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pRNA-U6-Zip let-7

Plasmid

#35670

Depositor

Phillip Zamore

Article

Xie et al Nat Methods. 2012 Mar 4;9(4):403-9. doi:

Insert

let-7Zip

Use

Tags

Expression

Mutation

Promoter

U6

Available since

July 11, 2012

Availability

Academic Institutions and Nonprofits only

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pAAV-FLEX-SaCas9-U6-sgGabrg3

Plasmid

#124858

Purpose

Mutagenesis of Gabrg3

Depositor

Larry Zweifel

Article

Hunker et al Cell Rep. 2020 Mar 24;30(12):4303-4316.e

Insert

Gabrg3 (Gabrg3 Mouse)

Use

AAV, CRISPR, and Mouse Targeting

Tags

Expression

Mutation

Promoter

Available since

May 6, 2019

Availability

Academic Institutions and Nonprofits only

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pAAV-FLEXfrt-SaCas9-U6-sgGrin2a

Plasmid

#124863

Purpose

Mutagenesis of Grin2a

Depositor

Larry Zweifel

Article

Hunker et al Cell Rep. 2020 Mar 24;30(12):4303-4316.e

Insert

Grin2a (Grin2a Mouse)

Use

AAV, CRISPR, and Mouse Targeting

Tags

Expression

Mutation

Promoter

Available since

May 6, 2019

Availability

Academic Institutions and Nonprofits only

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pAAV-FLEXfrt-SaCas9-U6-sgSlc17a7

Plasmid

#124859

Purpose

Mutagenesis of Slc17a7

Depositor

Larry Zweifel

Article

Hunker et al Cell Rep. 2020 Mar 24;30(12):4303-4316.e

Insert

Slc17a7 (Slc17a7 Mouse)

Use

AAV, CRISPR, and Mouse Targeting

Tags

Expression

Mutation

Promoter

Available since

May 6, 2019

Availability

Academic Institutions and Nonprofits only

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pAAV-FLEXfrt-SaCas9-U6-sgGria3

Plasmid

#124869

Purpose

Mutagenesis of Gria3

Depositor

Larry Zweifel

Article

Hunker et al Cell Rep. 2020 Mar 24;30(12):4303-4316.e

Insert

Gria3 (Gria3 Mouse)

Use

AAV, CRISPR, and Mouse Targeting

Tags

Expression

Mutation

Promoter

Available since

May 6, 2019

Availability

Academic Institutions and Nonprofits only

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pAAV-FLEXfrt-SaCas9-U6-sgGabrg3

Plasmid

#124872

Purpose

Mutagenesis of Gabrg3

Depositor

Larry Zweifel

Article

Hunker et al Cell Rep. 2020 Mar 24;30(12):4303-4316.e

Insert

Gabrg3 (Gabrg3 Mouse)

Use

AAV, CRISPR, and Mouse Targeting

Tags

Expression

Mutation

Promoter

Available since

May 6, 2019

Availability

Academic Institutions and Nonprofits only

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pAAV-FLEXfrt-SaCas9-U6-sgGabrg2

Plasmid

#124871

Purpose

Mutagenesis of Gabrg2

Depositor

Larry Zweifel

Article

Hunker et al Cell Rep. 2020 Mar 24;30(12):4303-4316.e

Insert

Gabrg2 (Gabrg2 Mouse)

Use

AAV, CRISPR, and Mouse Targeting

Tags

Expression

Mutation

Promoter

Available since

May 3, 2019

Availability

Academic Institutions and Nonprofits only

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pCRII-Topo U6-SpAi9L-U6SpDMDL

Plasmid

#78608

Purpose

Expresses gRNAs (for SpCas9) targeting 5’ of Ai9 stop cassette and Dmd intron 22

Depositor

Amy Wagers

Article

Tabebordbar et al Science. 2016 Jan 22;351(6271):407-11. d

Insert

U6-SpAi9L-U6SpDMDL

Use

CRISPR

Tags

Expression

Mammalian

Mutation

Promoter

U6

Available since

May 19, 2021

Availability

Academic Institutions and Nonprofits only
pMuLE ENTR U6 L1-R5

Plasmid

#62111

Purpose

MuLE (Multiple Lentiviral Expression) Entry vector containing a U6 promoter for shRNA expression. Compatible with MultiSite Gateway cloning

Depositor

Ian Frew

Article

Albers et al J Clin Invest. 2015 Mar 9. pii: 79743. d

Type

Empty backbone

Use

RNAi; Mule gateway entry vector

Tags

Expression

Mammalian

Mutation

Promoter

Available since

Jan. 30, 2015

Availability

Academic Institutions and Nonprofits only

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pMuLE ENTR U6 L1-L4

Plasmid

#62112

Purpose

MuLE (Multiple Lentiviral Expression) Entry vector containing a U6 promoter for shRNA expression. Compatible with MultiSite Gateway cloning

Depositor

Ian Frew

Article

Albers et al J Clin Invest. 2015 Mar 9. pii: 79743. d

Type

Empty backbone

Use

RNAi; Mule gateway entry vector

Tags

Expression

Mammalian

Mutation

Promoter

Available since

Feb. 26, 2015

Availability

Academic Institutions and Nonprofits only

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pLKO1-puro-U6-sgRNA-LRTM2

Plasmid

#69237

Purpose

U6 driven SpCas9 sgRNA expression for LRTM2 site

Depositor

Scot Wolfe

Article

Bolukbasi et al Nat Methods. 2015 Oct 19. doi: 10.1038/n

Insert

LRTM2 sgRNA (LRTM2 Human)

Use

CRISPR and Lentiviral

Tags

Expression

Mammalian

Mutation

Promoter

U6

Available since

Oct. 20, 2015

Availability

Academic Institutions and Nonprofits only

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pEGFP-U6-shR-E2-4

Plasmid

#19812

Depositor

Zuoshang Xu

Article

Qiu et al BMC Biotechnol. 2008 . 8():77.

Insert

shRNA

Use

RNAi

Tags

Expression

Mammalian

Mutation

Promoter

Available since

Nov. 21, 2008

Availability

Academic Institutions and Nonprofits only
pEGFP-U6-shR-E2-5

Plasmid

#19813

Depositor

Zuoshang Xu

Article

Qiu et al BMC Biotechnol. 2008 . 8():77.

Insert

shRNA

Use

RNAi

Tags

Expression

Mammalian

Mutation

Promoter

Available since

Nov. 21, 2008

Availability

Academic Institutions and Nonprofits only
pUC19-U6-BsmBI_epegRNA_entry-mpknot_(LM1140)

Plasmid

#228253

Purpose

pU6 entry plamid for cloning prime editor mpknot epegRNAs (requires BsmBI or Esp3I digest and ligation of duplexed oligos)

Depositor

Benjamin Kleinstiver

Article

Silverstein et al Nature. 2025 Apr 22. doi: 10.1038/s41586

Insert

mpknot entry plasmid backbone for epegRNA expression via human U6 promoter

Use

Tags

Expression

Mammalian

Mutation

n/a

Promoter

U6

Available since

April 23, 2025

Availability

Academic Institutions and Nonprofits only

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pX330-U6-MYC-Guide-hSpCas9

Plasmid

#228574

Purpose

Expression of human MYC gRNA; CRISPR mediated gene insertion

Depositor

Marcin Imielinski

Insert

MYC gRNA (MYC Human)

Use

CRISPR

Tags

Expression

Mammalian

Mutation

Promoter

Available since

Jan. 7, 2025

Availability

Academic Institutions and Nonprofits only

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pAAV-U6-sasgRNACMV- mCherry-WPREpA

Plasmid

#217015

Purpose

S. aureus sgRNAs backbone along with mCherry expression

Depositor

Nadav Ahituv

Article

Matharu et al Science. 2018 Dec 13. pii: science.aau06

Insert

mCherry

Use

AAV and CRISPR

Tags

Expression

Mammalian

Mutation

Promoter

CMV

Available since

Dec. 12, 2024

Availability

Academic Institutions and Nonprofits only

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pX330-U6-E690_guide-CBh-hSpCas9

Plasmid

#188545

Purpose

Plasmid encoding pCas9 and gRNA for mutagenesis or gene-correction of human ABCA3 mutation at amino acid E690

Depositor

Darrell Kotton

Article

Sun et al J Clin Invest. 2024 Jan 16;134(2):e16427

Insert

gRNA

Use

CRISPR

Tags

T2A-EGFP

Expression

Mutation

Promoter

U6

Available since

Oct. 24, 2024

Availability

Academic Institutions and Nonprofits only

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pX330-U6-W308_guide-CBh-hSpCas9

Plasmid

#188546

Purpose

Plasmid encoding pCas9 and gRNA for mutagenesis or gene-correction of human ABCA3 mutation at amino acid W308

Depositor

Darrell Kotton

Article

Sun et al J Clin Invest. 2024 Jan 16;134(2):e16427

Insert

gRNA

Use

CRISPR

Tags

T2A-EGFP

Expression

Mutation

Promoter

U6

Available since

Oct. 24, 2024

Availability

Academic Institutions and Nonprofits only

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pX330-U6-L101_guide-CBh-hSpCas9

Plasmid

#188547

Purpose

Plasmid encoding pCas9 and gRNA for mutagenesis or gene-correction of human ABCA3 mutation at amino acid L101

Depositor

Darrell Kotton

Article

Sun et al J Clin Invest. 2024 Jan 16;134(2):e16427

Insert

gRNA

Use

CRISPR

Tags

T2A-EGFP

Expression

Mutation

Promoter

U6

Available since

Oct. 24, 2024

Availability

Academic Institutions and Nonprofits only

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sgRNA scaffold-spacer-mouse U6

Plasmid

#214698

Purpose

provide "sgRNA scaffold-spacer-mouse U6" fragment

Depositor

Yin Shen

Article

Ren et al Mol Cell. 2023 Dec 21;83(24):4633-4645.e

Insert

sgRNA scaffold-spacer-mouse U6

Use

CRISPR

Tags

Expression

Mutation

Promoter

Available since

May 7, 2024

Availability

Academic Institutions and Nonprofits only

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