Addgene

We narrowed to 1,668 results for: GAD

Showing: 601 - 640 of 1668 results

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MSCV-Luciferase-IRES-NrasG12D

Plasmid

#60834

Purpose

Mouse NrasG12D was amplified and mutated by PCR from IMAGE clone 6475312 (Invitrogen) and cloned downstream from the IRES; subsequently Luciferase was cloned upstream of the IRES

Depositor

Scott Lowe

Article

Zuber et al Genes Dev. 2009 Apr 1;23(7):877-89. doi:

Insert

NrasG12D (Nras Mouse)

Use

Retroviral

Tags

Expression

Mutation

Promoter

Available since

Nov. 13, 2014

Availability

Academic Institutions and Nonprofits only

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DN2-Gli3 in AINGpCAGGS

Plasmid

#121969

Purpose

strong Gli3 transcriptional activator

Depositor

James Briscoe

Article

Stamataki et al Genes Dev. 2005 Mar 1;19(5):626-41. doi:

Insert

DN2Gli3 (GLI3 Human)

Use

Chick electroporation

Tags

5xmyc

Expression

Mammalian

Mutation

N terminus deletion (nt1-1401)

Promoter

Available since

April 23, 2019

Availability

Academic Institutions and Nonprofits only

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pcDNA3.1-MS2-HA-HsNot4_AA

Plasmid

#148255

Purpose

Mammalian Expression of HsNot4

Depositor

Elisa Izaurralde

Article

Keskeny et al Genes Dev. 2019 Feb 1;33(3-4):236-252. d

Insert

HsNot4 (CNOT4 Human)

Use

Tags

Expression

Mammalian

Mutation

one silent mutation compared to the sequence give…

Promoter

Available since

Nov. 11, 2020

Availability

Academic Institutions and Nonprofits only

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pMSCV-SMYD2 F184A

Plasmid

#85659

Purpose

retroviral expression of human SMYD2 F184A

Depositor

Julien Sage

Article

Reynoird et al Genes Dev. 2016 Apr 1;30(7):772-85. doi:

Insert

SMYD2 (SMYD2 Human)

Use

Retroviral

Tags

Flag

Expression

Mammalian

Mutation

F184A

Promoter

LTR

Available since

Jan. 25, 2017

Availability

Academic Institutions and Nonprofits only

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pMGS55 (GFP-ARF16-PB1-MCS-P2A-OsTIR1)

Plasmid

#129667

Purpose

A repair construct to express GFP-ARF16-PB1-MCS and OsTIR1 under the control of the CMV promoter from the human AAVS1 locus

Depositor

Michael Guertin

Article

Sathyan et al Genes Dev. 2019 Oct 1;33(19-20):1441-145

Insert

OsARF16-PB1 domain

Use

Tags

GFP

Expression

Mammalian

Mutation

Promoter

Available since

Aug. 15, 2019

Availability

Academic Institutions and Nonprofits only

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pGEX4T-1 human RNF146 WWE (99-183)

Plasmid

#132613

Purpose

expresses human RNF146 WWE (99-183) in E. coli

Depositor

Wenqing Xu

Article

Wang et al Genes Dev. 2012 Feb 1;26(3):235-40. doi:

Insert

RNF146 WWE (99-183)

Use

Tags

Expression

Bacterial

Mutation

Promoter

Available since

Oct. 17, 2019

Availability

Academic Institutions and Nonprofits only

Enlarge
GST-PRDM16 880-1038

Plasmid

#53350

Purpose

bacterial expression of mouse PRDM16 fragment

Depositor

Bruce Spiegelman, Shingo Kajimura

Article

Kajimura et al Genes Dev. 2008 May 15;22(10):1397-409.

Insert

PRDM16 (Prdm16 Mouse)

Use

Tags

GST

Expression

Bacterial

Mutation

aa 880-1038

Promoter

tac

Available since

June 2, 2014

Availability

Academic Institutions and Nonprofits only
GST-PRDM16 224-454

Plasmid

#53347

Purpose

bacterial expression of mouse PRDM16 fragment

Depositor

Bruce Spiegelman, Shingo Kajimura

Article

Kajimura et al Genes Dev. 2008 May 15;22(10):1397-409.

Insert

PRDM16 (Prdm16 Mouse)

Use

Tags

GST

Expression

Bacterial

Mutation

aa 224-454

Promoter

tac

Available since

June 2, 2014

Availability

Academic Institutions and Nonprofits only
DN1-Gli3 in AINGpCAGGS

Plasmid

#121970

Purpose

medium Gli3 transcriptional activator

Depositor

James Briscoe

Article

Stamataki et al Genes Dev. 2005 Mar 1;19(5):626-41. doi:

Insert

DN2Gli3 (GLI3 Human)

Use

Chick electroporation

Tags

5xmyc

Expression

Mammalian

Mutation

N terminus deletion (nt1-864)

Promoter

Available since

April 23, 2019

Availability

Academic Institutions and Nonprofits only

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pnEA-NpM-HsNot4-GB1His6_AG

Plasmid

#148792

Purpose

Bacterial Expression of HsNot4

Depositor

Elisa Izaurralde

Article

Keskeny et al Genes Dev. 2019 Feb 1;33(3-4):236-252. d

Insert

HsNot4 (CNOT4 Human)

Use

Tags

Expression

Bacterial

Mutation

one silent mutation compared to the sequence give…

Promoter

Available since

March 16, 2023

Availability

Academic Institutions and Nonprofits only

Enlarge
GST-PRDM16 1-223

Plasmid

#53346

Purpose

bacterial expression of mouse PRDM16 fragment

Depositor

Bruce Spiegelman, Shingo Kajimura

Article

Kajimura et al Genes Dev. 2008 May 15;22(10):1397-409.

Insert

PRDM16 (Prdm16 Mouse)

Use

Tags

GST

Expression

Bacterial

Mutation

aa 1-223

Promoter

tac

Available since

June 2, 2014

Availability

Academic Institutions and Nonprofits only
GST-PRDM16 1039-1176

Plasmid

#53351

Purpose

bacterial expression of mouse PRDM16 fragment

Depositor

Bruce Spiegelman, Shingo Kajimura

Article

Kajimura et al Genes Dev. 2008 May 15;22(10):1397-409.

Insert

PRDM16 (Prdm16 Mouse)

Use

Tags

GST

Expression

Bacterial

Mutation

aa 1039-1176

Promoter

tac

Available since

June 2, 2014

Availability

Academic Institutions and Nonprofits only
GST-PRDM16 681-880

Plasmid

#53349

Purpose

bacterial expression of mouse PRDM16 fragment

Depositor

Bruce Spiegelman, Shingo Kajimura

Article

Kajimura et al Genes Dev. 2008 May 15;22(10):1397-409.

Insert

PRDM16 (Prdm16 Mouse)

Use

Tags

GST

Expression

Bacterial

Mutation

aa 681-880

Promoter

tac

Available since

June 2, 2014

Availability

Academic Institutions and Nonprofits only
GST-PRDM16 455-680

Plasmid

#53348

Purpose

bacterial expression of mouse PRDM16 fragment

Depositor

Bruce Spiegelman, Shingo Kajimura

Article

Kajimura et al Genes Dev. 2008 May 15;22(10):1397-409.

Insert

PRDM16 (Prdm16 Mouse)

Use

Tags

GST

Expression

Bacterial

Mutation

aa 455-680

Promoter

tac

Available since

June 2, 2014

Availability

Academic Institutions and Nonprofits only
GFP-SP110C

Plasmid

#65393

Purpose

GFP-tagged BRD protein, which can be used to be expressed in mammalian cells.

Depositor

Kyle Miller

Article

Gong et al Genes Dev. 2015 Jan 15;29(2):197-211. do

Insert

SP110C

Use

Tags

EmGFP and V5

Expression

Mammalian

Mutation

none

Promoter

CMV

Available since

May 12, 2015

Availability

Academic Institutions and Nonprofits only
pT7-EGFP-C1-HsGIGYF1_AE

Plasmid

#148569

Purpose

Mammalian Expression of HsGIGYF1

Depositor

Elisa Izaurralde

Article

Peter et al Genes Dev. 2017 Jun 1;31(11):1147-1161.

Insert

HsGIGYF1 (GIGYF1 Human)

Use

Tags

Expression

Mammalian

Mutation

Promoter

Available since

Nov. 1, 2022

Availability

Academic Institutions and Nonprofits only

Enlarge
pGEX6P-1 human HUWE1 WWE (1611-1703)

Plasmid

#132614

Purpose

expresses human HUWE1 WWE (1611-1703) in E. coli

Depositor

Wenqing Xu

Article

Wang et al Genes Dev. 2012 Feb 1;26(3):235-40. doi:

Insert

HUWE1 WWE (1611-1703)

Use

Tags

Expression

Bacterial

Mutation

Promoter

Available since

Oct. 22, 2019

Availability

Academic Institutions and Nonprofits only

Enlarge
GFP-SP110A

Plasmid

#65392

Purpose

GFP-tagged BRD protein, which can be used to be expressed in mammalian cells.

Depositor

Kyle Miller

Article

Gong et al Genes Dev. 2015 Jan 15;29(2):197-211. do

Insert

SP110A

Use

Tags

EmGFP and V5

Expression

Mammalian

Mutation

none

Promoter

CMV

Available since

May 12, 2015

Availability

Academic Institutions and Nonprofits only
pEGFP-WRAP53beta-WD40domain

Plasmid

#64678

Purpose

Expresses the WD40 domain of WRAP53beta

Depositor

Marianne Farnebo

Article

Henriksson et al Genes Dev. 2014 Dec 15;28(24):2726-38. d

Insert

Wd40-encoding RNA antisense to P53 (WRAP53 Human)

Use

Tags

GFP

Expression

Mammalian

Mutation

containing only WD40 domain of WRAP53beta

Promoter

CMV

Available since

May 21, 2015

Availability

Academic Institutions and Nonprofits only
pCIneo-V5-SBP-MBP-HsNot4_275-575-T411EL415E_AG

Plasmid

#148778

Purpose

Mammalian Expression of HsNot4_275-575-T411EL415E

Depositor

Elisa Izaurralde

Article

Keskeny et al Genes Dev. 2019 Feb 1;33(3-4):236-252. d

Insert

HsNot4_275-575-T411EL415E (CNOT4 Human)

Use

Tags

Expression

Mammalian

Mutation

Promoter

Available since

March 16, 2023

Availability

Academic Institutions and Nonprofits only

Enlarge
pCIneo-V5-SBP-MBP-HsNot4-del64-104-GSSG_AG

Plasmid

#148779

Purpose

Mammalian Expression of HsNot4-del64-104

Depositor

Elisa Izaurralde

Article

Keskeny et al Genes Dev. 2019 Feb 1;33(3-4):236-252. d

Insert

HsNot4-del64-104 (CNOT4 Human)

Use

Tags

Expression

Mammalian

Mutation

Promoter

Available since

March 16, 2023

Availability

Academic Institutions and Nonprofits only

Enlarge
pCIneo-V5-SBP-MBP-HsNot4-del64-189-GSSG_AG

Plasmid

#148780

Purpose

Mammalian Expression of HsNot4-del64-189

Depositor

Elisa Izaurralde

Article

Keskeny et al Genes Dev. 2019 Feb 1;33(3-4):236-252. d

Insert

HsNot4-del64-189 (CNOT4 Human)

Use

Tags

Expression

Mammalian

Mutation

Promoter

Available since

March 16, 2023

Availability

Academic Institutions and Nonprofits only

Enlarge
pCIneo-V5-SBP-MBP-HsNot4-del109-189-GSSG_AG

Plasmid

#148781

Purpose

Mammalian Expression of HsNot4-del109-189

Depositor

Elisa Izaurralde

Article

Keskeny et al Genes Dev. 2019 Feb 1;33(3-4):236-252. d

Insert

HsNot4-del109-189 (CNOT4 Human)

Use

Tags

Expression

Mammalian

Mutation

Promoter

Available since

March 16, 2023

Availability

Academic Institutions and Nonprofits only

Enlarge
pnEA-NpM-HsNot4_275-575-del377-402-GB1His6_AG

Plasmid

#148785

Purpose

E. coli of HsNot4_275-575-del307-402

Depositor

Elisa Izaurralde

Article

Keskeny et al Genes Dev. 2019 Feb 1;33(3-4):236-252. d

Insert

HsNot4_275-575-del307-402 (CNOT4 Human)

Use

Tags

Expression

Bacterial

Mutation

Promoter

Available since

March 16, 2023

Availability

Academic Institutions and Nonprofits only

Enlarge
pnEA-NpM-HsNot4_275-575-del377-424-GB1His6_AG

Plasmid

#148786

Purpose

Bacterial Expression of HsNot4_275-575-del377-424

Depositor

Elisa Izaurralde

Article

Keskeny et al Genes Dev. 2019 Feb 1;33(3-4):236-252. d

Insert

HsNot4_275-575-del377-424 (CNOT4 Human)

Use

Tags

Expression

Bacterial

Mutation

Promoter

Available since

March 16, 2023

Availability

Academic Institutions and Nonprofits only

Enlarge
pnEA-NpM-HsNot4_275-575-del400-428-GB1His6_AG

Plasmid

#148787

Purpose

E. coli of HsNot4_275-575-del400-428

Depositor

Elisa Izaurralde

Article

Keskeny et al Genes Dev. 2019 Feb 1;33(3-4):236-252. d

Insert

HsNot4_275-575-del400-428 (CNOT4 Human)

Use

Tags

Expression

Bacterial

Mutation

Promoter

Available since

March 16, 2023

Availability

Academic Institutions and Nonprofits only

Enlarge
pCIneo-V5-SBP-MBP-HsGIGYF1_286-316_AI

Plasmid

#148942

Purpose

Mammalian Expression of HsGIGYF1_286-316

Depositor

Elisa Izaurralde

Article

Peter et al Genes Dev. 2019 Oct 1;33(19-20):1355-136

Insert

HsGIGYF1_286-316 (GIGYF1 Human)

Use

Tags

Expression

Mammalian

Mutation

Promoter

Available since

March 16, 2023

Availability

Academic Institutions and Nonprofits only

Enlarge
pcDNA3.1-MS2-HA-HsNot4_275-575-del377-402_AG

Plasmid

#148753

Purpose

Mammalian Expression of HsNot4_275-575-del377-402

Depositor

Elisa Izaurralde

Article

Keskeny et al Genes Dev. 2019 Feb 1;33(3-4):236-252. d

Insert

HsNot4_275-575-del377-402 (CNOT4 Human)

Use

Tags

Expression

Mammalian

Mutation

Promoter

Available since

March 16, 2023

Availability

Academic Institutions and Nonprofits only

Enlarge
pcDNA3.1-MS2-HA-HsNot4_275-575-del377-424_AG

Plasmid

#148754

Purpose

Mammalian Expression of HsNot4_275-575-del377-424

Depositor

Elisa Izaurralde

Article

Keskeny et al Genes Dev. 2019 Feb 1;33(3-4):236-252. d

Insert

HsNot4_275-575-del377-424 (CNOT4 Human)

Use

Tags

Expression

Mammalian

Mutation

Promoter

Available since

March 16, 2023

Availability

Academic Institutions and Nonprofits only

Enlarge
pcDNA3.1-MS2-HA-HsNot4_275-575-del400-428_AG

Plasmid

#148755

Purpose

Mammalian Expression of HsNot4_275-575-del400-428

Depositor

Elisa Izaurralde

Article

Keskeny et al Genes Dev. 2019 Feb 1;33(3-4):236-252. d

Insert

HsNot4_275-575-del400-428 (CNOT4 Human)

Use

Tags

Expression

Mammalian

Mutation

Promoter

Available since

March 16, 2023

Availability

Academic Institutions and Nonprofits only

Enlarge
pCIneo-V5-SBP-MBP-HsNot4_64-104-GSSG-275-575_AG

Plasmid

#148765

Purpose

Mammalian Expression of HsNot4_64-104-GSSG_275-575

Depositor

Elisa Izaurralde

Article

Keskeny et al Genes Dev. 2019 Feb 1;33(3-4):236-252. d

Insert

HsNot4_64-104-GSSG_275-575 (CNOT4 Human)

Use

Tags

Expression

Mammalian

Mutation

Promoter

Available since

March 16, 2023

Availability

Academic Institutions and Nonprofits only

Enlarge
pCIneo-V5-SBP-MBP-HsNot4_109-189-GSSG-275-575_AG

Plasmid

#148767

Purpose

Mammalian Expression of HsNot4_109-189-GSSG_275-575

Depositor

Elisa Izaurralde

Article

Keskeny et al Genes Dev. 2019 Feb 1;33(3-4):236-252. d

Insert

HsNot4_109-189-GSSG_275-575 (CNOT4 Human)

Use

Tags

Expression

Mammalian

Mutation

Promoter

Available since

March 16, 2023

Availability

Academic Institutions and Nonprofits only

Enlarge
pCIneo-V5-SBP-MBP-HsNot4_275-575-del377-424_AG

Plasmid

#148771

Purpose

Mammalian Expression of HsNot4_275-575-del377-424

Depositor

Elisa Izaurralde

Article

Keskeny et al Genes Dev. 2019 Feb 1;33(3-4):236-252. d

Insert

HsNot4_275-575-del377-424 (CNOT4 Human)

Use

Tags

Expression

Mammalian

Mutation

Promoter

Available since

March 16, 2023

Availability

Academic Institutions and Nonprofits only

Enlarge
pCIneo-V5-SBP-MBP-HsNot4_275-575-del400-428_AG

Plasmid

#148772

Purpose

Mammalian Expression of HsNot4_275-575-del400-428

Depositor

Elisa Izaurralde

Article

Keskeny et al Genes Dev. 2019 Feb 1;33(3-4):236-252. d

Insert

HsNot4_275-575-del400-428 (CNOT4 Human)

Use

Tags

Expression

Mammalian

Mutation

Promoter

Available since

March 16, 2023

Availability

Academic Institutions and Nonprofits only

Enlarge
pCIneo-V5-SBP-MBP-HsNot4_275-575-F405D_AG

Plasmid

#148773

Purpose

Mammalian Expression of HsNot4_275-575-F405D

Depositor

Elisa Izaurralde

Article

Keskeny et al Genes Dev. 2019 Feb 1;33(3-4):236-252. d

Insert

HsNot4_275-575-F405D (CNOT4 Human)

Use

Tags

Expression

Mammalian

Mutation

Promoter

Available since

March 16, 2023

Availability

Academic Institutions and Nonprofits only

Enlarge
pCIneo-V5-SBP-MBP-HsNot4_275-575-F408A_AG

Plasmid

#148774

Purpose

Mammalian Expression of HsNot4_275-575-F408A

Depositor

Elisa Izaurralde

Article

Keskeny et al Genes Dev. 2019 Feb 1;33(3-4):236-252. d

Insert

HsNot4_275-575-F408A (CNOT4 Human)

Use

Tags

Expression

Mammalian

Mutation

Promoter

Available since

March 16, 2023

Availability

Academic Institutions and Nonprofits only

Enlarge
pCIneo-V5-SBP-MBP-HsNot4_275-575-FFTLtoDAEE_AG

Plasmid

#148775

Purpose

Mammalian Expression of HsNot4_275-575-FFTLtoDAEE

Depositor

Elisa Izaurralde

Article

Keskeny et al Genes Dev. 2019 Feb 1;33(3-4):236-252. d

Insert

HsNot4_275-575-FFTLtoDAEE (CNOT4 Human)

Use

Tags

Expression

Mammalian

Mutation

Promoter

Available since

March 16, 2023

Availability

Academic Institutions and Nonprofits only

Enlarge
pCIneo-V5-SBP-MBP-HsNot4_275-575-L415E_AG

Plasmid

#148776

Purpose

Mammalian Expression of HsNot4_275-575-L415E

Depositor

Elisa Izaurralde

Article

Keskeny et al Genes Dev. 2019 Feb 1;33(3-4):236-252. d

Insert

HsNot4_275-575-L415E (CNOT4 Human)

Use

Tags

Expression

Mammalian

Mutation

Promoter

Available since

March 16, 2023

Availability

Academic Institutions and Nonprofits only

Enlarge
pCIneo-V5-SBP-MBP-HsNot4_275-575-T411E_AG

Plasmid

#148777

Purpose

Mammalian Expression of HsNot4_275-575-T411E

Depositor

Elisa Izaurralde

Article

Keskeny et al Genes Dev. 2019 Feb 1;33(3-4):236-252. d

Insert

HsNot4_275-575-T411E (CNOT4 Human)

Use

Tags

Expression

Mammalian

Mutation

Promoter

Available since

March 16, 2023

Availability

Academic Institutions and Nonprofits only

Enlarge
pnEA-NpM-HsGIGYF1_33-52-GB1_AE

Plasmid

#148552

Purpose

Bacterial Expression of HsGIGYF1_33-52

Depositor

Elisa Izaurralde

Article

Peter et al Genes Dev. 2017 Jun 1;31(11):1147-1161.

Insert

HsGIGYF1_33-52 (GIGYF1 Human)

Use

Tags

Expression

Bacterial

Mutation

Promoter

Available since

March 16, 2023

Availability

Academic Institutions and Nonprofits only

Enlarge

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