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Plasmid

#158620

Purpose

DNA fragment production for nucleosome core particle preparation

Depositor

Curt Davey

Article

Vasudevan et al J Mol Biol. 2010 Oct 15;403(1):1-10. doi

Type

Empty backbone

Use

Bacterial vector for dna production

Available Since

Nov. 13, 2020

Availability

Academic Institutions and Nonprofits only

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pFH17

Plasmid

#125803

Purpose

Level0 LbCas12a, rice codon optimized

Depositor

Vladimir Nekrasov

Article

Hahn et al BMC Plant Biol. 2020 Apr 23;20(1):179. d

Insert

LbCas12a, rice codon optimized

Use

CRISPR and Synthetic Biology

Expression

Plant

Available Since

Aug. 19, 2019

Availability

Academic Institutions and Nonprofits only

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pGEX4T1-Smurf1 C699A

Plasmid

#13503

Depositor

Jeff Wrana

Article

Zhu et al Nature. 1999 Aug 12. 400(6745):687-93.

Insert

SMURF1 (SMURF1 Human)

Tags

GST

Expression

Bacterial

Mutation

C699A: catalytically inactive

Available Since

Dec. 4, 2006

Availability

Industry, Academic Institutions, and Nonprofits
B6-3

Plasmid

#111487

Purpose

Ser-tRNA synthetase

Depositor

Cheemeng Tan

Article

Villarreal et al Nat Chem Biol. 2018 Jan;14(1):29-35. doi

Insert

Ser-tRNA synthetase

Tags

6xHis

Expression

Bacterial

Available Since

Sept. 14, 2018

Availability

Academic Institutions and Nonprofits only

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pCDF_HA_CASP_sigma

Plasmid

#197614

Purpose

For expression of HA-tagged CASP sigma in E. coli

Depositor

Feng Zhang

Article

Strecker et al Science. 2022 Nov 25;378(6622):874-881.

Insert

HA-tagged CASP sigma

Expression

Bacterial

Available Since

June 13, 2023

Availability

Academic Institutions and Nonprofits only

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pCMV-GFP-CENPBpA

Plasmid

#120162

Purpose

EGFP followed by the poly(A) site from human CENPB gene

Depositor

David Bartel

Article

Wu et al Cell. 2017 May 18;169(5):905-917.e11. do

Insert

CENPB poly(A) site

Expression

Mammalian

Promoter

CMV

Available Since

Feb. 12, 2020

Availability

Academic Institutions and Nonprofits only

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pAK-EL-01

Plasmid

#125760

Purpose

Endlinker for tRNA-gRNA system when using only one guide

Depositor

Vladimir Nekrasov

Article

Hahn et al BMC Plant Biol. 2020 Apr 23;20(1):179. d

Insert

Endlinker

Use

CRISPR and Synthetic Biology

Expression

Plant

Available Since

Aug. 19, 2019

Availability

Academic Institutions and Nonprofits only

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pAK-EL-04

Plasmid

#125763

Purpose

Endlinker for tRNA-gRNA system when using only four guides

Depositor

Vladimir Nekrasov

Article

Hahn et al BMC Plant Biol. 2020 Apr 23;20(1):179. d

Insert

Endlinker

Use

CRISPR and Synthetic Biology

Expression

Plant

Available Since

Aug. 19, 2019

Availability

Academic Institutions and Nonprofits only

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pAK-EL-05

Plasmid

#125764

Purpose

Endlinker for tRNA-gRNA system when using only five guides

Depositor

Vladimir Nekrasov

Article

Hahn et al BMC Plant Biol. 2020 Apr 23;20(1):179. d

Insert

Endlinker

Use

CRISPR and Synthetic Biology

Expression

Plant

Available Since

Aug. 19, 2019

Availability

Academic Institutions and Nonprofits only

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pAL197

Plasmid

#22280

Depositor

Christopher Voigt

Article

Levskaya et al Nature. 2009 Oct 15;461(7266):997-1001.

Insert

p!SV40-PakGBD-mCherry

Use

Synthetic Biology

Expression

Mammalian

Available Since

Oct. 21, 2009

Availability

Academic Institutions and Nonprofits only

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pPI896

Plasmid

#207453

Purpose

REMI integrated Expression for mNeonGreen-Nap1

Depositor

Rob Kay

Article

Lutton et al Curr Biol. 2023 Aug 7;33(15):3083-3096.e

Insert

Nap1

Use

Unspecified

Tags

mNeonGreen

Available Since

Dec. 4, 2023

Availability

Academic Institutions and Nonprofits only

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B5-2

Plasmid

#111481

Purpose

Arg-tRNA synthetase

Depositor

Cheemeng Tan

Article

Villarreal et al Nat Chem Biol. 2018 Jan;14(1):29-35. doi

Insert

Arg-tRNA synthetase

Tags

6xHis

Expression

Bacterial

Available Since

Jan. 8, 2019

Availability

Academic Institutions and Nonprofits only

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GST-14-3-3dm (pEBG)

Plasmid

#1943

Depositor

Joseph Avruch

Article

Tzivion et al Nature 1998 Jul 2;394(6688):88-92.

Insert

14-3-3 zeta (Ywhaz Mouse)

Tags

GST

Expression

Mammalian

Mutation

Dimerization-deficient mutant of 14-3-3zeta aa: E…

Available Since

April 20, 2006

Availability

Academic Institutions and Nonprofits only
pAK-EL-02

Plasmid

#125761

Purpose

Endlinker for tRNA-gRNA system when using only two guides

Depositor

Vladimir Nekrasov

Article

Hahn et al BMC Plant Biol. 2020 Apr 23;20(1):179. d

Insert

Endlinker

Use

CRISPR and Synthetic Biology

Expression

Plant

Available Since

Aug. 19, 2019

Availability

Academic Institutions and Nonprofits only

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NM R(delta) 2-5 GFP

Plasmid

#1209

Depositor

Susan Lindquist

Article

Liu et al Nature 1999 Aug 5;400(6744):573-6.

Insert

sup35 NM domain (SUP35 Budding Yeast)

Tags

GFP

Expression

Yeast

Mutation

last four repeats of sup35 N domain deleted

Available Since

May 25, 2005

Availability

Academic Institutions and Nonprofits only

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B3-3

Plasmid

#111473

Purpose

Glu-tRNA synthetase

Depositor

Cheemeng Tan

Article

Villarreal et al Nat Chem Biol. 2018 Jan;14(1):29-35. doi

Insert

Glu-tRNA synthetase

Tags

6xHis

Expression

Bacterial

Available Since

June 19, 2018

Availability

Academic Institutions and Nonprofits only

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pRK5-mWnt8b

Plasmid

#42285

Depositor

Chris Garcia, Jeremy Nathans

Article

Yu et al Development. 2012 Dec 1;139(23):4383-94.

Insert

Wnt8b (Wnt8b Mouse)

Expression

Mammalian

Available Since

March 28, 2013

Availability

Academic Institutions and Nonprofits only

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pCYP03

Plasmid

#212836

Purpose

Expresses the Acidovorax avenae subsp. Avenae LRD-modifying cytochrome P450 enzyme

Depositor

Christopher Voigt

Article

Lin et al Nature Catalysis. 6, 1016–1029 (2023). h

Insert

cytochrome P450

Expression

Bacterial

Promoter

PcymRC

Available Since

Feb. 7, 2024

Availability

Academic Institutions and Nonprofits only

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B2-3

Plasmid

#111470

Purpose

Lys-tRNA synthetase

Depositor

Cheemeng Tan

Article

Villarreal et al Nat Chem Biol. 2018 Jan;14(1):29-35. doi

Insert

Lys-tRNA synthetase

Tags

6xHis

Expression

Bacterial

Available Since

July 18, 2018

Availability

Academic Institutions and Nonprofits only

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B5-4

Plasmid

#111483

Purpose

Trp-tRNA synthetase

Depositor

Cheemeng Tan

Article

Villarreal et al Nat Chem Biol. 2018 Jan;14(1):29-35. doi

Insert

Trp-tRNA synthetase

Tags

5xHis

Expression

Bacterial

Available Since

Sept. 14, 2018

Availability

Academic Institutions and Nonprofits only

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tet operator

Plasmid

#8901

Depositor

Lynda Chin

Article

Chin et al Nature. 1999 Jul 29. 400(6743):468-72.

Insert

tet operator

Available Since

Jan. 5, 2006

Availability

Academic Institutions and Nonprofits only
pFH56

Plasmid

#125882

Purpose

Level0 Rice OsACT1 Pol II promoter

Depositor

Vladimir Nekrasov

Article

Hahn et al BMC Plant Biol. 2020 Apr 23;20(1):179. d

Insert

Level0 Rice OsAct1 Pol II promoter

Use

CRISPR and Synthetic Biology

Expression

Plant

Available Since

Aug. 19, 2019

Availability

Academic Institutions and Nonprofits only

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B2329_mCherry-FKBP2x

Plasmid

#215103

Purpose

Expresses mCherry-FKBP-FKBP in mammalian cells

Depositor

Chandra Tucker

Article

Hernandez-Candia et al Nat Chem Biol. 2024 Jan 8. doi: 10.1038/

Insert

mCherry-FKBP-FKBP

Tags

mCherry

Expression

Mammalian

Promoter

CMV

Available Since

March 19, 2024

Availability

Academic Institutions and Nonprofits only

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pMXs-Ecat8

Plasmid

#13357

Depositor

Shinya Yamanaka

Article

Takahashi et al Cell. 2006 Aug 25. 126(4):663-76.

Insert

ES cell associated transcript 8 (Tdrd12 Mouse)

Use

Retroviral

Expression

Mammalian

Mutation

None

Available Since

Jan. 12, 2007

Availability

Academic Institutions and Nonprofits only
B2-2

Plasmid

#111469

Purpose

Thr-tRNA synthetase

Depositor

Cheemeng Tan

Article

Villarreal et al Nat Chem Biol. 2018 Jan;14(1):29-35. doi

Insert

Thr-tRNA synthetase

Tags

6xHis

Expression

Bacterial

Available Since

June 19, 2018

Availability

Academic Institutions and Nonprofits only

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B4-2

Plasmid

#111476

Purpose

Asp-tRNA synthetase

Depositor

Cheemeng Tan

Article

Villarreal et al Nat Chem Biol. 2018 Jan;14(1):29-35. doi

Insert

Asp-tRNA synthetase

Tags

6xHis

Expression

Bacterial

Available Since

June 19, 2018

Availability

Academic Institutions and Nonprofits only

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B5-3

Plasmid

#111482

Purpose

Cys-tRNA synthetase

Depositor

Cheemeng Tan

Article

Villarreal et al Nat Chem Biol. 2018 Jan;14(1):29-35. doi

Insert

Cys-tRNA synthetase

Tags

6xHis

Expression

Bacterial

Available Since

June 19, 2018

Availability

Academic Institutions and Nonprofits only

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B8-3

Plasmid

#111495

Purpose

His-tRNA synthetase

Depositor

Cheemeng Tan

Article

Villarreal et al Nat Chem Biol. 2018 Jan;14(1):29-35. doi

Insert

His-tRNA synthetase

Tags

6xHis

Expression

Bacterial

Available Since

June 18, 2018

Availability

Academic Institutions and Nonprofits only

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B8-1

Plasmid

#111494

Purpose

Gly-tRNA synthetase B

Depositor

Cheemeng Tan

Article

Villarreal et al Nat Chem Biol. 2018 Jan;14(1):29-35. doi

Insert

Gly-tRNA synthetase B

Tags

6xHis

Expression

Bacterial

Available Since

June 18, 2018

Availability

Academic Institutions and Nonprofits only

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B4-3

Plasmid

#111477

Purpose

Asn-tRNA synthetase

Depositor

Cheemeng Tan

Article

Villarreal et al Nat Chem Biol. 2018 Jan;14(1):29-35. doi

Insert

Asn-tRNA synthetase

Tags

6xHis

Expression

Bacterial

Available Since

June 18, 2018

Availability

Academic Institutions and Nonprofits only

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