nonprofits only

17. pETNKI-his-3C-LIC-Amp

    Plasmid

    #108704

    Purpose

    Expression of a His-tagged target protein with 3C protease cleavage site.

    Depositor

    Titia Sixma

    Article

    Luna-Vargas et al J Struct Biol. 2011 Aug;175(2):113-9. do

    Type

    Empty backbone

    Use

    Tags

    His-3C protease cleavage site

    Expression

    Bacterial

    Mutation

    Promoter

    T7

    Available since

    Aug. 1, 2018

    Availability

    Academic Institutions and Nonprofits only

    

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18. pETM6-Pc4CL-PhCHS-CmCHI

    Plasmid

    #73397

    Purpose

    Expresses three enzymes to convert phenylpropanoic acids to flavanones in vivo

    Depositor

    Mattheos Koffas

    Article

    Jones et al Metab Eng. 2016 Feb 6;35:55-63. doi: 10.

    Inserts

    Pc4CL
    PhCHS
    CmCHI
    

    Use

    Tags

    Expression

    Bacterial

    Mutation

    Promoter

    T7

    Available since

    March 1, 2016

    Availability

    Academic Institutions and Nonprofits only

    

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19. pET28b-NmCas9-Flag H588A

    Plasmid

    #109032

    Purpose

    H588A mutant of Cas9 from Nm8013 cloned into pET28b with a C-terminal Flag tag for protein expression

    Depositor

    Yan Zhang

    Article

    Rousseau et al Mol Cell. 2018 Mar 1;69(5):906-914.e4. d

    Insert

    CRISPR-associated endonuclease Cas9
    

    Use

    Tags

    Flag Tag

    Expression

    Bacterial

    Mutation

    Promoter

    T7

    Available since

    May 11, 2018

    Availability

    Academic Institutions and Nonprofits only

    

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20. pET15b+PCNA3 R112C/T180C

    Plasmid

    #66182

    Purpose

    Expresses the R112C/T180C mutant of Sulfolobus solfataricus PCNA3

    Depositor

    Teruyuki Nagamune

    Article

    Hirakawa et al Biotechnol Bioeng. 2013 Jul;110(7):1858-

    Insert

    DNA polymerase sliding clamp 3
    

    Use

    Tags

    His6 tag

    Expression

    Bacterial

    Mutation

    R112C, T180C

    Promoter

    T7 promoter

    Available since

    June 30, 2015

    Availability

    Academic Institutions and Nonprofits only

    

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