We narrowed to 18,420 results for: REV

1. pRG645_lexO-AscI_LexA-TF_LEU2MX

   Plasmid

   #154813

   Purpose

   Inducible expression of AscI endonuclease in S. cerevisiae

   Depositor

   Lorraine Symington

   Article

   Gnugge et al Nucleic Acids Res. 2020 Oct 14. pii: 592

   Inserts

   AscI endonuclease
   LexA-ER-B112
   

   Tags

   FLAG and NLS

   Expression

   Yeast

   Promoter

   ACT1 and lexO_4-CYC1_core

   Available Since

   Oct. 26, 2020

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

2. pET151-Vps4

   Plasmid

   #87733

   Purpose

   Bacterial expression of full-length S. cerevisiae Vps4

   Depositor

   Chris Hill

   Article

   Monroe et al Elife. 2017 Apr 5;6. pii: e24487. doi: 1

   Insert

   Vacuolar protein sorting-associated protein 4 (VPS4 Budding Yeast)
   

   Tags

   6xHis-V5-TEV

   Expression

   Bacterial

   Promoter

   T7

   Available Since

   April 10, 2017

   Availability

   Academic Institutions and Nonprofits only

   

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3. pDDGFP-Leu2d-ScVrg4

   Plasmid

   #102334

   Purpose

   Expresses WT ScVrg4 in S. cerevisiae cells using Galactose induction, -URA and -Leu selection

   Depositor

   Simon Newstead

   Article

   Parker et al Nature. 2017 Nov 23;551(7681):521-524. d

   Insert

   YGL225W (VRG4 Budding Yeast)
   

   Tags

   GFP

   Expression

   Yeast

   Promoter

   GAL1

   Available Since

   Oct. 17, 2017

   Availability

   Academic Institutions and Nonprofits only

   

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4. pICH47732:PtFCP:ShBle

   Plasmid

   #104893

   Purpose

   Expresses ShBle with FCP promoter/terminator, Golden Gate Assembly, for Phaeodactylum transformation.

   Depositor

   Thomas Mock

   Article

   Graff van Creveld et al Front Microbiol. 2021 Sep 8;12:688199. d

   Insert

   ShBle
   

   Use

   Phaeodactylum; golden gate assembly

   Tags

   PtFCP promoter and terminator

   Available Since

   Dec. 28, 2017

   Availability

   Academic Institutions and Nonprofits only

   

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5. pcDNA3.1-RaACR_687-EYFP

   Plasmid

   #111077

   Purpose

   Anion channelrhodopsin from Rhodomonas abbreviata for expression in mammalian cells

   Depositor

   John Spudich

   Article

   Govorunova et al eNeuro. 2018 Jul 10;5(3). pii: eN-MNT-01

   Insert

   Anion channelrhodopsin RaACR_687
   

   Tags

   EYFP

   Expression

   Mammalian

   Available Since

   June 4, 2018

   Availability

   Academic Institutions and Nonprofits only

   

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6. cPE

   Plasmid

   #183549

   Purpose

   Expresses compact Prime Editor in mammalian cells

   Depositor

   Wen Xue

   Article

   Zheng et al Mol Ther. 2022 Mar 2;30(3):1343-1351. do

   Insert

   SpCas9 nickase (H840A), Reverse Transcriptase
   

   Expression

   Mammalian

   Promoter

   CMV

   Available Since

   June 24, 2022

   Availability

   Academic Institutions and Nonprofits only

   

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7. pHES609 [prNOp7-PBAC]

   Plasmid

   #103017

   Purpose

   A S. cerevisiae plasmid. A photosensitive adenylate cyclase that produces cAMP in response to blue light. Used to activate PKA.

   Depositor

   Hana El-Samad

   Article

   Stewart-Ornstein et al Mol Biol Cell. 2017 Jan 1;28(1):221-227.

   Insert

   bPAC
   

   Expression

   Yeast

   Available Since

   March 27, 2019

   Availability

   Academic Institutions and Nonprofits only

   

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8. Let7R-SunTag

   Plasmid

   #196929

   Purpose

   Reverse complement of let7-miRNA binding sites (3x) were integrated in between the stop codon and MS2 stem-loops in the SunTag reporter.

   Depositor

   Jeffrey Chao

   Article

   Dave et al Mol Cell. 2023 Feb 16;83(4):589-606.e6.

   Insert

   Renilla luciferase
   

   Expression

   Bacterial and Mammalian

   Available Since

   March 15, 2023

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

9. pILGFPQ4

   Plasmid

   #83559

   Purpose

   Used to evaluate the expression output of GAL7 promoter with yEGFP used as the reporter gene.

   Depositor

   Claudia Vickers

   Article

   Peng et al Biotechnol Biofuels. 2017 Feb 21;10:43.

   Insert

   GAL7 promoter-yEGFP
   

   Expression

   Yeast

   Promoter

   GAL7

   Available Since

   Dec. 15, 2016

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

10. Dom34(full length)

    Plasmid

    #19098

    Depositor

    SeWon Suh

    Article

    Lee et al Mol Cell. 2007 Sep 21. 27(6):938-50.

    Insert

    Protein DOM34 (DOM34 Budding Yeast)
    

    Tags

    His

    Expression

    Bacterial

    Available Since

    June 22, 2010

    Availability

    Academic Institutions and Nonprofits only

11. iRFP713-FRB-Mss4-Kina

    Plasmid

    #202744

    Purpose

    expresses recruitable fluorescent protein-tagged enzyme

    Depositor

    Gerry Hammond

    Article

    Wills et al J Cell Sci. 2023 Aug 3:jcs.261494. doi:

    Insert

    iRFP713:MTOR(2021-2113):GGSA2:S. cerevisiae Mss4 (377-756) (MSS4 Budding Yeast)
    

    Expression

    Mammalian

    Available Since

    July 5, 2023

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

12. Let7R-IRE-TREAT

    Plasmid

    #196934

    Purpose

    Reverse complement of let7-miRNA binding sites (3x) were integrated in between the stop codon and TREAT cassette in IRE-TREAT.

    Depositor

    Jeffrey Chao

    Article

    Dave et al Mol Cell. 2023 Feb 16;83(4):589-606.e6.

    Insert

    Renilla luciferase
    

    Expression

    Bacterial and Mammalian

    Available Since

    March 15, 2023

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

13. pYES2.1-cerMET5

    Plasmid

    #107437

    Purpose

    Overexpression of MET fusion protein in S. cerevisiae

    Depositor

    Clare O'Connor

    Article

    Reeves et al CBE Life Sci Educ. 2018 Spring;17(1). pi

    Insert

    MET5 (MET5 Budding Yeast)
    

    Tags

    V5-His

    Expression

    Yeast

    Mutation

    V81I, K341E, P597L, Y605C, E741G, D932G, N1217S, …

    Promoter

    GAL1

    Available Since

    Aug. 9, 2018

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

14. pAGM4723-Del

    Plasmid

    #112207

    Purpose

    Level 2 cloning vector, for golden gate cloning for diatoms. All the Agrobacterium T-DNA delivery were deleted.

    Depositor

    Thomas Mock

    Article

    Graff van Creveld et al Elife. 2023 Jun 6;12:e84392. doi: 10.755

    Type

    Empty backbone

    Use

    Golden gate assembly

    Available Since

    July 31, 2018

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

15. JBEI-6833

    Plasmid

    #84983

    Purpose

    Expression of NudB (E. coli) and PMD (S. cerevisiae) are under control of Trc promoter for isopentenol production.

    Depositor

    Taek Soon Lee

    Article

    George et al Sci Rep. 2015 Jun 8;5:11128. doi: 10.103

    Insert

    NudB-PMD
    

    Expression

    Bacterial

    Promoter

    trc promoter

    Available Since

    Dec. 5, 2016

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

16. pRG711_lexO-AscI_LexA-TF_HIS3MX

    Plasmid

    #154820

    Purpose

    Inducible expression of AscI endonuclease in S. cerevisiae

    Depositor

    Lorraine Symington

    Article

    Gnugge et al Nucleic Acids Res. 2020 Oct 14. pii: 592

    Inserts

    AscI endonuclease
    LexA-ER-B112
    

    Tags

    FLAG and NLS

    Expression

    Yeast

    Promoter

    ACT1 and lexO_4-CYC1_core

    Available Since

    Oct. 26, 2020

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

17. pET151-Vta1(280-330)

    Plasmid

    #87738

    Purpose

    Bacterial expression of the VSL domain of S. cerevisiae Vta1

    Depositor

    Chris Hill

    Article

    Monroe et al Elife. 2017 Apr 5;6. pii: e24487. doi: 1

    Insert

    Vacuolar protein sorting-associated protein Vta1 (VTA1 Budding Yeast)
    

    Tags

    6xHis-V5-TEV

    Expression

    Bacterial

    Mutation

    deleted amino acids 1-279

    Promoter

    T7

    Available Since

    April 10, 2017

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

18. pICH47742:PtFCP:Cas9YFP

    Plasmid

    #104894

    Purpose

    Cas9, with FCP promoter/terminator, Golden Gate Assembly, for Phaeodactylum transformation

    Depositor

    Thomas Mock

    Article

    Graff van Creveld et al Front Microbiol. 2021 Sep 8;12:688199. d

    Insert

    Cas9
    

    Use

    CRISPR; ; golden gate assembly

    Tags

    YFP

    Available Since

    July 11, 2018

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

19. pILGFPQ3

    Plasmid

    #83558

    Purpose

    Used to evaluate the expression output of GAL2 promoter with yEGFP used as the reporter gene.

    Depositor

    Claudia Vickers

    Article

    Peng et al Biotechnol Biofuels. 2017 Feb 21;10:43.

    Insert

    GAL2 promoter-yEGFP
    

    Expression

    Yeast

    Promoter

    GAL2

    Available Since

    Dec. 15, 2016

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

20. Dom34+GST

    Plasmid

    #19099

    Depositor

    SeWon Suh

    Article

    Lee et al Mol Cell. 2007 Sep 21. 27(6):938-50.

    Insert

    Protein DOM34 (DOM34 Budding Yeast)
    

    Tags

    C tag, GST, and N tag

    Expression

    Bacterial

    Available Since

    Dec. 15, 2008

    Availability

    Academic Institutions and Nonprofits only