1,878 results

1. pSEM229 - [Pmlc-1 | mNeonGreen | cbr-tbb-2 UTR]

   Plasmid

   #159896

   Purpose

   Fluorescent co-injection marker to identify extrachromosomal arrays in C. elegans

   Depositor

   Christian Froekjaer-Jensen

   Article

   El Mouridi et al MicroPubl Biol. 2020 Sep 3;2020. doi: 10

   Insert

   Pmlc-1 | mNeonGreen | cbr-tbb-2 UTR
   

   Expression

   Worm

   Available Since

   Oct. 29, 2020

   Availability

   Academic Institutions and Nonprofits only

   

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2. pMLS791

   Plasmid

   #188889

   Purpose

   Targeting vector for insertion into 5' miniMos Arm; carries floxed Cbr-unc-119 marker, Phsp16.41::Cre and Pmyo-2::2xNLS::cyOFP

   Depositor

   Erik Jorgensen

   Article

   Schwartz et al PLoS Genet. 2021 Nov 8;17(11):e1009755.

   Insert

   floxed Cbr-unc-119 marker, Phsp16.41::Cre and Pmyo-2::2xNLS::cyOFP
   

   Expression

   Worm

   Mutation

   C. elegans codom optimization and intron addition

   Available Since

   Aug. 30, 2022

   Availability

   Academic Institutions and Nonprofits only

   

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3. pRG5271Neo

   Plasmid

   #26387

   Depositor

   Denis Dupuy

   Article

   Giordano-Santini et al Nat Methods. 2010 Sep;7(9):721-3. doi: 1

   Type

   Empty backbone

   Expression

   Bacterial and Worm

   Available Since

   Oct. 15, 2010

   Availability

   Academic Institutions and Nonprofits only

   

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4. pAP974-1

   Plasmid

   #99497

   Purpose

   TEV::linker::tagRFP::3XFlag

   Depositor

   Geraldine Seydoux

   Article

   Paix et al Methods. 2017 May 15;121-122:86-93. doi:

   Insert

   TEV::linker::tagRFP::3XFlag
   

   Expression

   Bacterial

   Available Since

   Oct. 26, 2017

   Availability

   Academic Institutions and Nonprofits only

   

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5. pSA120

   Plasmid

   #59789

   Purpose

   C. elegans heat-shock driven ZIF-1 and mCherry co-expressed in the same operon

   Depositor

   Jeremy Nance

   Article

   Armenti et al Development. 2014 Dec;141(23):4640-7. do

   Inserts

   hsp-16.41 promoter
   zif-1 coding
   gpd-2/gpd-3 intergenic sequence
   mCherry (worm)
   unc-54 3' UTR
   

   Expression

   Bacterial

   Available Since

   Oct. 29, 2014

   Availability

   Academic Institutions and Nonprofits only

   

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6. pJA257

   Plasmid

   #21510

   Depositor

   Julie Ahringer

   Article

   Zeiser et al PLoS One. 2011;6(5):e20082. Epub 2011 Ma

   Insert

   (Gly)5Ala::GFP-F64LS65T::tbb-2 3'UTR
   

   Use

   Multisite gateway

   Available Since

   Aug. 28, 2009

   Availability

   Academic Institutions and Nonprofits only

   

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7. pIN4_tsp-6_mScarlet

   Plasmid

   #182935

   Purpose

   Expresses tsp-6::mScarlet under its native promoter (amphid and phasmid ciliated neurons in C. elegans)

   Depositor

   Maureen Barr

   Article

   Nikonorova et al Curr Biol. 2022 May 9;32(9):1924-1936.e6

   Insert

   Ptsp-6-3::tsp-6::flexible linker::mScarlet (C. elegans, genomic)
   

   Tags

   flexible linker (GGGGS)x3 and mScarlet tag

   Expression

   Worm

   Promoter

   tsp-6

   Available Since

   Aug. 5, 2022

   Availability

   Academic Institutions and Nonprofits only

   

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8. pJH2523

   Plasmid

   #191358

   Purpose

   Punc-25-GCaMP3::UrSL2 ::wCherry unc-54 3' UTR C.elegans DD neurou expression of GCaMP3 RFP

   Depositor

   Mei Zhen

   Article

   Lu et al Curr Biol. 2022 Nov 7;32(21):4631-4644.e

   Insert

   GCaMP3
   

   Tags

   mCherry

   Expression

   Worm

   Promoter

   Punc-25

   Available Since

   Dec. 13, 2022

   Availability

   Academic Institutions and Nonprofits only

   

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9. pSiM4

   Plasmid

   #199383

   Purpose

   Encodes dual cistronic membrane localized jGCaMP7f and mScarlet-I optimized for C. elegans expression

   Depositor

   Hang Lu

   Insert

   jGCaMP6f::ras-2CAAX::SL2::mScarlet-I::ras-2CAAX
   

   Expression

   Bacterial

   Mutation

   no mutations, except added subcellular localizati…

   Available Since

   April 24, 2023

   Availability

   Industry, Academic Institutions, and Nonprofits

   

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10. pDR3.10

    Plasmid

    #19367

    Depositor

    Geraldine Seydoux

    Article

    Merritt et al Curr Biol. 2008 Oct 14;18(19):1476-82. d

    Insert

    msp-142 ORF+3' in pDONR201
    

    Expression

    Worm

    Available Since

    Dec. 16, 2008

    Availability

    Academic Institutions and Nonprofits only

    

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11. pLT 652

    Plasmid

    #59997

    Purpose

    Expresses a hybrid dsRNA corresponding to the Caenorhabditis elegans dhc-3/him-8 genes in bacteria; ingestion of the bacteria by C elegans produces an RNAi response & leads to more male progeny

    Depositor

    Lisa Timmons

    Article

    Timmons et al FEBS Lett. 2014 Jul 24. pii: S0014-5793(

    Inserts

    dhc-3 (dhc-3 Nematode)
    him-8 (him-8 Nematode)
    

    Expression

    Bacterial

    Mutation

    partial genomic

    Available Since

    April 28, 2015

    Availability

    Academic Institutions and Nonprofits only

    

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12. pDC20

    Plasmid

    #131366

    Purpose

    expression of MEG-3 (aa1-862) in C. elegans

    Depositor

    Geraldine Seydoux

    Article

    Smith et al Elife. 2016 Dec 3;5. pii: 21337. doi: 10

    Insert

    meg-3 (aa1-862) (meg-3 Nematode)
    

    Tags

    His

    Expression

    Bacterial

    Available Since

    April 14, 2020

    Availability

    Academic Institutions and Nonprofits only

    

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13. pJH4531

    Plasmid

    #132366

    Purpose

    Prgef-1 DAF-2 unc-54 3' UTR C.elegans pan-neural expression of DAF-2. This plasmid has replaced plasmid pJH664 from the Zhen Lab.

    Depositor

    Mei Zhen

    Article

    Hung et al Development. 2014 Apr;141(8):1767-79. do

    Insert

    DAF-2 (daf-2 Nematode)
    

    Tags

    none

    Expression

    Worm

    Promoter

    Prgef-1

    Available Since

    Sept. 22, 2020

    Availability

    Industry, Academic Institutions, and Nonprofits

    

    Enlarge

14. pJH2217

    Plasmid

    #179251

    Purpose

    Psra-11::TWK-18(gf)-UrSL-wCherry unc-54 3' UTR C.elegans neural expression of TWK-18-wCherry

    Depositor

    Mei Zhen

    Article

    Kawano et al Neuron. 2011 Nov 17;72(4):572-86. doi: 1

    Insert

    TWK-18 (twk-18 Nematode)
    

    Tags

    wCherry

    Expression

    Worm

    Mutation

    M280I

    Promoter

    Psra-11

    Available Since

    Feb. 28, 2022

    Availability

    Academic Institutions and Nonprofits only

    

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15. YW249_Pzipt17-loxP-H2BGFP-miniMOS

    Plasmid

    #196063

    Purpose

    a plasmid modified from miniMOS vector pCFJ910. the modification including insertions of SL2::H2B::GFP downstream to NeoR CDS and 2 loxP sites flanking the NeoR and GFP cassettes.

    Depositor

    Wenxing Yang

    Article

    Li et al PeerJ. 2023 May 17;11:e15433. doi: 10.77

    Inserts

    H2B::GFP
    NeoR
    Minimal Mos1
    zipt17p::zipt17::zipt17 3UTR
    

    Expression

    Worm

    Available Since

    Jan. 31, 2023

    Availability

    Academic Institutions and Nonprofits only

    

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16. pJJS010

    Plasmid

    #188333

    Purpose

    PCR template for repair templates to tag a gene with a mKate2 and mIAA7 AID degron using homology-directed repair for C. elegans.

    Depositor

    Mike Boxem

    Article

    Sepers et al G3 (Bethesda). 2022 Aug 27. pii: 6677660

    Insert

    Linker::mKate2::mIAA7
    

    Expression

    Bacterial

    Available Since

    Aug. 31, 2022

    Availability

    Academic Institutions and Nonprofits only

    

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17. pJJS004

    Plasmid

    #188327

    Purpose

    PCR template for repair templates to tag a gene with a mCherry and mIAA7 AID degron using homology-directed repair for C. elegans.

    Depositor

    Mike Boxem

    Article

    Sepers et al G3 (Bethesda). 2022 Aug 27. pii: 6677660

    Insert

    Linker::mCherry::mIAA7
    

    Expression

    Bacterial

    Available Since

    Aug. 31, 2022

    Availability

    Academic Institutions and Nonprofits only

    

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18. pCG144

    Plasmid

    #21376

    Depositor

    Geraldine Seydoux

    Article

    Merritt et al Curr Biol. 2008 Oct 14;18(19):1476-82. d

    Insert

    mCherry in pDONR201
    

    Use

    Gateway

    Available Since

    June 19, 2009

    Availability

    Academic Institutions and Nonprofits only

    

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19. pJA254

    Plasmid

    #21507

    Depositor

    Julie Ahringer

    Article

    Zeiser et al PLoS One. 2011;6(5):e20082. Epub 2011 Ma

    Insert

    pmex-5::GFP-F64LS65T::(Gly)5Ala
    

    Use

    Multisite gateway

    Available Since

    Aug. 28, 2009

    Availability

    Academic Institutions and Nonprofits only

    

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20. pCFJ1208

    Plasmid

    #44490

    Depositor

    Erik Jorgensen

    Article

    Frokjaer-Jensen et al Nat Methods. 2014 May;11(5):529-34. doi:

    Insert

    Minimal Mos1 [Peft-3 tdTomato(NLS) tbb-2 UTR, unc-119]
    

    Use

    Gateway expression clone

    Available Since

    July 30, 2013

    Availability

    Academic Institutions and Nonprofits only

    

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21. pJW1846

    Plasmid

    #154326

    Purpose

    Multi-cassette for SapTrap

    Depositor

    Jordan Ward

    Article

    Ashley et al Genetics. 2021 Mar 31;217(3). pii: 61045

    Insert

    30aa linker::GFP^SEC^AID*::3xFLAG
    

    Use

    CRISPR and Cre/Lox

    Expression

    Worm

    Mutation

    F+E mutations in sgRNA

    Available Since

    Aug. 24, 2020

    Availability

    Academic Institutions and Nonprofits only

    

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22. pMLS260

    Plasmid

    #73685

    Purpose

    Punc-17::2xNLS-FLP-D5 Expression vector

    Depositor

    Erik Jorgensen

    Article

    Schwartz et al Genetics. 2016 Feb 2. pii: genetics.115.

    Insert

    Punc-17::2xNLS-FLP-D5::let-858 3'-UTR
    

    Use

    Flp/frt

    Expression

    Worm

    Mutation

    G5D

    Promoter

    Punc-17

    Available Since

    April 20, 2018

    Availability

    Academic Institutions and Nonprofits only

    

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23. pNMSB99

    Plasmid

    #199308

    Purpose

    MosSCI insertion of myo-3p::lox2272::mTagBFP2::tbb-2 3'UTR::lox2272::ChR2-HDRC::SL2::jRGECO1a::let-858 3'UTR at ttTi5605

    Depositor

    Michael Krieg

    Article

    Porta-de-la-Riva et al Nat Methods. 2023 Apr 6. doi: 10.1038/s4

    Insert

    myo-3p::lox2272::mTagBFP2::tbb-2 3'UTR::lox2272::ChR2-HDRC::SL2::jRGECO1a::let-858 3'UTR
    

    Expression

    Worm

    Mutation

    mTagBFP2 from pJJR81, ChR2(H134R;D156C), C. elega…

    Promoter

    myo-3

    Available Since

    Aug. 17, 2023

    Availability

    Academic Institutions and Nonprofits only

    

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24. pCFJ2442 - [1-2] ENTR - Fluor - ce-tagRFP-T(PATCs(900bp), NLS, no_atg, no stop)

    Plasmid

    #159858

    Purpose

    Three-fragment Gateway compatible flourophore for expression in C. elegans

    Depositor

    Christian Froekjaer-Jensen

    Article

    Aljohani et al Nat Commun. 2020 Dec 9;11(1):6300. doi:

    Insert

    [1-2] ENTR - Fluor - ce-tagRFP-T(PATCs(900bp), NLS, no_atg, no stop)
    

    Expression

    Worm

    Mutation

    Not applicable

    Available Since

    Dec. 18, 2020

    Availability

    Academic Institutions and Nonprofits only

    

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25. pDO001

    Plasmid

    #198828

    Purpose

    Inhibition of synaptic vessel release due to disruption of synaptic vessel function by reactive oxygen species production.

    Depositor

    Paul Sternberg

    Article

    Nava et al Proc Natl Acad Sci U S A. 2023 Dec 19;12

    Insert

    15xUAS::miniSOG-103L-VAMP2-mCherry::let-858 3'UTR
    

    Expression

    Worm

    Available Since

    April 14, 2023

    Availability

    Academic Institutions and Nonprofits only

    

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26. pSEM416 - mosTI unc-119 P_UPN - gfp(nls) - tbb-2

    Plasmid

    #182359

    Purpose

    Positive control for mosTI(unc-119). unc-119 rescue and pan-neuronal nuclear GFP expression after mosTI insertion.

    Depositor

    Christian Froekjaer-Jensen

    Article

    El Mouridi et al G3 (Bethesda). 2022 Jul 28. pii: 6651068

    Insert

    Ultra-pan-neuronal positive control pUNP::gfp(NLS)
    

    Expression

    Worm

    Promoter

    Promoter Ultra Pan Neuronal

    Available Since

    May 31, 2022

    Availability

    Academic Institutions and Nonprofits only

    

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27. pSH1

    Plasmid

    #90295

    Purpose

    Expresses the fat-7 gene in C. elegans intestine

    Depositor

    Anne Brunet

    Article

    Han et al Nature. 2017 Apr 13;544(7649):185-190. d

    Insert

    fat-7 (fat-7 Nematode)
    

    Tags

    No

    Expression

    Worm

    Promoter

    ges-1

    Available Since

    June 23, 2017

    Availability

    Academic Institutions and Nonprofits only

    

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28. pAS1-odr-4P::his-24::mNeptune

    Plasmid

    #124342

    Purpose

    Multicolor Worms for Neuronal ID

    Depositor

    Andrew Leifer

    Insert

    odr-4, mNeptune (odr-4 Nematode)
    

    Expression

    Worm

    Available Since

    April 16, 2019

    Availability

    Academic Institutions and Nonprofits only

    

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29. pMS051

    Plasmid

    #137025

    Purpose

    SapTrap mScarlet-I-GLO donor

    Depositor

    Bob Goldstein, Daniel Dickinson

    Insert

    C. elegans GLO-optimized mScarlet
    

    Use

    CRISPR; Saptrap donor for cloning repair templat…

    Tags

    C. elegans GLO-optimized mScarlet

    Expression

    Worm

    Available Since

    March 9, 2020

    Availability

    Academic Institutions and Nonprofits only

    

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30. pJH4733

    Plasmid

    #178898

    Purpose

    Pmyo-3 DAF-2 unc-54 3' UTR C.elegans muscle expression of DAF-2

    Depositor

    Mei Zhen

    Article

    Hung et al Development. 2014 Apr;141(8):1767-79. do

    Insert

    DAF-2 (daf-2 Nematode)
    

    Expression

    Worm

    Promoter

    Pmyo-3

    Available Since

    Jan. 5, 2022

    Availability

    Industry, Academic Institutions, and Nonprofits

    

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