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YFP-Sp100A(K297A)-C1

Plasmid

#134552

Purpose

Expresses YFP-Sp100A fusion protein with a K297A mutation

Depositor

Susan Janicki

Article

Newhart et al Mol Biol Cell. 2013 Mar 13.

Insert

Sp100A(K297A)

Use

Tags

YFP

Expression

Mammalian

Mutation

K297R point mutation in the SUMO site

Promoter

CMV

Available since

Feb. 10, 2020

Availability

Academic Institutions and Nonprofits only

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FLM-PC1

Plasmid

#41567

Depositor

Thomas Weimbs

Article

Talbot et al Proc Natl Acad Sci U S A. 2011 May 10;10

Insert

PKD1 (PKD1 Human)

Use

Tags

CD16.7

Expression

Mammalian

Mutation

sequence from 4078 - 4303 of PKD1

Promoter

Available since

March 28, 2013

Availability

Academic Institutions and Nonprofits only

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UAS:iGluSnfr.zf1

Plasmid

#108356

Purpose

UAS driven variant of iGluSnFR for zebrafish expression

Depositor

Harold Burgess

Article

Tabor et al Curr Biol. 2018 Aug 20;28(16):2527-2535.

Insert

UAS:[GlyRa1 secretion sequence]-[iGluSnFR]

Use

Zebrafish expression

Tags

Expression

Mutation

Promoter

lac

Available since

Oct. 5, 2018

Availability

Academic Institutions and Nonprofits only

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px459V2.0_Conc3

Plasmid

#134458

Purpose

Empty concatemer vector in which 3 sgRNAs can be inserted; with Cas9 + Puro

Depositor

Daniel Stange

Article

Gaebler et al J Vis Exp. 2020 Feb 18;(156). doi: 10.37

Type

Empty backbone

Use

CRISPR

Tags

Expression

Mammalian

Mutation

Promoter

U6

Available since

Jan. 21, 2020

Availability

Academic Institutions and Nonprofits only

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pSYC-97

Plasmid

#31565

Depositor

Seok-Yong Choi

Article

Kim et al PLoS One. 2011 . 6(4):e18556.

Insert

pCS4+-NLS-EGFP-P2A-mCherry-CAAX

Use

Tags

Expression

Mutation

Promoter

Available since

June 3, 2016

Availability

Academic Institutions and Nonprofits only

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CasRX_Pac

Plasmid

#194803

Purpose

CasRX vector for gene knockdown in Giardia. Insert gRNA using Bbs1 restriction site. Puromycin resistance cassette.

Depositor

Alexander Paredez

Article

Shih et al Proc Natl Acad Sci U S A. 2022 May 31;11

Insert

CasRX

Use

Tags

Expression

Mutation

Promoter

Available since

April 27, 2023

Availability

Academic Institutions and Nonprofits only

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pLNC Wnt-3a

Plasmid

#17994

Depositor

Jan Kitajewski

Article

Shimizu et al Cell Growth Differ. 1997 Dec . 8(12):134

Insert

Wnt-3a (Wnt3a Mouse)

Use

Retroviral

Tags

Expression

Mutation

Promoter

Available since

July 7, 2008

Availability

Academic Institutions and Nonprofits only
pMSCV-PIG-miR-150

Plasmid

#64235

Purpose

Expresses miR-150 in mammalian cells

Depositor

Joshua Mendell

Article

Chang et al Nat Genet. 2008 Jan;40(1):43-50. Epub 20

Insert

miR150 (MIR150 Human)

Use

Retroviral

Tags

Expression

Mammalian

Mutation

Promoter

Available since

June 26, 2015

Availability

Academic Institutions and Nonprofits only

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NTS-PC1

Plasmid

#41574

Depositor

Thomas Weimbs

Article

Talbot et al Proc Natl Acad Sci U S A. 2011 May 10;10

Insert

PKD1 (PKD1 Human)

Use

Tags

CD16.7 and Myc/Myc/His

Expression

Mammalian

Mutation

Promoter

Available since

March 28, 2013

Availability

Academic Institutions and Nonprofits only
pALPS puro miR30-L1221

Plasmid

#101335

Purpose

negative control for knockdowns target site: CTTGTCGATGAGAGCGTTTGT

Depositor

Jeremy Luban

Article

McCauley et al Nat Commun. 2018 Dec 13;9(1):5305. doi:

Insert

shRNA targeting miR30

Use

Lentiviral

Tags

Expression

Mutation

Promoter

Available since

March 14, 2019

Availability

Academic Institutions and Nonprofits only

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mSA-phi29

Plasmid

#210815

Purpose

in vitro transcription of mRNA

Depositor

Erik Sontheimer

Article

Liu et al Nat Biotechnol. 2023 Sep 14. doi: 10.103

Insert

mSA-Phi29

Use

Tags

Expression

Bacterial

Mutation

Promoter

Available since

Feb. 28, 2024

Availability

Academic Institutions and Nonprofits only

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pYR14 (XII-5:TDH3p:his6-mKate2-LipPks1-IDP1t)

Plasmid

#167357

Purpose

Integrative plasmid for cassette TDH3p:his6-mKate2-LipPks1-IDP1t

Depositor

Michael Jensen

Article

Romero-Suarez et al ACS Synth Biol. 2021 Feb 12. doi: 10.102

Insert

TDH3p:his6-mKate2-LipPks1-IDP1t

Use

Unspecified

Tags

Expression

Mutation

Promoter

Available since

April 13, 2021

Availability

Academic Institutions and Nonprofits only

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YFP-Sp100C(W655Q)-C3

Plasmid

#134556

Purpose

Expresses YFP-Sp100C fusion protein with a W655Q mutation

Depositor

Susan Janicki

Article

Newhart et al Mol Biol Cell. 2013 Mar 13.

Insert

Sp100C(W655Q)

Use

Tags

YFP

Expression

Mammalian

Mutation

W655Q point mutation in the SAND domain

Promoter

CMV

Available since

Feb. 10, 2020

Availability

Academic Institutions and Nonprofits only

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2 gRNA concatmer

Plasmid

#84879

Purpose

Empty concatmer vector in which 2 gRNAs can be inserted

Depositor

Bon-Kyoung Koo

Article

Andersson-Rolf et al Dev Biol. 2016 Oct 27. pii: S0012-1606(1

Type

Empty backbone

Use

Retroviral

Tags

Expression

Mammalian

Mutation

Promoter

Available since

June 12, 2017

Availability

Academic Institutions and Nonprofits only
mycUBB+1

Plasmid

#20254

Depositor

Nico Dantuma

Article

Verhoef et al FASEB J. 2009 Jan . 23(1):123-33.

Insert

Ubiquitin B +1 transcript (UBB Human)

Use

Tags

myc

Expression

Mammalian

Mutation

Promoter

Available since

April 14, 2009

Availability

Academic Institutions and Nonprofits only

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Phi3-iRFP

Plasmid

#106296

Purpose

CMV driven iRFP with n terminal membrane binding domain of GAP43

Depositor

Sanford Simon

Article

Belote et al J Cell Biol. 2020 Jan 6;219(1). pii: 132

Insert

iRFPmem

Use

Lentiviral

Tags

Expression

Mammalian

Mutation

Promoter

CMV

Available since

March 22, 2022

Availability

Academic Institutions and Nonprofits only

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CTS-PC1

Plasmid

#41576

Depositor

Thomas Weimbs

Article

Talbot et al Proc Natl Acad Sci U S A. 2011 May 10;10

Insert

PKD1 (PKD1 Human)

Use

Tags

CD16.7 and myc/myc/his

Expression

Mammalian

Mutation

FLS (4184-4303)

Promoter

Available since

March 28, 2013

Availability

Academic Institutions and Nonprofits only

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Rictor-Myc

Plasmid

#157931

Purpose

Over expression of Rictor

Depositor

Baoji Xu

Article

Xu et al Nat Commun. 2019 Aug 9;10(1):3622. doi:

Insert

Rictor (Rictor Mouse)

Use

Lentiviral

Tags

His and Myc

Expression

Mammalian

Mutation

Promoter

Ubiquitinc Promoter

Available since

Sept. 10, 2020

Availability

Academic Institutions and Nonprofits only

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pcDNA6-MRPS16-VN172

Plasmid

#182375

Purpose

mVenus-based mito-riboBiFC vector (Negative control)

Depositor

Chunghun Lim

Article

Lee et al BMC Biol. 2022 Jan 7;20(1):12. doi: 10.1

Insert

Mrps16 (Mrps16 Mouse)

Use

Tags

VN172

Expression

Mammalian

Mutation

Promoter

CMV promoter

Available since

March 30, 2022

Availability

Academic Institutions and Nonprofits only

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p5E-mcs2

Plasmid

#138362

Purpose

Gateway compatible 5' entry clone contains mutli-cloning sites sequence2.

Depositor

Nathan Lawson

Article

Sissaoui et al Circ Res. 2020 Feb 17. doi: 10.1161/CIRC

Insert

sequence multi cloning sites

Use

Tags

Expression

Mammalian

Mutation

Promoter

Available since

Feb. 21, 2020

Availability

Academic Institutions and Nonprofits only

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pCRISPomyces-Sth1Cas9

Plasmid

#129552

Purpose

Contains codon-optimized Sth1Cas9 and gRNA for streptomyces

Depositor

Mingzi M. Zhang, Fong Tian Wong

Article

Yeo et al Biotechnol Bioeng. 2019 May 15. doi: 10.

Insert

Sth1Cas9

Use

Tags

Expression

Bacterial

Mutation

Promoter

rpsL(XC)-BbsI

Available since

Oct. 8, 2019

Availability

Academic Institutions and Nonprofits only

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pYR11 (XII-5:TDH3p:his6-mKate2-MBP-IDP1t)

Plasmid

#167356

Purpose

Integrative plasmid for cassette TDH3p:his6-mKate2-MBP-IDP1t

Depositor

Michael Jensen

Article

Romero-Suarez et al ACS Synth Biol. 2021 Feb 12. doi: 10.102

Insert

TDH3p:his6-mKate2-MBP-IDP1t

Use

Unspecified

Tags

Expression

Mutation

Promoter

Available since

April 13, 2021

Availability

Academic Institutions and Nonprofits only

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pJKR-O-acuR

Plasmid

#62569

Purpose

Acrylate biosensor. Medium copy plasmid that expresses CFP proportional to acrylate concentration. Compatible with all other pJKR plasmids.

Depositor

George Church

Article

Rogers et al Nucleic Acids Res. 2015 Jul 7. pii: gkv6

Insert

CFP

Use

Tags

Expression

Bacterial

Mutation

Promoter

Available since

Aug. 13, 2015

Availability

Academic Institutions and Nonprofits only

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3494 pcDNA3 Bax

Plasmid

#8779

Depositor

Stanley Korsmeyer

Article

Oltvai et al Cell 1993 Aug 27;74(4):609-19.

Insert

Bax (Bax Mouse)

Use

Tags

Expression

Mammalian

Mutation

Promoter

Available since

Aug. 4, 2006

Availability

Academic Institutions and Nonprofits only
USP19 sgRNA1

Plasmid

#78585

Purpose

delete USP19 gene in human cell

Depositor

Yihong Ye

Article

Lee et al Nat Cell Biol. 2016 Jul;18(7):765-76. do

Insert

USP19 sgRNA1

Use

CRISPR

Tags

Expression

Mammalian

Mutation

Promoter

CBh

Available since

June 29, 2016

Availability

Academic Institutions and Nonprofits only
pJYDNgp

Plasmid

#162453

Purpose

Positive control plasmid for pJYDNg carrying the eUnaG2 bilirubin dependent fluorescent protein fused to the C-terminus of Aga2p protein. This plasmid contains a long 2G linker.

Depositor

Gideon Schreiber

Article

Zahradník et al bioRxiv Preprint 2020

Insert

Aga2p-2Glinker-eUnaG2 (AGA2 )

Use

Shuttle vector bacteria/yeast

Tags

HA nad myc and eUnaG2 fluorescent protein

Expression

Mutation

Promoter

GAL1

Available since

Feb. 19, 2021

Availability

Academic Institutions and Nonprofits only

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pSUPER.PKD3.RNAi

Plasmid

#10818

Depositor

Alex Toker

Article

Hausser et al Nat Cell Biol. 2005 Sep . 7(9):880-6.

Insert

PKD3 RNAi (PRKD3 Human)

Use

RNAi

Tags

Expression

Mammalian

Mutation

Promoter

Available since

Oct. 6, 2005

Availability

Academic Institutions and Nonprofits only
pUdOm1.0

Plasmid

#210298

Purpose

Empty backbone with ampicillin resistance for high expression in mammalian cells with an added 5'UTR

Depositor

Corrie daCosta

Article

Manigat et al ACS Synth Biol. 2024 Jan 18. doi: 10.102

Type

Empty backbone

Use

Synthetic Biology

Tags

Expression

Mammalian

Mutation

Promoter

CMV

Available since

Sept. 10, 2024

Availability

Academic Institutions and Nonprofits only

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pUE1-TSP-UL32

Plasmid

#162658

Purpose

Expresses UL32 in mammalian cells with an N-terminal TwinStrep tag and HRV 3C protease cleavage site

Depositor

Britt Glaunsinger

Article

Didychuk et al Elife. 2021 Feb 8;10. pii: 62261. doi: 1

Insert

UL32

Use

Tags

N-terminal 2xStrep-Tag II and HRV 3C site

Expression

Mammalian

Mutation

Promoter

CMV

Available since

Feb. 17, 2021

Availability

Academic Institutions and Nonprofits only

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pMKO.1-Puro-shPP2A-Aalpha

Plasmid

#15249

Depositor

William Hahn

Article

Sablina et al Cell. 2007 Jun 1. 129(5):969-82.

Insert

PP2A regulatory subunit A, alpha isoform (PPP2R1A Human)

Use

RNAi and Retroviral

Tags

Expression

Mammalian

Mutation

Promoter

Available since

July 12, 2007

Availability

Academic Institutions and Nonprofits only