We narrowed to 5,762 results for: Chia
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Plasmid#222728PurposepNP561 - T5 Target with T7 Inducible IS621 RecombinaseDepositorInsertIS621 Recombinase
ExpressionBacterialAvailable SinceJuly 24, 2024AvailabilityAcademic Institutions and Nonprofits only -
pSLS.492
Plasmid#184957PurposeTest effect of a1/a2 length on editing rpoB using retron recombineeringDepositorInsertEco1 RT and recombineering ncRNA, rpoB T1534C, a1/a2 length: 22
ExpressionBacterialMutationrpoB donor T1534C, a1/a2 length extended to 22 bpPromoterT7/lacAvailable SinceNov. 4, 2022AvailabilityAcademic Institutions and Nonprofits only -
pET-MCN-10His-StayGold
Plasmid#211360PurposeBacterial expression of the bright and photostable StayGold fluorescent protein. Codon optimised for expression in Escherichia coli. Contains a N-terminal 10xHis tag.DepositorInsertStayGold
Tags10xHis-tagExpressionBacterialPromoterT7Available SinceDec. 21, 2023AvailabilityAcademic Institutions and Nonprofits only -
pCx55.5-TrpR
Plasmid#86373Purposefor zebrafish transgenesis, expression of TrpR from Cx55.5 promoter, also expresses mCherryDepositorInsertTrpR
UseZebrafishPromotercx55.5Available SinceAug. 17, 2017AvailabilityAcademic Institutions and Nonprofits only -
p201H Cas9
Plasmid#59176PurposeCas9 driven by double 35S, hygromycin resistance for plant selection, I-PpoI site to accept gRNA from pUC gRNA ShuttleDepositorInsertsCas9
hph
UseCRISPRPromoter2x35S and StUbi-3PAvailable SinceAug. 20, 2014AvailabilityAcademic Institutions and Nonprofits only -
pNP2157
Plasmid#222732PurposepNP1950 - J23119-IS621 bRNA TBL-WT, DBL-WT, HDV; T7-IS621 RecombinaseDepositorInsertIS621 Helper (WT)
ExpressionBacterialAvailable SinceJuly 24, 2024AvailabilityAcademic Institutions and Nonprofits only -
pET28a_T7-ARG2
Plasmid#106474PurposeExpresses acoustic reporter genes or gas vesicles (ARG2) in Escherichia coliDepositorInsertARG2
UseSynthetic BiologyExpressionBacterialPromoterT7Available SinceMarch 23, 2018AvailabilityAcademic Institutions and Nonprofits only -
pSMART-SOS-GFPuv
Plasmid#102283PurposeFluorescent sensor of E. coli SOS responseDepositorInsertcda-GFPuv
ExpressionBacterialPromotercda SOS promoterAvailable SinceApril 12, 2018AvailabilityAcademic Institutions and Nonprofits only -
pMS82
Plasmid#68110PurposeIntegration vector derived from Streptomyces phage phiBT1. Hygromycin resistance gene, oriT, ColEi origin of replicationDepositorInsertsaac3(IV)
Hyg
oriT
attP
int
UseStreptomyces integrating vectorMutationFragmentAvailable SinceJuly 9, 2019AvailabilityAcademic Institutions and Nonprofits only -
pVS2
Plasmid#33144DepositorInsertsLacO (256 copies)
GAL-GFP-kd200-GALpA reporter
ExpressionYeastMutationGFP is mutated such that amino acids 64-73 (FSYGV…Promoterbidirectional GAL1-GAL10 promoterAvailable SinceDec. 20, 2011AvailabilityAcademic Institutions and Nonprofits only -
pBEL1092
Plasmid#195635PurposeExpression/purification of 6xHis-TEV-sfGFPDepositorInsertsfGFP
ExpressionBacterialAvailable SinceMay 2, 2023AvailabilityAcademic Institutions and Nonprofits only -
pBLADE_ONLY_A
Plasmid#168051PurposeExpresses the synthetic gene BLADE FP6 under the constitutive J23101** promoter.DepositorInsertBlue light-inducible AraC dimers in Escherichia coli
ExpressionBacterialPromoterJ23101**Available SinceMay 11, 2021AvailabilityAcademic Institutions and Nonprofits only -
pNP1047
Plasmid#222726PurposepNP555 - IS621 RE-LE TBL-T5, DBL-WT bRNA in cisDepositorInsertIS621 bridge RNA
TagssfGFPExpressionBacterialAvailable SinceJuly 24, 2024AvailabilityAcademic Institutions and Nonprofits only -
pSHDY-Prha-mVenus_rhaS
Plasmid#137662PurposeConjugative vector derived from pSHDY. Constitutive expression of the activator rhaS, as well as rhamnose-inducible expression of mVenus.DepositorInsertPrha:mVenus
ExpressionBacterialMutationrhaS (Arg218Leu)PromoterPrhaAvailable SinceFeb. 26, 2020AvailabilityAcademic Institutions and Nonprofits only -
pEvoltac-EcW-TGA-h14
Plasmid#218764Purposeencodes EcWRS-h14 and EcW-tRNA-TGADepositorInsertEcWRS-h14
ExpressionBacterialMutationS8A, V144G, V146CPromotertacIAvailable SinceJuly 25, 2024AvailabilityAcademic Institutions and Nonprofits only -
pG108-K - AhpC-Glow
Plasmid#185135PurposeBacteroides - Escherichia coli shuttle vector with TetQ and Kanamycin selection markers BS2 is cloned under the Bacteroides thetaiotaomicron ahpC promoterDepositorInsertsGlow gene
Bacteroides thetaiotaomicron ahpC promoter
UsePg106ExpressionBacterialAvailable SinceDec. 5, 2022AvailabilityAcademic Institutions and Nonprofits only -
Traptavidin-E6
Plasmid#59549PurposepET21-Tre for expression of traptavidin (high stability mutant of streptavidin) bearing a glutamic acid tag for ion-exchange chromatographyDepositorInsertCore Traptavidin-E6
TagsE6 tagExpressionBacterialPromoterT7Available SinceOct. 6, 2014AvailabilityAcademic Institutions and Nonprofits only -
pTXRE5-Z
Plasmid#112509PurposeReporter plasmid, responsive to activated Ah receptor/ArntDepositorInsertRW95-3 with 5x xenobiotic response elements (lacZ Synthetic, E.coli)
ExpressionYeastPromoter5 XREs+ minimal Cytochrome CAvailable SinceJuly 13, 2018AvailabilityAcademic Institutions and Nonprofits only -
pSLS.402
Plasmid#187202PurposeExpresses retron Eco1 RTDepositorInsertRetron Eco1 Reverse Transcriptase
UseSynthetic BiologyExpressionBacterialPromotermphRAvailable SinceSept. 28, 2022AvailabilityAcademic Institutions and Nonprofits only -
pBLADE_ONLY_C
Plasmid#168050PurposeExpresses the synthetic gene BLADE FP6 under the constitutive J23101** promoter.DepositorInsertBlue light-inducible AraC dimers in Escherichia coli
ExpressionBacterialPromoterJ23101**Available SinceMay 11, 2021AvailabilityAcademic Institutions and Nonprofits only -
SSB-wt
Plasmid#197260Purposewild type E. coli SSB in pET21 expresses in BL21 under T7 control with lactose-amp resistanceDepositorInsertwt E. coli SSB
TagsnoneExpressionBacterialPromoterT7Available SinceMarch 22, 2023AvailabilityAcademic Institutions and Nonprofits only -
mCherry-eDHFR
Plasmid#107266PurposemCherry-eDHFR in pIVT vector for in vitro transcriptionDepositorAvailable SinceMarch 5, 2019AvailabilityAcademic Institutions and Nonprofits only -
Skp
Plasmid#213139PurposeExpresses chaperone protein SKPDepositorAvailable SinceFeb. 7, 2024AvailabilityAcademic Institutions and Nonprofits only -
ABE-RA4.2
Plasmid#208307PurposeExpresses ABE-RA4.2 in mammalian cellsDepositorInsertTadA-RA4.2-SpCas9 D10A
ExpressionMammalianMutationP48A, R51H, I76F, A106V, D108G, K110R, T111H, D11…Available SinceJan. 2, 2024AvailabilityAcademic Institutions and Nonprofits only -
pHCKan-phoAp-GFPuv
Plasmid#139072PurposeLow phosphate inducible promoter phoAp for GFP expressionDepositorInsertphoAp-GFPuv
ExpressionBacterialAvailable SinceSept. 10, 2020AvailabilityAcademic Institutions and Nonprofits only -
pENT-L1-Kozak-lacZ-stop-L2
Plasmid#186359PurposeEntry clone with ORF encoding lacZ flanked by Gateway recombination sequences.DepositorInsertlacZ (lacZ Synthetic, E. coli)
UseExpression of lacz reporterAvailable SinceSept. 28, 2022AvailabilityAcademic Institutions and Nonprofits only -
pEMPTY::sgRNA2
Plasmid#165459PurposeEscherichia coli – Staphylococcus aureus shuttle vector for plasmid curing in Gram-positive bacteriaDepositorInsertsgRNA2
UseCRISPR and Synthetic BiologyExpressionBacterialPromoterSP01Available SinceMarch 10, 2021AvailabilityAcademic Institutions and Nonprofits only -
pRT802
Plasmid#68113PurposeIntegration vector derived from Streptomyces phage phiBT1. Kanamycin resistance gene, oriT, ColE1 origin of replication.DepositorInsertsaac3(IV)
aph11
oriT
attP
int
UseStreptomyces integrating vectorAvailable SinceJuly 9, 2019AvailabilityAcademic Institutions and Nonprofits only -
pJB042 (FtsZ-mEos2)
Plasmid#49764PurposeInducible expression of FtsZ-mEos2 in bacteriaDepositorInsertFtsZ-mEos2
TagsmEos2ExpressionBacterialPromoterT5-lacAvailable SinceMarch 7, 2014AvailabilityAcademic Institutions and Nonprofits only -
pBEL1284
Plasmid#175960PurposeFor expression of LetA-LetB operon from E. coli. N-terminal his tag on LetA, araBAD promoterDepositorInsertsLetA
LetB
Tags6xHis 2xQH and TEV cleavage site on LetAExpressionBacterialPromoteraraBADAvailable SinceOct. 3, 2022AvailabilityAcademic Institutions and Nonprofits only