We narrowed to 866 results for: seph

1. MiniCoopR mitfa:SPRED1

   Plasmid

   #118851

   Purpose

   Expresses human SPRED1 and zebrafish mitfa specifically in zebrafish melanocytes

   Depositor

   Leonard Zon

   Article

   Ablain et al Science. 2018 Nov 1. pii: science.aau650

   Insert

   SPRED1 (SPRED1 Human, Zebrafish)
   

   Use

   Zebrafish expression, tol7

   Tags

   Expression

   Mutation

   Promoter

   Available since

   Dec. 3, 2018

   Availability

   Academic Institutions and Nonprofits only

   

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2. pEGFP-C1-MKLP1

   Plasmid

   #70145

   Purpose

   mammalian expression of MKLP1 with an eGFP fusion

   Depositor

   Masanori Mishima

   Article

   Douglas et al Curr Biol. 2010 May 25;20(10):927-33. do

   Insert

   MKLP1 (KIF23 Human)
   

   Use

   Tags

   eGFP

   Expression

   Mammalian

   Mutation

   Promoter

   CMV

   Available since

   Nov. 4, 2015

   Availability

   Academic Institutions and Nonprofits only

   

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3. pCMV-hAR

   Plasmid

   #89078

   Purpose

   mammalian expression of human androgen receptor in pCMV5

   Depositor

   Elizabeth Wilson

   Article

   Lubahn et al Science. 1988 Apr 15;240(4850):327-30.

   Insert

   human androgen receptor (hAR) full-length (AR Human)
   

   Use

   Tags

   Expression

   Mammalian

   Mutation

   Promoter

   CMV

   Available since

   April 5, 2017

   Availability

   Academic Institutions and Nonprofits only

   

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4. MiniCoopR U6:gRNA spred1, mitfa:Cas9

   Plasmid

   #118843

   Purpose

   Targets spred1 and expresses zebrafish mitfa specifically in zebrafish melanocytes

   Depositor

   Leonard Zon

   Article

   Ablain et al Science. 2018 Nov 1. pii: science.aau650

   Insert

   spred1 gRNA (spred1 Zebrafish)
   

   Use

   CRISPR; Tol2

   Tags

   Expression

   Mutation

   Promoter

   Available since

   Nov. 28, 2018

   Availability

   Academic Institutions and Nonprofits only

   

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5. B52 (empty plasmid backbone to express 2 sgRNAs)

   Plasmid

   #100708

   Purpose

   Empty plasmid backbone to express 2 sgRNAs (use BbsI and BsmBI for cloning)

   Depositor

   Alberto Ciccia

   Article

   Billon et al Mol Cell. 2017 Sep 4. pii: S1097-2765(17

   Type

   Empty backbone

   Use

   CRISPR

   Tags

   Expression

   Mammalian

   Mutation

   Promoter

   U6 promoters

   Available since

   Sept. 6, 2017

   Availability

   Academic Institutions and Nonprofits only

   

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6. pEMI91

   Plasmid

   #74888

   Purpose

   Shuffled 9I27 polyprotein with shuffled codons to allow facile sequencing and unique RE for the 9 cassettes for versatile cloning. See Depositor Comments for more information

   Depositor

   Piotr Marszalek

   Article

   Scholl et al Biomacromolecules. 2016 Jun 8.

   Insert

   Titin I27 x 9 (TTN Human)
   

   Use

   Tags

   His-tag and Strep-tag II

   Expression

   Bacterial

   Mutation

   Promoter

   T7 promoter

   Available since

   June 13, 2016

   Availability

   Academic Institutions and Nonprofits only

   

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7. MiniCoopR U6:gRNA p53, mitfa:Cas9

   Plasmid

   #118841

   Purpose

   Targets tp53 and expresses zebrafish mitfa specifically in zebrafish melanocytes

   Depositor

   Leonard Zon

   Article

   Ablain et al Science. 2018 Nov 1. pii: science.aau650

   Insert

   tp53 gRNA (tp53 Zebrafish)
   

   Use

   CRISPR; Tol2

   Tags

   Expression

   Mutation

   Promoter

   Available since

   March 21, 2019

   Availability

   Academic Institutions and Nonprofits only

   

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8. pHDR-CDKN2A-Ex2-CMV-H2B-mOrange2

   Plasmid

   #110735

   Purpose

   Recombination template for replacing CDKN2A Exon2 with Ef1alpha-H2B-mOrange2

   Depositor

   Robert Judson-Torres

   Article

   Zeng et al Cancer Cell. 2018 Jul 9;34(1):56-68.e9.

   Insert

   H2B-mOrange2
   

   Use

   Other

   Tags

   Expression

   Mutation

   Promoter

   Available since

   July 25, 2018

   Availability

   Academic Institutions and Nonprofits only

   

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9. pGL410-BRN2p

   Plasmid

   #110733

   Purpose

   Luciferase reporter for the human BRN2 promoter

   Depositor

   Robert Judson-Torres

   Article

   Zeng et al Cancer Cell. 2018 Jul 9;34(1):56-68.e9.

   Insert

   BRN2 promoter luciferase (POU3F2 Human)
   

   Use

   Luciferase

   Tags

   Expression

   Mammalian

   Mutation

   Promoter

   Available since

   July 25, 2018

   Availability

   Academic Institutions and Nonprofits only

   

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10. B270 + SPRTN sgSTOP

    Plasmid

    #100718

    Purpose

    B270 plasmid expressing SPRTN sgSTOP (cloned in BbsI site) in combination with ATP1A1 sgSTOP

    Depositor

    Alberto Ciccia

    Article

    Billon et al Mol Cell. 2017 Sep 4. pii: S1097-2765(17

    Insert

    sgSTOP targeting SPRTN (cloned using BbsI)
    

    Use

    CRISPR

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    U6 promoters

    Available since

    Sept. 19, 2017

    Availability

    Academic Institutions and Nonprofits only

    

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11. pEGFP-C1-MKLP1 S708/710A

    Plasmid

    #70153

    Purpose

    mammalian expression of MKLP1 with an eGFP fusion and S708A/S710A mutations

    Depositor

    Masanori Mishima

    Article

    Douglas et al Curr Biol. 2010 May 25;20(10):927-33. do

    Insert

    MKLP1 (KIF23 Human)
    

    Use

    Tags

    eGFP

    Expression

    Mammalian

    Mutation

    S708A, S710A

    Promoter

    CMV

    Available since

    Nov. 4, 2015

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

12. pEGFP-C1-MKLP1 S708A

    Plasmid

    #70146

    Purpose

    mammalian expression of MKLP1 with an eGFP fusion and an S708A mutation

    Depositor

    Masanori Mishima

    Article

    Douglas et al Curr Biol. 2010 May 25;20(10):927-33. do

    Insert

    MKLP1 (KIF23 Human)
    

    Use

    Tags

    eGFP

    Expression

    Mammalian

    Mutation

    S708A

    Promoter

    CMV

    Available since

    Nov. 4, 2015

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

13. pEGFP-C1-MKLP1 S710A

    Plasmid

    #70149

    Purpose

    mammalian expression of MKLP1 with an eGFP fusion and an S710A mutation

    Depositor

    Masanori Mishima

    Article

    Douglas et al Curr Biol. 2010 May 25;20(10):927-33. do

    Insert

    MKLP1 (KIF23 Human)
    

    Use

    Tags

    eGFP

    Expression

    Mammalian

    Mutation

    S710A

    Promoter

    CMV

    Available since

    Nov. 4, 2015

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

14. pVITRO1-dV-IgE/κ

    Plasmid

    #61878

    Purpose

    Human immunoglobulin epsilon antibody expression vector (kappa light chain) without variable regions. Enables Colony-PCR screening of false-positive (PCR vector template) colonies

    Depositor

    Andrew Beavil

    Article

    Dodev et al Sci Rep. 2014 Jul 30;4:5885. doi: 10.103

    Inserts

    Human immunoglobulin Epsilon constant region
    Human immunoglobulin kappa constant region
    

    Use

    Tags

    Expression

    Mammalian

    Mutation

    Deleted Variable region

    Promoter

    mEF1 Prom and rEF1 Prom

    Available since

    March 17, 2015

    Availability

    Academic Institutions and Nonprofits only

    

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15. pVITRO1-Trastuzumab-IgA1/κ

    Plasmid

    #61888

    Purpose

    Expresses HER2/neu receptor specific humanized IgA1/κ antibody isotype (Trastuzumab-IgA1/κ)

    Depositor

    Andrew Beavil

    Article

    Dodev et al Sci Rep. 2014 Jul 30;4:5885. doi: 10.103

    Inserts

    HER2/neu receptor specific humanized Alpha 1 heavy chain expression cassette
    HER2/neu receptor specific humanized kappa light chain expression cassette
    

    Use

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    mEF1 Prom and rEF1 Prom

    Available since

    March 17, 2015

    Availability

    Academic Institutions and Nonprofits only

    

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16. pVITRO1-102.1F10-IgA1/λ

    Plasmid

    #50370

    Purpose

    Expresses grass pollen allergen Phl p 7 specific human IgA1/λ antibody isotype (102.1F10-IgA1/λ)

    Depositor

    Andrew Beavil

    Article

    Dodev et al Sci Rep. 2014 Jul 30;4:5885. doi: 10.103

    Inserts

    grass pollen allergen Phl p 7 specific human Alpha 1 heavy chain expression cassette
    grass pollen allergen Phl p 7 specific human lambda light chain expression cassette
    

    Use

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    Mouse Elongation Factor 1 Alpha and Rat Elongatio…

    Available since

    Jan. 7, 2014

    Availability

    Academic Institutions and Nonprofits only

    

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17. pVITRO1-dV-IgE/λ

    Plasmid

    #61879

    Purpose

    Human immunoglobulin epsilon antibody expression vector (lambda light chain) without variable regions. Enables Colony-PCR screening of false-positive (PCR vector template) colonies

    Depositor

    Andrew Beavil

    Article

    Dodev et al Sci Rep. 2014 Jul 30;4:5885. doi: 10.103

    Inserts

    Human immunoglobulin Epsilon constant region
    Human immunoglobulin lambda constant region
    

    Use

    Tags

    Expression

    Mammalian

    Mutation

    Deleted Variable region

    Promoter

    mEF1 Prom and rEF1 Prom

    Available since

    March 2, 2015

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

18. pHIV-INK4A-zsGreen

    Plasmid

    #110728

    Purpose

    Mammalian Expression of p16INK4A IRES zsGreen

    Depositor

    Robert Judson-Torres

    Article

    Zeng et al Cancer Cell. 2018 Jul 9;34(1):56-68.e9.

    Insert

    p16INK4A IRES zsGreen (CDKN2A Human)
    

    Use

    Lentiviral

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    Available since

    July 25, 2018

    Availability

    Academic Institutions and Nonprofits only

    

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19. pHR-TetOn-mCherry-OAZ1_FS-YFP

    Plasmid

    #232356

    Purpose

    Mammalian expression of dox-inducible polyamine sensor (polyamine levels = YFP/Cherry)

    Depositor

    Ankur Jain

    Article

    Sharma et al bioRxiv [Preprint]. 2024 Nov 17:2024.08.

    Insert

    OAZ1 derived polyamine sensing module (OAZ1 Human)
    

    Use

    Lentiviral

    Tags

    eYFP and mCherry

    Expression

    Mammalian

    Mutation

    Promoter

    TRE3G

    Available since

    March 7, 2025

    Availability

    Academic Institutions and Nonprofits only

    

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20. pVITRO1-Trastuzumab-IgG1/κ

    Plasmid

    #61883

    Purpose

    Expresses HER2/neu receptor specific humanized IgG1/κ antibody isotype (Trastuzumab-IgG1/κ)

    Depositor

    Andrew Beavil

    Article

    Dodev et al Sci Rep. 2014 Jul 30;4:5885. doi: 10.103

    Inserts

    HER2/neu receptor specific humanized Gamma 1 heavy chain expression cassette
    HER2/neu receptor specific humanized kappa light chain expression cassette
    

    Use

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    mEF1 Prom and rEF1 Prom

    Available since

    March 17, 2015

    Availability

    Academic Institutions and Nonprofits only

    

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