We narrowed to 7,920 results for: AMPH

1. YFP-synDAT-S/A

   Plasmid

   #48792

   Purpose

   Expresses YFP-tagged human DAT with first 5 N-terminal serines mutated to alanine in pCIHygro

   Depositor

   Jonathan Javitch

   Article

   Fog et al Neuron. 2006 Aug 17;51(4):417-29.

   Insert

   YFP-synhDAT-S/A (SLC6A3 Human)
   

   Tags

   YFP

   Expression

   Mammalian

   Mutation

   Ser2, Ser4, Ser7, Ser12, and Ser13 mutated simult…

   Available Since

   Oct. 24, 2014

   Availability

   Academic Institutions and Nonprofits only

   

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2. YFP-synDAT-S/D

   Plasmid

   #48793

   Purpose

   Expresses YFP-tagged human DAT with first 5 N-terminal serines mutated to aspartates in pCIHygro

   Depositor

   Jonathan Javitch

   Article

   Fog et al Neuron. 2006 Aug 17;51(4):417-29.

   Insert

   YFP-synhDAT-S/D (SLC6A3 Human)
   

   Tags

   YFP

   Expression

   Mammalian

   Mutation

   Ser2, Ser4, Ser7, Ser12, and Ser13 mutated simult…

   Available Since

   Oct. 24, 2014

   Availability

   Academic Institutions and Nonprofits only

   

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3. PL-5LTR-GW-A

   Plasmid

   #138480

   Purpose

   For expression of ribozyme-flanked sgRNA after the Gateway (GW) LR cloning.

   Depositor

   Akitsu Hotta

   Article

   Gee et al Nat Commun. 2020 Mar 13;11(1):1334. doi:

   Insert

   Gateway attR1/R2 cassette with chloramphenicol resistance and ccdB genes
   

   Expression

   Mammalian

   Available Since

   March 23, 2020

   Availability

   Academic Institutions and Nonprofits only

   

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4. pTUB1-Spy.dCas9-CAT-U6-sgRNA(decoy)

   Plasmid

   #171089

   Purpose

   CRISPR interference. Construct for expressing catalytically inactive Cas9 (dCas9) from Streptococcus pyogenes in Toxoplasma gondii. Suitable for generating stable cell lines.

   Depositor

   Sebastian Lourido

   Article

   Markus et al mSphere. 2021 Oct 13:e0047421. doi: 10.1

   Inserts

   Decoy sgRNA
   dCas9
   Chloramphenicol acetyltransferase
   

   Use

   CRISPR; Toxoplasma gondii expression

   Tags

   3X FLAG and Nuclear Localization Signal

   Mutation

   D10A, H840A

   Promoter

   TUB1 (Toxoplasma gondii) and U6 (Toxoplasma gondi…

   Available Since

   Oct. 4, 2021

   Availability

   Academic Institutions and Nonprofits only

   

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5. Thy1.1-G5

   Plasmid

   #234839

   Purpose

   pMP71 backbone. Expresses Thy1.1 with N-terminal 5xGly (G5)

   Depositor

   Gabriel Victora

   Article

   Nakandakari-Higa et al Nature. 2024 Mar;627(8003):399-406. doi:

   Insert

   Thy1.1 (Thy1 Mouse)
   

   Use

   Retroviral

   Tags

   EGFP-P2A-5xGly

   Expression

   Mammalian

   Available Since

   May 27, 2025

   Availability

   Academic Institutions and Nonprofits only

   

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6. pTUB1-Sth.dCas9-P2A-CAT-T2A-TagBFP

   Plasmid

   #171090

   Purpose

   CRISPR interference. Construct for expressing catalytically inactive Cas9 (dCas9) from Streptococcus thermophilus (CRISPR1 locus) in Toxoplasma gondii. Suitable for generating stable cell lines.

   Depositor

   Sebastian Lourido

   Article

   Markus et al mSphere. 2021 Oct 13:e0047421. doi: 10.1

   Inserts

   Sth1 dCas9
   Chloramphenicol acetyltransferase
   TagBFP
   

   Use

   CRISPR; Toxoplasma gondii expression

   Tags

   3xFLAG, P2A peptide, SV40 NLS, T2A peptide, and n…

   Mutation

   D9A, H599A

   Promoter

   TUB1 (Toxoplasma gondii), transcriptionally linke…

   Available Since

   Oct. 4, 2021

   Availability

   Academic Institutions and Nonprofits only

   

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7. pBGE3.0

   Plasmid

   #165988

   Purpose

   Multi-host shuttle vector that can be transferred by conjugation and replicate in S. meliloti, E. coli, S. cerevisiae, and P. tricornutum. Contains S. meliloti pSymB origin and Nm/Kan resistance.

   Depositor

   Bogumil Karas

   Article

   Brumwell et al PLoS One. 2019 Jun 17;14(6):e0206781. do

   Inserts

   RK2/RP4 origin of transfer
   repA1B1C1
   nourseothricin N-acetyl transferase
   neomycin resistance
   

   Use

   Synthetic Biology; Bacterial and yeast cloning; c…

   Available Since

   Dec. 2, 2022

   Availability

   Academic Institutions and Nonprofits only

   

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8. pAGE2.0

   Plasmid

   #165984

   Purpose

   Multi-host shuttle vector that can be transferred by conjugation and replicate in S. meliloti, E. coli, S. cerevisiae, and P. tricornutum. Contains S. meliloti pSymA origin and Tet resistance.

   Depositor

   Bogumil Karas

   Article

   Brumwell et al PLoS One. 2019 Jun 17;14(6):e0206781. do

   Inserts

   RK2/RP4 origin of transfer
   repA2B2C2
   nourseothricin N-acetyl transferase
   tetracycline resistance
   

   Use

   Synthetic Biology; Bacterial and yeast cloning; c…

   Available Since

   Oct. 27, 2022

   Availability

   Academic Institutions and Nonprofits only

   

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9. pBGE1.0

   Plasmid

   #165986

   Purpose

   Multi-host shuttle vector that can be transferred by conjugation and replicate in S. meliloti, E. coli, S. cerevisiae, and P. tricornutum. Contains S. meliloti pSymB origin and Spec resistance.

   Depositor

   Bogumil Karas

   Article

   Brumwell et al PLoS One. 2019 Jun 17;14(6):e0206781. do

   Inserts

   RK2/RP4 origin of transfer
   repA1B1C1
   nourseothricin N-acetyl transferase
   spectinomycin/streptomycin resistance
   

   Use

   Synthetic Biology; Bacterial and yeast cloning; c…

   Available Since

   Oct. 24, 2022

   Availability

   Academic Institutions and Nonprofits only

   

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10. pAGE1.0

    Plasmid

    #165983

    Purpose

    Multi-host shuttle vector that can be transferred by conjugation and replicate in S. meliloti, E. coli, S. cerevisiae, and P. tricornutum. Contains S. meliloti pSymA origin and Spec resistance.

    Depositor

    Bogumil Karas

    Article

    Brumwell et al PLoS One. 2019 Jun 17;14(6):e0206781. do

    Inserts

    RK2/RP4 origin of transfer
    repA2B2C2
    nourseothricin N-acetyl transferase
    spectinomycin/streptomycin resistance
    

    Use

    Synthetic Biology; Bacterial and yeast cloning; c…

    Available Since

    Dec. 2, 2022

    Availability

    Academic Institutions and Nonprofits only

    

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11. pBGE2.0

    Plasmid

    #165987

    Purpose

    Multi-host shuttle vector that can be transferred by conjugation and replicate in S. meliloti, E. coli, S. cerevisiae, and P. tricornutum. Contains S. meliloti pSymB origin and Tet resistance.

    Depositor

    Bogumil Karas

    Article

    Brumwell et al PLoS One. 2019 Jun 17;14(6):e0206781. do

    Inserts

    RK2/RP4 origin of transfer
    repA1B1C1
    nourseothricin N-acetyl transferase
    tetracycline resistance
    

    Use

    Synthetic Biology; Bacterial and yeast cloning; c…

    Available Since

    Oct. 27, 2022

    Availability

    Academic Institutions and Nonprofits only

    

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12. pAGE3.0

    Plasmid

    #165985

    Purpose

    Multi-host shuttle vector that can be transferred by conjugation and replicate in S. meliloti, E. coli, S. cerevisiae, and P. tricornutum. Contains S. meliloti pSymA origin and Nm/Kan resistance.

    Depositor

    Bogumil Karas

    Article

    Brumwell et al PLoS One. 2019 Jun 17;14(6):e0206781. do

    Inserts

    RK2/RP4 origin of transfer
    repA2B2C2
    nourseothricin N-acetyl transferase
    neomycin resistance
    

    Use

    Synthetic Biology; Bacterial and yeast cloning; c…

    Available Since

    Oct. 27, 2022

    Availability

    Academic Institutions and Nonprofits only

    

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13. pMDC32B-AtTAS1c-D2-B/c-AtMIR173

    Plasmid

    #137885

    Purpose

    Plant expression vector for direct cloning of synthetic trans-acting siRNAs into Arabidopsis thaliana TAS1c precursor downstream 3'D1[+]. Contains AtMIR173 for syntasi expression in any plant species.

    Depositor

    Alberto Carbonell

    Article

    Lopez-Dolz et al Nucleic Acids Res. 2020 May 12. pii: 583

    Inserts

    AtTAS1c-D2-B/c
    2x35S-AtMIR173-Tnos
    

    Expression

    Plant

    Mutation

    A. thaliana TAS1c precursor sequence including a …

    Available Since

    May 14, 2020

    Availability

    Academic Institutions and Nonprofits only

    

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14. pPos-Ma-CmR-TAG111

    Plasmid

    #197571

    Purpose

    Positive selection plasmid for selecting ncAA-encoding Methanomethylophilus alvus Pyl-RS mutants. Expresses TAG-codon interrupted chloramphenicol resistance cassette and M. alvus Pyl-tRNA(6). p15a ori

    Depositor

    Ryan Mehl

    Article

    Avila-Crump et al ACS Chem Biol. 2022 Dec 16;17(12):3458-3

    Inserts

    Chloramphenicol resistance gene - 111TAG
    T7 polymerase -TAG
    GFPuv
    M. alvus Pyl-tRNA(6)
    

    Expression

    Bacterial

    Mutation

    111TAG and 8TAG/112TAG

    Promoter

    AraC, T7, cat promoter (constitutive), and lpp (c…

    Available Since

    April 10, 2023

    Availability

    Academic Institutions and Nonprofits only

    

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15. p3E-BIN1_tv8

    Plasmid

    #126574

    Purpose

    Multisite gateway middle entry vector containing human BIN1 transcriptional variant 8 (muscle specific) with no ATG. Parton lab clone DVA.

    Depositor

    Rob Parton

    Article

    Lo et al J Cell Biol. 2021 Dec 6;220(12). pii: 21

    Insert

    BIN1 (BIN1 Human)
    

    Use

    Gateway entry clone

    Available Since

    Nov. 4, 2021

    Availability

    Academic Institutions and Nonprofits only

    

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16. pT3-EF1A-MYC-IRES-luc

    Plasmid

    #129775

    Purpose

    Transposon based vector that expresses human MYC followed by IRES and firefly luciferase

    Depositor

    Amaia Lujambio

    Article

    Ruiz de Galarreta et al Cancer Discov. 2019 Jun 11. pii: 2159-82

    Insert

    human MYC (MYC Human)
    

    Tags

    luciferase

    Expression

    Mammalian

    Promoter

    EF1A

    Available Since

    Feb. 13, 2020

    Availability

    Academic Institutions and Nonprofits only

    

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17. pDCAF3-IS5

    Plasmid

    #65220

    Purpose

    Expresses N-demethylases for the degredation of caffeine and related methylxanthines. Can be used to measure caffeine content as described in the publication listed below.

    Depositor

    Jeffrey Barrick

    Article

    Quandt et al ACS Synth Biol. 2013 Jun 21;2(6):301-7.

    Inserts

    ndmA
    ndmB
    ndmC
    ndmD
    gst9
    chlR
    

    Use

    Synthetic Biology

    Expression

    Bacterial

    Mutation

    Changed start codon from gtg to atg and Changed s…

    Promoter

    BBa_J23100

    Available Since

    Jan. 8, 2016

    Availability

    Industry, Academic Institutions, and Nonprofits

    

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18. pX330-U6-(negative selection module)-Chimeric_BB-CBh-hSpCas9

    Plasmid

    #223321

    Purpose

    It contains a ccdB-CMr negative selection module flanked by BpiI (BbsI) sites that allows excluding clones without correct insertion of sgRNA. Human codon optimized Cas9 expressing plasmid.

    Depositor

    Jonathan Arias

    Article

    Petruskeviciute et al Stem Cell Res. 2024 Dec 12;82:103628. do

    Inserts

    SpCas9 codon optimized for human (cas9 Synthetic, Human)
    chloramphenicol resistance protein
    CcdB
    

    Use

    CRISPR and Synthetic Biology

    Expression

    Mammalian

    Promoter

    CBh

    Available Since

    Dec. 13, 2024

    Availability

    Academic Institutions and Nonprofits only

    

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19. pT3-EF1A-MYC-IRES-lucOS

    Plasmid

    #129776

    Purpose

    Transposon based vector that expresses human MYC followed by IRES and firefly luciferase and the antigens SIYRYYGL (SIY), SIINFEKL (SIN, OVA257-264), and OVA323-339

    Depositor

    Amaia Lujambio

    Article

    Ruiz de Galarreta et al Cancer Discov. 2019 Jun 11. pii: 2159-82

    Insert

    human MYC (MYC Human)
    

    Tags

    OS: SIYRYYGL (SIY), SIINFEKL (SIN, OVA257-264), a…

    Expression

    Mammalian

    Promoter

    EF1A

    Available Since

    Feb. 13, 2020

    Availability

    Academic Institutions and Nonprofits only

    

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20. pminTolR4-R2

    Plasmid

    #67711

    Purpose

    mini Tol2 backbone with att4-attR2 cassette

    Depositor

    Nathan Lawson

    Type

    Empty backbone

    Available Since

    Aug. 7, 2015

    Availability

    Academic Institutions and Nonprofits only

    

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