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Plasmid#160805PurposeExpress POLD1DepositorInsertPOLD1 (POLD1 Human)
UseRetroviralTagsExpressionMutationCodon optimized C-terminal 230 amino acidsPromoterAvailable sinceJan. 26, 2021AvailabilityAcademic Institutions and Nonprofits only -
pGL3-basic-Polr2a promoter_600bps
Plasmid#21111DepositorInsertPolr2a promoter_600bps (POLR2A Human)
UseTagsExpressionMammalianMutationPromoterAvailable sinceMay 5, 2009AvailabilityAcademic Institutions and Nonprofits only -
pIRESpuro-Flag-Pol b(K72A)
Plasmid#23258DepositorInsertDNA polymerase beta (K72A) (POLB Human)
UseTagsFlagExpressionMammalianMutationK72APromoterAvailable sinceMarch 31, 2010AvailabilityAcademic Institutions and Nonprofits only -
attB-GFP-Tet1-Poly(A)
Plasmid#65559PurposePlasmid for Bxb1-mediated recombination of the GFP-Tet1 cDNA into a MIN-tagged locusDepositorInsertTet1 (Tet1 Mouse)
UseMouse Targeting; Bxb1TagsGFPExpressionMammalianMutationPromoterAvailable sinceAug. 31, 2015AvailabilityAcademic Institutions and Nonprofits only -
pLL3.7-EF-EYFP-YAP1_WW-PolyA
Plasmid#112290PurposeFluorescently labels human YAP1-2gamma isoform (504 aa) WW mutant. WQDP (199-202) in the first WW domain was changed to AQDA, and the WLDP (258-261) of the second WW domain was changed to ALDADepositorInsertEYFP-YAP1 (YAP1 Human)
UseLentiviralTagsExpressionMutationHuman YAP1-2gamma with mutations in the WW domain…PromoterEF-1 alphaAvailable sinceMay 13, 2019AvailabilityAcademic Institutions and Nonprofits only -
LC501: pMVP (L3-L2) mCherry + polyA
Plasmid#121765PurposepMVP L3-L2 entry plasmid, contains mCherry + polyA for 3- or 4-component MultiSite Gateway Pro assembly. Allows C-term mCherry fusion to gene of interest.DepositorInsertmCherry + polyA
UseSynthetic Biology; Pmvp gateway entry plasmidTagsExpressionMutationPromoterAvailable sinceJune 27, 2019AvailabilityAcademic Institutions and Nonprofits only -
Tet-pLKO-puro shPOLE1_1
Plasmid#160810PurposeExpress Dox repressible shPOLE1DepositorInsertshPOLE1_1
UseLentiviralTagsExpressionMutationPromoterAvailable sinceJan. 4, 2021AvailabilityAcademic Institutions and Nonprofits only -
attB-GFP-Dnmt3b6-Poly(A)
Plasmid#65532PurposePlasmid for Bxb1-mediated recombination of the GFP-Dnmt3b6 cDNA into a MIN-tagged locusDepositorInsertDnmt3b (Dnmt3b Mouse)
UseMouse Targeting; Bxb1TagsGFPExpressionMammalianMutationisoform 6PromoterAvailable sinceAug. 31, 2015AvailabilityAcademic Institutions and Nonprofits only -
attB-mCh-Poly(A)‐NeoR
Plasmid#65528PurposeNeoR containing plasmid for the generation of gene disruptions via Bxb1-mediated recombination into MIN-tagged cell lines. This vector can also be used to express cDNAs.DepositorInsertattB-mCherry
UseMouse Targeting; Bxb1TagsExpressionMammalianMutationPromoterAvailable sinceAug. 20, 2015AvailabilityAcademic Institutions and Nonprofits only -
YIp211-P30-His-POL30-3'
Plasmid#99546PurposeYeast integrative vector for expression of His-tagged POL30 (PCNA); URA3 markerDepositorInsertPOL30 (POL30 Budding Yeast)
UseTags6xHisExpressionYeastMutationPromoterPOL30Available sinceSept. 14, 2017AvailabilityAcademic Institutions and Nonprofits only -
attB‐mCh‐Poly(A)-PuroR
Plasmid#65529PurposePuroR containing plasmid for the generation of gene disruptions via Bxb1-mediated recombination into MIN-tagged cell lines. This vector can also be used to express cDNAs.DepositorInsertattB-mCherry
UseMouse Targeting; Bxb1TagsExpressionMammalianMutationPromoterAvailable sinceAug. 20, 2015AvailabilityAcademic Institutions and Nonprofits only -
pLL3.7-EF-EYFP-YAP1_delta5C-PolyA
Plasmid#112289PurposeFluorescently labels human YAP1-2gamma isoform (504 aa) delta5C mutant lacking the last five residues (FLTWL) in the PDZ binding domain at the N-terminus of the proteinDepositorInsertEYFP-YAP1 (YAP1 Human)
UseLentiviralTagsExpressionMutationHuman YAP1-2gamma lacking the last 5 Amino acids …PromoterEF-1 alphaAvailable sinceMay 13, 2019AvailabilityAcademic Institutions and Nonprofits only -
pLL3.7-EF-EYFP-YAP1_Y3F-PolyA
Plasmid#112288PurposeFluorescently labels human YAP1-2gamma isoform (504 aa) Y3F mutant _ tyrosines 341, 357 and 394 corresponding to tyrosines 391, 407 and 444 in this isoformDepositorInsertEYFP-YAP1 (YAP1 Human)
UseLentiviralTagsExpressionMutation3 Tyrosines (341, 357 and 394 corresponding to ty…PromoterEF-1 alphaAvailable sinceMay 13, 2019AvailabilityAcademic Institutions and Nonprofits only -
pDEST-IRES-puro-polyA
Plasmid#43967DepositorInsertpuroR
UseGateway dest vectorTagsExpressionMutationPromoterAvailable sinceSept. 9, 2013AvailabilityAcademic Institutions and Nonprofits only -
LC402: pMVP (L3-L2) eGFP + polyA
Plasmid#121763PurposepMVP L3-L2 entry plasmid, contains eGFP + polyA for 3- or 4-component MultiSite Gateway Pro assembly. Allows C-term eGFP fusion to gene of interest.DepositorInserteGFP + polyA
UseSynthetic Biology; Pmvp gateway entry plasmidTagsExpressionMutationPromoterAvailable sinceJune 27, 2019AvailabilityAcademic Institutions and Nonprofits only -
pAWH-Rluc-let-7-Poly(A)
Plasmid#50556PurposeExpression of luciferase reporter mRNA in S2 cellDepositorInsertLuciferase-let7-Poly(A)
UseLuciferaseTagsExpressionInsectMutationPromoterActin5CAvailable sinceJan. 3, 2014AvailabilityAcademic Institutions and Nonprofits only -
pBT346.6-H3.3-K4M-bGHpolyA
Plasmid#128743PurposeInducible expression of histone H3.3K4M-FLAG/HADepositorInsertH3.3K4M (H3-3A Human)
UseTagsFLAG and HAExpressionMammalianMutationK4MPromoterCAGAvailable sinceAug. 9, 2019AvailabilityAcademic Institutions and Nonprofits only -
YIp204-P30-His-POL30
Plasmid#99550PurposeYeast integrative vector for expression of His-tagged POL30 (PCNA); TRP1 markerDepositorInsertPOL30 (POL30 Budding Yeast)
UseTags6xHisExpressionYeastMutationPromoterPOL30Available sinceSept. 14, 2017AvailabilityAcademic Institutions and Nonprofits only -
KZ701: pMVP (L3-L2) AID + polyA
Plasmid#121791PurposepMVP L3-L2 entry plasmid, contains AID + polyA for 3- or 4-component MultiSite Gateway Pro assembly. Allows fusion of minimal C-term Auxin Induced Degron (AID) domain (activated by auxin) to geneDepositorInsertAID + polyA
UseSynthetic Biology; Pmvp gateway entry plasmidTagsExpressionMutationPromoterAvailable sinceJune 27, 2019AvailabilityAcademic Institutions and Nonprofits only -
pDZ306 (GLT1 POL1 24xPP7SL)
Plasmid#35196DepositorInsertGLT1 POL1 24xPP7SL
UseTagsExpressionMutationPromoterAvailable sinceFeb. 23, 2012AvailabilityAcademic Institutions and Nonprofits only