We narrowed to 6,927 results for: 11

1. EGFP-plk1-AKAP

   Plasmid

   #132967

   Purpose

   To study the processes involved in early mitotic events, mitotic progression, and control of re-entry after DNA damage

   Depositor

   Michael Yaffe

   Article

   Kishi et al Mol Cell Biol. 2009 Jun;29(11):3134-50.

   Insert

   polo-like kinase 1-AKAP (PLK1, AKAP9 Human)
   

   Use

   Tags

   Expression

   Mammalian

   Mutation

   C terminus of full-length Plk1 fused to the peric…

   Promoter

   Available since

   Dec. 8, 2022

   Availability

   Academic Institutions and Nonprofits only

   

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2. pAAV-Syn-CsChR-GFP

   Plasmid

   #58841

   Purpose

   AAV expression of CsChR-GFP under the Syn promoter

   Depositor

   Edward Boyden

   Article

   Klapoetke et al Nat Methods. 2014 Mar;11(3):338-46. doi:

   Insert

   CsChR-GFP
   

   Use

   AAV

   Tags

   GFP

   Expression

   Mammalian

   Mutation

   Promoter

   Syn

   Available since

   Aug. 14, 2014

   Availability

   Academic Institutions and Nonprofits only

   

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3. MSCV-HA-Nr4a1-AA

   Plasmid

   #160942

   Purpose

   Generation of retrovirus for the overexpression of Nr4a1 mutant (287C 288EAA)

   Depositor

   Matthew Steinhauser

   Article

   Zhang et al J Clin Invest. 2018 Nov 1;128(11):4898-4

   Insert

   Nr4a1 (Nr4a1 Mouse)
   

   Use

   Retroviral

   Tags

   HA tag

   Expression

   Mammalian

   Mutation

   Change Cys 287 Glu 288 into Ala Ala

   Promoter

   MSCV-LTR

   Available since

   Dec. 11, 2020

   Availability

   Academic Institutions and Nonprofits only

   

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4. Exp-Rosa-EF1a-tTA-mCh-Rs1-TetO(sh)-2

   Plasmid

   #24419

   Depositor

   Edward Hsiao

   Article

   Hsiao et al Stem Cell Res Ther. 2011 Mar 4. 2(2):11.

   Insert

   RS1 (HTR4 Human)
   

   Use

   Mouse Targeting

   Tags

   FLAG and mCherry

   Expression

   Mutation

   Human 5HT4B receptor with D100A mutation, generat…

   Promoter

   Available since

   March 14, 2011

   Availability

   Academic Institutions and Nonprofits only

   

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5. pLenti-Puro-sgMARK2

   Plasmid

   #138689

   Purpose

   Expresses a human MARK2-targeting sgRNA and Cas9

   Depositor

   Reuben Shaw

   Article

   Hollstein et al Cancer Discov. 2019 Nov;9(11):1606-1627.

   Insert

   sgMARK2 human (MARK2 Human)
   

   Use

   Lentiviral

   Tags

   Expression

   Mutation

   Promoter

   hU6

   Available since

   Feb. 26, 2020

   Availability

   Academic Institutions and Nonprofits only

   

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6. pLenti-Puro-sgMARK3

   Plasmid

   #138690

   Purpose

   Expresses a human MARK3-targeting sgRNA and Cas9

   Depositor

   Reuben Shaw

   Article

   Hollstein et al Cancer Discov. 2019 Nov;9(11):1606-1627.

   Insert

   sgMARK3 human (MARK3 Human)
   

   Use

   Lentiviral

   Tags

   Expression

   Mutation

   Promoter

   hU6

   Available since

   Feb. 26, 2020

   Availability

   Academic Institutions and Nonprofits only

   

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7. pCRI001-pGEM-LS-Bar-PgpdA-dLbCas12a-VPR-Ttrpc-LS

   Plasmid

   #140193

   Purpose

   Chromosomal integration of PgpdA-dLbCas12a-VPR-TtrpC. Contains 1kb homology arms for Aspergillus nidulans and the bar gene for glufosinate selection. Filamentous fungi integrative vector.

   Depositor

   Yit Heng Chooi

   Article

   Roux et al ACS Synth Biol. 2020 Jun 11. doi: 10.102

   Inserts

   dLbCas12a-VPR
   Bar
   Homology arm (ChrIV_A_nidulans_FGSC_A4:2736011,2737053)
   Homology arm (ChrIV_A_nidulans_FGSC_A4:2790295,2791327)
   

   Use

   CRISPR and Synthetic Biology; Fungal genome integ…

   Tags

   VPR (VP64-p65-Rta), NLS

   Expression

   Mutation

   D832A DNAse deactivated

   Promoter

   PgpdA and PtrpC

   Available since

   Sept. 11, 2020

   Availability

   Academic Institutions and Nonprofits only

   

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8. p029 End-1::EnR

   Plasmid

   #11029

   Depositor

   Ramesh Shivdasani

   Article

   Shoichet et al Proc Natl Acad Sci U S A. 2000 Apr 11. 9

   Insert

   End1::EnR (end-1, en END-1 is from C. Elegans, EnR is from Drophila)
   

   Use

   Xenopus, mammalian, avian, and zebrafish

   Tags

   Expression

   Mutation

   Dominant inhibitory GATA-binding protein End1::En…

   Promoter

   Available since

   Dec. 2, 2005

   Availability

   Academic Institutions and Nonprofits only

   

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9. psiCHECK2-E2F3 3'UTR

   Plasmid

   #29470

   Depositor

   Judy Lieberman

   Article

   Lal et al Mol Cell. 2009 Sep 11. 35(5):610-25.

   Insert

   E2F3 3'UTR (E2F3 Human)
   

   Use

   Tags

   Expression

   Mammalian

   Mutation

   Promoter

   Available since

   April 27, 2011

   Availability

   Academic Institutions and Nonprofits only

10. psiCHECK2-CHEK1 3'UTR

    Plasmid

    #29478

    Depositor

    Judy Lieberman

    Article

    Lal et al Mol Cell. 2009 Sep 11. 35(5):610-25.

    Insert

    CHEK1 3'UTR (CHEK1 Human)
    

    Use

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    Available since

    April 27, 2011

    Availability

    Academic Institutions and Nonprofits only

11. pFRT/TO/HIS/FLAG/HA-API5

    Plasmid

    #38074

    Depositor

    Markus Landthaler

    Article

    Baltz et al Mol Cell. 2012 Jun 8;46(5):674-90.

    Insert

    API5 apoptosis inhibitor 5 (API5 Human)
    

    Use

    Tags

    HIS/FLAG/HA

    Expression

    Mammalian

    Mutation

    Promoter

    Available since

    Sept. 17, 2012

    Availability

    Academic Institutions and Nonprofits only

    

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12. lentiCRISPR v2-Nr4a1sgRNA1

    Plasmid

    #160945

    Purpose

    Guide RNA 1 to generate Nr4a1 knockout by CRISPR

    Depositor

    Matthew Steinhauser

    Article

    Zhang et al J Clin Invest. 2018 Nov 1;128(11):4898-4

    Insert

    Nr4a1 gRNA 1 (Nr4a1 Mouse)
    

    Use

    CRISPR and Lentiviral

    Tags

    Expression

    Mutation

    Promoter

    hU6

    Available since

    Dec. 10, 2020

    Availability

    Academic Institutions and Nonprofits only

    

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13. pEGFP-RNF138-delta 18-58

    Plasmid

    #78921

    Purpose

    Mammalian expression of RNF138 with delta 18-58 and an EGFP fusion

    Depositor

    Michael Hendzel

    Article

    Ismail et al Nat Cell Biol. 2015 Nov;17(11):1446-57.

    Insert

    RNF138 (RNF138 Human)
    

    Use

    Tags

    EGFP

    Expression

    Mammalian

    Mutation

    delta 18-58

    Promoter

    CMV

    Available since

    June 27, 2016

    Availability

    Academic Institutions and Nonprofits only

14. pUC19-U6-MbCas12a crRNA-BsmBI cassette (BPK4449)

    Plasmid

    #114088

    Purpose

    U6 promoter crRNA entry vector used for all MbCas12a crRNAs (clone spacer oligos into BsmBI cassette)

    Depositor

    Benjamin Kleinstiver, Keith Joung

    Article

    Kleinstiver et al Nat Biotechnol. 2019 Feb 11. pii: 10.103

    Insert

    entry vector for MbCas12a crRNAs
    

    Use

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    Available since

    Dec. 10, 2018

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

15. p3xFLAG-CMV-Tlr4(Lps)

    Plasmid

    #27149

    Depositor

    Bruce Beutler

    Article

    Poltorak et al Science. 1998 Dec 11. 282(5396):2085-8.

    Insert

    toll-like receptor 4(Lps) (Tlr4 Mouse)
    

    Use

    Tags

    3x FLAG

    Expression

    Mammalian

    Mutation

    changed Proline 712 to Histidine. Native signal p…

    Promoter

    Available since

    Feb. 10, 2011

    Availability

    Academic Institutions and Nonprofits only

16. pCAG-hMbCas12a-NLS(nucleoplasmin)-3xHA (AAS2134)

    Plasmid

    #114090

    Purpose

    CAG promoter expression plasmid for human codon optimized MbCas12a nuclease with C-terminal NLS and HA tag

    Depositor

    Benjamin Kleinstiver, Keith Joung

    Article

    Kleinstiver et al Nat Biotechnol. 2019 Feb 11. pii: 10.103

    Insert

    human codon optimized MbCas12a
    

    Use

    Tags

    NLS(nucleoplasmin) and 3xHA

    Expression

    Mammalian

    Mutation

    Promoter

    Available since

    Dec. 10, 2018

    Availability

    Academic Institutions and Nonprofits only

    

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17. psiCHECK2-E2F2 3'UTR

    Plasmid

    #29469

    Depositor

    Judy Lieberman

    Article

    Lal et al Mol Cell. 2009 Sep 11. 35(5):610-25.

    Insert

    E2F2 3'UTR (E2F2 Human)
    

    Use

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    Available since

    April 27, 2011

    Availability

    Academic Institutions and Nonprofits only

18. pEGFP-N3/hArf5(WT)-EGFP

    Plasmid

    #79534

    Purpose

    Expresses C-terminally EGFP-tagged Arf5(WT) in mammalian cells

    Depositor

    Kazuhisa Nakayama

    Article

    Makyio et al EMBO J. 2012 May 30;31(11):2590-603. doi

    Insert

    Arf5 (ARF5 Human)
    

    Use

    Tags

    EGFP

    Expression

    Mammalian

    Mutation

    Promoter

    Available since

    April 11, 2017

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

19. pMSCVpuro-F-Gcn5-NLSm

    Plasmid

    #63708

    Purpose

    retroviral expression of cytoplasm-localized mouse Gcn5 in mammalian cells

    Depositor

    Kai Ge

    Article

    Jin et al EMBO Rep. 2014 Nov;15(11):1192-201. doi:

    Insert

    Gcn5 (Kat2a Mouse)
    

    Use

    Retroviral

    Tags

    FLAG

    Expression

    Mammalian

    Mutation

    mutated K425, K427 and K438 to R (arginine)

    Promoter

    Available since

    April 8, 2015

    Availability

    Academic Institutions and Nonprofits only

20. pNIA-CEN-mVenus-FLAG-LANS1-Set2

    Plasmid

    #122003

    Purpose

    mVenus-FLAG-LANS-Set2: mVenus- and FLAG-tagged LANS1-Set2 for light control of Set2 localization in yeast

    Depositor

    Brian Kuhlman

    Article

    Lerner et al Genome Res. 2020 Nov;30(11):1605-1617. d

    Insert

    mVenus-FLAG-LANS1-Set2 (SET2 Budding Yeast)
    

    Use

    Synthetic Biology

    Tags

    mVenus-FLAG

    Expression

    Yeast

    Mutation

    Set2 point mutations K538G, K539S, R549G, K550S, …

    Promoter

    ADH1

    Available since

    June 2, 2021

    Availability

    Academic Institutions and Nonprofits only

    

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21. pEGFP-RNF138-delta 86-105

    Plasmid

    #78922

    Purpose

    Mammalian expression of RNF138 with delta 86-105 and an EGFP fusion

    Depositor

    Michael Hendzel

    Article

    Ismail et al Nat Cell Biol. 2015 Nov;17(11):1446-57.

    Insert

    RNF138 (RNF138 Human)
    

    Use

    Tags

    EGFP

    Expression

    Mammalian

    Mutation

    delta 86-105

    Promoter

    CMV

    Available since

    June 27, 2016

    Availability

    Academic Institutions and Nonprofits only

22. pEGFP-RNF138-delta 158-180

    Plasmid

    #78923

    Purpose

    Mammalian expression of RNF138 with delta 158-180 and an EGFP fusion

    Depositor

    Michael Hendzel

    Article

    Ismail et al Nat Cell Biol. 2015 Nov;17(11):1446-57.

    Insert

    RNF138 (RNF138 Human)
    

    Use

    Tags

    EGFP

    Expression

    Mammalian

    Mutation

    delta 159-180

    Promoter

    CMV

    Available since

    June 27, 2016

    Availability

    Academic Institutions and Nonprofits only

23. pEGFP-RNF138-delta 189-215

    Plasmid

    #78924

    Purpose

    Mammalian expression of RNF138 with delta 189-215 and an EGFP fusion

    Depositor

    Michael Hendzel

    Article

    Ismail et al Nat Cell Biol. 2015 Nov;17(11):1446-57.

    Insert

    RNF138 (RNF138 Human)
    

    Use

    Tags

    EGFP

    Expression

    Mammalian

    Mutation

    delta 189-215

    Promoter

    CMV

    Available since

    June 27, 2016

    Availability

    Academic Institutions and Nonprofits only

24. Flag-M4-AR-W741C-S81A

    Plasmid

    #171248

    Purpose

    Mammalian expression of 3xFlag-tagged human androgen receptor phosphorylation mutant that binds to bicalutamide: AR-Trp741Cys-Ser81Ala (AR-W741C-S81A)

    Depositor

    Steven Balk

    Article

    Chen et al J Biol Chem. 2012 Mar 9;287(11):8571-83.

    Insert

    3xFlag-tagged human androgen receptor Trp741Cys-Ser81Ala mutant (AR Human)
    

    Use

    Tags

    Flag

    Expression

    Mammalian

    Mutation

    AR-Trp741Cys-Ser81Ala (AR-W741C-S81A)

    Promoter

    CMV

    Available since

    June 25, 2021

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

25. Flag-M4-AR-W741C-S81D

    Plasmid

    #171249

    Purpose

    Mammalian expression of 3xFlag-tagged human androgen receptor phosphorylation mutant that binds to bicalutamide: AR-Trp741Cys-Ser81Asp (AR-W741C-S81D)

    Depositor

    Steven Balk

    Article

    Chen et al J Biol Chem. 2012 Mar 9;287(11):8571-83.

    Insert

    3xFlag-tagged human androgen receptor Trp741Cys-Ser81Asp mutant (AR Human)
    

    Use

    Tags

    Flag

    Expression

    Mammalian

    Mutation

    AR-Trp741Cys-Ser81Asp (AR-W741C-S81D)

    Promoter

    CMV

    Available since

    June 25, 2021

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

26. pCAG-FLAG-IRES-blasticidin-Sox2 S248A/T258A

    Plasmid

    #182049

    Purpose

    expression of Sox2 mutants

    Depositor

    Hyonchol Jang

    Article

    Kim et al Exp Mol Med. 2021 Nov;53(11):1759-1768.

    Insert

    Sox2 (Sox2 Mouse)
    

    Use

    Tags

    FLAG

    Expression

    Mammalian

    Mutation

    S248A/T258A

    Promoter

    CAG

    Available since

    April 13, 2022

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

27. pLenti-Puro-sgMARK4

    Plasmid

    #138691

    Purpose

    Expresses a human MARK4-targeting sgRNA and Cas9

    Depositor

    Reuben Shaw

    Article

    Hollstein et al Cancer Discov. 2019 Nov;9(11):1606-1627.

    Insert

    sgMARK4 human (MARK4 Human)
    

    Use

    Lentiviral

    Tags

    Expression

    Mutation

    Promoter

    hU6

    Available since

    Feb. 26, 2020

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

28. MAC-CLEC4D

    Plasmid

    #172411

    Purpose

    MAC-tagged gene expression

    Depositor

    Markku Varjosalo

    Article

    Liu et al Mol Syst Biol. 2021 Nov;17(11):e10396. d

    Insert

    C-type lectin domain family 4 member D (CD antigen CD368) (CLEC4D Human)
    

    Use

    Tags

    MAC-tag

    Expression

    Mammalian

    Mutation

    Promoter

    CMV promoter

    Available since

    Nov. 4, 2021

    Availability

    Industry, Academic Institutions, and Nonprofits

    

    Enlarge

29. pGL3-DICER-Prom Ebox double mut

    Plasmid

    #25854

    Depositor

    David E. Fisher

    Article

    Levy et al Cell. 2010 Jun 11. 141(6):994-1005.

    Insert

    DICER-Promoter Ebox double mut (DICER1 gene ID 23405, Human)
    

    Use

    Luciferase

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    Available since

    Aug. 25, 2010

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

30. pGEX-6P-1 p37 SHPmut

    Plasmid

    #113503

    Purpose

    Bacterial expression of GST-tagged p37 that is p97 binding deficient due to mutations in the SHP box

    Depositor

    Hemmo Meyer

    Article

    Weith et al Mol Cell. 2018 Oct 11. pii: S1097-2765(1

    Insert

    p37 (Ubxn2b Mouse)
    

    Use

    Tags

    GST-PreScission protease site

    Expression

    Bacterial

    Mutation

    F215A/E218A/QKL220-222AAA

    Promoter

    Available since

    Oct. 11, 2018

    Availability

    Academic Institutions and Nonprofits only

    

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