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VPS13D^mScarlet
Plasmid#174110PurposeThe insert is a codon optimized DNA sequence encoding VPS13D with an internal mScarlet protein.DepositorInsertCodon optimized VPS13D
UseTagsmScarletExpressionMammalianMutationWith an internal mScarlet tag in aminoacid positi…PromoterCMVAvailable sinceDec. 3, 2021AvailabilityAcademic Institutions and Nonprofits only -
pPW3756 : CMVd3-ERmembrane-HaloTag-KKMP
Plasmid#185678PurposeLow-level transient expression of a single HaloTag protein with a C-terminal KKMP ER retention signal, targeted to the ER membrane.DepositorInsertERmembrane-HaloTag-KKMP
UseTagsExpressionMammalianMutationPromoterAvailable sinceMarch 14, 2023AvailabilityAcademic Institutions and Nonprofits only -
Rtn2b-mCherry
Plasmid#186600PurposeEncodes human full-length Reticulon 2 isoform B fused to mCherryDepositorInsertHuman Reticulon 2 isoform B (RTN2 Human)
UseTagsmCherryExpressionMammalianMutationPromoterCMVAvailable sinceFeb. 1, 2023AvailabilityAcademic Institutions and Nonprofits only -
pEM705 Gst (Ser)
Plasmid#109354PurposeGsX chimera with Gαt C-terminus (Pertussis toxin insensitive mutant)DepositorInsertGst chimera
UseTagsNoneExpressionMammalianMutationChanged Cysteine at position 391 to Serine (Pertu…PromoterCAGAvailable sinceMay 11, 2018AvailabilityAcademic Institutions and Nonprofits only -
Rtn2b-mNeon
Plasmid#186598PurposeEncodes human full-length Reticulon 2 isoform B fused to mNeonGreenDepositorInsertHuman Reticulon 2 isoform B (RTN2 Human)
UseTagsmNeonGreenExpressionMammalianMutationPromoterCMVAvailable sinceFeb. 1, 2023AvailabilityAcademic Institutions and Nonprofits only -
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mNeonGreen-Sec31A
Plasmid#226582PurposeEncodes Sec31A protein labeled with mNeonGreenDepositorInsertSec31A (Protein transport protein) (SEC31A Human)
UseTagsmNeonGreenExpressionMammalianMutationPromoterAvailable sinceNov. 12, 2024AvailabilityAcademic Institutions and Nonprofits only -
pLV-dfROX
Plasmid#137170PurposeLentiviral transfer vector for expression of roGFP2 and HyPer RedDepositorInsertroGFP2-2A-HyPer Red
UseLentiviralTagsExpressionMutationPromoterEF1aAvailable sinceApril 30, 2021AvailabilityAcademic Institutions and Nonprofits only -
pF(UG) hSyn SYP-HaloTag
Plasmid#188907PurposeExpresses synaptic HaloTagDepositorInsertSYP (Syp Mouse)
UseLentiviralTagsHaloTagExpressionMutationPromoterAvailable sinceOct. 17, 2023AvailabilityAcademic Institutions and Nonprofits only -
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pCAG-AKAP-mtagBFP2-UBC6
Plasmid#105007PurposeMammalian Expression of mtagBFP2DepositorInsertmtagBFP2
UseTagsExpressionMammalianMutationPromoterCAGAvailable sinceJan. 17, 2018AvailabilityAcademic Institutions and Nonprofits only -
pX330GFP-hU6-gRNA-hSpCas9
Plasmid#128385PurposeGFP positive gRNA/hSpCas9 constructDepositorInsertGFP, gRNA and hSpCas9
UseCRISPRTagsExpressionMutationPromoterAvailable sinceSept. 20, 2019AvailabilityAcademic Institutions and Nonprofits only -
pQCXIP GFP-TREX1
Plasmid#164245Purposeretroviral plasmid for GFP-TREX1DepositorInsertTREX1 (TREX1 Human)
UseRetroviralTagsGFPExpressionMammalianMutationPromoterCMVAvailable sinceApril 13, 2021AvailabilityAcademic Institutions and Nonprofits only -
p3XFLAG-CMV 7.1_syn6
Plasmid#50012PurposeEncodes syntaxin 6 with a N-terminal 3X FLAG tagDepositorInsertsyntaxin 6 (Stx6 Rat)
UseTags3x FLAGExpressionMammalianMutationPromoterCMVAvailable sinceAug. 5, 2017AvailabilityAcademic Institutions and Nonprofits only -
mito-mScarlet
Plasmid#141153PurposeFluorescent labeling of the mitochondrial matrix in mammalian cellsDepositorInsertCOX4 (COX4 Budding Yeast)
UseTagsmScarletExpressionMammalianMutationPromoterCMVAvailable sinceAug. 28, 2020AvailabilityAcademic Institutions and Nonprofits only -
pQCXIP-GFP-RPA70
Plasmid#164231Purposeretroviral plasmid for GFP-RPA70DepositorInsertRPA70 (RPA1 Human)
UseRetroviralTagsGFPExpressionMammalianMutationPromoterCMVAvailable sinceMarch 15, 2021AvailabilityAcademic Institutions and Nonprofits only -
pCMV-FLAG-PGRMC2
Plasmid#173468PurposeHuman PGRMC2 containing an N-terminal FLAG tagDepositorInsertPGRMC2 (PGRMC2 Human)
UseTagsFLAGExpressionMammalianMutationPromoterAvailable sinceAug. 20, 2021AvailabilityAcademic Institutions and Nonprofits only -
pFSynW Syt1 I367T IRES mRuby3
Plasmid#170642PurposeExpression of syt1 in cultured neurons by lentiviral transductionDepositorInsertSYT1 (Syt1 Rat)
UseLentiviralTagsExpressionMutationI367TPromoterAvailable sinceJune 18, 2024AvailabilityAcademic Institutions and Nonprofits only -
pEF Synaptophysin-GCamp6s
Plasmid#188981PurposePresynaptic fluorescent Ca2+ indicatorDepositorInsertSynaptophysin (Syp Rat, Synthetic)
UseTagsExpressionMammalianMutationPromoterAvailable sinceDec. 6, 2022AvailabilityAcademic Institutions and Nonprofits only -
pcDNA3 Rod Opsin
Plasmid#109361PurposeHuman rod opsinDepositorInsertRod opsin (RHO Human)
UseTagsExpressionMammalianMutationPromoterCMVAvailable sinceMay 9, 2018AvailabilityAcademic Institutions and Nonprofits only -
pEF HaloTag-SYB2
Plasmid#197282PurposeEncodes full-length Synaptobrevin 2 (SYB2) with mutations Q76V and F77W to confer resistance to tetanus cleavage.DepositorInsertSYB2 and HaloTag (Vamp2 Rat)
UseTagsExpressionMammalianMutationQ76V and F77WPromoterEF1alphaAvailable sinceJuly 28, 2023AvailabilityAcademic Institutions and Nonprofits only -
pGEX Syt1 C2AB (96-421)
Plasmid#170643PurposeExpression of syt1 for purification in E. ColiDepositorInsertSYT1 (Syt1 Rat)
UseTagsExpressionBacterialMutationPromoterAvailable sinceNov. 8, 2022AvailabilityAcademic Institutions and Nonprofits only -
pMT-Diaphanous-GFP
Plasmid#24287DepositorInsertDiaphanous (dia Fly)
UseTagsGFPExpressionInsectMutationPromoterAvailable sinceMay 10, 2010AvailabilityAcademic Institutions and Nonprofits only -
pFSynW Syt1 WT IRES mRuby3
Plasmid#170639PurposeExpression of syt1 in cultured neurons by lentiviral transductionDepositorInsertSYT1 (Syt1 Rat)
UseLentiviralTagsExpressionMutationPromoterAvailable sinceNov. 1, 2024AvailabilityAcademic Institutions and Nonprofits only -
pFSynW Syt1 D303G IRES mRuby3
Plasmid#170640PurposeExpression of syt1 in cultured neurons by lentiviral transductionDepositorInsertSYT1 (Syt1 Rat)
UseLentiviralTagsExpressionMutationD303GPromoterAvailable sinceNov. 1, 2024AvailabilityAcademic Institutions and Nonprofits only -
pFSynW Syt1 D365E IRES mRuby3
Plasmid#170641PurposeExpression of syt1 in cultured neurons by lentiviral transductionDepositorInsertSYT1 (Syt1 Rat)
UseLentiviralTagsExpressionMutationD365EPromoterAvailable sinceNov. 1, 2024AvailabilityAcademic Institutions and Nonprofits only -
pGEX Syt1 C2AB (96-421) D303G
Plasmid#170646PurposeExpression of syt1 for purification in E. ColiDepositorInsertSYT1 (Syt1 Rat)
UseTagsExpressionBacterialMutationD303GPromoterAvailable sinceNov. 1, 2024AvailabilityAcademic Institutions and Nonprofits only -
pGEX Syt1 C2AB (96-421) D365E
Plasmid#170649PurposeExpression of syt1 for purification in E. ColiDepositorInsertSYT1 (Syt1 Rat)
UseTagsExpressionBacterialMutationD365EPromoterAvailable sinceNov. 1, 2024AvailabilityAcademic Institutions and Nonprofits only -
pGEX Syt1 C2AB (96-421) I367T
Plasmid#170652PurposeExpression of syt1 for purification in E. ColiDepositorInsertSYT1 (Syt1 Rat)
UseTagsExpressionBacterialMutationI367TPromoterAvailable sinceNov. 1, 2024AvailabilityAcademic Institutions and Nonprofits only -
SiRES COR1C-Halo
Plasmid#195153Purposeimage COR1CDepositorInsertCORO1C (CORO1C Human)
UseTagsExpressionMammalianMutationPromoterAvailable sinceSept. 25, 2023AvailabilityAcademic Institutions and Nonprofits only -
pMRX-INU-TEX264-FLAG
Plasmid#128258PurposeExpresses TEX264 in mammalian cellsDepositorInsertTEX264 (TEX264 Human)
UseRetroviralTagsFLAGExpressionMammalianMutationPromoterCMVAvailable sinceJuly 26, 2019AvailabilityAcademic Institutions and Nonprofits only -
Cenp-B INCENP GFP
Plasmid#45238PurposeTarget INCENP to centromeres via full length CENP-BDepositorInsertCenp-B, INCENP-Δcen, GFP
UseTagsGFP and full length CENP-BExpressionMammalianMutationCEN box deleted from INCENP (aa1-46)PromoterAvailable sinceSept. 4, 2014AvailabilityAcademic Institutions and Nonprofits only -
pCAG-HASAP
Plasmid#138325Purposechemigenetic voltage indicatorDepositorInsertHASAP
UseTagsExpressionMammalianMutationPromoterCAGAvailable sinceMarch 10, 2020AvailabilityAcademic Institutions and Nonprofits only -
EcMBP165-cpGFP.PPYF.T203V.pRSET
Plasmid#33373Purposea single-wavelength maltose sensor constructed from E. coli MalE and cpGFPDepositorInsertImproved MBP based Maltose Biosensor
UseTags6xHISExpressionBacterialMutationcpGFP146 variant from GCaMP2 inserted between AA1…PromoterT7Available sinceDec. 21, 2011AvailabilityAcademic Institutions and Nonprofits only -
FRB-mCherry-EEA1
Plasmid#222419PurposeTo express an early endosome "anchor" for rapamycin-induced heterodimerisation in mammalian cellsDepositorInsertEEA1 (EEA1 Human)
UseTagsFRB-mCherryExpressionMammalianMutationPromoterAvailable sinceJuly 16, 2024AvailabilityAcademic Institutions and Nonprofits only -
pMRX-INU-FLAG-FAM134Bs
Plasmid#128261PurposeExpresses FAM134Bs in mammalian cellsDepositorInsertFAM134Bs (RETREG1 Human)
UseRetroviralTagsFLAGExpressionMammalianMutationPromoterCMVAvailable sinceJuly 26, 2019AvailabilityAcademic Institutions and Nonprofits only -
pU6-cGAS g2 Cas9-T2A-mCherry
Plasmid#164253PurposeTranscription of cGAS guide RNA and Cas9 expression for CRISPR/Cas9 in mammalian cellsDepositorInsertsgcGAS
UseCRISPRTagsExpressionMammalianMutationPromoterU6Available sinceMarch 31, 2021AvailabilityAcademic Institutions and Nonprofits only -
pRN3P_TIR1
Plasmid#119766Purposein vitro transcription for mRNADepositorInsertTIR1
UseTagsExpressionMutationPromoterAvailable sinceMarch 11, 2020AvailabilityAcademic Institutions and Nonprofits only -
pEM705 Gso (Ser)
Plasmid#109353PurposeGsX chimera with Gαo C-terminus (Pertussis toxin insensitive mutant)DepositorInsertGso chimera
UseTagsNoneExpressionMammalianMutationChanged Cysteine at position 391 to Serine (Pertu…PromoterCAGAvailable sinceMay 11, 2018AvailabilityAcademic Institutions and Nonprofits only -
pET14-Encap
Plasmid#86405PurposeExpresses T. maritima encapsulin in E. coliDepositorInsertEncapsulin
UseTagsExpressionBacterialMutationPromoterT7Available sinceApril 26, 2017AvailabilityAcademic Institutions and Nonprofits only -
pFSW Syt1 WT IRES GFP
Plasmid#133816PurposeEncodes full-length wild type synaptotagmin 1 for viral expressionDepositorInsertSyt1 (Syt1 Rat)
UseLentiviralTagsIRES-GFPExpressionMutationPromoterhSyn (human synapsin I promoter)Available sinceMay 6, 2020AvailabilityAcademic Institutions and Nonprofits only -
LacI INCENP
Plasmid#45236PurposeTarget INCENP to lac operator sequenceDepositorInsertLacI, INCENP-Δcen, mCherry (lacI )
UseTagsLacI (lac repressor protein) and mCherryExpressionMammalianMutationCEN box deleted from INCENP (aa1-46)Promotert7Available sinceSept. 4, 2014AvailabilityAcademic Institutions and Nonprofits only -
pU6-cGAS g3 Cas9-T2A-mCherry
Plasmid#164254PurposeTranscription of cGAS guide RNA and Cas9 expression for CRISPR/Cas9 in mammalian cellsDepositorInsertsgcGAS
UseCRISPRTagsExpressionMammalianMutationPromoterU6Available sinceMarch 30, 2021AvailabilityAcademic Institutions and Nonprofits only -
pT2-Tbait-UAS-Positron-ST
Plasmid#129266PurposeUAS-driven expression of Positron in zebrafishDepositorInsertPositron-ST
UseZebrafish expressionTagsExpressionMutationD92N, E199VPromoterAvailable sinceAug. 16, 2019AvailabilityAcademic Institutions and Nonprofits only -
pRN3P_Kdm5b_MUT
Plasmid#119767Purposein vitro transcription for mRNADepositorInsertKdm5b MUT
UseTagsExpressionMutationPromoterAvailable sinceNov. 8, 2019AvailabilityAcademic Institutions and Nonprofits only -
EGFP-Miro1v4
Plasmid#174111PurposeExpresses the peroxisomal variant (splice variant 4) of Miro with an N-terminal EGFP.DepositorInsertMiro1 (var4, peroxisomal splice variant)
UseTagsEGFPExpressionMammalianMutationPromoterCMVAvailable sinceOct. 7, 2021AvailabilityAcademic Institutions and Nonprofits only -
pEM705 Gso (Cys)
Plasmid#109375PurposeGsX chimera with Gαo C-terminus (Pertussis toxin sensitive)DepositorInsertGso chimera
UseTagsNoneExpressionMammalianMutationCys at position 391 (Pertussis Toxin Sensitive)PromoterCAGAvailable sinceMay 10, 2018AvailabilityAcademic Institutions and Nonprofits only -
pEM705 Gsi (Cys)
Plasmid#109373PurposeGsX chimera with Gαi C-terminus (Pertussis toxin sensitive)DepositorInsertGsi chimera
UseTagsNoneExpressionMammalianMutationCys at position 391 (Pertussis Toxin Sensitive)PromoterCAGAvailable sinceMay 11, 2018AvailabilityAcademic Institutions and Nonprofits only -
pU6-p53 gRNA-Cas9-T2A-mCherry
Plasmid#164256PurposeTranscription of p53 guide RNA and Cas9 expression for CRISPR/Cas9 in mammalian cellsDepositorInsertsg p53
UseCRISPRTagsExpressionMammalianMutationPromoterU6Available sinceMarch 31, 2021AvailabilityAcademic Institutions and Nonprofits only
